Abstract:
:The properties of homogeneous preparations of carbamoylphosphate synthetase (CPSase) from wild-type Salmonella typhimurium and a cold-sensitive derivative grown at different growth temperatures were examined. For the cold-sensitive mutant, the affinity for glutamine of the form of CPSase synthesized at 20 degrees C was lower than that of the form of the enzyme synthesized at 37 degrees C, regardless of the assay temperature. Thus, the cold sensitivity of the mutant reflects an effect of temperature on the synthesis of the enzyme rather than the activity of the folded enzyme. The two forms also differed in sensitivities to polyclonal antibodies as well as denaturational enthalpies. The combined results support the hypothesis that carAB mutations conferring cold sensitivity identify amino acid residues that are critical in the folding of CPSase. Quite unexpectedly, certain kinetic properties of cloned parent CPSase were also dependent on the growth temperature, although to a much lesser extent than those of the cold-sensitive mutant. The specific activity of wild-type CPSase synthesized at 15 degrees C was 60% of that synthesized at 37 degrees C. Further, CPSase synthesized at 15 degrees C was less thermostable than the enzyme synthesized at 37 degrees C; the difference in stability (delta G) is estimated to be 4,500 cal mol-1. Thus, variation of temperature within the physiological range for growth influences the folding and consequently the properties of CPSase from wild-type S. typhimurium.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Han BD,Nolan WG,Hopkins HP,Jones RT,Ingraham JL,Abdelal ATdoi
10.1128/jb.172.9.5089-5096.1990subject
Has Abstractpub_date
1990-09-01 00:00:00pages
5089-96issue
9eissn
0021-9193issn
1098-5530journal_volume
172pub_type
杂志文章abstract::Recovery from the inhibitory effect of ultraviolet irradiation on the induced synthesis of beta-galactosidase was studied in Escherichia coli B/r. When irradiated cells (520 ergs/mm(2) at 254 nm) were induced and incubated in minimal medium supplemented with Casamino Acids (conditions of catabolite repression), the ab...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.109.1.391-398.1972
更新日期:1972-01-01 00:00:00
abstract::Mycolic acids are a key component of the mycobacterial cell wall, providing structure and forming a major permeability barrier. In Mycobacterium tuberculosis mycolic acids are synthesized by type I and type II fatty acid synthases. One of the enzymes of the type II system is encoded by fabG1. We demonstrate here that ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01740-06
更新日期:2007-05-01 00:00:00
abstract::Avian-pathogenic Escherichia coli (APEC) strains cause extraintestinal diseases in avian species. Here, we present the draft genome of an APEC strain (SCI-07) from Brazil that was isolated from skin lesions (gelatinous edema) on the head and periorbital tissues of a laying hen with swollen head syndrome. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00394-12
更新日期:2012-06-01 00:00:00
abstract::Further studies on the expression of the two aspartokinase activities in Bacillus bovis are presented. Aspartokinase I (previously shown to be inhibited and repressed by lysine) was found to be repressed by diaminopimelate in the wild-type strain. However, in a mutant unable to convert diaminopimelate to lysine, starv...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.142.2.424-432.1980
更新日期:1980-05-01 00:00:00
abstract::Study of the replication pattern of a number of B. subtilis 168 strains under controlled physiological conditions revealed great interstrain variation in control of replication. Replication patterns were calculated from ratios of purA16/leu-8 and purA16/metB5 transformation frequency. The thymine-independent strains a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.123.3.1055-1067.1975
更新日期:1975-09-01 00:00:00
abstract::A Bordetella bronchiseptica iron transport mutant was isolated following an enrichment procedure based on streptonigrin resistance. The mutant displayed a growth defect on iron-restricted medium containing ferric alcaligin as the sole iron source. In addition to the apparent inability to acquire iron from the sideroph...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.4.862-870.1998
更新日期:1998-02-01 00:00:00
abstract::After the Bacillus subtilis nucleoid was dissected with restriction endonucleases, a specific DNA sequence from the purA region was isolated in a particulate form that probably originated from the cell membrane. Precise definition of the binding region within this sequence was achieved by a novel procedure based on a ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.166.1.38-43.1986
更新日期:1986-04-01 00:00:00
abstract::The so-called symbiotic region of the Bradyrhizobium japonicum chromosome (C. Kündig, H. Hennecke, and M. Göttfert, J. Bacteriol. 175:613-622, 1993) was screened for the presence of genes controlled by the nitrogen fixation regulatory protein NifA. Southern blots of restriction enzyme-digested cosmids that represent a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.6.1472-1480.2000
更新日期:2000-03-01 00:00:00
abstract::Ralstonia eutropha JMP134(pJP4) degrades 3-chlorobenzoate (3-CB) by using two not completely isofunctional, pJP4-encoded chlorocatechol degradation gene clusters, tfdC(I)D(I)E(I)F(I) and tfdD(II)C(II)E(II)F(II). Introduction of several copies of each gene cluster into R. eutropha JMP222, which lacks pJP4 and thus accu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.5.1534-1542.2003
更新日期:2003-03-01 00:00:00
abstract::The replication of the R factor Rts1 in Proteus mirabilis was examined by using the technique of CsCl density-gradient centrifugation. The proportion of Rts1 deoxyribonucleic acid (DNA) relative to the host chromosomal DNA (% R-DNA) was 7% in both exponential and stationary growth phases in Penassay Broth and suppleme...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.109.2.492-498.1972
更新日期:1972-02-01 00:00:00
abstract::The heme prosthetic group of heme proteins contains iron, which can be a limiting nutrient. Here, we show that cytochrome c1 protein from Bradyrhizobium japonicum was strongly affected by the iron status, with low expression in cells grown under iron limitation. This control was not affected in mutants encoding the ir...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.15.5084-5089.2005
更新日期:2005-08-01 00:00:00
abstract::Photorhabdus is a genus of gram-negative Enterobacteriaceae that is pathogenic to insect larvae while also maintaining a mutualistic relationship with nematodes from the family Heterorhabditis, where the bacteria occupy the gut of the infective juvenile (IJ) stage of the nematode. In this study we describe the identif...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.1.77-84.2005
更新日期:2005-01-01 00:00:00
abstract::Escherichia coli K-12 produces at least two ATP-dependent proteases, Lon (La) and Clp (Ti), the latter consisting of a regulatory subunit (ClpA) and a proteolytic subunit (ClpP). The gene clpB encoding an analog of ClpA had been found at 57 min on the E. coli chromosome. Cloning and examination of novel heat shock pro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.14.4247-4253.1991
更新日期:1991-07-01 00:00:00
abstract::Ferric reductase activity was examined in Azotobacter vinelandii and was found to be located in the cytoplasm. The specific activities of soluble cell extracts were not affected by the iron concentration of the growth medium; however, activity was inhibited by the presence of Zn2+ during cell growth and also by the ad...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.7.4031-4037.1989
更新日期:1989-07-01 00:00:00
abstract::Using a model system, we have studied deletion formation in Bacillus subtilis. When the staphylococcal plasmids pSA2100 (7.1 kilobases) and pUB110 (4.5 kilobases) were ligated to one another at their unique XbaI sites and transformed into either rec+ or recE4 strains of B. subtilis, an intramolecular recombination eve...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.162.3.1014-1023.1985
更新日期:1985-06-01 00:00:00
abstract::Here, we present the complete genome sequence of Rhodococcus pyridinivorans AK37 strain NCAIM PB1376, which was isolated from an oil-polluted site in Hungary. R. pyridinivorans AK37 is an aerobic, nonsporulating, nonmotile, gram-positive bacterium with remarkable aromatic-decomposing activity. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06603-11
更新日期:2012-03-01 00:00:00
abstract::Upon incubation of Agrobacterium tumefaciens A348 with acetosyringone, the vir genes encoded by the Ti (tumor-inducing) plasmid are induced. The addition of certain opines, including octopine, nopaline, leucinopine, and succinamopine, enhanced this induction 2- to 10-fold. The compounds mannopine, acetopine, arginine,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.7.3696-3703.1989
更新日期:1989-07-01 00:00:00
abstract::The direction of chromosome replication in a temperature-sensitive initiation mutant of Escherichia coli (CT28) is shown autoradiographically to be bidirectional. This mode of replication persists even when the rate of replication is reduced by slow growth in succinate minimal medium or in the presence of chlorampheni...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.1.346-352.1976
更新日期:1976-01-01 00:00:00
abstract::Mycoplasma pneumoniae harvested from media which had become acid lost the ability both to induce formation of tetrazolium reduction inhibition antibody and to act as antigens in immunodiffusion against human convalescent-phase sera. Incorporation of N-tris(hydroxymethyl)-2-aminoethane sulfonic acid and N-2-hydroxyethy...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.97.2.612-619.1969
更新日期:1969-02-01 00:00:00
abstract::A method was developed for the isolation of Neurospora bradytrophs. The bradytrophs (representing lesions in a number of pathways) were resistant to DL-p-fluorophenylalanine when growing in a leaky fashion but were sensitive when grown in the presence of their stimulating supplement. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.140.3.1133-1136.1979
更新日期:1979-12-01 00:00:00
abstract::We report on novel mutations in the malK gene of Salmonella enterica serovar Typhimurium, encoding the ATPase subunit of the maltose transporter (MalFGK(2)). Biochemical analysis suggests that (i) L86 might be involved in a signaling step during substrate translocation and (ii) E306 may be critical for the structural ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.5.1432-1436.2000
更新日期:2000-03-01 00:00:00
abstract::Gilden, R. V. (Wistar Institute, Philadelphia, Pa.), and R. I. Carp. Effects of cycloheximide and puromycin on synthesis of simian virus 40 T antigen in green monkey kidney cells. J. Bacteriol. 91:1295-1297. 1966.-Synthesis of the simian virus 40 (SV40) T antigen in primary African green monkey kidney cells was abolis...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.3.1295-1297.1966
更新日期:1966-03-01 00:00:00
abstract::Site-directed mutagenesis has been employed to address the functional significance of the highly conserved aspartic and glutamic acid residues present in the Walker B (also called motif II) sequence in Escherichia coli DNA helicase II. Two mutant proteins, UvrDE221Q and UvrDD220NE221Q, were expressed and purified to a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.19.5612-5621.1995
更新日期:1995-10-01 00:00:00
abstract::Exoelectrogens are able to transfer electrons extracellularly, enabling them to respire on insoluble terminal electron acceptors. Extensively studied exoelectrogens, such as Geobacter sulfurreducens and Shewanella oneidensis, are Gram negative. More recently, it has been reported that Gram-positive bacteria, such as L...
journal_title:Journal of bacteriology
pub_type: 评论,杂志文章
doi:10.1128/JB.00029-20
更新日期:2020-03-11 00:00:00
abstract::Campylobacter jejuni produces both lipooligosaccharide (LOS) and a higher-molecular-weight polysaccharide that is believed to form a capsule. The role of these surface polysaccharides in C. jejuni-mediated enteric disease is unclear; however, epitopes associated with the LOS are linked to the development of neurologic...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.8.2100-2107.2002
更新日期:2002-04-01 00:00:00
abstract::Secreted and cell envelope-associated proteins are important to both Mycobacterium tuberculosis pathogenesis and the generation of protective immunity to M. tuberculosis. We used an in vitro Tn552'phoA transposition system to identify exported proteins of M. tuberculosis. The system is simple and efficient, and the tr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.10.2732-2740.2000
更新日期:2000-05-01 00:00:00
abstract::In streptomycete anthracycline biosynthetic gene clusters, small open reading frames are located just upstream of minimal polyketide synthase genes. aknX is such a gene found in the aklavinone-aclacinomycin biosynthetic gene cluster of Streptomyces galilaeus. In order to identify its function, the aknX gene was expres...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.22.6115-6122.2002
更新日期:2002-11-01 00:00:00
abstract::The occurrence of class II restriction endonucleases (enzymes that both recognize and cleave a specific nucleotide sequence in deoxyribonucleic acid [DNA] in Staphylococcus aureus has been investigated by analysis of crude extracts obtained from different propagating strains of the International Phage Typing System. O...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.131.2.645-649.1977
更新日期:1977-08-01 00:00:00
abstract::Hertman, I. (Israel Institute for Biological Research, Ness Ziona, Israel). Bacteriophage common to Pasteurella pestis and Escherichia coli. J. Bacteriol. 88:1002-1005. 1964.-Phage Y of Pasteurella pestis and phage T(3) of Escherichia coli are serologically related and have common bacterial hosts. Anti-Y serum neutral...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.4.1002-1005.1964
更新日期:1964-10-01 00:00:00
abstract::Three additional ATPase genes, clustered in the order ahaH, ahaI, and ahaK, were found upstream of the previously characterized genes ahaECFABDG coding for the archaeal A1A0 ATPase from Methanosarcina mazei. ahaH, the first gene in the cluster, is preceded by a conserved promoter sequence. Northern blot analysis revea...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.13.3448-3452.1998
更新日期:1998-07-01 00:00:00