Replication of the R factor Rts1 in Proteus mirabilis.

abstract：:In previous work, we characterized the bases in an iteron of plasmid R6K that are important for the binding of pi protein monomers and dimers. Here we investigate the following six amino acids of pi, encoded by pir, hypothesized to be important for DNA contact: Ser71, Try74, Gly131, Gly211, Arg225, and Arg254. ...

journal_title：Journal of bacteriology

authors： Kunnimalaiyaan S,Rakowski SA,Filutowicz M

更新日期：2007-07-01 00:00:00

abstract：:Doxtader, K. G. (Cornell University, Ithaca, N.Y.), and M. Alexander. Role of 3-nitropropanoic acid in nitrate formation by Aspergillus flavus. J. Bacteriol. 91:1186-1191. 1966.-Aspergillus flavus formed nitrate, 3-nitropropanoic acid (3-NPA), kojic acid, and a substance tentatively identified as N-formyl-N-hydroxy-gl...

journal_title：Journal of bacteriology

authors： Doxtader KG,Alexander M

更新日期：1966-03-01 00:00:00

abstract：:A reaction center H- strain (RCH-) of Rhodobacter sphaeroides, PUHA1, was made by in vitro deletion of an XhoI restriction endonuclease fragment from the puhA gene coupled with insertion of a kanamycin resistance gene cartridge. The resulting construct was delivered to R. sphaeroides wild-type 2.4.1, with the defectiv...

journal_title：Journal of bacteriology

authors： Sockett RE,Donohue TJ,Varga AR,Kaplan S

更新日期：1989-01-01 00:00:00

abstract：:A pLAFR3 cosmid clone designated pVir2 containing a 25-kilobase (kb) DNA insert was isolated from a wild-type Pseudomonas solanacearum GMI1000 genomic library. This cosmid was shown to complement all but one of the nine Tn5-induced mutants which have been isolated after random mutagenesis and which have lost both path...

journal_title：Journal of bacteriology

authors： Boucher CA,Van Gijsegem F,Barberis PA,Arlat M,Zischek C

更新日期：1987-12-01 00:00:00

abstract：:The genome sequences of several pseudomonads have revealed a gene cluster containing genes for a two-component heavy metal histidine sensor kinase and response regulator upstream of cinA and cinQ, which we show herein to encode a copper-containing azurin-like protein and a pre-Q(0) reductase, respectively. In the pres...

journal_title：Journal of bacteriology

authors： Quaranta D,McCarty R,Bandarian V,Rensing C

更新日期：2007-07-01 00:00:00

abstract：:The AraC homolog ToxT coordinately regulates virulence gene expression in Vibrio cholerae. ToxT is required for transcriptional activation of the genes encoding cholera toxin and the toxin coregulated pilus, among others. In this work we focused on the interaction of ToxT with the tcpA promoter and investigated the me...

journal_title：Journal of bacteriology

authors： Hulbert RR,Taylor RK

更新日期：2002-10-01 00:00:00

abstract：:We constructed a shuttle vector, pE5-2, which can replicate in both Bacteroides spp. and Escherichia coli. pE5-2 contains a cryptic Bacteroides plasmid (pB8-51), a 3.8-kilobase (kb) EcoRI-D fragment from the 41-kb Bacteroides fragilis plasmid pBF4, and RSF1010, an IncQ E. coli plasmid. pE5-2 was mobilized by R751, an ...

journal_title：Journal of bacteriology

authors： Shoemaker NB,Guthrie EP,Salyers AA,Gardner JF

更新日期：1985-05-01 00:00:00

abstract：:D-[alpha-14C]]glucosyl phosphorylpolyprenol ([ 14C]Glc-P-prenol) was formed from UDP-D-[14C]glucose in each of the membrane systems obtained from Bacillus coagulans AHU 1631 and AHU 1634 and two Bacillus megaterium strains. Membranes of these B. coagulans strains, which possess beta-D-glucosyl branches on the repeatin...

journal_title：Journal of bacteriology

authors： Shimada A,Tamatukuri J,Ito E

更新日期：1989-05-01 00:00:00

abstract：:Pyridine-2,6-bis(thiocarboxylate) (PDTC), produced by certain pseudomonads, is a sulfur-containing siderophore that binds iron, as well as a wide range of transition metals, and it affects the net hydrolysis of the environmental contaminant carbon tetrachloride. The pathway of PDTC biosynthesis has not been defined. H...

journal_title：Journal of bacteriology

authors： Lewis TA,Glassing A,Harper J,Franklin MJ

更新日期：2013-09-01 00:00:00

abstract：:The nucleic acid binding cold shock proteins (CSPs) and the cold-induced DEAD box RNA helicases have been proposed separately to act as RNA chaperones, but no experimental evidence has been reported on a direct cooperation. To investigate the possible interaction of the putative RNA helicases CshA and CshB and the CSP...

journal_title：Journal of bacteriology

authors： Hunger K,Beckering CL,Wiegeshoff F,Graumann PL,Marahiel MA

更新日期：2006-01-01 00:00:00

abstract：:A low level of recombination between homologous regions of plasmids and the host chromosome occurred during cell growth. The plasmids contained antibiotic resistance markers on the homologous regions which were only expressed when they were integrated into the chromosome. Such recombination took place in Rec+ rec-2 mu...

journal_title：Journal of bacteriology

authors： Balganesh M,Arrigoni L,Setlow JK

更新日期：1986-10-01 00:00:00

abstract：:HetR plays a key role in regulation of heterocyst differentiation and patterning in Anabaena It directly regulates genes involved in heterocyst differentiation (such as hetP and hetZ), genes involved in pattern formation (patA), and many others. In this study, we investigated the functional relationship of hetP and he...

journal_title：Journal of bacteriology

authors： Zhang H,Wang S,Wang Y,Xu X

更新日期：2018-04-09 00:00:00

abstract：:The biodegradative threonine dehydratase (tdc) operon was mapped at 68 min on the Escherichia coli K-12 chromosome. The order of markers in the clockwise direction was dnaG uxaA tdc argG. A tdc deletion was isolated and mapped to this region of the chromosome. By using a tdcB-lacZ fusion the clockwise direction of tra...

journal_title：Journal of bacteriology

authors： Schweizer HP,Datta P

更新日期：1988-11-01 00:00:00

abstract：:Bacillus subtilis has developed an intricate signal transduction cascade to respond to the imposition of a variety of stresses on the cell. Reversible protein phosphorylation and the formation of alternative protein-protein complexes modulate the activity of sigma(B), the RNA polymerase sigma factor subunit responsibl...

journal_title：Journal of bacteriology

authors： Delumeau O,Chen CC,Murray JW,Yudkin MD,Lewis RJ

更新日期：2006-11-01 00:00:00

abstract：:Supplementation of the growth medium with high concentrations of NaCl, KCl, or sucrose caused a drastic change in the ratio of the two peptidoglycan-associated major outer membrane proteins of Escherichia coli K-12 in that the amounts of proteins b and c present in cell envelope preparations decreased and increased, r...

journal_title：Journal of bacteriology

authors： Alphen WV,Lugtenberg B

更新日期：1977-08-01 00:00:00

abstract：:The crystal structure of Escherichia coli PdxY, the protein product of the pdxY gene, has been determined to a 2.2-A resolution. PdxY is a member of the ribokinase superfamily of enzymes and has sequence homology with pyridoxal kinases that phosphorylate pyridoxal at the C-5' hydroxyl. The protein is a homodimer with ...

journal_title：Journal of bacteriology

authors： Safo MK,Musayev FN,Hunt S,di Salvo ML,Scarsdale N,Schirch V

更新日期：2004-12-01 00:00:00

abstract：:The chromosomal DNA replication origins (oriC) from two members of the family Enterobacteriaceae, Enterobacter aerogenes and Klebsiella pneumoniae, have been isolated as functional replication origins in Escherichia coli. The origins in the SalI restriction fragments of 17.5 and 10.2 kilobase pairs, cloned from E. aer...

journal_title：Journal of bacteriology

authors： Harding NE,Cleary JM,Smith DW,Michon JJ,Brusilow WS,Zyskind JW

更新日期：1982-12-01 00:00:00

abstract：:A Pediococcus cerevisiae mutant that actively accumulated folate (PteGlu), in contrast to the wild-type, was also found to exhibit changes in the pattern of uptake of 5-methyl-tetrahydrofolate (5-CH3-H4PteGlu) and amethopterin. Most of the 5-CH3-H4PteGlue accumulated through a glucose- and temperature-dependent proces...

journal_title：Journal of bacteriology

authors： Mandelbaum-Shavit F,Grossowicz N

更新日期：1975-08-01 00:00:00

abstract：:Pseudomonas MS can grow on methylamine and a number of other compounds containing C1 units as a sole source of carbon and energy. Assimilation of carbon into cell material occurs via the "serine pathway" since enzymes of this pathway are induced after growth on methylamine, but not malate or acetate. A mutant has been...

journal_title：Journal of bacteriology

authors： Wagner C,Levitch ME

更新日期：1975-06-01 00:00:00

abstract：:Two distinct phenotypic classes of lysine requiring auxotrophs of Escherichia coli are described. Mutants of the LysA class produce little or no active diaminopimelic acid (DAP) decarboxylase and specifically require lysine for growth. Mutants of the LysB class produce a cryptic DAP decarboxylase which can be activate...

journal_title：Journal of bacteriology

authors： Bukhari AI,Taylor AL

更新日期：1971-03-01 00:00:00

abstract：:Inhibition of deoxyribonucleic acid (DNA) synthesis in Escherichia coli by chemical inhibitors or by shifting cultures of temperature-sensitive elongation (dnaE and dnaB) or initiation (dnaA) mutants to nonpermissive conditions led to greatly increased synthesis of the enzyme ribonucleoside diphosphate reductase, whic...

journal_title：Journal of bacteriology

authors： Filpula D,Fuchs JA

更新日期：1977-04-01 00:00:00

abstract：:D-Glucose dehydrogenase is a pyrroloquinoline quinone-dependent primary dehydrogenase linked to the respiratory chain of a wide variety of bacteria. The enzyme exists in the membranes of Escherichia coli, mainly as an apoenzyme which can be activated by the addition of pyrroloquinoline quinone and magnesium. Thus, mem...

journal_title：Journal of bacteriology

authors： Matsushita K,Nonobe M,Shinagawa E,Adachi O,Ameyama M

更新日期：1987-01-01 00:00:00

abstract：:Group B streptococcus (GBS) pili may enhance colonization and infection by mediating bacterial adhesion to host cells, invasion across endothelial and epithelial barriers, and resistance to bacterial ingestion and killing by host phagocytes. However, it remains unclear how pilus expression is regulated and how modulat...

journal_title：Journal of bacteriology

authors： Jiang S,Park SE,Yadav P,Paoletti LC,Wessels MR

更新日期：2012-05-01 00:00:00

abstract：:SAL-TOL in vivo recombinant plasmid pKF439 was characterized in a strain from a mixed culture of bacteria harboring various degradative plasmids. Analysis of the gene organization of pKF439 revealed that the 57-kilobase TOL fragment, including the 40-kilobase TOL metabolic region, was inserted into the complete SAL re...

journal_title：Journal of bacteriology

authors： Furukawa K,Miyazaki T,Tomizuka N

更新日期：1985-06-01 00:00:00

abstract：:The vir-type IV secretion system of Agrobacterium is assembled from 12 proteins encoded by the virB operon and virD4. VirB1 is one of the least-studied proteins encoded by the virB operon. Its N terminus is a lytic transglycosylase. The C-terminal third of the protein, VirB1*, is cleaved from VirB1 and secreted to the...

journal_title：Journal of bacteriology

authors： Zupan J,Hackworth CA,Aguilar J,Ward D,Zambryski P

更新日期：2007-09-01 00:00:00

abstract：:Holins are a diverse group of small integral membrane proteins elaborated by bacteriophages to lyse bacterial hosts and effect release of progeny phages in a precisely timed manner. Recently, the holin S gene of phage lambda was overexpressed and the holin protein was purified to homogeneity by means of an oligohistid...

journal_title：Journal of bacteriology

authors： Smith DL,Young R

更新日期：1998-08-01 00:00:00

abstract：:Sequencing 16S rRNA genes (SSU) cloned from Aeromonas strains revealed that strains contained up to six copies differing by < or = 1.5%. The SSU copies from Aeromonas veronii LMG13695 clustered with sequences from four Aeromonas species. These results demonstrate intragenomic heterogeneity of SSU and suggest caution w...

journal_title：Journal of bacteriology

authors： Morandi A,Zhaxybayeva O,Gogarten JP,Graf J

更新日期：2005-09-01 00:00:00

abstract：:We previously reported that group D streptococci exhibited immunoglobulin G (IgG)-binding activity and that a 52-kDa IgG-binding protein was present in all Streptococcus suis strains examined (B. Serhir, R. Higgins, B. Foiry, and M. Jacques, J. Gen. Microbiol. 139:2953-2958, 1993). The objective of the present study w...

journal_title：Journal of bacteriology

authors： Serhir B,Dubreuil D,Higgins R,Jacques M

更新日期：1995-07-01 00:00:00

abstract：:The recA gene from Thermus thermophilus HB27 was cloned and engineered to obtain insertion (recA::kat) and deletion (deltarecA) derivatives. Transcription of recA in this extreme thermophile was induced by mitomycin C, leading to the synthesis of a monocistronic mRNA. This DNA damage-mediated induction was dependent o...

journal_title：Journal of bacteriology

authors： Castán P,Casares L,Barbé J,Berenguer J

更新日期：2003-08-01 00:00:00

abstract：:Forty-five strains, representing 18 species of the genus Bacillus, were surveyed for production of hemolysin against rabbit erythrocytes. Broth cultures of B. cereus, B. alvei, and B. laterosporus contained lysins that closely resembled streptolysin O. B. subtilis and a single strain of B. cereus may produce lysins ha...

journal_title：Journal of bacteriology

authors： Bernheimer AW,Grushoff P

更新日期：1967-05-01 00:00:00