Effect of pH on the immunogenicity of Mycoplasma pneumoniae.

Abstract:

:Mycoplasma pneumoniae harvested from media which had become acid lost the ability both to induce formation of tetrazolium reduction inhibition antibody and to act as antigens in immunodiffusion against human convalescent-phase sera. Incorporation of N-tris(hydroxymethyl)-2-aminoethane sulfonic acid and N-2-hydroxyethylpiperazine-N'-2-ethane sulfonic acid buffers into a new medium containing PPLO Serum Fraction instead of horse serum delayed the pH decline. Tris(hydroxymethyl)aminomethane, triethanolamine, and 3,6-endomethylene-1,2,3,6-tetrahydrophthalic acid buffers inhibited growth. Mycoplasmas obtained from buffered cultures retained antigenicity as measured by immunodiffusion and could stimulate tetrazolium reduction inhibition antibody formation in animals.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Pollack JD,Somerson NL,Senterfit LB

doi

10.1128/JB.97.2.612-619.1969

subject

Has Abstract

pub_date

1969-02-01 00:00:00

pages

612-9

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

97

pub_type

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