An N-terminal mutation in the bacteriophage T4 motA gene yields a protein that binds DNA but is defective for activation of transcription.

abstract：:The expression of cell cycle events in Caulobacter crescentus CB13 has been shown to be associated with regulation of carbohydrate utilization. Growth on lactose and galactose depends on induction of specific enzymes. Prior growth on glucose results in a delay in enzyme expression and cell cycle arrest at the nonmotil...

journal_title：Journal of bacteriology

authors： Kurn N,Shapiro L,Agabian N

更新日期：1977-09-01 00:00:00

abstract：:Antibiotic resistance in Neisseria gonorrhoeae has been associated with the acquisition of R plasmids from heterologous organisms. The broad-host-range plasmids of incompatibility groups P (IncP) and Q (IncQ) have played a role in this genetic exchange in nature. We have utilized derivatives of RSF1010 (IncQ) and RP1 ...

journal_title：Journal of bacteriology

authors： Butler CA,Gotschlich EC

更新日期：1991-09-01 00:00:00

abstract：:The effects of variations in growth conditions on the penicillin response of Streptococcus faecium ATCC 9790 were studied. Changes in the growth temperature and medium composition were found to cause striking changes in the bacterial generation time, cellular penicillin sensitivity (minimum inhibitory concentration), ...

journal_title：Journal of bacteriology

authors： Fontana R,Canepari P,Satta G,Coyette J

更新日期：1983-05-01 00:00:00

abstract：:In previous work, we cloned a Candida albicans cDNA for the glycolytic enzyme enolase and found a single, abundant enolase transcript on Northern (RNA) blots and a single protein on immunoblots, using antiserum raised against a recombinant enolase fusion protein. Because C. albicans enolase is abundantly produced duri...

journal_title：Journal of bacteriology

authors： Postlethwait P,Sundstrom P

更新日期：1995-04-01 00:00:00

abstract：:Expression of the hurIR bhuRSTUV heme utilization locus in Bordetella bronchiseptica is coordinately controlled by the global iron-dependent regulator Fur and the extracytoplasmic function sigma factor HurI. Activation of HurI requires transduction of a heme-dependent signal via HurI, HurR, and BhuR, a three-component...

journal_title：Journal of bacteriology

authors： King-Lyons ND,Smith KF,Connell TD

更新日期：2007-09-01 00:00:00

abstract：:Membrane fragments containing diacyl phospholipids were released from viable cells of Haemophilus parainfluenzae during incubation in ethylenediaminetetraacetic acid (EDTA)- tris(hydroxymethyl)aminomethane (Tris) buffer. The phospholipids located in the part of the membrane that was released during the EDTA-Tris treat...

journal_title：Journal of bacteriology

authors： Tucker AN,White DC

更新日期：1970-08-01 00:00:00

abstract：:A 3.6-kb EcoRI fragment containing the ntrA gene of Agrobacterium tumefaciens was cloned by using the homologous ntrA gene of Rhizobium meliloti as a probe. Construction of an ntrA mutant of A. tumefaciens by site-directed insertional mutagenesis demonstrated the requirement of the ntrA gene for nitrate utilization an...

journal_title：Journal of bacteriology

authors： Wu ZL,Charles TC,Wang H,Nester EW

更新日期：1992-04-01 00:00:00

abstract：:Huston, Charles K. (U.S. Army Biological Laboratories, Fort Detrick, Frederick, Md.), and Phillip W. Albro. Lipids of Sarcina lutea. I. Fatty acid composition of the extractable lipids. J. Bacteriol. 88:425-432. 1964.-The extractable lipids of Sarcina lutea were separated into several fractions by a combination of col...

journal_title：Journal of bacteriology

authors： HUSTON CK,ALBRO PW

更新日期：1964-08-01 00:00:00

abstract：:A system for high-level expression of heparinase I, heparinase II, heparinase III, chondroitinase AC, and chondroitinase B in Flavobacterium heparinum is described. hepA, along with its regulatory region, as well as hepB, hepC, cslA, and cslB, cloned downstream of the hepA regulatory region, was integrated in the chro...

journal_title：Journal of bacteriology

authors： Blain F,Tkalec AL,Shao Z,Poulin C,Pedneault M,Gu K,Eggimann B,Zimmermann J,Su H

更新日期：2002-06-01 00:00:00

abstract：:Legionella pneumophila is the causative agent of the severe and potentially fatal pneumonia Legionnaires' disease. L. pneumophila is able to replicate within macrophages and protozoa by establishing a replicative compartment in a process that requires the Icm/Dot type IVB secretion system. The signals and regulatory p...

journal_title：Journal of bacteriology

authors： Hovel-Miner G,Pampou S,Faucher SP,Clarke M,Morozova I,Morozov P,Russo JJ,Shuman HA,Kalachikov S

更新日期：2009-04-01 00:00:00

abstract：:The iota(a) component (i(a)) of Clostridium perfringens ADP ribosylates nonmuscle beta/gamma actin and skeletal muscle alpha-actin. Replacement of Arg-295 in i(a) with alanine led to a complete loss of NAD(+)-glycohydrolase (NADase) and ADP-ribosyltransferase (ARTase); that of the residue with lysine caused a drastic ...

journal_title：Journal of bacteriology

authors： Nagahama M,Sakaguchi Y,Kobayashi K,Ochi S,Sakurai J

更新日期：2000-04-01 00:00:00

abstract：:The cheF gene, which is involved in chemotaxis in Bacillus subtilis, has been cloned, expressed, and sequenced. This gene is contained in a 0.7-kilobase PstI DNA fragment that was isolated from a lambda Charon 4A B. subtilis chromosomal DNA library. This fragment was subcloned into the expression vector pSI-1 and show...

journal_title：Journal of bacteriology

authors： Ying CW,Ordal GW

更新日期：1989-03-01 00:00:00

abstract：:Certain group VIIIB transition metal compounds were found to inhibit cell division in Escherichia coli, causing marked filamentous growth. Gram-negative bacilli were the most sensitive to this effect, whereas gram-positive bacilli responded only at near-toxic levels of the metal. None of the cocci tested showed any ap...

journal_title：Journal of bacteriology

authors： Rosenberg B,Renshaw E,Vancamp L,Hartwick J,Drobnik J

更新日期：1967-02-01 00:00:00

abstract：:For the pathogenic yeast Candida albicans, secreted aspartyl proteinase (Sap) activity has been correlated with virulence. A family consisting of at least eight SAP genes can be drawn upon to produce Sap enzymatic activity. In this study, the levels of Sap1, Sap2, and Sap3 isoenzymes were monitored under a variety of ...

journal_title：Journal of bacteriology

authors： White TC,Agabian N

更新日期：1995-09-01 00:00:00

abstract：:A cyclic polyol, 1,5-anhydro-D-glucitol (AG), is widely detected in most organisms, although little is known about its metabolism and physiological roles. The present study demonstrates the synthesis of AG in Escherichia coli C600. The major portion of the synthesized AG was indicated to be derived from glucose retain...

journal_title：Journal of bacteriology

authors： Shiga Y,Mizuno H,Akanuma H

更新日期：1993-11-01 00:00:00

abstract：:Mir M7 is a spontaneous morphologically conditional mutant of Klebsiella pneumoniae which grows as round cells (cocci) at pH 7 and as normal rods at pH 5.8. We studied the rates of peptidoglycan synthesis of cocci and rods growing at pH values of 7 and 5.8, respectively. It was found that exponentially growing cocci p...

journal_title：Journal of bacteriology

authors： Satta G,Fontana R,Canepari P,Botta G

更新日期：1979-02-01 00:00:00

abstract：:Molybdenum (Mo) is an important trace element that is toxic at high concentrations. To resolve the mechanisms underlying Mo toxicity, Rhodobacter capsulatus mutants tolerant to high Mo concentrations were isolated by random transposon Tn5 mutagenesis. The insertion sites of six independent isolates mapped within the s...

journal_title：Journal of bacteriology

authors： Gisin J,Müller A,Pfänder Y,Leimkühler S,Narberhaus F,Masepohl B

更新日期：2010-11-01 00:00:00

abstract：:In streptococcal species, the key step of competence development is the transcriptional induction of comX, which encodes the alternative sigma factor sigma(X), which positively regulates genes necessary for DNA transformation. In Streptococcus species belonging to the mitis and mutans groups, induction of comX relies ...

journal_title：Journal of bacteriology

authors： Fontaine L,Boutry C,de Frahan MH,Delplace B,Fremaux C,Horvath P,Boyaval P,Hols P

更新日期：2010-03-01 00:00:00

abstract：:Sixteen independent Azorhizobium sesbaniae ORS571 vector insertion (Vi) mutants defective in ammonium assimilation (Asm-) were selected; genomic DNA sequences flanking the insertion endpoints were cloned directly. Resulting recombinant plasmids were used to identify, by hybridization, corresponding wild-type DNA seque...

journal_title：Journal of bacteriology

authors： Donald RG,Lapointe J,Ludwig RA

更新日期：1988-03-01 00:00:00

abstract：:Mutants defective in uptake or utilization of a given metabolite can readily be obtained from facultative auxotrophs (for that metabolite) by penicillin enrichment under nonpermissive conditions in the presence of a low level of the required metabolite. ...

journal_title：Journal of bacteriology

authors： Berg CM,Rossi JJ,Coleman JV,Conlon M

更新日期：1975-03-01 00:00:00

abstract：:Azotobacter vinelandii was grown diazotrophically in chemostat cultures limited by sucrose, citrate, or acetate. Specific activities of cellular oxygen consumption (qO2) and nitrogenase (acetylene reduction) were measured in situ at different dilution rates (D, representing the specific growth rate mu at steady state)...

journal_title：Journal of bacteriology

authors： Kuhla J,Oelze J

更新日期：1988-11-01 00:00:00

abstract：:A technique for identifying and characterizing staphylococcal hemolysins by first separating them electrophoretically in barbital-buffered agar gel (pH 8.4) at 5 ma/cm for 2 hr and then determining their hemolytic activities by exposing them to human, horse, rabbit, and sheep erythrocytes is described. The alpha-hemol...

journal_title：Journal of bacteriology

authors： Haque RU

更新日期：1967-02-01 00:00:00

abstract：:Fructose, galactose, L-arabinose, gluconate, and several organic acids support rapid growth and N2 fixation of Azospirillum brasiliense ATCC 29145 (strain Sp7) as a sole source of carbon and energy. Growth of Azospirillum lipoferum ATCC 29707 (strain Sp59b) is also supported by glucose, mannose, mannitol, and alpha-ke...

journal_title：Journal of bacteriology

authors： Martinez-Drets G,Del Gallo M,Burpee C,Burris RH

更新日期：1984-07-01 00:00:00

abstract：:Silicibacter sp. strain TM1040, a member of the Roseobacter clade, forms a symbiosis with unicellular phytoplankton, which is inextricably linked to the biphasic "swim or stick" lifestyle of the bacteria. Mutations in flaC bias the population toward the motile phase. Renewed examination of the FlaC(-) strain (HG1016) ...

journal_title：Journal of bacteriology

authors： Sule P,Belas R

更新日期：2013-02-01 00:00:00

abstract：:We previously described a gene, lpt3, required for the addition of phosphoethanolamine (PEtn) at the 3 position on the beta-chain heptose (HepII) of the inner-core Neisseria meningitidis lipopolysaccharide (LPS), but it has long been recognized that the inner-core LPS of some strains possesses PEtn at the 6 position (...

journal_title：Journal of bacteriology

authors： Wright JC,Hood DW,Randle GA,Makepeace K,Cox AD,Li J,Chalmers R,Richards JC,Moxon ER

更新日期：2004-10-01 00:00:00

abstract：:Respiration-deficient mutants (Rho-, petite) of Saccharomyces carlsbergensis were obtained by treatment with trypaflavin (euflavine). Dried cells of these mutants phosphorylated mononucleotides to their triphosphates and further formed not only cytidine 5'-diphosphate-choline, but also sugar nucleotides, such as uridi...

journal_title：Journal of bacteriology

authors： Kimura A,Hirose K,Kariya Y,Nagai S

更新日期：1976-02-01 00:00:00

abstract：:Plasmid R1 is a low-copy-number plasmid that is present at a level of about four or five copies per average cell. The copy number is controlled posttranscriptionally at the level of synthesis of the rate-limiting initiator protein RepA. In addition to this, R1 has an auxiliary system that derepresses a second promoter...

journal_title：Journal of bacteriology

authors： Olsson JA,Paulsson J,Nordström K

更新日期：2004-01-01 00:00:00

abstract：:Spore formation of Myxococcus xanthus can occur not only on agar plates during fruiting body formation, but also in a liquid culture by simply adding glycerol, dimethyl sulfoxide, or phenethyl alcohol to the culture. This chemically-induced spore formation occurs synchronously and much faster than that occurring durin...

journal_title：Journal of bacteriology

authors： Komano T,Inouye S,Inouye M

更新日期：1980-12-01 00:00:00

abstract：:Deletions of the Escherichia coli K-12 chromosome between trpR and thr render the bacterium sensitive to the dye toluidine blue (Dye-), and if male (Hfr or F'), the strain is sterile (Fex-), failing to donate F' or chromosomal markers and resistant to male-specific phages as a consequence of its inability to elaborate...

journal_title：Journal of bacteriology

authors： Buxton RS,Drury LS

更新日期：1983-06-01 00:00:00

abstract：:A gene (yacK) encoding a putative multicopper oxidase (MCO) was cloned from Escherichia coli, and the expressed enzyme was demonstrated to exhibit phenoloxidase and ferroxidase activities. The purified protein contained six copper atoms per polypeptide chain and displayed optical and electron paramagnetic resonance (E...

journal_title：Journal of bacteriology

authors： Kim C,Lorenz WW,Hoopes JT,Dean JF

更新日期：2001-08-01 00:00:00