Cloning and expression of the exfoliative toxin B gene from Staphylococcus aureus.

abstract：:Coxiella burnetii, the etiological agent of acute and chronic Q fever in humans, is a naturally intracellular pathogen that directs the formation of an acidic Coxiella-containing vacuole (CCV) derived from the host lysosomal network. Central to its pathogenesis is a specialized type IVB secretion system (T4SS) that de...

journal_title：Journal of bacteriology

authors： Weber MM,Chen C,Rowin K,Mertens K,Galvan G,Zhi H,Dealing CM,Roman VA,Banga S,Tan Y,Luo ZQ,Samuel JE

更新日期：2013-09-01 00:00:00

abstract：:Phospholipid synthesis has been reported to be subject to stringent control in Escherichia coli. We present evidence that demonstrates a strict correlation between guanosine tetraphosphate accumulation and inhibition of phospholipid synthesis. In vivo experiments designed to examine the pattern of phospholipid labelin...

journal_title：Journal of bacteriology

authors： Merlie JP,Pizer LI

更新日期：1973-10-01 00:00:00

abstract：:Englesberg, E. (University of Pittsburgh, Pittsburgh, Pa.), R L. Anderson, R. Weinberg, N. Lee, P. Hoffee, G. Huttenhauer, and H. Boyer. l-Arabinose-sensitive, l-ribulose 5-phosphate 4-epimerase-deficient mutants of Escherichia coli. J. Bacteriol. 84:137-146. 1962-l-Arabinose-negative mutants of Escherichia coli B/r, ...

journal_title：Journal of bacteriology

authors： ENGLESBERG E,ANDERSON RL,WEINBERG R,LEE N,HOFFEE P,HUTTENHAUER G,BOYER H

更新日期：1962-07-01 00:00:00

abstract：:Despite their remarkable genetic homology, members of the Mycobacterium tuberculosis complex express very different phenotypes, most notably in their spectra of clinical presentation. For example, M. tuberculosis is regarded as pathogenic to humans, whereas members having deleted RD1, such as Mycobacterium microti and...

journal_title：Journal of bacteriology

authors： Mostowy S,Cousins D,Behr MA

更新日期：2004-01-01 00:00:00

abstract：:The sheath of the filamentous, gliding cyanobacterium Phormidium uncinatum was studied by using light and electron microscopy. In thin sections and freeze fractures the sheath was found to be composed of helically arranged carbohydrate fibrils, 4 to 7 nm in diameter, which showed a substantial degree of crystallinity....

journal_title：Journal of bacteriology

authors： Hoiczyk E

更新日期：1998-08-01 00:00:00

abstract：UNLABELLED:Bacterial type IV coupling proteins (T4CPs) bind and mediate the delivery of DNA substrates through associated type IV secretion systems (T4SSs). T4CPs consist of a transmembrane domain, a conserved nucleotide-binding domain (NBD), and a sequence-variable helical bundle called the all-alpha domain (AAD). In ...

journal_title：Journal of bacteriology

authors： Whitaker N,Chen Y,Jakubowski SJ,Sarkar MK,Li F,Christie PJ

更新日期：2015-07-01 00:00:00

abstract：:Vibrio parahaemolyticus possesses dual flagellar systems adapted for movement under different circumstances. A single polar flagellum propels the bacterium in liquid (i.e., swimming) with a motor that is powered by the sodium motive force. Multiple proton-driven lateral flagella enable translocation over surfaces (i.e...

journal_title：Journal of bacteriology

authors： Stewart BJ,McCarter LL

更新日期：2003-08-01 00:00:00

abstract：:Romano, Antonio H. (University of Cincinnati, Cincinnati, Ohio), and Dorothy J. Geason. Pattern of sheath synthesis in Sphaerotilus natans. J. Bacteriol. 88:1145-1150. 1964.-Formation of the characteristic sheath of Sphaerotilus natans was followed by immunofluorescence. Fluorescent antisheath antibody was obtained by...

journal_title：Journal of bacteriology

authors： ROMANO AH,GEASON DJ

更新日期：1964-10-01 00:00:00

abstract：:Four rpoD hybridizing signals have been identified in the chromosome of Brevibacterium lactofermentum. Two rpoD-like genes, sigA and sigB, have been cloned and sequenced, and they encode principal sigma factors of the RNA polymerase. The deduced amino acid sequences of SigA and SigB showed very high similarities to th...

journal_title：Journal of bacteriology

authors： Oguiza JA,Marcos AT,Malumbres M,Martín JF

更新日期：1996-01-01 00:00:00

abstract：:We have identified a gene encoding a putative membrane protein homologous to the major facilitator superfamily, mapping upstream of the lacS gene in Sulfolobus solfataricus. Permeases from this family mediate secondary transport and are widely distributed among eubacteria and eukaryotes; the finding of an archaeal mem...

journal_title：Journal of bacteriology

authors： Prisco A,Moracci M,Rossi M,Ciaramella M

更新日期：1995-03-01 00:00:00

abstract：:In order to examine the potential role of bacterial collagenases in periodontal tissue destruction, we recently isolated a gene, prtC, from Porphyromonas gingivalis ATCC 53977, which expressed collagenase activity (N. Takahashi, T. Kato, and H. K. Kuramitsu, FEMS Microbiol. Lett. 84:135-138, 1991). The nucleotide sequ...

journal_title：Journal of bacteriology

authors： Kato T,Takahashi N,Kuramitsu HK

更新日期：1992-06-01 00:00:00

abstract：:Pathogenic yersiniae require Ca2+ for growth at 37 degrees C. They harbor closely related plasmids of about 70 kb that are essential for virulence. At 37 degrees C and in the absence of Ca2+ ions, these plasmids cause a decrease in growth rate and the release of large amounts of proteins called Yops. Here we describe ...

journal_title：Journal of bacteriology

authors： Allaoui A,Scheen R,Lambert de Rouvroit C,Cornelis GR

更新日期：1995-08-01 00:00:00

abstract：:Sixteen species of enterobacteria have been screened for mutagenic DNA repair activity. In Escherichia coli, mutagenic DNA repair is encoded by the umuDC operon. Synthesis of UmuD and UmuC proteins is induced as part of the SOS response to DNA damage, and after induction, the UmuD protein undergoes an autocatalytic cl...

journal_title：Journal of bacteriology

authors： Sedgwick SG,Ho C,Woodgate R

更新日期：1991-09-01 00:00:00

abstract：:Two sets of genes for the large and small subunits of ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO) were detected in the photosynthetic purple sulfur bacterium Chromatium vinosum by hybridization analysis with RuBisCO gene probes, cloned by using the lambda Fix vector, and designated rbcL-rbcS and rbcA-rbc...

journal_title：Journal of bacteriology

authors： Viale AM,Kobayashi H,Akazawa T

更新日期：1989-05-01 00:00:00

abstract：:Proteins induced by acid or base, during long-term aerobic or anaerobic growth in complex medium, were identified in Escherichia coli. Two-dimensional gel electrophoresis revealed pH-dependent induction of 18 proteins, nine of which were identified by N-terminal sequencing. At pH 9, tryptophan deaminase (TnaA) was ind...

journal_title：Journal of bacteriology

authors： Blankenhorn D,Phillips J,Slonczewski JL

更新日期：1999-04-01 00:00:00

abstract：:Shewanella oneidensis MR-1 is a facultatively anaerobic bacterium capable of using soluble and insoluble forms of manganese [Mn(III/IV)] and iron [Fe(III)] as terminal electron acceptors during anaerobic respiration. To assess the structural association of two outer membrane-associated c-type decaheme cytochromes (i.e...

journal_title：Journal of bacteriology

authors： Shi L,Chen B,Wang Z,Elias DA,Mayer MU,Gorby YA,Ni S,Lower BH,Kennedy DW,Wunschel DS,Mottaz HM,Marshall MJ,Hill EA,Beliaev AS,Zachara JM,Fredrickson JK,Squier TC

更新日期：2006-07-01 00:00:00

abstract：:The kil-kor regulon of the self-transmissible, broad-host-range plasmid RK2 is a unique network with eight coregulated operons. Among the genes encoded by the kil-kor regulon are trfA, which encodes the replication initiator, and several kil loci (kilA, kilB, kilC, and kilE), each of which is lethal to the host cell i...

journal_title：Journal of bacteriology

authors： Thomson VJ,Jovanovic OS,Pohlman RF,Chang CH,Figurski DH

更新日期：1993-04-01 00:00:00

abstract：:We purified active hydrogenase from free-living Rhizobium japonicum by affinity chromatography. The uptake hydrogenase of R. japonicum has been treated previously as an oxygen-sensitive protein. In this purification, however, reducing agents were not added nor was there any attempt to exclude oxygen. In fact, the addi...

journal_title：Journal of bacteriology

authors： Stults LW,Moshiri F,Maier RJ

更新日期：1986-06-01 00:00:00

abstract：:Extracytoplasmic function σ factors that are stress inducible are often sequestered in an inactive complex with a membrane-associated anti-σ factor. Mycobacterium tuberculosis membrane-associated anti-σ factors have a small, stable RNA gene A (ssrA)-like degron for targeted proteolysis. Interaction between the unfolda...

journal_title：Journal of bacteriology

authors： Joshi AC,Kaur P,Nair RK,Lele DS,Nandicoori VK,Gopal B

更新日期：2019-02-25 00:00:00

abstract：:Bulen, William A. (Charles F. Kettering Foundation, Yellow Springs, Ohio). Effect of tungstate on the uptake and function of molybdate in Azotobacter agilis. J. Bacteriol. 82:130-134. 1961.-The reported competitive inhibition of molybdate by tungstate was investigated in an effort to elucidate molybdenum functions ass...

journal_title：Journal of bacteriology

authors： Bulen WA

更新日期：1961-07-01 00:00:00

abstract：:In Streptococcus mutans, competence for genetic transformation and biofilm formation are dependent on the two-component signal transduction system ComDE together with the inducer peptide pheromone competence-stimulating peptide (CSP) (encoded by comC). Here, it is shown that the same system is also required for expres...

journal_title：Journal of bacteriology

authors： van der Ploeg JR

更新日期：2005-06-01 00:00:00

abstract：:wbdA is a mannosyltransferase gene that is involved in synthesis of the Escherichia coli O9a polysaccharide, a mannose homopolymer with a repeating unit of 2-alphaMan-1,2-alphaMan-1,3-alphaMan-1, 3-alphaMan-1. The equivalent structural O polysaccharide in the E. coli O9 and Klebsiella O3 strains is 2-alphaMan-1,2-alph...

journal_title：Journal of bacteriology

authors： Kido N,Kobayashi H

更新日期：2000-05-01 00:00:00

abstract：:Short-term labeling with radioactive thymidine led to inaccurate estimates of deoxyribonucleic acid synthesis at some growth rates in steady-state cultures of Escherichia coli B/r. Estimates were corrected in chemostat cultures by adding adenosine, a known inhibitor of thymidine phosphorylase. ...

journal_title：Journal of bacteriology

authors： Kubitschek HE,Valdez D,Freedman ML

更新日期：1972-06-01 00:00:00

abstract：:Salmonella typhimurium invasion of nonphagocytic cells requires the expression of a type III secretion system (TTSS) encoded within Salmonella pathogenicity island 1 (SPI1). TTSS gene transcription is activated in response to environmental signals and requires transcriptional regulators encoded within (HilA) and outsi...

journal_title：Journal of bacteriology

authors： Rakeman JL,Bonifield HR,Miller SI

更新日期：1999-05-01 00:00:00

abstract：:We investigated the capacity of the hyperthermophile Pyrococcus furiosus for DNA repair by measuring survival at high levels of 60Co gamma-irradiation. The P. furiosus 2-Mb chromosome was fragmented into pieces ranging from 500 kb to shorter than 30 kb at a dose of 2,500 Gy and was fully restored upon incubation at 95...

journal_title：Journal of bacteriology

authors： DiRuggiero J,Santangelo N,Nackerdien Z,Ravel J,Robb FT

更新日期：1997-07-01 00:00:00

abstract：:The Zymomonas mobilis gene (sacA) encoding a protein with sucrase activity has been cloned in Escherichia coli and its nucleotide sequence has been determined. Potential ribosome-binding site and promoter sequences were identified in the region upstream of the gene which were homologous to E. coli and Z. mobilis conse...

journal_title：Journal of bacteriology

authors： Gunasekaran P,Karunakaran T,Cami B,Mukundan AG,Preziosi L,Baratti J

更新日期：1990-12-01 00:00:00

abstract：:It previously has been proposed that the Escherichia coli SecB protein promotes the export of the maltose-binding protein (MBP) from the cytoplasm by preventing the folding of the precursor MBP (preMBP) into a translocation-incompetent conformation. The export of wild-type MBP is only partially blocked in SecB- cells....

journal_title：Journal of bacteriology

authors： Collier DN,Bassford PJ Jr

更新日期：1989-09-01 00:00:00

abstract：:Bacillus subtilis JH642 and a wild strain of B. subtilis called 22a both produce an antilisterial peptide that can be purified by anion-exchange and gel filtration chromatography. Amino acid analysis confirmed that the substance was the cyclic bacteriocin subtilosin. A mutant defective in production of the substance w...

journal_title：Journal of bacteriology

authors： Zheng G,Yan LZ,Vederas JC,Zuber P

更新日期：1999-12-01 00:00:00

abstract：:Gill lamellae from the young fruiting bodies of the basidiomycete Coprinus micaceus possess cytoplasmic particles with cytochemically demonstrable acid phosphatase activity; they are presumed to be lysosomes. ...

journal_title：Journal of bacteriology

authors： Nehemiah JL

更新日期：1973-07-01 00:00:00

abstract：:Construction and characterization of a class of multicopy plasmid cloning vehicles containing the replication system of miniplasmid P15A are described. The constructed plasmids have cleavage sites within antibiotic resistance genes for a variety of commonly employed site-specific endonucleases, permitting convenient u...

journal_title：Journal of bacteriology

authors： Chang AC,Cohen SN

更新日期：1978-06-01 00:00:00