Abstract:
:A phosphatase specific for the hydrolysis of 3-deoxy-d-manno-octulosonate (KDO)-8-phosphate was purified approximately 400-fold from crude extracts of Escherichia coli B. The hydrolysis of KDO-8-phosphate to KDO and inorganic phosphate in crude extracts of E. coli B, grown in phosphate-containing minimal medium, could be accounted for by the enzymatic activity of this specific phosphatase. No other sugar phosphate tested was an alternate substrate or inhibitor of the purified enzyme. KDO-8-phosphate phosphatase was stimulated three- to fourfold by the addition of 1.0 mM Co(+) or Mg(2+) and to a lesser extent by 1.0 mM Ba(2+), Zn(2+), and Mn(2+). The activity was inhibited by the addition of 1.0 mM ethylenediaminetetraacetic acid, Cu(2+), Ca(2+), Cd(2+), Hg(2+), and chloride ions (50% at 0.1 M). The pH optimum was determined to be 5.5 to 6.5 in both tris(hydroxymethyl)aminomethane-acetate and HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid) buffer. This specific phosphatase had an isoelectric point of 4.7 to 4.8 and a molecular weight of 80,000 +/- 6,000 as determined by molecular sieving and Ferguson analysis. The enzyme appeared to be composed of two identical subunits of 40,000 to 43,000 molecular weight. The apparent K(m) for KDO-8-phosphate was determined to be 5.8 +/- 0.9 x 10(-5) M in the presence of 1.0 mM Co(2+), 9.1 +/- 1 x 10(-5) M in the presence of 1.0 mM Mg(2+), and 1.0 +/- 0.2 x 10(-4) M in the absence of added Co(2+) or Mg(2+).
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Ray PH,Benedict CDdoi
10.1128/JB.142.1.60-68.1980subject
Has Abstractpub_date
1980-04-01 00:00:00pages
60-8issue
1eissn
0021-9193issn
1098-5530journal_volume
142pub_type
杂志文章abstract::A handful of nucleoid-associated proteins (NAPs) regulate the vast majority of genes in a bacterial cell. H-NS, the histone-like nucleoid-structuring protein, is one of these NAPs and protects Escherichia coli from foreign gene expression. Though lacking any sequence similarity with E. coli H-NS, Rv3852 was annotated ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00129-17
更新日期:2017-07-25 00:00:00
abstract::A characteristic feature of biofilm formation is the production of a protective extracellular polymeric matrix. In the gram-positive bacterium Bacillus subtilis, the biofilm matrix is synthesized by the products of the epsABCDEFGHIJKLMNO operon (hereafter called the eps operon) and yqxM-sipW-tasA loci. Transcription f...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00618-09
更新日期:2009-11-01 00:00:00
abstract::The ubiquitous opportunistic pathogen Pseudomonas aeruginosa has five aerobic terminal oxidases: bo(3)-type quinol oxidase (Cyo), cyanide-insensitive oxidase (CIO), aa3-type cytochrome c oxidase (aa3), and two cbb(3)-type cytochrome c oxidases (cbb(3)-1and cbb(3)-2). These terminal oxidases are differentially regulate...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02176-14
更新日期:2014-12-01 00:00:00
abstract::The crystal structure of Escherichia coli PdxY, the protein product of the pdxY gene, has been determined to a 2.2-A resolution. PdxY is a member of the ribokinase superfamily of enzymes and has sequence homology with pyridoxal kinases that phosphorylate pyridoxal at the C-5' hydroxyl. The protein is a homodimer with ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.23.8074-8082.2004
更新日期:2004-12-01 00:00:00
abstract::The antiadaptor protein IraD inhibits the proteolysis of the alternative sigma factor, RpoS, which promotes the synthesis of >100 genes during the general stress response and during stationary phase. Our previous results showed that IraD determines RpoS steady-state levels during exponential growth and mediates its st...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00412-09
更新日期:2009-12-01 00:00:00
abstract::The DNA sequence of a 3.23-kilobase fragment of the Escherichia coli chromosome encoding biosynthetic arginine decarboxylase (ADC) was determined. This sequence contained the speA open reading frame (ORF) as well as partial speB and metK ORFs. The ADC ORF is 1,974 nucleotides long; the deduced polypeptide contains 658...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.8.4631-4640.1990
更新日期:1990-08-01 00:00:00
abstract::Gaspar, Andrew J. (Fort Detrick, Frederick, Md.), Hugh B. Tresselt, and Martha K. Ward. New solid medium for enhanced growth of Pasteurella tularensis. J. Bacteriol. 82:564-569. 1961.-The purpose of this work was to develop a solid medium for more rapid growth of Pasteurella tularensis, especially from small inocula c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.82.4.564-569.1961
更新日期:1961-10-01 00:00:00
abstract::The lipopolysaccharide (LPS) from Rhizobium trifolii 0403 was isolated at different stages of growth and was examined for its (i) ability to bind a white clover lectin (trifoliin A), (ii) immunochemical properties, and (iii) composition. There was significantly more binding of trifoliin A to purified LPS and cells in ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.148.2.697-711.1981
更新日期:1981-11-01 00:00:00
abstract::There are two closely related hik31 operons involved in signal transduction on the chromosome and the pSYSX plasmid in the cyanobacterium Synechocystis sp. strain PCC 6803. We studied the growth, cell morphology, and gene expression in operon and hik mutants for both copies, under different growth conditions, to exami...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06207-11
更新日期:2012-01-01 00:00:00
abstract::Pseudomonas aeruginosa hemolytic phospholipase C (PlcH) degrades phosphatidylcholine (PC), an abundant lipid in cell membranes and lung surfactant. A ΔplcHR mutant, known to be defective in virulence in animal models, was less able to colonize epithelial cell monolayers and was defective in biofilm formation on plasti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02169-12
更新日期:2013-07-01 00:00:00
abstract::Cerulenin is a fungal mycotoxin that potently inhibits fatty acid synthesis by covalent modification of the active site thiol of the chain-elongation subtypes of beta-ketoacyl-acyl carrier protein (ACP) synthases. The Bacillus subtilis fabF (yjaY) gene (fabF(b)) encodes an enzyme that catalyzes the condensation of mal...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.10.3032-3040.2001
更新日期:2001-05-01 00:00:00
abstract::Expression of the catalase-peroxidase of Caulobacter crescentus, a gram-negative member of the alpha subdivision of the Proteobacteria, is 50-fold higher in stationary-phase cultures than in exponential cultures. To identify regulators of the starvation response, Tn5 insertion mutants were isolated with reduced expres...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.19.6152-6159.1999
更新日期:1999-10-01 00:00:00
abstract::The stationary phase/general stress response sigma factor RpoS (σ(S)) is necessary for adaptation and restoration of homeostasis in stationary phase. As a physiological consequence, its levels are tightly regulated at least at two levels. Multiple small regulatory RNA molecules modulate its translation, in a manner th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01013-13
更新日期:2014-02-01 00:00:00
abstract::Cultures of a stable L-phase variant of Streptococcus faecium F24 produced and retained peptidoglycan precursors intracellularly over the entire growth cycle in a chemically defined medium. The identity of the most abundant precursor, UDP N-acetylmuramyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine (UDP-MurNAc-penta...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.135.3.900-910.1978
更新日期:1978-09-01 00:00:00
abstract::During bacteriophage T7 infection, the Escherichia coli RNA polymerase beta' subunit is phosphorylated by the phage-encoded kinase Gp0.7. Here, we used proteolytic degradation and mutational analysis to localize the phosphorylation site to a single amino acid, Thr(1068), in the evolutionarily hypervariable segment of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.10.3470-3476.2006
更新日期:2006-05-01 00:00:00
abstract::Cleared lysates of 13 species of filamentous cyanobacteria were examined for the presence of extrachromosomal DNA by using agarose gel electrophoresis and ethidium bromide staining. Seven of the 13 species contained extrachromosomal covalently closed circular DNA, and all but 1 species contained multiple elements. The...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.136.1.414-418.1978
更新日期:1978-10-01 00:00:00
abstract::The structural gene for histidyl-tRNA synthetase was localized to 53.8 min on the Escherichia coli genome. The gene order in this region was determined to be dapE-purC-upp-purG-(guaA, guaB)-hisS-glyA. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.138.1.264-267.1979
更新日期:1979-04-01 00:00:00
abstract::The effects of guanosine tetraphosphate (ppGpp) and pyrimidine ribonucleoside triphosphates on Escherichia coli aspartate transcarbamylase (ATCase) synthesis were examined. To determine the effect of ppGpp, a stringent (relA+) and relaxed (relA) isogenic pair of E. coli K-12 strains was starved for isoleucine, and the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.2.998-1007.1983
更新日期:1983-02-01 00:00:00
abstract::Anthranilate (2-aminobenzoate) is an important intermediate in tryptophan metabolism. In order to investigate the degradation of tryptophan through anthranilate by Burkholderia cepacia, several plasposon mutations were constructed of strain DBO1 and one mutant with the plasposon insertion in the anthranilate dioxygena...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.19.5871-5881.2003
更新日期:2003-10-01 00:00:00
abstract::The proteome of Haemophilus influenzae strain Rd KW20 was analyzed by liquid chromatography (LC) coupled with ion trap tandem mass spectrometry (MS/MS). This approach does not require a gel electrophoresis step and provides a rapidly developed snapshot of the proteome. In order to gain insight into the central metabol...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.15.4593-4602.2003
更新日期:2003-08-01 00:00:00
abstract::Biofilms contribute to virulence of Staphylococcus aureus. Formation of biofilms is multifactorial, involving polysaccharide, protein, and DNA components, which are controlled by various regulators. Here we report that deletion of the rsp gene resulted in an increase in biofilm formation in strain MW2, suggesting that...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05454-11
更新日期:2011-10-01 00:00:00
abstract:UNLABELLED:The σE envelope stress response is an essential signal transduction pathway which detects and removes mistargeted outer membrane (OM) β-barrel proteins (OMPs) in the periplasm of Escherichia coli It relies on σE, an alternative sigma factor encoded by the rpoE gene. Here we report a novel mutation, a nucleot...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00340-16
更新日期:2016-08-11 00:00:00
abstract::The phs mutation of Escherichia coli has been suggested to affect the Na+/H+ antiport (D. Zilberstein, E. Padan, and S. Schuldiner, FEBS Lett. 168:327-330, 1980). We have recently shown that the mutation affects the rpoA gene and thus affects transcription. The extent of the pleiotropy of the phs mutation was investig...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.164.2.904-910.1985
更新日期:1985-11-01 00:00:00
abstract::We have used Tn917lacZ to mutagenize the Bacillus subtilis chromosome and have isolated mutants that are defective in chemotaxis and motility. Mapping of the transposon inserts identified two new loci. Mutations in one of these loci generated mutants that had paralyzed flagella. Accordingly, we designate this a mot lo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.12.6841-6848.1990
更新日期:1990-12-01 00:00:00
abstract::We have isolated and characterized two minor extracellular proteases from culture supernatants of a strain of Bacillus subtilis containing deletion mutations of the genes for the extracellular proteases subtilisin (apr) and neutral protease (npr) and a minor extracellular protease (epr) as well as intracellular serine...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.2.1019-1023.1990
更新日期:1990-02-01 00:00:00
abstract::Alcohol-oxidizing enzymes of the facultative methylotroph PAR were investigated after growth of the bacteria on methanol and ethanol. During methanol growth only a phenazine methosulfate-linked alcohol dehydrogenase was detected. This enzyme had broad specificity for primary alcohols and was also capable of oxidation ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.135.1.251-258.1978
更新日期:1978-07-01 00:00:00
abstract::Benzoyl coenzyme A (benzoyl-CoA) reductase is a key enzyme in the anaerobic metabolism of aromatic compounds catalyzing the ATP-driven reductive dearomatization of benzoyl-CoA. The enzyme from Thauera aromatica uses a reduced 2[4Fe-4S] ferredoxin as electron donor. In this work, we identified 2-oxoglutarate:ferredoxin...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.14.3975-3983.2002
更新日期:2002-07-01 00:00:00
abstract::The phoP-phoQ-regulated pagC locus is essential for full virulence and survival within macrophages of Salmonella typhimurium. The protein product, DNA sequence, and transcript of pagC were determined. The pagC locus encodes a single 188-amino-acid membrane protein that is similar to the ail-encoded eucaryotic cell inv...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.1.86-93.1991
更新日期:1991-01-01 00:00:00
abstract::The WalRK two-component system plays important roles in maintaining cell wall homeostasis and responding to antibiotic stress in low-GC Gram-positive bacteria. In the major human pathogen, Streptococcus pneumoniae, phosphorylated WalR(Spn) (VicR) response regulator positively controls the transcription of genes encodi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01690-09
更新日期:2010-05-01 00:00:00
abstract::Salmonella typhimurium LT2 excreted under certain conditions an antibiotic substance designated typhimuricin. It is suggested that the LT2 "cryptic" plasmid is involved in its production and in the immunity to it. Preliminary characterization of typhimuricin is presented. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:
更新日期:1982-10-01 00:00:00