Cell-bound thiaminase I of Bacillus thiaminolyticus.

Abstract:

:The distribution of the extracellular enzyme, thiaminase I, was determined for logarithmically growing cultures of Bacillus thiaminolyticus. About 60% of the enzyme is associated with the cells throughout the growth cycle. The remainder of the enzyme is in the culture medium. The release of the cell-bound thiaminase I is examined under a variety of conditions. The rate and extent of release is dependent on the pH and the nature of the incubation solution. The release process appears to be relatively independent of de novo protein synthesis, energy derived from oxidative phosphorylation, or divalent metal ions. The absence of carbon or nitrogen sources has little effect on the release of the enzyme. Cell-bound thiaminase I probably is the immediate precursor for extracellular thiaminase I found in the culture medium. Washed cells continue to release thiaminase I at the expense of cell-bound enzyme. In addition, purified cell-bound thiaminase I is indistinguishable from purified extracellular thiaminase I by a number of physical and kinetic criteria.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Agee CC,Wilkins JH,Airth RL

doi

10.1128/JB.115.3.949-956.1973

subject

Has Abstract

pub_date

1973-09-01 00:00:00

pages

949-56

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

115

pub_type

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