Abstract:
:Pseudomonas aeruginosa is an opportunistic bacterial pathogen which poses a major threat to long-term-hospitalized patients and individuals with cystic fibrosis. The capacity of P. aeruginosa to form biofilms is an important requirement for chronic colonization of human tissues and for persistence in implanted medical devices. Various stages of biofilm formation by this organism are mediated by extracellular appendages, such as type IV pili and flagella. Recently, we identified three P. aeruginosa gene clusters that were termed cup (chaperone-usher pathway) based on their sequence relatedness to the chaperone-usher fimbrial assembly pathway in other bacteria. The cupA gene cluster, but not the cupB or cupC cluster, is required for biofilm formation on abiotic surfaces. In this study, we identified a gene (mvaT) encoding a negative regulator of cupA expression. Such regulatory control was confirmed by several approaches, including lacZ transcriptional fusions, Northern blotting, and transcriptional profiling using DNA microarrays. MvaT also represses the expression of the cupB and cupC genes, although the extent of the regulatory effect is not as pronounced as with cupA. Consistent with this finding, mvaT mutants exhibit enhanced biofilm formation. Although the P. aeruginosa genome contains a highly homologous gene, mvaU, the repression of cupA genes is MvaT specific. Thus, MvaT appears to be an important regulatory component within a complex network that controls biofilm formation and maturation in P. aeruginosa.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Vallet I,Diggle SP,Stacey RE,Cámara M,Ventre I,Lory S,Lazdunski A,Williams P,Filloux Adoi
10.1128/jb.186.9.2880-2890.2004keywords:
subject
Has Abstractpub_date
2004-05-01 00:00:00pages
2880-90issue
9eissn
0021-9193issn
1098-5530journal_volume
186pub_type
杂志文章abstract::Low-copy-number plasmids generally encode a partitioning system to ensure proper segregation after replication. Little is known about partitioning of linear plasmids in Streptomyces. SLP2 is a 50-kb low-copy-number linear plasmid in Streptomyces lividans, which contains a typical parAB partitioning operon. In S. livid...
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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doi:10.1128/jb.174.17.5676-5685.1992
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journal_title:Journal of bacteriology
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更新日期:1993-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:1975-09-01 00:00:00
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journal_title:Journal of bacteriology
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pub_type: 杂志文章
doi:10.1128/JB.96.4.1029-1036.1968
更新日期:1968-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.16.5010-5016.1991
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journal_title:Journal of bacteriology
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doi:10.1128/jb.177.22.6610-6618.1995
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.4.1060-1064.2004
更新日期:2004-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.4.1440-1445.1966
更新日期:1966-04-01 00:00:00
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doi:10.1128/JB.140.2.567-573.1979
更新日期:1979-11-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/JB.159.1.306-311.1984
更新日期:1984-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:1970-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.1.358-364.1969
更新日期:1969-10-01 00:00:00
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更新日期:1983-10-01 00:00:00
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更新日期:1971-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2006-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.2.435-443.1976
更新日期:1976-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.15.4936-4940.1993
更新日期:1993-08-01 00:00:00