Cadmium and manganese transport in Staphylococcus aureus membrane vesicles.

Abstract:

:The presence of plasmid gene cadB did not affect Cd2+ accumulation, whereas plasmid gene cadA reduced Cd2+ accumulation by whole cells but not by membrane vesicles. Membrane vesicle studies indicated that Cd2+ uptake occurred via the Mn2+ transport system which was energized by the membrane electrical potential. Mn2+ and Cd2+ were competitive inhibitors of each other's transport, with Km's of 0.95 microM Mn2+ and 0.2 microM Cd2+. The kinetic parameters were nearly identical with vesicles prepared from sensitive and resistant cells, indicating that the cadA-encoded Cd2+ efflux system was inoperative in membrane vesicle preparations. Experiments with energy-inhibited cells indicated that the cadB gene product may bind Cd2+.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Perry RD,Silver S

doi

10.1128/JB.150.2.973-976.1982

subject

Has Abstract

pub_date

1982-05-01 00:00:00

pages

973-6

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

150

pub_type

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