Contribution of the FtsQ transmembrane segment to localization to the cell division site.

Abstract:

:The Escherichia coli cell division protein FtsQ is a central component of the divisome. FtsQ is a bitopic membrane protein with a large C-terminal periplasmic domain. In this work we investigated the role of the transmembrane segment (TMS) that anchors FtsQ in the cytoplasmic membrane. A set of TMS mutants was made and analyzed for the ability to complement an ftsQ mutant. Study of the various steps involved in FtsQ biogenesis revealed that one mutant (L29/32R;V38P) failed to functionally insert into the membrane, whereas another mutant (L29/32R) was correctly assembled and interacted with FtsB and FtsL but failed to localize efficiently to the cell division site. Our results indicate that the FtsQ TMS plays a role in FtsQ localization to the division site.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Scheffers DJ,Robichon C,Haan GJ,den Blaauwen T,Koningstein G,van Bloois E,Beckwith J,Luirink J

doi

10.1128/JB.00723-07

subject

Has Abstract

pub_date

2007-10-01 00:00:00

pages

7273-80

issue

20

eissn

0021-9193

issn

1098-5530

pii

JB.00723-07

journal_volume

189

pub_type

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