Abstract:
:Escherichia coli MutY and MutS increase replication fidelity by removing adenines that were misincorporated opposite 7,8-dihydro-8-oxo-deoxyguanines (8-oxoG), G, or C. MutY DNA glycosylase removes adenines from these mismatches through a short-patch base excision repair pathway and thus prevents G:C-to-T:A and A:T-to-G:C mutations. MutS binds to the mismatches and initiates the long-patch mismatch repair on daughter DNA strands. We have previously reported that the human MutY homolog (hMYH) physically and functionally interacts with the human MutS homolog, hMutSalpha (Y. Gu et al., J. Biol. Chem. 277:11135-11142, 2002). Here, we show that a similar relationship between MutY and MutS exists in E. coli. The interaction of MutY and MutS involves the Fe-S domain of MutY and the ATPase domain of MutS. MutS, in eightfold molar excess over MutY, can enhance the binding activity of MutY with an A/8-oxoG mismatch by eightfold. The MutY expression level and activity in mutS mutant strains are sixfold and twofold greater, respectively, than those for the wild-type cells. The frequency of A:T-to-G:C mutations is reduced by two- to threefold in a mutS mutY mutant compared to a mutS mutant. Our results suggest that MutY base excision repair and mismatch repair defend against the mutagenic effect of 8-oxoG lesions in a cooperative manner.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Bai H,Lu ALdoi
10.1128/JB.01513-06subject
Has Abstractpub_date
2007-02-01 00:00:00pages
902-10issue
3eissn
0021-9193issn
1098-5530pii
JB.01513-06journal_volume
189pub_type
杂志文章abstract::Developmental expression of the Myxococcus xanthus gene 4521 requires extracellular A-signal. This signal is generated in response to nutrient limitation and functions in cell density sensing. To identify the upstream limit of the minimum region required in vivo for A-signal-dependent 4521 expression, a 5' deletion an...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.16.4645-4651.1995
更新日期:1995-08-01 00:00:00
abstract::The four methyl-accepting chemotaxis proteins of Escherichia coli, often called transducers, are transmembrane receptor proteins that exhibit substantial identity among the sequences of their cytoplasmic domains. Thus, antiserum raised to one of these proteins recognizes the others and might be expected to recognize r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.1.133-140.1993
更新日期:1993-01-01 00:00:00
abstract::The neuS-encoded polysialytransferase (polyST) in Escherichia coli K1 catalyzes synthesis of polysialic acid homopolymers composed of unbranched sialyl alpha 2,8 linkages. Subcloning and complementation experiments showed that the K1 neuS was functionally interchangeable with the neuS from E. coli K92 (S. M. Steenberg...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.15.5127-5131.1992
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abstract::Genome sequencing revealed that all six chlamydiae genomes contain three groEL-like genes (groEL1, groEL2, and groEL3). Phylogenetic analysis of groEL1, groEL2, and groEL3 indicates that these genes are likely to have been present in chlamydiae since the beginning of the lineage. Comparison of deduced amino acid seque...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.6.1958-1966.2003
更新日期:2003-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.16.5392-5399.2004
更新日期:2004-08-01 00:00:00
abstract::Mycoplasma mobile has a unique mechanism that enables it to glide on solid surfaces faster than any other gliding mycoplasma. To elucidate the gliding mechanism, we developed a transformation system for M. mobile based on a transposon derived from Tn4001. Modification of the electroporation conditions, outgrowth time,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01418-13
更新日期:2014-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.166.2.574-580.1986
更新日期:1986-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.6.1971-1974.1999
更新日期:1999-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.21.6262-6268.2003
更新日期:2003-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.151.3.1153-1161.1982
更新日期:1982-09-01 00:00:00
abstract::Sphingomonas wittichii RW1 degrades chlorinated dibenzofurans and dibenzo-p-dioxins via meta cleavage. We used inverse PCR to amplify dxnB2, a gene encoding one of three meta-cleavage product (MCP) hydrolases identified in the organism that are homologues of BphD involved in biphenyl catabolism. Purified DxnB2 catalyz...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01950-06
更新日期:2007-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.121.1.234-238.1975
更新日期:1975-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.1.231-239.1969
更新日期:1969-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.17.5598-5601.1997
更新日期:1997-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.12.5680-5688.1988
更新日期:1988-12-01 00:00:00
abstract::Cerulenin is a fungal mycotoxin that potently inhibits fatty acid synthesis by covalent modification of the active site thiol of the chain-elongation subtypes of beta-ketoacyl-acyl carrier protein (ACP) synthases. The Bacillus subtilis fabF (yjaY) gene (fabF(b)) encodes an enzyme that catalyzes the condensation of mal...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.10.3032-3040.2001
更新日期:2001-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.127.1.536-544.1976
更新日期:1976-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.133.1.390-391.1978
更新日期:1978-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2008-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01949-06
更新日期:2007-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.12.3400-3404.2000
更新日期:2000-06-01 00:00:00
abstract::Lectin activity in a host-mycoparasite relationship was demonstrated with Rhizoctonia solani and Trichoderma harzianum. Attachment of O but not A and B erythrocytes to hyphae occurred on R. solani but not on its mycoparasite. This phenomenon, which was Ca2+ and Mn2+ dependent, was prevented by galactose, present in T....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.154.3.1431-1435.1983
更新日期:1983-06-01 00:00:00
abstract::The dnaA operon of Escherichia coli contains the genes dnaA, dnaN, and recF encoding DnaA, beta clamp of DNA polymerase III holoenzyme, and RecF. When the DnaA concentration is raised, an increase in the number of DNA replication initiation events but a reduction in replication fork velocity occurs. Because DnaA is au...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.2.630-644.2003
更新日期:2003-01-01 00:00:00
abstract::Bacillus subtilis OhrR is the prototype for the one-Cys family of organic peroxide-sensing regulatory proteins. Mutational analyses indicate that the high sensitivity of the active site cysteine (C15) to peroxidation requires three Tyr residues. Y29 and Y40 from the opposing subunit of the functional dimer hydrogen bo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00879-07
更新日期:2007-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.18.5699-5704.1991
更新日期:1991-09-01 00:00:00
abstract::Nocardia brasiliensis possess proteolytic activities that can be readily detected in a variety of media. In a modified formulation of a growth medium originally used for Streptomyces aureofaciens, N. brasiliensis was found to secrete proteolytic enzymes, one of which was capable of hydrolyzing casein. This enzyme was ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.157.2.627-631.1984
更新日期:1984-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.10.4692-4695.1987
更新日期:1987-10-01 00:00:00
abstract::Vi-agglutinin, active cutaneous anaphylaxis and protective responses (ed(50)) of three mouse strains (CFW, NIH, and Balb/cAnN) to acetone-inactivated typhoid vaccine and soluble Vi antigen were compared. Seven days after immunization with either typhoid vaccine or Vi antigen the three strains of mice differed with res...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.99.1.8-12.1969
更新日期:1969-07-01 00:00:00
abstract::The Escherichia coli promoter p(BAD), under the control of the AraC protein, drives the expression of mRNA encoding the AraB, AraA, and AraD gene products of the arabinose operon. The binding site of AraC at p(BAD) overlaps the RNA polymerase -35 recognition region by 4 bases, leaving 2 bases of the region not contact...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.18.5076-5081.2000
更新日期:2000-09-01 00:00:00
abstract::Riemerella anatipestifer is responsible for exudative septicemia in ducks. The genetic determinant of the CAMP cohemolysin, cam, from a strain of R. anatipestifer was cloned and expressed in Escherichia coli. Chromosomal DNA from serotype 19 strain 30/90 was used to construct a gene library in pBluescript II SK(-) vec...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.7.1932-1939.2002
更新日期:2002-04-01 00:00:00