New methionine structural gene in Salmonella typhimurium.

Abstract:

:Eight metH mutants in Salmonella typhimurium with closely linked sites of mutation which could grow only on methionine were isolated from a metE mutant deficient in N(5)-methyltetrahydropteroyltriglutamate-homocysteine transmethylase; their deficiency in cobalamin-dependent N(5)-methyltetrahydrofolate-homocysteine transmethylase was supported by the results of enzyme studies of one of them. Cotransduction of metH and metA (homoserine O-transsuccinylase) mutants was obtained, thus revealing linkage between a second pair of the six known methionine structural genes. One metH mutant clearly differed from the rest in that it reverted at a higher frequency, was temperature sensitive, complemented all other metH mutants, and was located farthest from the metA gene.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Childs JD,Smith DA

doi

10.1128/JB.100.1.377-382.1969

subject

Has Abstract

pub_date

1969-10-01 00:00:00

pages

377-82

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

100

pub_type

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