Abstract:
:Under static growth conditions, hyperpiliated, nontwitching pilT and pilU mutants of Pseudomonas aeruginosa formed dense biofilms, showing that adhesion, not twitching motility, is necessary for biofilm initiation. Under flow conditions, the pilT mutant formed mushroom-like structures larger than those of the wild type but the pilU mutant was defective in biofilm formation. Therefore, twitching motility affects the development of biofilm structure, possibly through modulation of detachment.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Chiang P,Burrows LLdoi
10.1128/jb.185.7.2374-2378.2003keywords:
subject
Has Abstractpub_date
2003-04-01 00:00:00pages
2374-8issue
7eissn
0021-9193issn
1098-5530journal_volume
185pub_type
杂志文章abstract::Scanning electron microscopy was used to examine the major stages of the life cycle of two wild-type strains of Neurospora crassa Shear and Dodge (St. Lawrence 3.1a and 74A): mycelia, protoperithecium formation, perithecia, ascospores, ascospore germination and outgrowth, macro and microconidia, and germination and ou...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.113.2.1015-1025.1973
更新日期:1973-02-01 00:00:00
abstract::Pseudomonas fluorescens strain KU-7 is a prototype microorganism that metabolizes 2-nitrobenzoate (2-NBA) via the formation of 3-hydroxyanthranilate (3-HAA), a known antioxidant and reductant. The initial two steps leading to the sequential formation of 2-hydroxy/aminobenzoate and 3-HAA are catalyzed by a NADPH-depend...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01098-06
更新日期:2007-05-01 00:00:00
abstract::Ramakrishnan, T. (Yale University, New Haven, Conn.), and Edward A. Adelberg. Regulatory mechanisms in the biosynthesis of isoleucine and valine. II. Identification of two operator genes. J. Bacteriol. 89:654-660. 1965.-A tightly clustered set of five structural genes governs the synthesis of the five enzymes of isole...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.3.654-660.1965
更新日期:1965-03-01 00:00:00
abstract::Caulobacter crescentus trp mutants were identified from a collection of auxotrophs. Precursor feeding experiments, accumulation studies, and complementation experiments resulted in the identification of six genes corresponding to trpA, trpB, trpC, trpD, trpE, and trpF. Genetic mapping experiments demonstrated that the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.160.1.279-287.1984
更新日期:1984-10-01 00:00:00
abstract::The ribosomal proteins from nine species of prokaryotes have been compared by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. The stained gels were scanned spectrophotometrically, the weight and number average molecular weights were calculated, and the detailed distribution of the protein...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.111.2.474-480.1972
更新日期:1972-08-01 00:00:00
abstract::Saccharomyces cerevisiae cells, grown on a fermentable carbon source, display two growth phases before they enter the stationary phase: a rapid phase (log phase) followed by a slow phase. It was previously shown that a subunit of the yeast RNA polymerase II, RPB4, positively affects the activity of the enzyme in post-...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.19.6358-6363.1993
更新日期:1993-10-01 00:00:00
abstract::Anthranilate (2-aminobenzoate) is an important intermediate in tryptophan metabolism. In order to investigate the degradation of tryptophan through anthranilate by Burkholderia cepacia, several plasposon mutations were constructed of strain DBO1 and one mutant with the plasposon insertion in the anthranilate dioxygena...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.19.5871-5881.2003
更新日期:2003-10-01 00:00:00
abstract::Mutations in the spoIIIA and spoIIIJ loci disrupt the compartmentalization of gene expression during sporulation of Bacillus subtilis. The breakdown in compartmentalization is not the cause of their being blocked in spore formation. Rather, it appears to be a consequence of the engulfed prespore's being unstable. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.7.2221-2223.2003
更新日期:2004-04-01 00:00:00
abstract::We have cloned and sequenced a 505-base-pair (bp) segment of DNA situated upstream of mvaA, the structural gene for (S)-3-hydroxy-3-methylglutaryl coenzyme A reductase (EC 1.1.1.88) of Pseudomonas mevalonii. The DNA segment that we characterized includes the promoter region for the mva operon. Nuclease S1 mapping and ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.10.5567-5571.1989
更新日期:1989-10-01 00:00:00
abstract::We isolated 28 mutants of Bacillus subtilis deficient in the development of competence by using the transposon Tn917lacZ as a mutagen. The mutant strains were poorly transformable with plasmid and chromosomal DNAs but were normally transducible and exhibited wild-type resistance to DNA-damaging agents. The mutations w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.7.3104-3109.1987
更新日期:1987-07-01 00:00:00
abstract::Aerobacter cloacae UW-C83 and Escherichia coli K-12 were grown under various anaerobic environments. Y(atp) values were calculated by determination of cell weights and analyses for fermentation products. These Y(atp) values are compared with others reported in the literature. Limitation of growth by factors other than...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.4.991-995.1967
更新日期:1967-10-01 00:00:00
abstract::The mechanism of action of diphtheria toxin in an Escherichia coli cell-free protein-synthesizing system was examined. When the washed ribosomes were incubated with toxin before addition of messenger ribonucleic acid (RNA), peptide syntheses of (14)C-phenylalanine directed by polyuridylic acid or phage R17 RNA were st...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.104.1.152-157.1970
更新日期:1970-10-01 00:00:00
abstract::The WO-1 strain of Candida albicans is capable of alternating between two highly distinct yeast cell types termed white and opaque (E. H. A. Rikkerrink, B. B. Magee, and P. T. Magee, J. Bacteriol. 170:895-899, 1988; B. Slutsky, M. Staebell, J. Anderson, L. Risen, M. Pfaller, and D. R. Soll, J. Bacteriol. 169:189-197, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.23.7436-7442.1991
更新日期:1991-12-01 00:00:00
abstract::Massetolide A is a cyclic lipopeptide (CLP) antibiotic produced by various Pseudomonas strains from diverse environments. Cloning, sequencing, site-directed mutagenesis, and complementation showed that massetolide A biosynthesis in P. fluorescens SS101 is governed by three nonribosomal peptide synthetase (NRPS) genes,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01563-07
更新日期:2008-04-01 00:00:00
abstract::An increase in the apparent molecular weight of the principal outer membrane protein (POMP) of Neisseria gonorrhoeae is associated with introduction of the penB2 genetic marker, which results in low-level, relatively nonspecific antibiotic resistance. Limited proteolysis of the two forms of POMP showed that they had f...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.143.2.847-851.1980
更新日期:1980-08-01 00:00:00
abstract::The gene encoding Neisseria gonorrhoeae periplasmic binding protein FbpA contains two regions whose sequences exhibit homology with the Escherichia coli ferric uptake regulator protein (Fur) consensus binding sequence. In this study, DNase I footprinting experiments were employed to characterize the operator sequences...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.16.5020-5023.1996
更新日期:1996-08-01 00:00:00
abstract::The endophytic diazotroph Azoarcus sp. strain BH72 is capable of infecting rice roots and of expressing the nitrogenase (nif) genes there. In order to study the genetic background for nitrogen fixation in strain BH72, the structural genes of nitrogenase (nifHDK) were cloned and sequenced. The sequence analysis reveale...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.12.3752-3760.2001
更新日期:2001-06-01 00:00:00
abstract::"Roseburia inulinivorans" is an anaerobic polysaccharide-utilizing firmicute bacterium from the human colon that was identified as a producer of butyric acid during growth on glucose, starch, or inulin. R. inulinivorans A2-194 is also able to grow on the host-derived sugar fucose, following a lag period, producing pro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00137-06
更新日期:2006-06-01 00:00:00
abstract::Bacterial Gre factors associate with RNA polymerase (RNAP) and stimulate intrinsic cleavage of the nascent transcript at the active site of RNAP. Biochemical and genetic studies to date have shown that Escherichia coli Gre factors prevent transcriptional arrest during elongation and enhance transcription fidelity. Fur...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00086-11
更新日期:2011-06-01 00:00:00
abstract::The Yersinia adhesin A (YadA) is a trimeric autotransporter adhesin of enteric yersiniae. It consists of three major domains: a head mediating adherence to host cells, a stalk involved in serum resistance, and an anchor that forms a membrane pore and is responsible for the autotransport function. The anchor contains a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00985-07
更新日期:2007-12-01 00:00:00
abstract::Cells of Saccharomyces cerevisiae permeabilized by treatment with ether take up and incorporate exogenous deoxynucleoside triphosphate into deoxyribonucleic acid (DNA). With rho(+) strains, more than 95% of the product was mitochondrial DNA (mtDNA). This report characterizes ether-permeabilized yeast cells and describ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.140.2.333-341.1979
更新日期:1979-11-01 00:00:00
abstract::A novel sucrose hydrolase (SUH) from Xanthomonas axonopodis pv. glycines, a causative agent of bacterial pustule disease on soybeans, was studied at the functional and molecular levels. SUH was shown to act rather specifically on sucrose (K(m) = 2.5 mM) but not on sucrose-6-phosphate. Protein analysis of purified SUH ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.2.411-418.2004
更新日期:2004-01-01 00:00:00
abstract::Ultrastructural and biochemical studies of a recombinant Treponema pallidum surface antigen designated 4D have been conducted due to its likely biological significance. Electron microscopy demonstrated that the 190-kilodalton (kDa) 4D molecule is an ordered ring structure of 10-nm diameter. The 90-kDa proteinase K-tre...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.165.3.732-739.1986
更新日期:1986-03-01 00:00:00
abstract::Pizer, Lewis I. (University of Pennsylvania, Philadelphia). Glycine synthesis and metabolism in Escherichia coli. J. Bacteriol. 89:1145-1150. 1965.-A correlation was demonstrated between a nutritional requirement that can only be satisfied by glycine and the absence of the enzymatic capacity to interconvert l-serine a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.4.1145-1150.1965
更新日期:1965-04-01 00:00:00
abstract::An attempt was made to explain the puzzling observation that in bacteria 2,6-diaminopurine can replace guanine for guanineless mutants and for xanthineless mutants (both of which can make adenosine monophosphate de novo) but not for nonexacting purine auxotrophs (which cannot make adenosine monophosphate de novo). The...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.2.565-571.1968
更新日期:1968-02-01 00:00:00
abstract::The major coat protein of the bacteriophage f1 is synthesized during infection of Escherichia coli and becomes tightly associated with the host membrane. This synthesis was studied in conjunction with the strain BB26-36, a mutant defective in phospholipid synthesis, to investigate basic questions concerning membrane p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.129.3.1245-1249.1977
更新日期:1977-03-01 00:00:00
abstract::Desulfurococcus kamchatkensis is an anaerobic organotrophic hyperthermophilic crenarchaeon isolated from a terrestrial hot spring. Its genome consists of a single circular chromosome of 1,365,223 bp with no extrachromosomal elements. A total of 1,474 protein-encoding genes were annotated, among which 205 are exclusive...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01525-08
更新日期:2009-04-01 00:00:00
abstract::Fukuyama T. T. (University of Pennsylvania, Philadelphia) and D. J. O'Kane. Galactose metabolism. I. Pathway of carbon in fermentation by Streptococcus faecalis. J. Bacteriol. 84:793-796. 1962.-The pathway by which galactose-1-C(14) is fermented in Streptococcus faecalis was investigated using dried-cell preparations....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.84.4.793-796.1962
更新日期:1962-10-01 00:00:00
abstract::The human pathogen Eikenella corrodens synthesizes type IV pili and exhibits a phase variation involving the irreversible transition from piliated to nonpiliated variants. On solid medium, piliated variants form small (S-phase), corroding colonies whereas nonpiliated variants form large (L-phase), noncorroding colonie...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.14.4154-4160.1999
更新日期:1999-07-01 00:00:00
abstract::At a pH of journal_title:Journal of bacteriology pub_type: 杂志文章 doi:10.1128/jb.184.19.5339-5347.2002 更新日期:2002-10-01 00:00:00