Abstract:
:We investigated the capacity of the hyperthermophile Pyrococcus furiosus for DNA repair by measuring survival at high levels of 60Co gamma-irradiation. The P. furiosus 2-Mb chromosome was fragmented into pieces ranging from 500 kb to shorter than 30 kb at a dose of 2,500 Gy and was fully restored upon incubation at 95 degrees C. We suggest that recombination repair could be an extremely active repair mechanism in P. furiosus and that it might be an important determinant of survival of hyperthermophiles at high temperatures.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
DiRuggiero J,Santangelo N,Nackerdien Z,Ravel J,Robb FTdoi
10.1128/jb.179.14.4643-4645.1997subject
Has Abstractpub_date
1997-07-01 00:00:00pages
4643-5issue
14eissn
0021-9193issn
1098-5530journal_volume
179pub_type
杂志文章abstract::In Escherichia coli, synthesis of sialic acid is not regulated by allosteric inhibition mediated by cytidine 5'-monophospho-N-acetylneuraminic acid (CMP-NeuNAc). Evidence for the lack of metabolic control by feedback inhibition was demonstrated by measuring the intracellular level of sialic acid and CMP-NeuNAc in muta...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.164.2.854-860.1985
更新日期:1985-11-01 00:00:00
abstract::We previously described a bacteriophage of the Lyme disease agent Borrelia burgdorferi designated phiBB-1. This phage packages the host complement of the 32-kb circular plasmids (cp32s), a group of homologous molecules found throughout the genus Borrelia. To demonstrate the ability of phiBB-1 to package and transduce ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.16.4771-4778.2001
更新日期:2001-08-01 00:00:00
abstract::Human dental biofilm communities comprise several species, which can interact cooperatively or competitively. Bacterial interactions influence biofilm formation, metabolic changes, and physiological function of the community. Lactic acid, a common metabolite of oral bacteria, was measured in the flow cell effluent of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01631-09
更新日期:2010-06-01 00:00:00
abstract::Livers of uninfected guinea pigs and of guinea pigs infected with Coxiella burneti were fractionated into smooth endoplasmic reticulum, rough endoplasmic reticulum (RER), pellet, and cell sap fractions. The ribonucleic acid (RNA) and protein of each fraction were determined, and the phosphorylase, glucose-6-phosphatas...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.102.2.334-340.1970
更新日期:1970-05-01 00:00:00
abstract::Dehalogenases are key enzymes in the metabolism of halo-organic compounds. This paper describes a systematic approach to the isolation and molecular analysis of two families of bacterial alpha-halocarboxylic acid (alphaHA) dehalogenase genes, called group I and group II deh genes. The two families are evolutionarily u...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.8.2535-2547.1999
更新日期:1999-04-01 00:00:00
abstract::Neisseria gonorrhoeae (gonococci) and Neisseria meningitidis (meningococci) are human pathogens that cause gonorrhea and meningococcal meningitis, respectively. Both N. gonorrhoeae and N. meningitidis release a number of small peptidoglycan (PG) fragments, including proinflammatory PG monomers, although N. meningitidi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00437-16
更新日期:2016-10-07 00:00:00
abstract::Membrane vesicles containing internal alcohol dehydrogenase and nicotinamide adenine dinucleotide (NAD) were prepared from Escherichia coli ML308-225. Upon the addition of ethanol, these vesicles respired and transported several amino acids. Transport rates driven by internally generated reduced NAD (NADH) were about ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.120.2.861-865.1974
更新日期:1974-11-01 00:00:00
abstract::McFadden, B. A. (Washington State University, Pullman), and H. R. Homann. Characteristics and intermediates of short-term C(14)O(2) incorporation during ribose oxidation by Hydrogenomonas facilis. J. Bacteriol. 89:839-847. 1965.-Ribose-grown cells of Hydrogenomonas facilis, which had been suspended in growth medium an...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.3.839-847.1965
更新日期:1965-03-01 00:00:00
abstract::Activation of the late prespore-specific RNA polymerase sigma factor sigma(G) during Bacillus subtilis sporulation coincides with completion of the engulfment process, when the prespore becomes a protoplast fully surrounded by the mother cell cytoplasm and separated from it by a double membrane system. Activation of s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00715-08
更新日期:2008-12-01 00:00:00
abstract::The ToxR protein of Vibrio cholerae is an integral membrane protein that coordinately regulates the expression of virulence genes required for successful infection. ToxR has been shown to bind directly to and activate transcription of the cholera toxin (ctx) promoter. Within the amino-terminal cytoplasmic region of To...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.21.6807-6814.1992
更新日期:1992-11-01 00:00:00
abstract::The fim genetic switch in the chromosome of Escherichia coli K-12 is an invertible DNA element that harbors the promoter for transcription of the downstream fim structural genes and a transcription terminator that acts on the upstream fimE regulatory gene. Switches oriented appropriately for structural gene transcript...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.24.8256-8266.2005
更新日期:2005-12-01 00:00:00
abstract::The prevalence of the Moraxella catarrhalis immunoglobulin D (IgD)-binding outer membrane protein MID and its gene was determined in 91 clinical isolates and in 7 culture collection strains. Eighty-four percent of the clinical Moraxella strains expressed MID-dependent IgD binding. The mid gene was detected in all stra...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.7.2285-2295.2003
更新日期:2003-04-01 00:00:00
abstract::Numerous field isolates of Moraxella bovis have previously been classified by serological techniques into seven serogroups, each defined by homologous cross-reaction with antisera prepared against purified pili of a single prototype strain. The gene encoding pilin from each of the prototype strains has been characteri...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.16.4875-4882.1994
更新日期:1994-08-01 00:00:00
abstract::A pLAFR3 cosmid clone designated pVir2 containing a 25-kilobase (kb) DNA insert was isolated from a wild-type Pseudomonas solanacearum GMI1000 genomic library. This cosmid was shown to complement all but one of the nine Tn5-induced mutants which have been isolated after random mutagenesis and which have lost both path...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.12.5626-5632.1987
更新日期:1987-12-01 00:00:00
abstract::We have found, using a newly developed genetic method, a protein (named Cnu, for oriC-binding nucleoid-associated) that binds to a specific 26-base-pair sequence (named cnb) in the origin of replication of Escherichia coli, oriC. Cnu is composed of 71 amino acids (8.4 kDa) and shows extensive amino acid identity to a ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.20.6998-7008.2005
更新日期:2005-10-01 00:00:00
abstract::CTXphi is a filamentous bacteriophage that encodes cholera toxin. CTXphi infection of its host bacterium, Vibrio cholerae, requires the toxin-coregulated pilus (TCP) and the products of the V. cholerae tolQRA genes. Here, we have explored the role of OrfU, a predicted CTXphi minor coat protein, in CTXphi infection. Pr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.3.1037-1044.2003
更新日期:2003-02-01 00:00:00
abstract::Trichloroethylene (TCE) is the most frequently detected groundwater contaminant, and 1-naphthol is an important chemical manufacturing intermediate. Directed evolution was used to increase the activity of toluene ortho-monooxygenase (TOM) of Burkholderia cepacia G4 for both chlorinated ethenes and naphthalene oxidatio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.2.344-349.2002
更新日期:2002-01-01 00:00:00
abstract::Rubrerythrin was purified by multistep chromatography under anaerobic, reducing conditions from the hyperthermophilic archaeon Pyrococcus furiosus. It is a homodimer with a molecular mass of 39.2 kDa and contains 2.9 +/- 0.2 iron atoms per subunit. The purified protein had peroxidase activity at 85 degrees C using hyd...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.23.7888-7895.2004
更新日期:2004-12-01 00:00:00
abstract::In the course of a study of genes located at min 44 of the Escherichia coli genome, we identified an open reading frame with the capacity to encode a 43-kDa polypeptide whose predicted amino acid sequence is strikingly similar to those of the well-known DD-carboxipeptidases penicillin-binding proteins PBP5 and PBP6. T...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.24.7106-7111.1996
更新日期:1996-12-01 00:00:00
abstract::Cybulska, Janina (State Institute of Hygiene, Warsaw, Poland), and J. Jeljaszewicz. Bacteriostatic activity of serum against staphylococci. J. Bacteriol. 91:953-962. 1966.-Antistaphylococcal activity of normal serum against strains exhibiting various patterns of coagulase, clumping-factor, and staphylokinase productio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.3.953-962.1966
更新日期:1966-03-01 00:00:00
abstract::In Salmonella enterica serovar Typhimurium, the stationary-phase sigma factor sigma(S) (RpoS) is required for virulence, stress resistance, biofilm formation, and development of the rdar morphotype. This morphotype is a multicellular behavior characterized by expression of the adhesive extracellular matrix components ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00033-06
更新日期:2006-06-01 00:00:00
abstract::Cytotoxicity is an important virulence determinant in the pathogenesis of Vibrio vulnificus, and two cytotoxins, RTX (encoded by rtxA1) and cytolysin/hemolysin (encoded by vvhA), have been identified in this organism. We showed that the quorum-sensing regulator LuxO controlled the cytotoxicity of this organism: a Δlux...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01259-10
更新日期:2011-05-01 00:00:00
abstract::Rhodococcus sp. strain DK17 is capable of utilizing various derivatives of benzene and bicyclics containing both aromatic and alicyclic moieties as sole carbon and energy sources. Here, we present the 9,107,362-bp draft genome sequence of DK17 and its genomic analysis in comparison with other members of the genus Rhod...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00844-12
更新日期:2012-08-01 00:00:00
abstract::Transmission electron microscopy was used to examine active ribosomal ribonucleic acid (rRNA) genes in two strains of Escherichia coli: N2077, deficient in the enzyme responsible for proper cleavage of the 16S sequence from the elongating nascent rRNA transcript; and N2076, functional in ribonuclease (RNase) III activ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.132.2.718-722.1977
更新日期:1977-11-01 00:00:00
abstract::Burkholderia sp. strain GG4, isolated from the ginger rhizosphere, possesses a unique N-acylhomoserine lactone (AHL)-modifying activity that reduces 3-oxo-AHLs to 3-hydroxy-AHLs. To the best of our knowledge, this is the first sequenced genome from a bacterium of the genus Burkholderia that shows both quorum-sensing a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01578-12
更新日期:2012-11-01 00:00:00
abstract::Henle, Gertrude (Children's Hospital of Philadelphia, Philadelphia, Pa.), and Werner Henle. Immunofluorescence in cells derived from Burkitt's lymphoma. J. Bacteriol. 91:1248-1256. 1966.-Indirect immunofluorescence tests led to the brilliant staining of a small proportion of the cells in five different cultures derive...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.3.1248-1256.1966
更新日期:1966-03-01 00:00:00
abstract::The protein motive force of metabolizing Bacillus subtilis cells was only slightly affected by changes in the external pH between 5 and 8, although the electrical component and the chemical component of the proton motive force contributed differently at different external pH. The electrical component of the proton mot...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.144.3.891-897.1980
更新日期:1980-12-01 00:00:00
abstract::The deoxyribonucleic acid (DNA) polymerase activities in Bacillus subtilis strains Marburg 168 (thy-trp2) and D22, a DNA polymerase I-deficient mutant, were measured at various stages of sporulation. The DNA polymerase I activity, which had decreased after the exponential growth, began to increase at the early stage o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.128.1.221-227.1976
更新日期:1976-10-01 00:00:00
abstract::Vibrio cholerae hemolysin (HlyA) is a 65-kDa water-soluble pore-forming toxin that causes lysis of eukaryotic cells by destroying selective permeability of the plasma membrane bilayer. The HlyA monomer self-assembles on the target cell surface to the more stable beta-barrel amphipathic heptamer, which inserts into the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00930-09
更新日期:2010-01-01 00:00:00
abstract::Eight mutants of Bacillus subtilis deficient in xylan utilization were isolated and characterized genetically and biochemically. Each mutant was obtained independently after nitrosoguanidine mutagenesis. All of the analyzed mutations were shown to be linked. Reciprocal transformation crosses revealed the existence of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.156.1.257-263.1983
更新日期:1983-10-01 00:00:00