Mechanism of ToxT-dependent transcriptional activation at the Vibrio cholerae tcpA promoter.

abstract：:Two nrdF genes, nrdF1 and nrdF2, encoding the small subunit (R2) of ribonucleotide reductase (RR) from Mycobacterium tuberculosis have 71% identity at the amino acid level and are both highly homologous with Salmonella typhimurium R2F. The calculated molecular masses of R2-1 and R2-2 are 36,588 (322 amino acids [aa]) ...

journal_title：Journal of bacteriology

authors： Yang F,Curran SC,Li LS,Avarbock D,Graf JD,Chua MM,Lu G,Salem J,Rubin H

更新日期：1997-10-01 00:00:00

abstract：:Broad-host-range plasmid RK2 encodes several different kil genes which are potentially lethal to an Escherichia coli host. The kil genes and the essential RK2 replication gene trfA are regulated by the products of kor genes. We have shown previously that kilA can be controlled by a constitutively expressed korA gene. ...

journal_title：Journal of bacteriology

authors： Young C,Burlage RS,Figurski DH

更新日期：1987-03-01 00:00:00

abstract：:In mycobacterial growth medium 40 to 400 microM citrate was required to solubilize 2 microM 55Fe. This solubilized 55Fe was taken up into both iron-deficient and iron sufficient washed cell suspensions of Mycobacterium smegmatis and Mycobacterium bovis BCG. Although the 55Fe was taken up into the cell, the citrate was...

journal_title：Journal of bacteriology

authors： Messenger AJ,Ratledge C

更新日期：1982-01-01 00:00:00

abstract：:Binding sites for the Escherichia coli protein integration host factor (IHF) include a set of conserved bases that can be summarized by the consensus sequence WATCAANNNNTTR (W is dA or dT, R is dA or dG, and N is any nucleotide). However, additional 5'-proximal bases, whose common feature is a high dA+dT content, are ...

journal_title：Journal of bacteriology

authors： Hales LM,Gumport RI,Gardner JF

更新日期：1994-05-01 00:00:00

abstract：:Autoinducer-2 (AI-2) is required for biofilm formation and virulence of the oral pathogen Aggregatibacter actinomycetemcomitans, and we previously showed that lsrB codes for a receptor for AI-2. The lsrB gene is expressed as part of the lsrACDBFG operon, which is divergently transcribed from an adjacent lsrRK operon. ...

journal_title：Journal of bacteriology

authors： Torres-Escobar A,Juárez-Rodríguez MD,Lamont RJ,Demuth DR

更新日期：2013-01-01 00:00:00

abstract：:The function of AI-2 in many bacteria and the physiological role of LuxS, the enzyme responsible for its production, remain matters of debate. Here, we show that in Staphylococcus aureus the luxS gene forms a monocistronic transcriptional unit under the control of a sigma(70)-dependent promoter. The gene was transcrib...

journal_title：Journal of bacteriology

authors： Doherty N,Holden MT,Qazi SN,Williams P,Winzer K

更新日期：2006-04-01 00:00:00

abstract：:Escherichia coli BS-12 uvrA lon is hypersensitive to ultraviolet light. On minimal agar plates at densities in excess of about 10(7) bacteria per plate, as few as one or two photoreversible pyrimidine dimers in the entire genome are sufficient to cause inhibition of cell division. Most of the resulting filaments are u...

journal_title：Journal of bacteriology

authors： Bridges BA,Mottershead RP,Green MH

更新日期：1977-05-01 00:00:00

abstract：:The cloned gene coding for Bacillus licheniformis penicillinase (penP) was introduced into Escherichia coli in a heat-inducible lambda Qam vector. After induction, significant amounts of penicillinase were synthesized in the new host. The cellular location of the penicillinase was found to be almost exclusively the ou...

journal_title：Journal of bacteriology

authors： Sarvas MO,Palva IA

更新日期：1983-08-01 00:00:00

abstract：:Cotransformation frequencies of 16, 39, 51, and 60% were observed when donor alleles were separated by distances of 9.2, 7.4, 6.3, and 5.1 kb, respectively, in donor Acinetobacter calcoaceticus DNA. A different and unexpected pattern was observed when the distance between recipient alleles was reduced from 9.2 to 5.1 ...

journal_title：Journal of bacteriology

authors： Kloos DU,DiMarco AA,Elsemore DA,Timmis KN,Ornston LN

更新日期：1995-10-01 00:00:00

abstract：:The bacterium Rhodobacter capsulatus is capable of an unusual form of genetic exchange, mediated by a transducing bacteriophage-like particle called the gene transfer agent (GTA). GTA production by R. capsulatus is controlled at the level of transcription by a cellular two-component signal transduction system that inc...

journal_title：Journal of bacteriology

authors： Lang AS,Beatty JT

更新日期：2002-02-01 00:00:00

abstract：:One of the pleiotropic phenotypes of mutations affecting DNA gyrase activity in Salmonella typhimurium is the constitutive deattenuation of the histidine operon. In the present work, we isolated and characterized a suppressor mutation which restores his attenuation in the presence of a defective gyrase. Such a suppres...

journal_title：Journal of bacteriology

authors： Blanc-Potard AB,Bossi L

更新日期：1994-04-01 00:00:00

abstract：:The gene for an extremely thermostable oligo-1,6-glucosidase (dextrin-6-alpha-D-glucanohydrolase; EC 3.2.1.10) of obligately thermophilic Bacillus thermoglucosidasius KP1006 was cloned within a 4.2-kilobase HindIII-PvuII fragment of DNA by using the plasmid pUC19 as a vector and Escherichia coli C600 as a host. The ge...

journal_title：Journal of bacteriology

authors： Watanabe K,Iha H,Ohashi A,Suzuki Y

更新日期：1989-02-01 00:00:00

abstract：:The conversion of Pseudomonas aeruginosa PAO to the mucoid phenotype has been reported for a chronic pulmonary infection model in rats (D. E. Woods, P. A. Sokol, L. E. Bryan, D. G. Storey, S. J. Mattingly, H. J. Vogel, and H. Ceri, J. Infect. Dis. 163:143-149, 1991). This conversion was associated with a genetic rearr...

journal_title：Journal of bacteriology

authors： Sokol PA,Luan MZ,Storey DG,Thirukkumaran P

更新日期：1994-02-01 00:00:00

abstract：:Starvation of Escherichia coli for potassium, phosphate, or magnesium ions leads to a reversible increase in the rate of protein degradation and an inhibition of ribonucleic acid (RNA) synthesis. In cells deprived of potassium, the breakdown of the more stable cell proteins increased two- to threefold, whereas the hyd...

journal_title：Journal of bacteriology

authors： St John AC,Goldberg AL

更新日期：1980-09-01 00:00:00

abstract：:Molybdenum (Mo) is an important trace element that is toxic at high concentrations. To resolve the mechanisms underlying Mo toxicity, Rhodobacter capsulatus mutants tolerant to high Mo concentrations were isolated by random transposon Tn5 mutagenesis. The insertion sites of six independent isolates mapped within the s...

journal_title：Journal of bacteriology

authors： Gisin J,Müller A,Pfänder Y,Leimkühler S,Narberhaus F,Masepohl B

更新日期：2010-11-01 00:00:00

abstract：:Colicin E2 was partially purified from Escherichia coli W3110. This preparation was remarkably stabilized by bovine serum albumin in a solution at neutral pH, as shown by dilution experiments and tests on heat stability of colicin. One killing unit of colicin E2 was estimated to correspond to one molecule of colicin E...

journal_title：Journal of bacteriology

authors： Mitsui E,Mizuno D

更新日期：1969-11-01 00:00:00

abstract：:We have constructed Escherichia coli strains containing mutations at two different loci, both originally selected for failure to support lambda site-specific recombination: himA and gyrB-him(Ts). Although the gyrB-him(Ts) mutations by themselves reduce supercoiling at high temperature, the double mutants show a far gr...

journal_title：Journal of bacteriology

authors： Friedman DI,Olson EJ,Carver D,Gellert M

更新日期：1984-02-01 00:00:00

abstract：:Penicillin-binding proteins of 180, 89, 80, 68, 61, 41, and 38 kilodaltons were identified in Treponema pallidum (Nichols) by their covalent binding of [35S]benzylpenicillin. Penicillin-binding proteins are localized in the plasma membranes of many bacterial species and may serve as useful markers for determining plas...

journal_title：Journal of bacteriology

authors： Cunningham TM,Miller JN,Lovett MA

更新日期：1987-11-01 00:00:00

abstract：:The fatty acid composition of the membranes of three different penicillin-produced L-forms of Streptococcus faecalis was determined: (i) a stable (nonreverting) L-form (T(53)) cultured in brain heart infusion (BHI) with 0.5 M sucrose; (ii) a stable L-form (T(531)) cultured in BHI without sucrose; and (iii) an unstable...

journal_title：Journal of bacteriology

authors： Montgomerie JZ,Kalmanson GM,Guze LB

更新日期：1973-07-01 00:00:00

abstract：:The EcfG-type sigma factor RpoE2 is the regulator of the general stress response in Sinorhizobium meliloti. RpoE2 activity is negatively regulated by two NepR-type anti-sigma factors (RsiA1/A2), themselves under the control of two anti-anti-sigma factors (RsiB1/B2) belonging to the PhyR family of response regulators. ...

journal_title：Journal of bacteriology

authors： Sauviac L,Bruand C

更新日期：2014-07-01 00:00:00

abstract：UNLABELLED:It is well established that the RpoN-RpoS sigma factor (σ(54)-σ(S)) cascade plays an essential role in differential gene expression during the enzootic cycle of Borrelia burgdorferi, the causative agent of Lyme disease. The RpoN-RpoS pathway is activated by the response regulator/σ(54)-dependent activator (a...

journal_title：Journal of bacteriology

authors： Yin Y,Yang Y,Xiang X,Wang Q,Yang ZN,Blevins J,Lou Y,Yang XF

更新日期：2016-04-28 00:00:00

abstract：:Twelve different dominant negative mutants of the Escherichia coli nucleoid-associated protein, H-NS, have been selected and characterized in vivo. The mutants are all severely defective in promoter repression activity in a strain lacking H-NS, and they all disrupt the repression normally exerted by H-NS at two of its...

journal_title：Journal of bacteriology

authors： Williams RM,Rimsky S,Buc H

更新日期：1996-08-01 00:00:00

abstract：:At specific times in the cell cycle, the bacterium Caulobacter crescentus assembles two major polar organelles, the flagellum and the stalk. Previous studies have shown that flbT mutants overproduce flagellins and are unable to form chemotaxis swarm rings. In this paper, we report alterations in both the stalk and the...

journal_title：Journal of bacteriology

authors： Driks A,Schoenlein PV,DeRosier DJ,Shapiro L,Ely B

更新日期：1990-04-01 00:00:00

abstract：:Iron-sulfur flavoproteins (ISF) constitute a widespread family of redox-active proteins in anaerobic prokaryotes. Based on sequence homologies, their overall structure is expected to be similar to that of flavodoxins, but in addition to a flavin mononucleotide cofactor they also contain a cubane-type [4Fe:4S] cluster....

journal_title：Journal of bacteriology

authors： Andrade SL,Cruz F,Drennan CL,Ramakrishnan V,Rees DC,Ferry JG,Einsle O

更新日期：2005-06-01 00:00:00

abstract：:The distribution of the extracellular enzyme, thiaminase I, was determined for logarithmically growing cultures of Bacillus thiaminolyticus. About 60% of the enzyme is associated with the cells throughout the growth cycle. The remainder of the enzyme is in the culture medium. The release of the cell-bound thiaminase I...

journal_title：Journal of bacteriology

authors： Agee CC,Wilkins JH,Airth RL

更新日期：1973-09-01 00:00:00

abstract：:The Escherichia coli gene coding for dihydropteroate synthase (DHPS) has been cloned and sequenced. The protein has 282 amino acids and a compositional molecular mass of 30,314 daltons. Increased expression of the enzyme was realized by using a T7 expression system. The enzyme was purified and crystallized. A temperat...

journal_title：Journal of bacteriology

authors： Dallas WS,Gowen JE,Ray PH,Cox MJ,Dev IK

更新日期：1992-09-01 00:00:00

abstract：:Studies of gene expression using fusions to lacZ demonstrated that the Bacillus subtilis dacB gene, encoding penicillin-binding protein 5*, is in an operon with two downstream genes, spmA and spmB. Mutations affecting any one of these three genes resulted in the production of spores with reduced heat resistance. The c...

journal_title：Journal of bacteriology

authors： Popham DL,Illades-Aguiar B,Setlow P

更新日期：1995-08-01 00:00:00

abstract：:Features of the amino acid sequence of the TnaC nascent peptide are recognized by the translating ribosome. Recognition leads to tryptophan binding within the translating ribosome, inhibiting the termination of tnaC translation and preventing Rho-dependent transcription termination in the tna operon leader region. It ...

journal_title：Journal of bacteriology

authors： Cruz-Vera LR,New A,Squires C,Yanofsky C

更新日期：2007-04-01 00:00:00

abstract：:The Arc (anoxic redox control) two-component signal transduction system of Escherichia coli, which comprises the tripartite ArcB sensor kinase and the ArcA response regulator, modulates the expression of numerous operons in response to redox conditions of growth. We demonstrate that the arcA and arcB genes of Haemophi...

journal_title：Journal of bacteriology

authors： Georgellis D,Kwon O,Lin EC,Wong SM,Akerley BJ

更新日期：2001-12-01 00:00:00

abstract：:The reactive alpha-oxoaldehydes such as glyoxal (GO) and methylglyoxal (MG) are generated in vivo from sugars through oxidative stress. GO and MG are believed to be removed from cells by glutathione-dependent glyoxalases and other aldehyde reductases. We isolated a number of GO-resistant (GO(r)) mutants from Escherich...

journal_title：Journal of bacteriology

authors： Lee C,Kim I,Lee J,Lee KL,Min B,Park C

更新日期：2010-08-01 00:00:00