The interaction of T4 endonuclease V E23Q mutant with thymine dimer- and tetrahydrofuran-containing DNA.

abstract：:The herpes simplex virus thymidine kinase gene has been cloned into a chimeric yeast plasmid cloning vehicle and transformed into appropriate yeast strains. Plasmids carrying the herpes simplex virus thymidine kinase gene can be propagated as autonomously replicating plasmids, but no RNA specific to the thymidine kina...

journal_title：Journal of bacteriology

authors： Kiss GB,Pearlman RE,Cornish KV,Friesen JD,Chan VL

更新日期：1982-02-01 00:00:00

abstract：:The ability to form type 1 fimbriae (Fim(+)) was readily transduced to 159 out of 161 wild-type motile Fim(-) FIRN strains of Salmonella typhimurium with phage P22 propagated on a Fim(+) donor strain. Fim(+) clones were isolated from about 35% of tests after the fimbriate bacteria in the transduction mixture had been ...

journal_title：Journal of bacteriology

authors： Old DC,Duguid JP

更新日期：1971-09-01 00:00:00

abstract：:A handful of nucleoid-associated proteins (NAPs) regulate the vast majority of genes in a bacterial cell. H-NS, the histone-like nucleoid-structuring protein, is one of these NAPs and protects Escherichia coli from foreign gene expression. Though lacking any sequence similarity with E. coli H-NS, Rv3852 was annotated ...

journal_title：Journal of bacteriology

authors： Odermatt NT,Sala C,Benjak A,Kolly GS,Vocat A,Lupien A,Cole ST

更新日期：2017-07-25 00:00:00

abstract：:The Yersinia adhesin A (YadA) is a trimeric autotransporter adhesin of enteric yersiniae. It consists of three major domains: a head mediating adherence to host cells, a stalk involved in serum resistance, and an anchor that forms a membrane pore and is responsible for the autotransport function. The anchor contains a...

journal_title：Journal of bacteriology

authors： Grosskinsky U,Schütz M,Fritz M,Schmid Y,Lamparter MC,Szczesny P,Lupas AN,Autenrieth IB,Linke D

更新日期：2007-12-01 00:00:00

abstract：:Mutation of a conserved His-157 in the second pore loop of KtrB drastically reduced the activity of the K+ transporter from Synechocystis sp. strain PCC 6803. This result suggests that His-157 plays an essential role in the K+ transport activity of the transporter system. ...

journal_title：Journal of bacteriology

authors： Zulkifli L,Uozumi N

更新日期：2006-11-01 00:00:00

abstract：:IS630 is a 1.15-kilobase sequence in Shigella sonnei that, unlike many mobile elements, seems not to mediate cointegration between different replicons. To assess its transposition, we constructed composite elements containing inverted copies of IS630 flanking a drug resistance gene. We found that these composite eleme...

journal_title：Journal of bacteriology

authors： Tenzen T,Matsutani S,Ohtsubo E

更新日期：1990-07-01 00:00:00

abstract：:Utilization of heme as an iron source by Bradyrhizobium japonicum involves induction of the outer membrane heme receptor gene hmuR and other genes within the heme utilization locus. Here, we discovered the hmuP gene located upstream of hmuR and transcribed divergently from it along with hmuTUV. hmuP encodes a small pr...

journal_title：Journal of bacteriology

authors： Escamilla-Hernandez R,O'Brian MR

更新日期：2012-06-01 00:00:00

abstract：:Activities of gamma-butyrobetaine hydroxylase and carnitine dehydrogenase were low in cells of Pseudomonas sp. AK 1 grown in the absence of their respective substrates. ...

journal_title：Journal of bacteriology

authors： Lindstedt G,Lindstedt S,Midtvedt T,Tofft M

更新日期：1970-03-01 00:00:00

abstract：:Bacteria often encounter environments where nutrient availability is limited, and they must adapt accordingly. To identify Pasteurella multocida genes that are differentially expressed during nutrient limitation, we utilized whole-genome microarrays to compare levels of gene expression during growth in rich and minima...

journal_title：Journal of bacteriology

authors： Paustian ML,May BJ,Kapur V

更新日期：2002-07-01 00:00:00

abstract：:Escherichia coli lactose permease mediates the proton-driven translocation of galactosides across the cytoplasmic membrane. To define regions important for membrane insertion as well as for biological function, we constructed plasmids encoding different portions of the lactose carrier. Among several lacY deletions, tw...

journal_title：Journal of bacteriology

authors： Wrubel W,Stochaj U,Sonnewald U,Theres C,Ehring R

更新日期：1990-09-01 00:00:00

abstract：:Recombination studies of bacteria are often carried out with multicopy plasmids, and recombination frequencies are often deduced from the proportion of cells in the population that express a recombinant phenotype. These frequencies should however be called apparent frequencies, since detection of the recombinant cells...

journal_title：Journal of bacteriology

authors： Chédin F,Dervyn R,Ehrlich SD,Noirot P

更新日期：1997-02-01 00:00:00

abstract：:Listeria monocytogenes is a Gram-positive human intracellular pathogen that infects diverse mammalian cells. Upon invasion, L. monocytogenes secretes multiple virulence factors that target host cellular processes and promote infection. It has been presumed, but was not empirically established, that the Sec translocati...

journal_title：Journal of bacteriology

authors： Burg-Golani T,Pozniak Y,Rabinovich L,Sigal N,Nir Paz R,Herskovits AA

更新日期：2013-12-01 00:00:00

abstract：:When Bacillus subtilis is subjected to phosphate starvation, genes of the Pho regulon are either induced or repressed. Among those induced are genes encoding alkaline phosphatases (APases). A set of isogenic mutants, with a beta-galactosidase gene transcriptionally fused to the inactivated target gene, was used to ide...

journal_title：Journal of bacteriology

authors： Prágai Z,Eschevins C,Bron S,Harwood CR

更新日期：2001-04-01 00:00:00

abstract：:Azotobacter vinelandii produces two detectable catalases during growth on minimal medium. The heat-labile catalase expressed during exponential growth phase was identified as a KatG homologue by liquid chromatography-tandem mass spectrometry (LC-MS/MS) using a mixed protein sample. The second catalase was heat resista...

journal_title：Journal of bacteriology

authors： Sandercock JR,Page WJ

更新日期：2008-02-01 00:00:00

abstract：:Acyl carrier protein (ACP coli) was isolated from commercially grown Escherichia coli B and was acetylated by chemical methods. Biological activity of the synthesized acetyl-ACP coli was checked in an in vitro fatty acid-synthesizing system isolated from E. coli B. Since acetyl-ACP is preferred over acetyl-coenzyme A ...

journal_title：Journal of bacteriology

authors： Marcus SL,Reeves HC,Ajl SJ

更新日期：1968-10-01 00:00:00

abstract：:A key reaction in the biosynthesis of menaquinone involves the conversion of the soluble bicyclic naphthalenoid compound 1, 4-dihydroxy-2-naphthoic acid (DHNA) to the membrane-bound demethylmenaquinone. The enzyme catalyzing this reaction, DHNA-octaprenyltransferase, attaches a 40-carbon side chain to DHNA. The menA g...

journal_title：Journal of bacteriology

authors： Suvarna K,Stevenson D,Meganathan R,Hudspeth ME

更新日期：1998-05-01 00:00:00

abstract：:We have identified recD mutants of Salmonella typhimurium by their ability to support growth of phage P22 abc (anti-RecBCD) mutants, whose growth is prevented by normal host RecBCD function. As in Escherichia coli, the recD gene of S. typhimurium lies between the recB and argA genes at min 61 of the genetic map. Plasm...

journal_title：Journal of bacteriology

authors： Miesel L,Roth JR

更新日期：1994-07-01 00:00:00

abstract：:Cecropins are a family of small, basic antibacterial polypeptides which can be isolated from pupae of immunized Lepidoptera. They are active against both gram-negative and gram-positive bacteria. We studied a mutant of Escherichia coli, strain SB1004, which is more sensitive to cecropin D than is the parental strain. ...

journal_title：Journal of bacteriology

authors： Sidén I,Boman HG

更新日期：1983-04-01 00:00:00

abstract：:Two homologs of the outer membrane protein OmpA were identified in Aeromonas salmonicida by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting, and amino-terminal sequence analyses. An A. salmonicida genomic DNA library was constructed by using lambda GEM-11 and recombinant phage carrying both g...

journal_title：Journal of bacteriology

authors： Costello GM,Vipond R,MacIntyre S

更新日期：1996-03-01 00:00:00

abstract：:We have extended our previous studies of the DNA sequences required for growth rate-dependent control of rRNA transcription in Escherichia coli. Utilizing a reporter system suitable for evaluation of promoters with low activities, we have found that the core promoter region of rrnB P1 (-41 to +1 with respect to the tr...

journal_title：Journal of bacteriology

authors： Bartlett MS,Gourse RL

更新日期：1994-09-01 00:00:00

abstract：:The degradation of synthetic compounds requires bacteria to recruit and adapt enzymes from pathways for naturally occurring compounds. Previous work defined the steps in 2,4-dinitrotoluene (2,4-DNT) metabolism through the ring fission reaction. The results presented here characterize subsequent steps in the pathway th...

journal_title：Journal of bacteriology

authors： Johnson GR,Jain RK,Spain JC

更新日期：2002-08-01 00:00:00

abstract：:The enzymes LinB(UT) and LinB(MI) (LinB from Sphingobium japonicum UT26 and Sphingobium sp. MI1205, respectively) catalyze the hydrolytic dechlorination of β-hexachlorocyclohexane (β-HCH) and yield different products, 2,3,4,5,6-pentachlorocyclohexanol (PCHL) and 2,3,5,6-tetrachlorocyclohexane-1,4-diol (TCDL), respecti...

journal_title：Journal of bacteriology

authors： Okai M,Ohtsuka J,Imai LF,Mase T,Moriuchi R,Tsuda M,Nagata K,Nagata Y,Tanokura M

更新日期：2013-06-01 00:00:00

abstract：:The specific activities of 13 aminoacyl-soluble ribonucleic acid (sRNA) synthetases were measured at various time intervals during the germination of Botryodiplodia theobromae conidiospores. The enzyme activities were low or absent in ungerminated spores, and they increased rapidly as germination proceeded. When extra...

journal_title：Journal of bacteriology

authors： Van Etten JL,Brambl RM

更新日期：1968-10-01 00:00:00

abstract：:The ability of different metal ions to promote transformation of Pseudomonas aeruginosa by deoxyribonucleic acid of the plasmid RP1 was examined. CaCl2, MgCl2, and MnCl2 were found to promote such transformation, although at different frequencies and with the optimum response at different concentrations. Only MgCl2 pr...

journal_title：Journal of bacteriology

authors： Mercer AA,Loutit JS

更新日期：1979-10-01 00:00:00

abstract：:A mutant of Mycobacterium smegmatis unable to use the dipeptide carnosine (beta-alanyl-L-histidine) as a sole carbon or nitrogen source was isolated. Carnosinase activity and the ability to grow on beta-Ala and/or L-His were similar in the mutant and the wild type. However, the mutant showed significant impairment in ...

journal_title：Journal of bacteriology

authors： Bhatt A,Green R,Coles R,Condon M,Connell ND

更新日期：1998-12-01 00:00:00

abstract：:Western blot (immunoblot) analysis of cell extracts from induced bacteriophage lambda lysogens probed with S-protein-specific antibody (raised against an S--beta-galactosidase fusion protein) demonstrated that the bacteriophage lambda S protein begins to appear 10 min after phage induction and is localized to the inne...

journal_title：Journal of bacteriology

authors： Zagotta MT,Wilson DB

更新日期：1990-02-01 00:00:00

abstract：:FabG performs the NADPH-dependent reduction of β-keto acyl-acyl carrier protein substrates in the elongation cycle of fatty acid synthesis. We report the characterization of a temperature-sensitive mutation (fabGΔ8) in Escherichia colifabG that results from an in-frame 8-amino-acid residue deletion in the α6/α7 subdom...

journal_title：Journal of bacteriology

authors： Srinivas S,Cronan JE

更新日期：2017-04-25 00:00:00

abstract：:Chloroethylthiamine was found to inhibit an entrapment of thiamine as thiamine monophosphate by blocking thiamine monophosphokinase in the cytoplasm after thiamine was taken up by the cells of Escherichia coli. ...

journal_title：Journal of bacteriology

authors： Iwashima A,Nose Y

更新日期：1972-12-01 00:00:00

abstract：:A 6.2-kb region of DNA corresponding to complementation groups II and III of the Erwinia amylovora hrp gene cluster was analyzed. Transposon mutagenesis indicated that the two complementation groups are required for secretion of harpin, an elicitor of the hypersensitive reaction. The sequence of the region revealed 10...

journal_title：Journal of bacteriology

authors： Kim JF,Wei ZM,Beer SV

更新日期：1997-03-01 00:00:00

abstract：:Flagellar phase variation of Salmonella is a phenomenon where two flagellin genes, fliC (phase 1) and fljB (phase 2), are expressed alternately. This is controlled by the inversion of a DNA segment containing the promoter for the fljB gene. The fljB gene constitutes an operon with the fljA gene, which encodes a negati...

journal_title：Journal of bacteriology

authors： Yamamoto S,Kutsukake K

更新日期：2006-02-01 00:00:00