Repair of extensive ionizing-radiation DNA damage at 95 degrees C in the hyperthermophilic archaeon Pyrococcus furiosus.

Abstract:

:We investigated the capacity of the hyperthermophile Pyrococcus furiosus for DNA repair by measuring survival at high levels of 60Co gamma-irradiation. The P. furiosus 2-Mb chromosome was fragmented into pieces ranging from 500 kb to shorter than 30 kb at a dose of 2,500 Gy and was fully restored upon incubation at 95 degrees C. We suggest that recombination repair could be an extremely active repair mechanism in P. furiosus and that it might be an important determinant of survival of hyperthermophiles at high temperatures.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

DiRuggiero J,Santangelo N,Nackerdien Z,Ravel J,Robb FT

doi

10.1128/jb.179.14.4643-4645.1997

subject

Has Abstract

pub_date

1997-07-01 00:00:00

pages

4643-5

issue

14

eissn

0021-9193

issn

1098-5530

journal_volume

179

pub_type

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