Transcription from fusion promoters generated during transposition of transposon Tn4652 is positively affected by integration host factor in Pseudomonas putida.

Abstract:

:We have previously shown that both ends of the Tn3 family transposon Tn4652 contain integration host factor (IHF) binding sites and that IHF positively regulates expression of the Tn4652 transposase gene tnpA in Pseudomonas putida (R. Hõrak, and M. Kivisaar, J. Bacteriol. 180:2822-2829, 1998). Tn4652 can activate silent genes by creating fusion promoters during the transposition. The promoters are created as fusions between the -35 hexamer provided by the terminal inverted repeats of Tn4652 and the -10 hexamers in the target DNA. Two fusion promoters, PRA1 and PLA1, that contain sequences of the right and left termini of Tn4652, respectively, were chosen for the study of mechanisms of transcription activation. Gel mobility shift analysis using crude extracts from P. putida cells allowed us to detect specific binding of P. putida IHF to the ends of the transposon Tn4652. We found that the rate of transcription from the fusion promoter PRA1 is enhanced by IHF. Notably, the positive effect of IHF on transcription from the promoter PRA1 appeared only when cells of P. putida reached the stationary growth phase. We speculate that the intracellular concentration of IHF might be critical for the in vivo effect of IHF on transcription from the fusion promoters in P. putida. In the case of PLA1, the mechanism of transcription modulation by IHF is different than that observed for PRA1. Our results demonstrate that transcription of neighboring genes from outwardly directed promoters at the ends of a mobile DNA element could be influenced by the same factors that control transposition of the element.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Teras R,Hõrak R,Kivisaar M

doi

10.1128/jb.182.3.589-598.2000

keywords:

subject

Has Abstract

pub_date

2000-02-01 00:00:00

pages

589-98

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

182

pub_type

杂志文章
  • Biofilm formation and dispersal under the influence of the global regulator CsrA of Escherichia coli.

    abstract::The predominant mode of growth of bacteria in the environment is within sessile, matrix-enclosed communities known as biofilms. Biofilms often complicate chronic and difficult-to-treat infections by protecting bacteria from the immune system, decreasing antibiotic efficacy, and dispersing planktonic cells to distant b...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.1.290-301.2002

    authors: Jackson DW,Suzuki K,Oakford L,Simecka JW,Hart ME,Romeo T

    更新日期:2002-01-01 00:00:00

  • Identification and function of the pdxY gene, which encodes a novel pyridoxal kinase involved in the salvage pathway of pyridoxal 5'-phosphate biosynthesis in Escherichia coli K-12.

    abstract::pdrK encodes a pyridoxine (PN)/pyridoxal (PL)/pyridoxamine (PM) kinase thought to function in the salvage pathway of pyridoxal 5'-phosphate (PLP) coenzyme biosynthesis. The observation that pdxK null mutants still contain PL kinase activity led to the hypothesis that Escherichia coli K-12 contains at least one other B...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.7.1814-1821.1998

    authors: Yang Y,Tsui HC,Man TK,Winkler ME

    更新日期:1998-04-01 00:00:00

  • Isolation and characterization of three autolytic enzymes associated with sporulation of Bacillus thuringiensis var. thuringiensis.

    abstract::Cells of Bacillus thuringiensis containing refractile spores autolyzed readily when suspended in buffer. The autolysate contained enzymes which lysed vegetative cell walls of the organism. Three enzymes were isolated from the autolysate, and each was purified approximately 30-fold. One enzyme, most active near pH 4.0,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.96.3.629-638.1968

    authors: Kingan SL,Ensign JC

    更新日期:1968-09-01 00:00:00

  • Diversity among clinical isolates of Histoplasma capsulatum detected by polymerase chain reaction with arbitrary primers.

    abstract::Clinical isolates of the fungal respiratory and systemic pathogen Histoplasma capsulatum have been placed in several different classes by using genomic restriction fragment length polymorphisms (RFLPs), but in general have not been distinguished further. We report here that a polymerase chain reaction (PCR)-based DNA ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.22.7075-7079.1992

    authors: Kersulyte D,Woods JP,Keath EJ,Goldman WE,Berg DE

    更新日期:1992-11-01 00:00:00

  • The Pseudomonas stutzeri-Specific Regulatory Noncoding RNA NfiS Targets katB mRNA Encoding a Catalase Essential for Optimal Oxidative Resistance and Nitrogenase Activity.

    abstract::Pseudomonas stutzeri A1501 is a versatile nitrogen-fixing bacterium capable of living in diverse environments and coping with various oxidative stresses. NfiS, a regulatory noncoding RNA (ncRNA) involved in the control of nitrogen fixation in A1501, was previously shown to be required for optimal resistance to H2O2; h...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00334-19

    authors: Zhang H,Zhan Y,Yan Y,Liu Y,Hu G,Wang S,Yang H,Qiu X,Liu Y,Li J,Lu W,Elmerich C,Lin M

    更新日期:2019-09-06 00:00:00

  • Roles of fimB and fimE in site-specific DNA inversion associated with phase variation of type 1 fimbriae in Escherichia coli.

    abstract::Evidence obtained with an improved in vivo assay of fimbrial phase variation in Escherichia coli supported a revised understanding of the roles of fimB and fimE in the site-specific DNA rearrangement with which they are associated. A previously proposed model argued that fimB and fimE play antagonistic, unidirectional...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.17.5308-5314.1991

    authors: McClain MS,Blomfield IC,Eisenstein BI

    更新日期:1991-09-01 00:00:00

  • SAL-TOL in vivo recombinant plasmid pKF439.

    abstract::SAL-TOL in vivo recombinant plasmid pKF439 was characterized in a strain from a mixed culture of bacteria harboring various degradative plasmids. Analysis of the gene organization of pKF439 revealed that the 57-kilobase TOL fragment, including the 40-kilobase TOL metabolic region, was inserted into the complete SAL re...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.162.3.1325-1328.1985

    authors: Furukawa K,Miyazaki T,Tomizuka N

    更新日期:1985-06-01 00:00:00

  • Positive selection acting on a surface membrane protein of the plant-pathogenic phytoplasmas.

    abstract::Phytoplasmas are plant-pathogenic bacteria that cause numerous diseases. This study shows a strong positive selection on the phytoplasma antigenic membrane protein (Amp). The ratio of nonsynonymous to synonymous substitutions was >1 with all the methods we tested. The clear positive selections imply an important biolo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.9.3424-3428.2006

    authors: Kakizawa S,Oshima K,Jung HY,Suzuki S,Nishigawa H,Arashida R,Miyata S,Ugaki M,Kishino H,Namba S

    更新日期:2006-05-01 00:00:00

  • Stabilization of the pertussis toxin secretion apparatus by the C terminus of PtlD.

    abstract::Pertussis toxin (PT) is secreted from Bordetella pertussis by a type IV secretion system, known as the Ptl transporter, that comprises nine different proteins, PtlA to PtlI. In this study, we found that PtlD is required for the stability of three Ptl proteins, PtlE, PtlF, and PtlH. A region limited to the C-terminal 7...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01106-08

    authors: Verma A,Cheung AM,Burns DL

    更新日期:2008-11-01 00:00:00

  • Clusters of charged residues at the C terminus of MotA and N terminus of MotB are important for function of the Escherichia coli flagellar motor.

    abstract::MotA contains a conserved C-terminal cluster of negatively charged residues, and MotB contains a conserved N-terminal cluster of positively charged residues. Charge-altering mutations affecting these residues impair motility but do not diminish Mot protein levels. The motility defects are reversed by second-site mutat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00407-08

    authors: Hosking ER,Manson MD

    更新日期:2008-08-01 00:00:00

  • Control of cell growth in bacteria: experiments with thymine starvation.

    abstract::When cells of Escherichia coli THU were starved for thymine, they continued to grow without division for at least two successive volume doublings at their initial rate. Within experimental error this average rate of volume increase, 0.21 mum(3) per hr, was identical with that observed in control cultures during two ge...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.105.2.472-476.1971

    authors: Kubitschek HE

    更新日期:1971-02-01 00:00:00

  • Identification by genetic suppression of Escherichia coli TolB residues important for TolB-Pal interaction.

    abstract::The Tol-Pal system of Escherichia coli is involved in maintaining outer membrane stability. Mutations in tolQ, tolR, tolA, tolB, or pal genes result in sensitivity to bile salts and the leakage of periplasmic proteins. Moreover, some of the tol genes are necessary for the entry of group A colicins and the DNA of filam...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.3.821-824.2000

    authors: Ray MC,Germon P,Vianney A,Portalier R,Lazzaroni JC

    更新日期:2000-02-01 00:00:00

  • The requirement for pneumococcal MreC and MreD is relieved by inactivation of the gene encoding PBP1a.

    abstract::MreC and MreD, along with the actin homologue MreB, are required to maintain the shape of rod-shaped bacteria. The depletion of MreCD in rod-shaped bacteria leads to the formation of spherical cells and the accumulation of suppressor mutations. Ovococcus bacteria, such as Streptococcus pneumoniae, lack MreB homologues...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05245-11

    authors: Land AD,Winkler ME

    更新日期:2011-08-01 00:00:00

  • Identification of a ligand on the Wip1 bacteriophage highly specific for a receptor on Bacillus anthracis.

    abstract::Tectiviridae is a family of tailless bacteriophages with Gram-negative and Gram-positive hosts. The family model PRD1 and its close relatives all infect a broad range of enterobacteria by recognizing a plasmid-encoded conjugal transfer complex as a receptor. In contrast, tectiviruses with Gram-positive hosts are highl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00655-13

    authors: Kan S,Fornelos N,Schuch R,Fischetti VA

    更新日期:2013-10-01 00:00:00

  • Mechanism of ToxT-dependent transcriptional activation at the Vibrio cholerae tcpA promoter.

    abstract::The AraC homolog ToxT coordinately regulates virulence gene expression in Vibrio cholerae. ToxT is required for transcriptional activation of the genes encoding cholera toxin and the toxin coregulated pilus, among others. In this work we focused on the interaction of ToxT with the tcpA promoter and investigated the me...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.20.5533-5544.2002

    authors: Hulbert RR,Taylor RK

    更新日期:2002-10-01 00:00:00

  • Lytic effects of staphylococcal alpha-toxin and delta-hemolysin.

    abstract::Several preparations of staphylococcal alpha-toxin and delta-lysin were studied in order to compare hemolytic activity with capacity to lyse bacterial protoplasts. delta-Lysin in relatively low concentration lysed protoplasts of Sarcina lutea, protoplasts of Streptococcus faecalis, and spheroplasts of Escherichia coli...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.96.2.487-491.1968

    authors: Bernheimer AW,Avigad LS,Grushoff P

    更新日期:1968-08-01 00:00:00

  • Circular and linear plasmids of Lyme disease spirochetes have extensive homology: characterization of a repeated DNA element.

    abstract::We have cloned three copies of a repeated DNA segment from Borrelia burgdorferi sensu stricto strain B31, present on both circular and linear plasmids of this and other B. burgdorferi sensu lato strains. The DNA sequences are characterized by a highly homologous segment containing two open reading frames (ORFs), ORF-A...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.8.2287-2298.1996

    authors: Zückert WR,Meyer J

    更新日期:1996-04-01 00:00:00

  • Stabilization of the relaxosome and stimulation of conjugal transfer are genetically distinct functions of the R1162 protein MobB.

    abstract::MobB is a small protein encoded by the broad-host-range plasmid R1162 and required for efficient mobilization of its DNA during conjugation. The protein was shown previously to stabilize the relaxosome, the complex of plasmid DNA and mobilization proteins at the origin of transfer (oriT). We have generated in-frame mo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.7.2124-2131.1999

    authors: Perwez T,Meyer RJ

    更新日期:1999-04-01 00:00:00

  • Phenotypic suppression of phosphofructokinase mutations in Escherichia coli by constitutive expression of the glyoxylate shunt.

    abstract::Fructose-6-phosphate kinase (pfkA) mutants have impaired growth on carbon sources which enter glycolysis at or above the level of fructose-6-phosphate, but the degree of impairment depends on the carbon source (e.g., growth on glucose is very much slower than growth on glucose-6-phosphate). The present report contains...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.118.3.1090-1100.1974

    authors: Vinopal RT,Fraenkel DG

    更新日期:1974-06-01 00:00:00

  • Evolution of Staphylococcus aureus by large chromosomal replacements.

    abstract::Conjugative transfer and replacement of hundreds of kilobases of a bacterial chromosome can occur in vitro, but replacements in nature are either an order of magnitude smaller or involve the movement of mobile genetic elements. We discovered that two lineages of Staphylococcus aureus, including a pandemic methicillin-...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.186.4.1060-1064.2004

    authors: Robinson DA,Enright MC

    更新日期:2004-02-01 00:00:00

  • Antibodies against Staphylococcus aureus in nonimmunized rabbits.

    abstract::Cohen, Jay O. (Communicable Disease Center, Atlanta, Ga.), Glenda S. Cowart, and William B. Cherry. Antibodies against Staphylococcus aureus in nonimmunized rabbits. J. Bacteriol. 82:110-114. 1961.-Antibody against staphylococci was demonstrated in the serum of each of 36 nonimmunized specific-pathogen-free rabbits th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.82.1.110-114.1961

    authors: COHEN JO,COWART GS,CHERRY WB

    更新日期:1961-07-01 00:00:00

  • DRS is far less divergent than streptococcal inhibitor of complement of group A streptococcus.

    abstract::When 100 group A streptococcus isolates were screened, drs, a variant of sic, was identified in emm12 and emm55 isolates. Molecular characterization showed that the drs gene sequence is highly conserved, unlike the sic gene sequence. However, the variation in gene size observed was due to the presence of extra interna...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01619-06

    authors: Sagar V,Kumar R,Ganguly NK,Menon T,Chakraborti A

    更新日期:2007-04-01 00:00:00

  • Role of the nifB1 and nifB2 Promoters in Cell-Type-Specific Expression of Two Mo Nitrogenases in the Cyanobacterium Anabaena variabilis ATCC 29413.

    abstract::Anabaena variabilis ATCC 29413 has one Mo nitrogenase that is made under oxic growth conditions in specialized cells called heterocysts and a second Mo nitrogenase that is made only under anoxic conditions in vegetative cells. The two large nif gene clusters responsible for these two nitrogenases are under the control...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00674-16

    authors: Vernon SA,Pratte BS,Thiel T

    更新日期:2017-01-30 00:00:00

  • Regulation of chromosome replication in Bacillus subtilis: effects of amino acid starvation in strain W23.

    abstract::Amino acid starvation allows limited synthesis of deoxyribonucleic acid (DNA) in Bacillus subtilis strain W23. DNA synthesis increased by about 30% after leucine starvation and by about 60% after histidine starvation. Genetic analysis on the DNA synthesized after amino acid starvation showed that all genetic markers e...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.105.2.595-603.1971

    authors: Copeland JC

    更新日期:1971-02-01 00:00:00

  • Molecular mechanism of tryptophan-dependent transcriptional regulation in Chlamydia trachomatis.

    abstract::Tryptophan is an essential amino acid that is required for normal development in Chlamydia species, and tryptophan metabolism has been implicated in chlamydial persistence and tissue tropism. The ability to synthesize tryptophan is not universal among the Chlamydiaceae, but species that have a predicted tryptophan bio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01660-05

    authors: Akers JC,Tan M

    更新日期:2006-06-01 00:00:00

  • Lignin peroxidase gene family of Phanerochaete chrysosporium: complex regulation by carbon and nitrogen limitation and identification of a second dimorphic chromosome.

    abstract::Lignin peroxidases (LiP) of Phanerochaete chrysosporium are encoded by a family of six closely related genes. Five LiP genes have been localized to the same dimorphic chromosome. In this investigation, relative transcript levels of the LiP genes were determined. Transcripts of the LiPA, LiPB, and O282 genes were at si...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.15.5036-5042.1992

    authors: Stewart P,Kersten P,Vanden Wymelenberg A,Gaskell J,Cullen D

    更新日期:1992-08-01 00:00:00

  • A novel lipolytic enzyme, YcsK (LipC), located in the spore coat of Bacillus subtilis, is involved in spore germination.

    abstract::The predicted amino acid sequence of Bacillus subtilis ycsK exhibits similarity to the GDSL family of lipolytic enzymes. Northern blot analysis showed that ycsK mRNA was first detected from 4 h after the onset of sporulation and that transcription of ycsK was dependent on SigK and GerE. The fluorescence of the YcsK-gr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01527-06

    authors: Masayama A,Kuwana R,Takamatsu H,Hemmi H,Yoshimura T,Watabe K,Moriyama R

    更新日期:2007-03-01 00:00:00

  • Tryptophanase-tryptophan synthetase systems in Escherichia coli. III. Requirements for enzyne synthesis.

    abstract::Freundlich, Martin (University of Minnesota, Minneapolis) and Herman C. Lichstein. Tryptophanase-tryptophan synthetase systems in Escherichia coli. III. Requirements for enzyme synthesis. J. Bacteriol. 84:996-1006. 1962.-The requirements for the formation of tryptophanase and tryptophan synthetase in Escherichia coli ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.84.5.996-1006.1962

    authors: FREUNDLICH M,LICHSTEIN HC

    更新日期:1962-11-01 00:00:00

  • Temperature-sensitive mutants of Escherichia coli K-12 with low activity of the diaminopimelic acid adding enzyme.

    abstract::Five temperature-sensitive lysis mutants were found to possess very low diaminopimelic acid (Dpm) adding enzyme activity in vitro. Murein synthesis at 42 C was impaired, and uridine-5'-diphosphate-N-acetyl-muramyl-l-Ala- d-Glu (UDP-MurNAc-dipeptide) was accumulated. In the presence of NaCl, the mutants could grow at 4...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.110.1.41-46.1972

    authors: Lugtenberg EJ,v Schijndel-van Dam A

    更新日期:1972-04-01 00:00:00

  • Ferric reductase activity in Azotobacter vinelandii and its inhibition by Zn2+.

    abstract::Ferric reductase activity was examined in Azotobacter vinelandii and was found to be located in the cytoplasm. The specific activities of soluble cell extracts were not affected by the iron concentration of the growth medium; however, activity was inhibited by the presence of Zn2+ during cell growth and also by the ad...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.7.4031-4037.1989

    authors: Huyer M,Page WJ

    更新日期:1989-07-01 00:00:00