ftsZ is an essential cell division gene in Escherichia coli.

Abstract:

:The ftsZ gene is thought to be an essential cell division gene in Escherichia coli. We constructed a null allele of ftsZ in a strain carrying additional copies of ftsZ on a plasmid with a temperature-sensitive replication defect. This strain was temperature sensitive for cell division and viability, confirming that ftsZ is an essential cell division gene. Further analysis revealed that after a shift to the nonpermissive temperature, cell division ceased when the level of FtsZ started to decrease, indicating that septation is very sensitive to the level of FtsZ. Subsequent studies showed that nucleoid segregation was normal while FtsZ was decreasing and that ftsZ expression was not autoregulated. The null allele could not be complemented by lambda 16-2, even though this bacteriophage can complement the thermosensitive ftsZ84 mutation and carries 6 kb of DNA upstream of the ftsZ gene.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Dai K,Lutkenhaus J

doi

10.1128/jb.173.11.3500-3506.1991

subject

Has Abstract

pub_date

1991-06-01 00:00:00

pages

3500-6

issue

11

eissn

0021-9193

issn

1098-5530

journal_volume

173

pub_type

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