Abstract:
:The predominant mode of growth of bacteria in the environment is within sessile, matrix-enclosed communities known as biofilms. Biofilms often complicate chronic and difficult-to-treat infections by protecting bacteria from the immune system, decreasing antibiotic efficacy, and dispersing planktonic cells to distant body sites. While the biology of bacterial biofilms has become a major focus of microbial research, the regulatory mechanisms of biofilm development remain poorly defined and those of dispersal are unknown. Here we establish that the RNA binding global regulatory protein CsrA (carbon storage regulator) of Escherichia coli K-12 serves as both a repressor of biofilm formation and an activator of biofilm dispersal under a variety of culture conditions. Ectopic expression of the E. coli K-12 csrA gene repressed biofilm formation by related bacterial pathogens. A csrA knockout mutation enhanced biofilm formation in E. coli strains that were defective for extracellular, surface, or regulatory factors previously implicated in biofilm formation. In contrast, this csrA mutation did not affect biofilm formation by a glgA (glycogen synthase) knockout mutant. Complementation studies with glg genes provided further genetic evidence that the effects of CsrA on biofilm formation are mediated largely through the regulation of intracellular glycogen biosynthesis and catabolism. Finally, the expression of a chromosomally encoded csrA'-'lacZ translational fusion was dynamically regulated during biofilm formation in a pattern consistent with its role as a repressor. We propose that global regulation of central carbon flux by CsrA is an extremely important feature of E. coli biofilm development.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Jackson DW,Suzuki K,Oakford L,Simecka JW,Hart ME,Romeo Tdoi
10.1128/jb.184.1.290-301.2002keywords:
subject
Has Abstractpub_date
2002-01-01 00:00:00pages
290-301issue
1eissn
0021-9193issn
1098-5530journal_volume
184pub_type
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journal_title:Journal of bacteriology
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doi:10.1128/jb.166.1.334-337.1986
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doi:10.1128/JB.105.1.445-448.1971
更新日期:1971-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.172.5.2259-2266.1990
更新日期:1990-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1980-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2008-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.174.3.867-872.1992
更新日期:1992-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2000-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2003-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.173.1.283-290.1991
更新日期:1991-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2003-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.141.1.184-189.1980
更新日期:1980-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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abstract::The recent emergence of multidrug resistance (MDR) in Acinetobacter baumannii has raised concern in health care settings worldwide. In order to understand the repertoire of resistance determinants and their organization and origins, we compared the genome sequences of three MDR and three drug-susceptible A. baumannii ...
journal_title:Journal of bacteriology
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更新日期:2008-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.129.2.901-907.1977
更新日期:1977-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.135.3.1008-1014.1978
更新日期:1978-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.160.1.279-287.1984
更新日期:1984-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.22.7245-7252.1992
更新日期:1992-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.160.2.818-821.1984
更新日期:1984-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2005-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2013-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1974-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.7.1981-1987.1993
更新日期:1993-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.9.4376-4378.1987
更新日期:1987-09-01 00:00:00
abstract::Protein 1, a major protein of the outer membrane of Escherichia coli, has been shown to be the pore allowing the passage of small hydrophilic solutes across the outer membrane. In E. coli K-12 protein 1 consists of two subspecies, 1a and 1b, whereas in E. coli B it consists of a single species which has an electrophor...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.133.3.1181-1189.1978
更新日期:1978-03-01 00:00:00