Bacterial catalysis of nitrosation: involvement of the nar operon of Escherichia coli.

abstract：:A gene encoding only the C-terminal portion of the receptor-transducer protein Tar of Escherichia coli was constructed. The gene product was detected and localized in the cytoplasmic fraction of the cell by immunoblotting with anti-Tar antibodies. The C-terminal fragments from wild-type and mutant tar genes were chara...

journal_title：Journal of bacteriology

authors： Oosawa K,Mutoh N,Simon MI

更新日期：1988-06-01 00:00:00

abstract：:The orientation of lipoteichoic acid (LTA) molecules on the surface of bacterial cells undoubtedly is determined by the ability of the LTA, during its transit through the cell wall, to bind via its polyglycerophosphate backbone or its glycolipid moieties to other constituents of the cytoplasmic membrane and the cell w...

journal_title：Journal of bacteriology

authors： Ofek I,Simpson WA,Beachey EH

更新日期：1982-02-01 00:00:00

abstract：:The Bacillus subtilis sacY and sacT genes encode antiterminator proteins, similar to the Escherichia coli bglG gene product and required for transcription of sucrose metabolism genes. A Tn10 insertion into bglP (formerly sytA) has been previously identified as restoring sucrose utilization to a strain with deletions o...

journal_title：Journal of bacteriology

authors： Le Coq D,Lindner C,Krüger S,Steinmetz M,Stülke J

更新日期：1995-03-01 00:00:00

abstract：:Translational efficiency of an AUG, CUG, GUG, or UUG initiation codon was measured for the naturally leaderless cI mRNA from bacteriophage lambda. In a cI-lacZ translational fusion, only AUG supported a high level of expression; GUG supported a low level of expression, while UUG and CUG expression was barely above bac...

journal_title：Journal of bacteriology

authors： O'Donnell SM,Janssen GR

更新日期：2001-02-01 00:00:00

abstract：:In the course of study on ribonucleic acid (RNA) metabolism during sporulation in Saccharomyces cerevisiae, a new species of RNA (20S) was observed in sporulating cells by polyacrylamide gel electrophoresis. The relative content of this RNA to total RNA increased linearly early in sporulation. Labeled adenine was pref...

journal_title：Journal of bacteriology

authors： Kadowaki K,Halvorson HO

更新日期：1971-03-01 00:00:00

abstract：:During growth of streptomycin-dependent strains of Escherichia coli in the absence of streptomycin (deprived growth), both constitutive and inducible synthesis of beta-galactosidase were preferentially inhibited. A similar preferential inhibition of constitutive and derepressed synthesis of alkaline phosphatase was ob...

journal_title：Journal of bacteriology

authors： Goodman RE,Spotts CR

更新日期：1967-10-01 00:00:00

abstract：:Twelve strains of Agrobacterium radiobacter isolated from naturally occurring crown galls or soil were found to be avirulent on sunflower, tomato, Kalanchoe, and carrot. Eleven strains contained plasmids of molecular weights 77 X 10(6) to 182 X 10(6) as determined by electron microscopy. One strain contained only a sm...

journal_title：Journal of bacteriology

authors： Merlo DJ,Nester EW

更新日期：1977-01-01 00:00:00

abstract：:The porin composition of the Escherichia coli cell envelope was analyzed during growth at different external pHs (pHo) as a function of the acetyl phosphate (AcP) level (DeltaackA pta or ackA mutant, pyruvate or glucose as the carbon source) in the presence or absence of EnvZ. Our results indicate that the AcP level i...

journal_title：Journal of bacteriology

authors： Heyde M,Laloi P,Portalier R

更新日期：2000-01-01 00:00:00

abstract：:Streptococcus agalactiae is a primary cause of neonatal morbidity and mortality. Essential to the virulence of this pathogen is the production of a type-specific capsular polysaccharide (CPS) that enables the bacteria to evade host immune defenses. The identification, cloning, sequencing, and functional characterizati...

journal_title：Journal of bacteriology

authors： Chaffin DO,Beres SB,Yim HH,Rubens CE

更新日期：2000-08-01 00:00:00

abstract：:The chemical properties of the cell wall of Mycobacterium lepraemurium strain Hawaii were investigated. Five subunits of the cell wall, arabinose mycolate, mycolic acids, tetrapeptide (Ala-Gln-diaminopimelic acid-Ala), disaccharide (N-acetylglucosaminyl-beta-1,4-N-glycolylmuramic acid), and arabinogalactan, were obtai...

journal_title：Journal of bacteriology

authors： Azuma I,Yamamura Y,Tanaka Y,Kosaka K,Mori T

更新日期：1973-01-01 00:00:00

abstract：:Unsaturated fatty acids having structural features which are different from those of the monoenoic acids normally synthesized by Escherichia coli can serve as growth factors for an auxotroph requiring unsaturated fatty acids. These analogues were incorporated into the phospholipids, as shown by gas-liquid and thin-lay...

journal_title：Journal of bacteriology

authors： Silbert DF,Ruch F,Vagelos PR

更新日期：1968-05-01 00:00:00

abstract：:Western (immunoblot) analysis using Escherichia coli anti-RecA antiserum revealed that expression of the RecA protein of Pseudomonas aeruginosa PAO is induced upon exposure of the bacterium to UV irradiation or norfloxacin, a quinolone related to nalidixic acid. ...

journal_title：Journal of bacteriology

authors： Miller RV,Kokjohn TA

更新日期：1988-05-01 00:00:00

abstract：:The cell walls of a selected isolate of Staphylococcus aureus FDA 209P were observed undergoing progressive disintegration when exposed to lysostaphin (1 unit/ml) in 24% NaCl solution. Electron micrographs of ultrathin sections of test cells after exposure to lysostaphin for 2 min showed only superficial evidence of l...

journal_title：Journal of bacteriology

authors： Schuhardt VT,Huber TW,Pope LM

更新日期：1969-01-01 00:00:00

abstract：:Gene 1 of bacteriophage T4 has been cloned into a lambda pL expression vector, resulting in the overproduction of deoxynucleotide kinase. A procedure that includes affinity chromatography on Cibacron Blue F3GA-agarose has been used to purify milligram quantities of enzymes from a 2-liter culture. The enzyme has been p...

journal_title：Journal of bacteriology

authors： Brush GS,Bhatnagar SK,Bessman MJ

更新日期：1990-06-01 00:00:00

abstract：:ToxR, the transmembrane regulatory protein required for expression of virulence factors in the human diarrheal pathogen Vibrio cholerae, directly activates and represses the transcription of two outer membrane porins, OmpU and OmpT, respectively. In an attempt to dissect the role of the OmpU and OmpT porins in viabili...

journal_title：Journal of bacteriology

authors： Provenzano D,Lauriano CM,Klose KE

更新日期：2001-06-01 00:00:00

abstract：:The D1:1 protein and its potentially occurring degradation products were overexpressed in Escherichia coli. Protein-DNA interaction is shown for the promoter region of psbAI. The D1:1 degradation products may be involved in transcription regulation of psbAI by binding in the promoter region. Additionally, C-terminal f...

journal_title：Journal of bacteriology

authors： Stelljes C,Koenig F

更新日期：2007-03-01 00:00:00

abstract：:A monoclonal antibody, AFRC MAC 203, was used to examine the expression of a nodule-induced cell surface antigen associated with lipopolysaccharide in Rhizobium leguminosarum bv. viciae 3841. Silver-enhanced immunogold-labeled tissue sections revealed that, in very young tissues of pea root nodules, the nodule-induced...

journal_title：Journal of bacteriology

authors： VandenBosch KA,Brewin NJ,Kannenberg EL

更新日期：1989-09-01 00:00:00

abstract：:Bacillus brevis HPD31 contains a surface (S)-layer protein, termed the HWP, which forms a hexagonal array in the cell wall. The 5' region of the HWP gene was isolated from a DNA library constructed in bacteriophage vector EMBL3 from a partial BamHI digest of the chromosomal DNA. The 3' region contained in a 2.7-kiloba...

journal_title：Journal of bacteriology

authors： Ebisu S,Tsuboi A,Takagi H,Naruse Y,Yamagata H,Tsukagoshi N,Udaka S

更新日期：1990-03-01 00:00:00

abstract：:Smith, Rodney F. (University of Maryland, Baltimore), Donald E. Shay, and Norman J. Doorenbos. Antimicrobial action of nitrogen-containing steroids. J. Bacteriol. 85:1295-1299. 1963.-A new group of 16 synthetic nitrogen-containing steroids have been tested against a variety of microorganisms for antimicrobial properti...

journal_title：Journal of bacteriology

authors： SMITH RF,SHAY DE,DOORENBOS NJ

更新日期：1963-06-01 00:00:00

abstract：:Nearly every known species of Eubacteria encodes a homolog of the Borrelia burgdorferi EbfC DNA-binding protein. We now demonstrate that fluorescently tagged EbfC associates with B. burgdorferi nucleoids in vivo and that chromatin immunoprecipitation (ChIP) of wild-type EbfC showed it to bind in vivo to sites througho...

journal_title：Journal of bacteriology

authors： Jutras BL,Bowman A,Brissette CA,Adams CA,Verma A,Chenail AM,Stevenson B

更新日期：2012-07-01 00:00:00

abstract：:The Neurospora crassa assimilatory nitrite reductase structural gene, nit-6, has been isolated. A cDNA library was constructed from poly(A)+ RNA isolated from Neurospora mycelia in which nitrate assimilation had been induced. This cDNA was ligated into lambda ZAP II (Stratagene) and amplified. This library was then sc...

journal_title：Journal of bacteriology

authors： Exley GE,Colandene JD,Garrett RH

更新日期：1993-04-01 00:00:00

abstract：:Freeze-etching was applied to preparations, with and without glycerol, of Acinetobacter sp. strain MJT/F5/199A, consisting of intact cells after normal growth or after incubation with chloramphenicol, spheroplasts, and isolated cell walls and outer membranes. Etched preparations show that a regular array of subunits f...

journal_title：Journal of bacteriology

authors： Sleytr UB,Thornley MJ

更新日期：1973-12-01 00:00:00

abstract：:Escherichia coli MutY and MutS increase replication fidelity by removing adenines that were misincorporated opposite 7,8-dihydro-8-oxo-deoxyguanines (8-oxoG), G, or C. MutY DNA glycosylase removes adenines from these mismatches through a short-patch base excision repair pathway and thus prevents G:C-to-T:A and A:T-to-...

journal_title：Journal of bacteriology

authors： Bai H,Lu AL

更新日期：2007-02-01 00:00:00

abstract：:In this report we describe the functional expression of EmrE, a 110-amino-acid multidrug transporter from Escherichia coli, in the yeast Saccharomyces cerevisiae. To allow for phenotypic complementation, a mutant strain sensitive to a series of cationic lipophilic drugs was first identified. A hemagglutinin epitope-ta...

journal_title：Journal of bacteriology

authors： Yelin R,Rotem D,Schuldiner S

更新日期：1999-02-01 00:00:00

abstract：:The yeast two-hybrid assay revealed that Bacillus subtilis DnaD, a possible component of the primosome and required for replication initiation, interacted with DnaA and DnaD itself. The mutant DnaD23 was incapable of interacting with DnaA but retained interaction with the wild-type DnaD. These results suggest that int...

journal_title：Journal of bacteriology

authors： Ishigo-Oka D,Ogasawara N,Moriya S

更新日期：2001-03-01 00:00:00

abstract：:Formation of the Escherichia coli division septum is catalyzed by a number of essential proteins (named Fts) that assemble into a ring-like structure at the future division site. Many of these Fts proteins are intrinsic transmembrane proteins whose functions are largely unknown. In the present study, we attempted to i...

journal_title：Journal of bacteriology

authors： Karimova G,Robichon C,Ladant D

更新日期：2009-01-01 00:00:00

abstract：:Two temperature-sensitive sporulation mutants have been characterized. One mutant, which is blocked at stage II, has a short temperature-sensitive period that occurs at about the time when the spore septum is formed. Cells can escape the sporulation block, if incubated for a short period at the permissive temperature,...

journal_title：Journal of bacteriology

authors： Young M

更新日期：1976-05-01 00:00:00

abstract：:A selection procedure is described which permits a large number of Saccharomyces cerevisiae mutants to be screened for specific lesions in mitochondrial respiratory enzymes and the adenosine triphosphatase. The method has been used to isolate nuclear mutant strains with specific lesions in coenzyme QH2-cytochrome c re...

journal_title：Journal of bacteriology

authors： Tzagoloff A,Akai A,Needleman RB

更新日期：1975-06-01 00:00:00

abstract：:Pseudomonas aeruginosa is an opportunistic bacterial pathogen which poses a major threat to long-term-hospitalized patients and individuals with cystic fibrosis. The capacity of P. aeruginosa to form biofilms is an important requirement for chronic colonization of human tissues and for persistence in implanted medical...

journal_title：Journal of bacteriology

authors： Vallet I,Diggle SP,Stacey RE,Cámara M,Ventre I,Lory S,Lazdunski A,Williams P,Filloux A

更新日期：2004-05-01 00:00:00

abstract：:Natural and immune human antibodies reactive with heat-labile and heat-stable antigens of virulent Neisseria gonorrhoeae were studied by use of an indirect fluorescent-antibody (IFA) procedure. The immunoglobulin class of the reactive antibodies was identified by using fluorescein-conjugated antisera specific for huma...

journal_title：Journal of bacteriology

authors： Cohen IR

更新日期：1967-07-01 00:00:00