Abstract:
:Uptake of (35)S-hydroxyethylthiazole (4-methyl-5-hydroxyethylthiazole) by Escherichia coli intact cells was studied. Hydroxyethylthiazole was taken up in the presence and absence of glucose at the same rate. The uptake was almost proportional to a hydroxyethylthiazole concentration gradient up to 0.1 mM with no tendency of saturation, and reached a steady state within 2 min. When the cells were treated with 1 mM N-ethylmaleimide, about 50% inhibition of hydroxyethylthiazole uptake was observed. Hydroxyethylthiazole uptake was stimulated by the addition of hydroxymethylpyrimidine (2-methyl-4-amino-5-hydroxymethylpyrimidine), and this effect was further enhanced in the presence of glucose. For full activation of hydroxyethylthiazole uptake, 1 muM hydroxymethylpyrimidine was necessary in the presence of glucose. The rate of hydroxyethylthiazole uptake was almost linear up to 60 min in the presence of hydroxymethylpyrimidine and glucose. Hydroxymethylpyrimidine monophosphate and its pyrophosphate could not stimulate the uptake. Thiamine and 2-amino-hydroxyethylthiazole were inhibitory on hydroxyethylthiazole uptake in the presence of hydroxymethylpyrimidine and glucose. N-ethylmaleimide and 2, 4-dinitrophenol were also inhibitory. No stimulatory effect of hydroxymethylpyrimidine on hydroxyethylthiazole uptake was observed in mutant cells lacking either thiaminephosphate pyrophosphorylase or hydroxymethylpyrimidine monophosphate kinase. The possibility of direct participation of thiamine-synthesizing enzymes in hydroxyethylthiazole uptake was discussed.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Yamasaki H,Sanemori H,Yamada K,Kawasaki Tdoi
10.1128/JB.116.3.1280-1286.1973subject
Has Abstractpub_date
1973-12-01 00:00:00pages
1280-6issue
3eissn
0021-9193issn
1098-5530journal_volume
116pub_type
杂志文章abstract::In many organisms, phosphatase expression and phosphate (P) uptake are coordinately regulated by the Pho regulon. In Myxococcus xanthus P limitation initiates multicellular development, a process associated with changes in phosphatase expression. We sought here to characterize the link between P acquisition and develo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01781-07
更新日期:2008-03-01 00:00:00
abstract::The multicellular developmental cycle of Myxococcus xanthus requires large-scale changes in gene transcription, and recent findings indicate that NtrC-like activators play a prominent role in regulating these changes. In this study, we made insertions in 28 uncharacterized ntrC-like activator (nla) genes and found tha...
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journal_title:Journal of bacteriology
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doi:10.1128/JB.98.1.256-263.1969
更新日期:1969-04-01 00:00:00
abstract::A fragment of the Lactococcus lactis chromosome containing an open reading frame of 265 codons, denoted bglR, has been characterized. The polypeptide encoded by bglR shares 36 to 30% sequence identity with a family of regulatory proteins including ArbG from Erwinia chrysanthemi, BglG from Escherichia coli, and SacT an...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.18.5681-5685.1994
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abstract::The extent and nature of DNA polymorphism in the mutS-rpoS region of the Escherichia coli genome were assessed in 21 strains of enteropathogenic E. coli (EPEC) and enterohemorrhagic E. coli (EHEC) and in 6 strains originally isolated from natural populations. The intervening region between mutS and rpoS was amplified ...
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pub_type: 杂志文章
doi:10.1128/JB.181.22.7126-7130.1999
更新日期:1999-11-01 00:00:00
abstract::Analysis of a region involved in the conjugative transfer of the lactococcal conjugative element pRS01 has revealed a bacteria] group II intron. Splicing of this lactococcal intron (designated Ll.ltrB) in vivo resulted in the ligation of two exon messages (ltrBE1 and ltrBE2) which encoded a putative conjugative relaxa...
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doi:10.1128/jb.178.12.3531-3538.1996
更新日期:1996-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:1972-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1989-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.172.7.3620-3630.1990
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更新日期:1997-12-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1990-03-01 00:00:00
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更新日期:2007-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.3.1392-1399.1990
更新日期:1990-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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abstract::A comprehensive equation, upsilon = VM/[1 + (A0.5/Fru-P2)n] [ 1 + (Glc-6-P/I0.5)], has been proposed to represent the quantitative interrelationships between the rate of glucose utilization and the levels of glucose-6-phosphate and fructose-1,6-diphosphate in the intact Escherichia coli cell. This comprehensive equati...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.128.1.165-169.1976
更新日期:1976-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:
更新日期:1982-10-01 00:00:00
abstract:UNLABELLED:Over the course of the last 3 decades the role of the second messenger cyclic di-GMP (c-di-GMP) as a master regulator of bacterial physiology was determined. Although the control over c-di-GMP levels via synthesis and breakdown and the allosteric regulation of c-di-GMP over receptor proteins (effectors) and ...
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更新日期:2008-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:1975-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.6.1971-1977.1991
更新日期:1991-03-01 00:00:00
abstract::The genome sequence of the enteric bacterial pathogen Campylobacter jejuni NCTC 11168 (11168-GS) was published in 2000, providing a valuable resource for the identification of C. jejuni-specific colonization and virulence factors. Surprisingly, the 11168-GS clone was subsequently found to colonize 1-day-old chicks fol...
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更新日期:2004-01-01 00:00:00
abstract::LaBrec, Eugene H., Herman Schneider, Thomas J. Magnani, and Samuel B. Formal. Epithelial cell penetration as an essential step in the pathogenesis of bacillary dysentery. J. Bacteriol. 88:1503-1518. 1964.-A parent strain of Shigella flexneri 2a and a colonial mutant derived from it were studied in three animal models....
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pub_type: 杂志文章
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更新日期:1964-11-01 00:00:00
abstract::We have developed a rapid and sensitive fluorimetric method, based on the formation of a fluorescent product from nitrosation of 2,3-diaminonaphthalene, for measuring the ability of bacteria to catalyze nitrosation of amines. We have shown in Escherichia coli that nitrosation can be induced under anaerobic conditions ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.1.359-364.1988
更新日期:1988-01-01 00:00:00
abstract::A gene coding for thermophilic beta-amylase of Clostridium thermosulfurogenes was cloned into Bacillus subtilis, and its nucleotide sequence was determined. The nucleotide sequence suggested that the thermophilic beta-amylase is translated from monocistronic mRNA as a secretory precursor with a signal peptide of 32 am...
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pub_type: 杂志文章
doi:10.1128/jb.170.12.5848-5854.1988
更新日期:1988-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1992-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1994-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1968-05-01 00:00:00
abstract::In the filamentous, heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120, expression of the nitrate assimilation nirA operon takes place in the absence of ammonium and the presence of nitrate or nitrite. Several positive-action proteins that are required for expression of the nirA operon have been identified...
journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2010-06-01 00:00:00