Visualization of ribosomal ribonucleic acid synthesis in a ribonuclease III-Deficient strain of Escherichia coli.

abstract：:The lactose-H+ symport protein (LacS) of Streptococcus thermophilus has a carboxyl-terminal regulatory domain (IIALacS) that is homologous to a family of proteins and protein domains of the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) in various organisms, of which IIAGlc of Escherichia coli is the...

journal_title：Journal of bacteriology

authors： Gunnewijk MG,Postma PW,Poolman B

更新日期：1999-01-01 00:00:00

abstract：:Cell-free extracts of Thermus thermophilus HB8 catalyze the in vitro, NADH-dependent reduction of potassium tellurite (K2TeO3). Three different protein fractions with tellurite-reducing activities were identified. Two exhibited high molecular weight and were composed of at least two different polypeptides. The protein...

journal_title：Journal of bacteriology

authors： Chiong M,González E,Barra R,Vásquez C

更新日期：1988-07-01 00:00:00

abstract：:The activity of bacterial alkaline phosphatase (PhoA) is dependent on it being exported across the plasma membrane. A plasmid vector (pJEM11) allowing fusions between phoA and genes encoding exported proteins was constructed to study protein export in mycobacteria. Introduction of the Mycobacterium fortuitum beta-lact...

journal_title：Journal of bacteriology

authors： Lim EM,Rauzier J,Timm J,Torrea G,Murray A,Gicquel B,Portnoi D

更新日期：1995-01-01 00:00:00

abstract：:Glycogen accumulation in Escherichia coli is inversely related to the growth rate and occurs most actively when cells enter the stationary phase. The levels of the three biosynthetic enzymes undergo corresponding changes under these conditions, suggesting that genetic control of enzyme biosynthesis may account for at ...

journal_title：Journal of bacteriology

authors： Romeo T,Preiss J

更新日期：1989-05-01 00:00:00

abstract：:Type IV secretion systems (T4SS) mediate the transfer of DNA and protein substrates to target cells. TrwK, encoded by the conjugative plasmid R388, is a member of the VirB4 family, comprising the largest and most conserved proteins of T4SS. VirB4 was suggested to be an ATPase involved in energizing pilus assembly and ...

journal_title：Journal of bacteriology

authors： Arechaga I,Peña A,Zunzunegui S,del Carmen Fernández-Alonso M,Rivas G,de la Cruz F

更新日期：2008-08-01 00:00:00

abstract：:Mutants that require exogenous D-glucosamine for growth were isolated from Saccharomyces cerevisiae X2180-1A after ethyl methane sulfonate mutagenesis. Class A auxotrophs fail to grow on yeast extract-peptone-dextrose and minimal media, whereas class B auxotrophs grow on minimal medium and readily revert to grow on ye...

journal_title：Journal of bacteriology

authors： Whelan WL,Ballou CE

更新日期：1975-12-01 00:00:00

abstract：:We identified several promoters responsible for the expression of regA, which encodes a global virulence regulator in Citrobacter rodentium. Expression of some of the promoters was strongly autoactivated by RegA in conjunction with bicarbonate. Biochemical and mutational analyses were used to determine the consensus s...

journal_title：Journal of bacteriology

authors： Tan A,Yang J,Tauschek M,Praszkier J,Robins-Browne RM

更新日期：2011-04-01 00:00:00

abstract：:The presence of plasmid gene cadB did not affect Cd2+ accumulation, whereas plasmid gene cadA reduced Cd2+ accumulation by whole cells but not by membrane vesicles. Membrane vesicle studies indicated that Cd2+ uptake occurred via the Mn2+ transport system which was energized by the membrane electrical potential. Mn2+ ...

journal_title：Journal of bacteriology

authors： Perry RD,Silver S

更新日期：1982-05-01 00:00:00

abstract：:Pyridine nucleotide-dependent glucose dehydrogenase activity (GPND) is described for the first time in cell-free extracts of certain blue-green algae. When glucose is added to these crude cell extracts, nicotinamide adenine dinucleotide phosphate is reduced at twice the rate as nicotinamide adenine dinucleotide; but e...

journal_title：Journal of bacteriology

authors： Pulich WM,Van Baalen C

更新日期：1973-04-01 00:00:00

abstract：:Hottle, G. A. (Naval Biological Laboratory, University of California, Berkeley), and D. N. Wright. Growth and survival of Mycoplasma neurolyticum in liquid media. J. Bacteriol. 91:1834-1839. 1966.-Maximal growth of Mycoplasma neurolyticum (between 10(8) and 10(9) colony-forming units per ml) was obtained after 3 days ...

journal_title：Journal of bacteriology

authors： Hottle GA,Wright DN

更新日期：1966-05-01 00:00:00

abstract：:A mutant (pgi) of Coprinus macrorhizus deficient in phosphoglucose isomerase did not grow on fructose and grew poorly on glucose. The pgi mutation inhibited the formation of monokaryotic and dikaryotic fruiting bodies. ...

journal_title：Journal of bacteriology

authors： Nyunoya H,Ishikawa T

更新日期：1980-10-01 00:00:00

abstract：:In Bacillus subtilis, the ermC gene encodes an mRNA that is unusually stable (40-min half-life) in the presence of erythromycin, an inducer of ermC gene expression. A requirement for this induced mRNA stability is a ribosome stalled in the ermC leader region. This property of ermC mRNA was used to study the decay of m...

journal_title：Journal of bacteriology

authors： Bechhofer DH,Zen KH

更新日期：1989-11-01 00:00:00

abstract：:The 5,10-methylenetetrahydrofolate dehydrogenase of heterotrophically grown Peptostreptococcus productus Marburg was purified to apparent homogeneity. The purified enzyme catalyzed the reversible oxidation of methylenetetrahydrofolate with NADP+ as the electron acceptor at a specific activity of 627 U/mg of protein. T...

journal_title：Journal of bacteriology

authors： Wohlfarth G,Geerligs G,Diekert G

更新日期：1991-02-01 00:00:00

abstract：:The growth of strain araC(c)67, which produces the enzymes of the ara operon constitutively, is inhibited by the addition of ribitol. Isolation of strains resistant to ribitol yields mutants of either the araB or araC genes. A model to account for the inhibition by ribitol is discussed. ...

journal_title：Journal of bacteriology

authors： Katz L

更新日期：1970-05-01 00:00:00

abstract：:Rhodocyclales are representative of versatile bacteria that are able to utilize a wide variety of organic compounds for growth, but only a few strains have been isolated in pure culture thus far. Here we present the genome sequence of Methyloversatilis universalis FAM5(T), the first cultivable methylotrophic member of...

journal_title：Journal of bacteriology

authors： Kittichotirat W,Good NM,Hall R,Bringel F,Lajus A,Médigue C,Smalley NE,Beck D,Bumgarner R,Vuilleumier S,Kalyuzhnaya MG

更新日期：2011-09-01 00:00:00

abstract：:The mechanisms involved in the virulence of Yersinia pestis, the plague pathogen, are not fully understood. In previous research, we found that a Y. pestis mutant lacking the HicB3 (YPO3369) putative orphan antitoxin was attenuated for virulence in a murine model of bubonic plague. Toxin-antitoxin systems (TASs) are w...

journal_title：Journal of bacteriology

authors： Bibi-Triki S,Li de la Sierra-Gallay I,Lazar N,Leroy A,Van Tilbeurgh H,Sebbane F,Pradel E

更新日期：2014-11-01 00:00:00

abstract：:Two of the three plasmids of the wild-type Rhizobium meliloti 41 (pRme41a and pRme41c) carry a copy of ISRm2, a 2.7-kilobase-long transposable element. ISRm2 is terminated by 22-base-pair (bp) inverted repeat sequences, exhibiting some homology to the inverted repeats of elements generating 9-bp target sequence duplic...

journal_title：Journal of bacteriology

authors： Dusha I,Kovalenko S,Banfalvi Z,Kondorosi A

更新日期：1987-04-01 00:00:00

abstract：:mRNA levels from the bop (bacterio-opsin), brp (bacterio-opsin-related protein), and bat (bacterio-opsin activator) genes in wild-type Halobacterium halobium and two bacteriorhodopsin-overproducing mutants (ET1001 and II-7) were quantitated under conditions in which oxygen levels were steadily depleted and then cultur...

journal_title：Journal of bacteriology

authors： Shand RF,Betlach MC

更新日期：1994-03-01 00:00:00

abstract：:The metE gene of Salmonella typhimurium was cloned into plasmid pACYC184 by using a lambda gt7 -metE transducing phage as a source of metE DNA. The recombinant plasmid, designated pGS41 , carries a 12.8-kilobase-pair EcoRI insert fragment. The metE gene was subcloned from pGS41 into plasmid pBR322 on a 4.2-kilobase-pa...

journal_title：Journal of bacteriology

authors： Schulte LL,Stauffer LT,Stauffer GV

更新日期：1984-06-01 00:00:00

abstract：:Entomopathogenic bacteria of the genus Photorhabdus harbor a type III secretion system. This system was probably acquired prior to the separation of the species within this genus. Furthermore, the core components of the secretion machinery are highly conserved but the predicted effectors differ between Photorhabdus lu...

journal_title：Journal of bacteriology

authors： Brugirard-Ricaud K,Givaudan A,Parkhill J,Boemare N,Kunst F,Zumbihl R,Duchaud E

更新日期：2004-07-01 00:00:00

abstract：:Bacillus subtilis DegS-DegU belongs to a bacterial two-component system that controls many processes, including the production of exocellular proteases and competence development. It was found that when the glutamine synthetase gene glnA, which is involved in nitrogen regulation, was disrupted, the expression of the r...

journal_title：Journal of bacteriology

authors： Yasumura A,Abe S,Tanaka T

更新日期：2008-08-01 00:00:00

abstract：:A new class II (Tn3-like) transposable element, designated Tn5401, was recovered from a sporulation-deficient variant of Bacillus thuringiensis subsp. morrisoni EG2158 following its insertion into a recombinant plasmid. Sequence analysis of the insert revealed a 4,837-bp transposon with two large open reading frames, ...

journal_title：Journal of bacteriology

authors： Baum JA

更新日期：1994-05-01 00:00:00

abstract：:Two low-molecular-weight basic proteins, termed A and B proteins, comprise about 15% of the protein of dormant spores of Bacillus megaterium. Irradiation of intact dormant spores with ultraviolet light results in covalent cross-linking of the A and B proteins to other spore macromolecules. The cross-linked A and B pro...

journal_title：Journal of bacteriology

authors： Setlow B,Setlow P

更新日期：1979-08-01 00:00:00

abstract：:The presence of the altered lysine transfer ribonucleic acid (tRNA) in Bacillus subtilis spores is strongly dependent on the medium on which the cells were sporulated. Cells sporulated on synthetic media or dilute complex media contain little or none of the new component, whereas those sporulated on concentrated compl...

journal_title：Journal of bacteriology

authors： Lazzarini RA,Santangelo E

更新日期：1967-07-01 00:00:00

abstract：:Deoxyribonucleic acid repair synthesis was studied in one wild-type and two mutant strains of Bacillus subtilis that are defective in excision of pyrimidine dimers. The cells were irradiated with ultraviolet light, and 6-(p-hydroxyphenyl-azo)-uracil was used to block replicative synthesis, allowing only repair synthes...

journal_title：Journal of bacteriology

authors： Hadden CT

更新日期：1979-07-01 00:00:00

abstract：:Limited proteolysis of the Escherichia coli cell division inhibitor MinC reveals that its dimerization function resides in a structurally autonomous C-terminal domain. We show that cytoplasmic MinC is poised near the monomer-dimer equilibrium and propose that it only becomes entirely dimeric once recruited to the memb...

journal_title：Journal of bacteriology

authors： Szeto TH,Rowland SL,King GF

更新日期：2001-11-01 00:00:00

abstract：:An unusual xylose isomerase produced by Thermoanaerobacterium strain JW/SL-YS 489 was purified 28-fold to gel electrophoretic homogeneity, and the biochemical properties were determined. Its pH optimum distinguishes this enzyme from all other previously described xylose isomerases. The purified enzyme had maximal acti...

journal_title：Journal of bacteriology

authors： Liu SY,Wiegel J,Gherardini FC

更新日期：1996-10-01 00:00:00

abstract：:By means of the general procedure of Casadaban (J. Mol. Biol. 104: 541-556, 1976), the lac genes carried on a lambda-Mu-1 hybrid phage were inserted into a temperature-inducible Mu-1 prophage that had earlier been inserted into a site near the beginning of the ilvC gene of Escherichia coli strain K-12. Selection of te...

journal_title：Journal of bacteriology

authors： Smith JM,Umbarger HE

更新日期：1977-12-01 00:00:00

abstract：:We examined the predacious gram-negative bacterium Bdellovibrio bacteriovorous 109J and free-living strains 109J-A1 and 109J-KA1 derived therefrom for penicillin-binding proteins (PBPs). We compared their PBPs with those of the host bacterium, Escherichia coli, and with those of a facultatively predacious bdellovibrio...

journal_title：Journal of bacteriology

authors： Park JT,Mahadevan S

更新日期：1988-08-01 00:00:00

abstract：:In Bacillus subtilis the deoxyribonucleic acid content and the extent of cell division during inhibition of chromosome replication increased as a function of the average cell mass, independent of the growth rate. At each growth rate, mass, deoxyribonucleic acid, and residual division varied in different cultures. The ...

journal_title：Journal of bacteriology

authors： Holmes M,Rickert M,Pierucci O

更新日期：1980-04-01 00:00:00