Occurrence of a class II restriction endonuclease in Staphylococcus aureus.

abstract：:A nicotinamide adenine dinucleotide-specific L-(+)-lactate dehydrogenase (LDH) (EC 1.11.27) from Actinomyces viscosus T-6-1600 was purified approximately 110-fold by a combination of diethylaminoethyl-cellulose and 0.5 M Agarose A column chromatography. The ldh was stable at 26 C, but was quite labile at temperatures ...

journal_title：Journal of bacteriology

authors： Brown AT,Christian CP,Eifert RL

更新日期：1975-06-01 00:00:00

abstract：:Normal animal sera inhibit at least one Clostridium histolyticum proteinase. An assay procedure based on immune hemolysis was developed for the estimation of this inhibition. This inhibitory activity occurs in various levels in the sera of different animal species. The highest titers have been obtained with rat sera. ...

journal_title：Journal of bacteriology

authors： Luzzati A,Goldlust MB,Levine L

更新日期：1968-12-01 00:00:00

abstract：:The effect of adding hemin to anaerobically grown cells of a strain of Staphylococcus epidermidis, which was heme-deficient due to anaerobic growth, has been examined. Cells grown anaerobically in media containing hemin exhibited a marked increase in several oxidative activities as compared with cells grown anaerobica...

journal_title：Journal of bacteriology

authors： Jacobs NJ,Maclosky ER,Conti SF

更新日期：1967-01-01 00:00:00

abstract：:Erwinia chrysanthemi 3937 secretes several pectinolytic enzymes, among which eight isoenzymes of pectate lyases with an endo-cleaving mode (PelA, PelB, PelC, PelD, PelE, PelI, PelL, and PelZ) have been identified. Two exo-cleaving enzymes, the exopolygalacturonate lyase, PelX, and an exo-poly-alpha-D-galacturonosidase...

journal_title：Journal of bacteriology

authors： Shevchik VE,Kester HC,Benen JA,Visser J,Robert-Baudouy J,Hugouvieux-Cotte-Pattat N

更新日期：1999-03-01 00:00:00

abstract：:Four genes, dra, tpp, drm, and pup, that specify enzymes involved in the catabolism of nucleosides and deoxynucleosides in Escherichia coli are known to be very closely linked in the order dra-tpp-drm-pup. By infecting cells with the phage Mu-1 and isolating low-thymine-requiring derivatives of a strain lacking thymid...

journal_title：Journal of bacteriology

authors： Buxton RS

更新日期：1975-02-01 00:00:00

abstract：:RpoS, the sigma factor of enteric bacteria that responds to stress and stationary phase, is subject to complex regulation acting at multiple levels, including transcription, translation, and proteolysis. Increased translation of rpoS mRNA during growth at low temperature, after osmotic challenge, or with a constitutiv...

journal_title：Journal of bacteriology

authors： Jones AM,Goodwill AG,Elliott T

更新日期：2006-07-01 00:00:00

abstract：:The lipoteichoic acids were isolated from phenol extracts of four Listeria strains representing serotypes 4a, 4b, 6a, and 6 to compare the differences in structure of amphiphilic polysaccharides from various serotypes of Listeria spp. The lipoteichoic acids from the four strains examined had the same structure in both...

journal_title：Journal of bacteriology

authors： Uchikawa K,Sekikawa I,Azuma I

更新日期：1986-10-01 00:00:00

abstract：:Archaea are abundant and drive critical microbial processes in the Earth's cold biosphere. Despite this, not enough is known about the molecular mechanisms of cold adaptation and no biochemical studies have been performed on stenopsychrophilic archaea (e.g., Methanogenium frigidum). This study examined the structural ...

journal_title：Journal of bacteriology

authors： Giaquinto L,Curmi PM,Siddiqui KS,Poljak A,DeLong E,DasSarma S,Cavicchioli R

更新日期：2007-08-01 00:00:00

abstract：:wbdA is a mannosyltransferase gene that is involved in synthesis of the Escherichia coli O9a polysaccharide, a mannose homopolymer with a repeating unit of 2-alphaMan-1,2-alphaMan-1,3-alphaMan-1, 3-alphaMan-1. The equivalent structural O polysaccharide in the E. coli O9 and Klebsiella O3 strains is 2-alphaMan-1,2-alph...

journal_title：Journal of bacteriology

authors： Kido N,Kobayashi H

更新日期：2000-05-01 00:00:00

abstract：:Twelve different dominant negative mutants of the Escherichia coli nucleoid-associated protein, H-NS, have been selected and characterized in vivo. The mutants are all severely defective in promoter repression activity in a strain lacking H-NS, and they all disrupt the repression normally exerted by H-NS at two of its...

journal_title：Journal of bacteriology

authors： Williams RM,Rimsky S,Buc H

更新日期：1996-08-01 00:00:00

abstract：:During spore formation, Bacillus subtilis divides asymmetrically, resulting in two cells with different fates. Immediately after division, the transcription factor σ(F) becomes active in the smaller prespore, followed by activation of σ(E) in the larger mother cell. We recently showed that a delay in σ(E) activation r...

journal_title：Journal of bacteriology

authors： Xenopoulos P,Piggot PJ

更新日期：2011-06-01 00:00:00

abstract：:Auxotrophic mutants of the filamentous cyanobacterium Anabaena variabilis were isolated by a method in which, after mutagenesis and before penicllin enrichment, mutant and wild-type cells were separated by cavitation. Auxotrophs were identified by their inability to grow on minimal medium, and they were partially char...

journal_title：Journal of bacteriology

authors： Currier TC,Haury JF,Wolk CP

更新日期：1977-03-01 00:00:00

abstract：:When Agrobacterium tumefaciens cells grown in the presence of tritiated thymidine to label specifically the bacterial deoxyribonucleic acid (DNA) are incubated with carrot root tissue for short periods of time, an appreciable fraction of the label becomes firmly associated with the root tissue. Such association is not...

journal_title：Journal of bacteriology

authors： Yajko DM,Hegeman GD

更新日期：1971-12-01 00:00:00

abstract：:The purine regulon repressor, PurR, was identified as a component of the Escherichia coli regulatory system for pyrC, the gene that encodes dihydroorotase, an enzyme in de novo pyrimidine nucleotide synthesis. PurR binds to a pyrC control site that resembles a pur regulon operator and represses expression by twofold. ...

journal_title：Journal of bacteriology

authors： Choi KY,Zalkin H

更新日期：1990-06-01 00:00:00

abstract：:The syrA and syrB genes involved in syringomycin production in Pseudomonas syringae pv. syringae B301D were identified from an EcoRI-pLAFR3 cosmid library and then physically and functionally analyzed in relation to plant pathogenicity. Homologous recombination of the genes required for syringomycin production from co...

journal_title：Journal of bacteriology

authors： Xu GW,Gross DC

更新日期：1988-12-01 00:00:00

abstract：:The nitrogen assimilation control protein (NAC) binds to a site within the promoter region of the histidine utilization operon (hutUH) of Klebsiella aerogenes, and NAC bound at this site activates transcription of hutUH. This NAC-binding site was characterized by a combination of random and directed DNA mutagenesis. M...

journal_title：Journal of bacteriology

authors： Pomposiello PJ,Janes BK,Bender RA

更新日期：1998-02-01 00:00:00

abstract：:A genetic approach was undertaken to identify normal bacterial genes whose products function to limit the effective concentration of antibiotics. In this approach, a multicopy plasmid library containing cloned Escherichia coli chromosomal sequences was screened for transformants that showed increased resistance to a n...

journal_title：Journal of bacteriology

authors： Gambino L,Gracheck SJ,Miller PF

更新日期：1993-05-01 00:00:00

abstract：:The Bacillus subtilis dciA operon encodes a dipeptide transport complex that is induced rapidly as cells enter stationary phase and initiate sporulation. Expression of this operon in growing cells is repressed by glucose, by a mixture of amino acids, and by the AbrB protein. A genetic screen was devised to identify mu...

journal_title：Journal of bacteriology

authors： Slack FJ,Mueller JP,Sonenshein AL

更新日期：1993-08-01 00:00:00

abstract：:Studies were made of the synthesis of Lac messenger ribonucleic acid (mRNA) by Escherichia coli in the absence of protein synthesis and of the coupling of transcription of lac operon to translation. Lac mRNA was not synthesized in the presence of chloramphenicol, and its synthesis steadily decreased during K(+) depriv...

journal_title：Journal of bacteriology

authors： Artman M,Ennis HL

更新日期：1972-05-01 00:00:00

abstract：:From the cell envelope preparation of Sphingomonas paucimobilis two membrane fractions with different densities were separated by sucrose density gradient ultracentrifugation. The high-density fraction contained several major proteins, phospholipids, and glycosphingolipids, which are the only glycolipids of this lipop...

journal_title：Journal of bacteriology

authors： Kawasaki S,Moriguchi R,Sekiya K,Nakai T,Ono E,Kume K,Kawahara K

更新日期：1994-01-01 00:00:00

abstract：:Three genes were shown to provide functions specific for ferric enterobactin transport in Escherichia coli: fepA encoded the outer membrane receptor, fepB produced a periplasmic protein, and the fepC product was presumably a component of a cytoplasmic membrane permease system for this siderophore. A 10.6-kilobase-pair...

journal_title：Journal of bacteriology

authors： Ozenberger BA,Nahlik MS,McIntosh MA

更新日期：1987-08-01 00:00:00

abstract：:Studies on the composition of total fatty acids of Alcaligenes faecalis harvested at different growth phases have been carried out. Ability of the organism to desaturate palmitic and stearic acid has also been tested. The organism contained palmitic (16:0), stearic (18:0), palmitoleic (16:1), cis-vaccenic (18:1), cycl...

journal_title：Journal of bacteriology

authors： Ghaneker AS,Nair PM

更新日期：1973-05-01 00:00:00

abstract：:Streptococcus pyogenes (group A streptococcus [GAS]), a catalase-negative gram-positive bacterium, is aerotolerant and survives H2O2 exposures that kill many catalase-positive bacteria. The molecular basis of the H2O2 resistance is poorly known. Here, we demonstrate that serotype M49 GAS lacking the Rgg regulator is m...

journal_title：Journal of bacteriology

authors： Pulliainen AT,Hytönen J,Haataja S,Finne J

更新日期：2008-05-01 00:00:00

abstract：:Streptococcus mutans transports glucose via the phosphoenolpyruvate (PEP)-dependent sugar phosphotransferase system (PTS). Earlier studies indicated that an alternate glucose transport system functions in this organism under conditions of high growth rates, low pH, or excess glucose. To identify this system, S. mutans...

journal_title：Journal of bacteriology

authors： Cvitkovitch DG,Boyd DA,Thevenot T,Hamilton IR

更新日期：1995-05-01 00:00:00

abstract：:The steps in the biosynthetic transformation of GTP to 7,8-dihydro-D-erythro-neopterin (H2neopterin), the precursor to the modified folates found in the methanogenic archaea, has been elucidated for the first time in two members of the domain Archaea. In Methanococcus thermophila and Methanobacterium thermoautotrophic...

journal_title：Journal of bacteriology

authors： Howell DM,White RH

更新日期：1997-08-01 00:00:00

abstract：:The cytochrome c(3) of Desulfovibrio desulfuricans and that of D. vulgaris were purified to homogeneity as judged by disc gel electrophoresis and by ultracentrifugation. Both cytochromes had an oxidation-reduction potential of -205 +/- 5 mv at pH 7.0 and showed characteristic absorption bands at 525 and 553 nm in the ...

journal_title：Journal of bacteriology

authors： Drucker H,Campbell LL

更新日期：1969-10-01 00:00:00

abstract：:In Bacillus subtilis, extracellular peptide signaling regulates several biological processes. Secreted Phr signaling peptides are imported into the cell and act intracellularly to antagonize the activity of regulators known as Rap proteins. B. subtilis encodes several Rap proteins and Phr peptides, and the processes r...

journal_title：Journal of bacteriology

authors： Auchtung JM,Lee CA,Grossman AD

更新日期：2006-07-01 00:00:00

abstract：:An m(k) (-)r(k) (-) mutation exerted no measurable effect on 5-bromouracil incorporation by a thymineless derivative of Escherichia coli K. ...

journal_title：Journal of bacteriology

authors： Matney TS,MacDougall NL,Butler MA,Suit JC

更新日期：1970-10-01 00:00:00

abstract：:The pdxK and pdxY genes have been found to code for pyridoxal kinases, enzymes involved in the pyridoxal phosphate salvage pathway. Two pyridoxal kinase structures have recently been published, including Escherichia coli pyridoxal kinase 2 (ePL kinase 2) and sheep pyridoxal kinase, products of the pdxY and pdxK genes,...

journal_title：Journal of bacteriology

authors： Safo MK,Musayev FN,di Salvo ML,Hunt S,Claude JB,Schirch V

更新日期：2006-06-01 00:00:00

abstract：:Acyl carrier protein was localized by immunoelectron microscopy in the cytoplasm of Escherichia coli. These data are inconsistent with the previous report of an association between acyl carrier protein and the inner membrane (H. Van den Bosch, J.R. Williamson, and P.R. Vagelos, Nature [London] 228:338-341, 1970). More...

journal_title：Journal of bacteriology

authors： Jackowski S,Edwards HH,Davis D,Rock CO

更新日期：1985-04-01 00:00:00