Effect of the CopB auxiliary replication control system on stability of maintenance of Par(+) plasmid R1.

Abstract:

:Plasmid R1 is a low-copy-number plasmid that is present at a level of about four or five copies per average cell. The copy number is controlled posttranscriptionally at the level of synthesis of the rate-limiting initiator protein RepA. In addition to this, R1 has an auxiliary system that derepresses a second promoter at low copy numbers, leading to increased repA mRNA synthesis. This promoter is normally switched off by a constitutively synthesized plasmid-encoded repressor protein, CopB; in cells with low copy numbers, the concentration of CopB is low and the promoter is derepressed. Here we show that the rate of loss of a Par(+) derivative of the basic replicon of R1 increased about sevenfold when the cells contained a high concentration of the CopB protein formed from a compatible plasmid.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Olsson JA,Paulsson J,Nordström K

doi

10.1128/jb.186.1.207-211.2004

keywords:

subject

Has Abstract

pub_date

2004-01-01 00:00:00

pages

207-11

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

186

pub_type

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