Effect of growth temperature on folding of carbamoylphosphate synthetases of Salmonella typhimurium and a cold-sensitive derivative.

Abstract:

:The properties of homogeneous preparations of carbamoylphosphate synthetase (CPSase) from wild-type Salmonella typhimurium and a cold-sensitive derivative grown at different growth temperatures were examined. For the cold-sensitive mutant, the affinity for glutamine of the form of CPSase synthesized at 20 degrees C was lower than that of the form of the enzyme synthesized at 37 degrees C, regardless of the assay temperature. Thus, the cold sensitivity of the mutant reflects an effect of temperature on the synthesis of the enzyme rather than the activity of the folded enzyme. The two forms also differed in sensitivities to polyclonal antibodies as well as denaturational enthalpies. The combined results support the hypothesis that carAB mutations conferring cold sensitivity identify amino acid residues that are critical in the folding of CPSase. Quite unexpectedly, certain kinetic properties of cloned parent CPSase were also dependent on the growth temperature, although to a much lesser extent than those of the cold-sensitive mutant. The specific activity of wild-type CPSase synthesized at 15 degrees C was 60% of that synthesized at 37 degrees C. Further, CPSase synthesized at 15 degrees C was less thermostable than the enzyme synthesized at 37 degrees C; the difference in stability (delta G) is estimated to be 4,500 cal mol-1. Thus, variation of temperature within the physiological range for growth influences the folding and consequently the properties of CPSase from wild-type S. typhimurium.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Han BD,Nolan WG,Hopkins HP,Jones RT,Ingraham JL,Abdelal AT

doi

10.1128/jb.172.9.5089-5096.1990

subject

Has Abstract

pub_date

1990-09-01 00:00:00

pages

5089-96

issue

9

eissn

0021-9193

issn

1098-5530

journal_volume

172

pub_type

杂志文章
  • Release of the -galactosidase-synthesizing system from ultraviolet catabolite repression by cyclic 3',5'-adenosine monophosphate, dark repair, photoreactivation, and cold treatment.

    abstract::Recovery from the inhibitory effect of ultraviolet irradiation on the induced synthesis of beta-galactosidase was studied in Escherichia coli B/r. When irradiated cells (520 ergs/mm(2) at 254 nm) were induced and incubated in minimal medium supplemented with Casamino Acids (conditions of catabolite repression), the ab...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.109.1.391-398.1972

    authors: Swenson PA

    更新日期:1972-01-01 00:00:00

  • Functional complementation of the essential gene fabG1 of Mycobacterium tuberculosis by Mycobacterium smegmatis fabG but not Escherichia coli fabG.

    abstract::Mycolic acids are a key component of the mycobacterial cell wall, providing structure and forming a major permeability barrier. In Mycobacterium tuberculosis mycolic acids are synthesized by type I and type II fatty acid synthases. One of the enzymes of the type II system is encoded by fabG1. We demonstrate here that ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01740-06

    authors: Parish T,Roberts G,Laval F,Schaeffer M,Daffé M,Duncan K

    更新日期:2007-05-01 00:00:00

  • Draft genome of a Brazilian avian-pathogenic Escherichia coli strain and in silico characterization of virulence-related genes.

    abstract::Avian-pathogenic Escherichia coli (APEC) strains cause extraintestinal diseases in avian species. Here, we present the draft genome of an APEC strain (SCI-07) from Brazil that was isolated from skin lesions (gelatinous edema) on the head and periorbital tissues of a laying hen with swollen head syndrome. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00394-12

    authors: Rojas TC,Parizzi LP,Tiba MR,Chen L,Pereira GA,Sangal V,Yang J,Yu J,Dias da Silveira W

    更新日期:2012-06-01 00:00:00

  • Regulation of lysine- and lysine-plus-threonine-inhibitable aspartokinases in Bacillus brevis.

    abstract::Further studies on the expression of the two aspartokinase activities in Bacillus bovis are presented. Aspartokinase I (previously shown to be inhibited and repressed by lysine) was found to be repressed by diaminopimelate in the wild-type strain. However, in a mutant unable to convert diaminopimelate to lysine, starv...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.142.2.424-432.1980

    authors: Hitchcock MJ,Hodgson B,Linforth JL

    更新日期:1980-05-01 00:00:00

  • Control of chromosome replication in thymine-requiring strains of Bacillus subtilis 168.

    abstract::Study of the replication pattern of a number of B. subtilis 168 strains under controlled physiological conditions revealed great interstrain variation in control of replication. Replication patterns were calculated from ratios of purA16/leu-8 and purA16/metB5 transformation frequency. The thymine-independent strains a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.123.3.1055-1067.1975

    authors: Gillin FD,Ganesan AT

    更新日期:1975-09-01 00:00:00

  • Identification and characterization of alcR, a gene encoding an AraC-like regulator of alcaligin siderophore biosynthesis and transport in Bordetella pertussis and Bordetella bronchiseptica.

    abstract::A Bordetella bronchiseptica iron transport mutant was isolated following an enrichment procedure based on streptonigrin resistance. The mutant displayed a growth defect on iron-restricted medium containing ferric alcaligin as the sole iron source. In addition to the apparent inability to acquire iron from the sideroph...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.4.862-870.1998

    authors: Beaumont FC,Kang HY,Brickman TJ,Armstrong SK

    更新日期:1998-02-01 00:00:00

  • Identification of a specific membrane-particle-associated DNA sequence in Bacillus subtilis.

    abstract::After the Bacillus subtilis nucleoid was dissected with restriction endonucleases, a specific DNA sequence from the purA region was isolated in a particulate form that probably originated from the cell membrane. Precise definition of the binding region within this sequence was achieved by a novel procedure based on a ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.166.1.38-43.1986

    authors: Sargent MG,Bennett MF

    更新日期:1986-04-01 00:00:00

  • Three new NifA-regulated genes in the Bradyrhizobium japonicum symbiotic gene region discovered by competitive DNA-RNA hybridization.

    abstract::The so-called symbiotic region of the Bradyrhizobium japonicum chromosome (C. Kündig, H. Hennecke, and M. Göttfert, J. Bacteriol. 175:613-622, 1993) was screened for the presence of genes controlled by the nitrogen fixation regulatory protein NifA. Southern blots of restriction enzyme-digested cosmids that represent a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.6.1472-1480.2000

    authors: Nienaber A,Huber A,Göttfert M,Hennecke H,Fischer HM

    更新日期:2000-03-01 00:00:00

  • Efficient turnover of chlorocatechols is essential for growth of Ralstonia eutropha JMP134(pJP4) in 3-chlorobenzoic acid.

    abstract::Ralstonia eutropha JMP134(pJP4) degrades 3-chlorobenzoate (3-CB) by using two not completely isofunctional, pJP4-encoded chlorocatechol degradation gene clusters, tfdC(I)D(I)E(I)F(I) and tfdD(II)C(II)E(II)F(II). Introduction of several copies of each gene cluster into R. eutropha JMP222, which lacks pJP4 and thus accu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.5.1534-1542.2003

    authors: Pérez-Pantoja D,Ledger T,Pieper DH,González B

    更新日期:2003-03-01 00:00:00

  • Replication of the R factor Rts1 in Proteus mirabilis.

    abstract::The replication of the R factor Rts1 in Proteus mirabilis was examined by using the technique of CsCl density-gradient centrifugation. The proportion of Rts1 deoxyribonucleic acid (DNA) relative to the host chromosomal DNA (% R-DNA) was 7% in both exponential and stationary growth phases in Penassay Broth and suppleme...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.109.2.492-498.1972

    authors: Terawaki Y,Rownd R

    更新日期:1972-02-01 00:00:00

  • Iron-dependent cytochrome c1 expression is mediated by the status of heme in Bradyrhizobium japonicum.

    abstract::The heme prosthetic group of heme proteins contains iron, which can be a limiting nutrient. Here, we show that cytochrome c1 protein from Bradyrhizobium japonicum was strongly affected by the iron status, with low expression in cells grown under iron limitation. This control was not affected in mutants encoding the ir...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.15.5084-5089.2005

    authors: Gao T,O'Brian MR

    更新日期:2005-08-01 00:00:00

  • The pbgPE operon in Photorhabdus luminescens is required for pathogenicity and symbiosis.

    abstract::Photorhabdus is a genus of gram-negative Enterobacteriaceae that is pathogenic to insect larvae while also maintaining a mutualistic relationship with nematodes from the family Heterorhabditis, where the bacteria occupy the gut of the infective juvenile (IJ) stage of the nematode. In this study we describe the identif...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.1.77-84.2005

    authors: Bennett HP,Clarke DJ

    更新日期:2005-01-01 00:00:00

  • Expression of ClpB, an analog of the ATP-dependent protease regulatory subunit in Escherichia coli, is controlled by a heat shock sigma factor (sigma 32).

    abstract::Escherichia coli K-12 produces at least two ATP-dependent proteases, Lon (La) and Clp (Ti), the latter consisting of a regulatory subunit (ClpA) and a proteolytic subunit (ClpP). The gene clpB encoding an analog of ClpA had been found at 57 min on the E. coli chromosome. Cloning and examination of novel heat shock pro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.14.4247-4253.1991

    authors: Kitagawa M,Wada C,Yoshioka S,Yura T

    更新日期:1991-07-01 00:00:00

  • Ferric reductase activity in Azotobacter vinelandii and its inhibition by Zn2+.

    abstract::Ferric reductase activity was examined in Azotobacter vinelandii and was found to be located in the cytoplasm. The specific activities of soluble cell extracts were not affected by the iron concentration of the growth medium; however, activity was inhibited by the presence of Zn2+ during cell growth and also by the ad...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.7.4031-4037.1989

    authors: Huyer M,Page WJ

    更新日期:1989-07-01 00:00:00

  • Analysis of plasmid deletional instability in Bacillus subtilis.

    abstract::Using a model system, we have studied deletion formation in Bacillus subtilis. When the staphylococcal plasmids pSA2100 (7.1 kilobases) and pUB110 (4.5 kilobases) were ligated to one another at their unique XbaI sites and transformed into either rec+ or recE4 strains of B. subtilis, an intramolecular recombination eve...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.162.3.1014-1023.1985

    authors: Hahn J,Dubnau D

    更新日期:1985-06-01 00:00:00

  • De novo genome project for the aromatic degrader Rhodococcus pyridinivorans strain AK37.

    abstract::Here, we present the complete genome sequence of Rhodococcus pyridinivorans AK37 strain NCAIM PB1376, which was isolated from an oil-polluted site in Hungary. R. pyridinivorans AK37 is an aerobic, nonsporulating, nonmotile, gram-positive bacterium with remarkable aromatic-decomposing activity. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06603-11

    authors: Kriszt B,Táncsics A,Cserháti M,Tóth Á,Nagy I,Horváth B,Nagy I,Tamura T,Kukolya J,Szoboszlay S

    更新日期:2012-03-01 00:00:00

  • Opines stimulate induction of the vir genes of the Agrobacterium tumefaciens Ti plasmid.

    abstract::Upon incubation of Agrobacterium tumefaciens A348 with acetosyringone, the vir genes encoded by the Ti (tumor-inducing) plasmid are induced. The addition of certain opines, including octopine, nopaline, leucinopine, and succinamopine, enhanced this induction 2- to 10-fold. The compounds mannopine, acetopine, arginine,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.7.3696-3703.1989

    authors: Veluthambi K,Krishnan M,Gould JH,Smith RH,Gelvin SB

    更新日期:1989-07-01 00:00:00

  • Direction of deoxyribonucleic acid replication in Escherichia coli under various conditions of cell growth.

    abstract::The direction of chromosome replication in a temperature-sensitive initiation mutant of Escherichia coli (CT28) is shown autoradiographically to be bidirectional. This mode of replication persists even when the rate of replication is reduced by slow growth in succinate minimal medium or in the presence of chlorampheni...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.125.1.346-352.1976

    authors: Rodriguez RL,Davern CI

    更新日期:1976-01-01 00:00:00

  • Effect of pH on the immunogenicity of Mycoplasma pneumoniae.

    abstract::Mycoplasma pneumoniae harvested from media which had become acid lost the ability both to induce formation of tetrazolium reduction inhibition antibody and to act as antigens in immunodiffusion against human convalescent-phase sera. Incorporation of N-tris(hydroxymethyl)-2-aminoethane sulfonic acid and N-2-hydroxyethy...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.97.2.612-619.1969

    authors: Pollack JD,Somerson NL,Senterfit LB

    更新日期:1969-02-01 00:00:00

  • Isolation of Neurospora crassa bradytrophs.

    abstract::A method was developed for the isolation of Neurospora bradytrophs. The bradytrophs (representing lesions in a number of pathways) were resistant to DL-p-fluorophenylalanine when growing in a leaky fashion but were sensitive when grown in the presence of their stimulating supplement. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.140.3.1133-1136.1979

    authors: Kinsey JA

    更新日期:1979-12-01 00:00:00

  • Novel missense mutations that affect the transport function of MalK, the ATP-binding-cassette subunit of the Salmonella enterica serovar typhimurium maltose transport system.

    abstract::We report on novel mutations in the malK gene of Salmonella enterica serovar Typhimurium, encoding the ATPase subunit of the maltose transporter (MalFGK(2)). Biochemical analysis suggests that (i) L86 might be involved in a signaling step during substrate translocation and (ii) E306 may be critical for the structural ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.5.1432-1436.2000

    authors: Hunke S,Landmesser H,Schneider E

    更新日期:2000-03-01 00:00:00

  • Effects of cycloheximide and puromycin on synthesis of simian virus 40 T antigen in green monkey kidney cells.

    abstract::Gilden, R. V. (Wistar Institute, Philadelphia, Pa.), and R. I. Carp. Effects of cycloheximide and puromycin on synthesis of simian virus 40 T antigen in green monkey kidney cells. J. Bacteriol. 91:1295-1297. 1966.-Synthesis of the simian virus 40 (SV40) T antigen in primary African green monkey kidney cells was abolis...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.91.3.1295-1297.1966

    authors: Gilden RV,Carp RI

    更新日期:1966-03-01 00:00:00

  • Mutations in motif II of Escherichia coli DNA helicase II render the enzyme nonfunctional in both mismatch repair and excision repair with differential effects on the unwinding reaction.

    abstract::Site-directed mutagenesis has been employed to address the functional significance of the highly conserved aspartic and glutamic acid residues present in the Walker B (also called motif II) sequence in Escherichia coli DNA helicase II. Two mutant proteins, UvrDE221Q and UvrDD220NE221Q, were expressed and purified to a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.19.5612-5621.1995

    authors: Brosh RM Jr,Matson SW

    更新日期:1995-10-01 00:00:00

  • Extracellular Electron Transfer: Respiratory or Nutrient Homeostasis?

    abstract::Exoelectrogens are able to transfer electrons extracellularly, enabling them to respire on insoluble terminal electron acceptors. Extensively studied exoelectrogens, such as Geobacter sulfurreducens and Shewanella oneidensis, are Gram negative. More recently, it has been reported that Gram-positive bacteria, such as L...

    journal_title:Journal of bacteriology

    pub_type: 评论,杂志文章

    doi:10.1128/JB.00029-20

    authors: Jeuken LJC,Hards K,Nakatani Y

    更新日期:2020-03-11 00:00:00

  • Characterization of the Campylobacter jejuni heptosyltransferase II gene, waaF, provides genetic evidence that extracellular polysaccharide is lipid A core independent.

    abstract::Campylobacter jejuni produces both lipooligosaccharide (LOS) and a higher-molecular-weight polysaccharide that is believed to form a capsule. The role of these surface polysaccharides in C. jejuni-mediated enteric disease is unclear; however, epitopes associated with the LOS are linked to the development of neurologic...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.8.2100-2107.2002

    authors: Oldfield NJ,Moran AP,Millar LA,Prendergast MM,Ketley JM

    更新日期:2002-04-01 00:00:00

  • Identification of genes encoding exported Mycobacterium tuberculosis proteins using a Tn552'phoA in vitro transposition system.

    abstract::Secreted and cell envelope-associated proteins are important to both Mycobacterium tuberculosis pathogenesis and the generation of protective immunity to M. tuberculosis. We used an in vitro Tn552'phoA transposition system to identify exported proteins of M. tuberculosis. The system is simple and efficient, and the tr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.10.2732-2740.2000

    authors: Braunstein M,Griffin TJ IV,Kriakov JI,Friedman ST,Grindley ND,Jacobs WR Jr

    更新日期:2000-05-01 00:00:00

  • Expression, purification, and characterization of AknX anthrone oxygenase, which is involved in aklavinone biosynthesis in Streptomyces galilaeus.

    abstract::In streptomycete anthracycline biosynthetic gene clusters, small open reading frames are located just upstream of minimal polyketide synthase genes. aknX is such a gene found in the aklavinone-aclacinomycin biosynthetic gene cluster of Streptomyces galilaeus. In order to identify its function, the aknX gene was expres...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.22.6115-6122.2002

    authors: Chung JY,Fujii I,Harada S,Sankawa U,Ebizuka Y

    更新日期:2002-11-01 00:00:00

  • Occurrence of a class II restriction endonuclease in Staphylococcus aureus.

    abstract::The occurrence of class II restriction endonucleases (enzymes that both recognize and cleave a specific nucleotide sequence in deoxyribonucleic acid [DNA] in Staphylococcus aureus has been investigated by analysis of crude extracts obtained from different propagating strains of the International Phage Typing System. O...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.131.2.645-649.1977

    authors: Stobberingh EE,Schiphof R,Sussenbach JS

    更新日期:1977-08-01 00:00:00

  • BACTERIOPHAGE COMMON TO PASTEURELLA PESTIS AND ESCHERICHIA COLI.

    abstract::Hertman, I. (Israel Institute for Biological Research, Ness Ziona, Israel). Bacteriophage common to Pasteurella pestis and Escherichia coli. J. Bacteriol. 88:1002-1005. 1964.-Phage Y of Pasteurella pestis and phage T(3) of Escherichia coli are serologically related and have common bacterial hosts. Anti-Y serum neutral...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.88.4.1002-1005.1964

    authors: HERTMAN I

    更新日期:1964-10-01 00:00:00

  • The A1A0 ATPase from Methanosarcina mazei: cloning of the 5' end of the aha operon encoding the membrane domain and expression of the proteolipid in a membrane-bound form in Escherichia coli.

    abstract::Three additional ATPase genes, clustered in the order ahaH, ahaI, and ahaK, were found upstream of the previously characterized genes ahaECFABDG coding for the archaeal A1A0 ATPase from Methanosarcina mazei. ahaH, the first gene in the cluster, is preceded by a conserved promoter sequence. Northern blot analysis revea...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.13.3448-3452.1998

    authors: Ruppert C,Wimmers S,Lemker T,Müller V

    更新日期:1998-07-01 00:00:00