Isolation and characterization of Tn917lac-generated competence mutants of Bacillus subtilis.

abstract：:Site-directed mutagenesis has been employed to address the functional significance of the highly conserved aspartic and glutamic acid residues present in the Walker B (also called motif II) sequence in Escherichia coli DNA helicase II. Two mutant proteins, UvrDE221Q and UvrDD220NE221Q, were expressed and purified to a...

journal_title：Journal of bacteriology

authors： Brosh RM Jr,Matson SW

更新日期：1995-10-01 00:00:00

abstract：:In gram-negative bacteria, a pathway for aerobic degradation of phenylacetic acid (PAA) that proceeds via phenylacetyl-coenzyme A (CoA) and hydrolytic ring fission plays a central role in the degradation of a range of aromatic compounds. In contrast, the PAA pathway and its role are not well characterized in gram-posi...

journal_title：Journal of bacteriology

authors： Navarro-Llorens JM,Patrauchan MA,Stewart GR,Davies JE,Eltis LD,Mohn WW

更新日期：2005-07-01 00:00:00

abstract：:The genetic organization of the polypeptides required for the biosynthesis of the F41 adhesin of enterotoxigenic Escherichia coli strains was investigated. Maxicell analysis demonstrated that a recombinant plasmid which mediated mannose-resistant hemagglutination and F41 antigen production encoded four polypeptides of...

journal_title：Journal of bacteriology

authors： Anderson DG,Moseley SL

更新日期：1988-10-01 00:00:00

abstract：:N15 is the only bacteriophage of Escherichia coli known to lysogenize as a linear plasmid. Clear-plaque mutations lie in at least two regions of the 46-kb genome. We have cloned, sequenced, and characterized the primary immunity region, immB. This region contains a gene, cB, whose product shows homology to lambdoid ph...

journal_title：Journal of bacteriology

authors： Lobocka MB,Svarchevsky AN,Rybchin VN,Yarmolinsky MB

更新日期：1996-05-01 00:00:00

abstract：:The EutD protein of Salmonella enterica is homologous to the catalytic domain of the phosphotransacetylase (Pta) enzyme. The Pta-like activity level of the EutD enzyme compared favorably to that of other Pta enzymes. High-pressure liquid chromatography and mass spectrometry verified that acetyl-coenzyme A was the prod...

journal_title：Journal of bacteriology

authors： Brinsmade SR,Escalante-Semerena JC

更新日期：2004-03-01 00:00:00

abstract：:The photosynthetic bacterium Rhodobacter capsulatus contains two [NiFe]hydrogenases: an energy-generating hydrogenase, HupSL, and a regulatory hydrogenase, HupUV. The synthesis of HupSL is specifically activated by H(2) through a signal transduction cascade comprising three proteins: the H(2)-sensing HupUV protein, th...

journal_title：Journal of bacteriology

authors： Elsen S,Duché O,Colbeau A

更新日期：2003-12-01 00:00:00

abstract：:Proline dehydrogenase (PutA) is a bifunctional enzyme that catalyzes the oxidation of proline to glutamate. In Sinorhizobium meliloti, as in other microorganisms, the putA gene is transcriptionally activated in response to proline. In Rhodobacter capsulatus, Agrobacterium, and most probably in Bradyrhizobium, this act...

journal_title：Journal of bacteriology

authors： Soto MJ,Jiménez-Zurdo JI,van Dillewijn P,Toro N

更新日期：2000-04-01 00:00:00

abstract：:Flagellate bacteria such as Escherichia coli and Salmonella enterica serovar Typhimurium typically express 5 to 12 flagellar filaments over their cell surface that rotate in clockwise (CW) and counterclockwise directions. These bacteria modulate their swimming direction towards favorable environments by biasing the di...

journal_title：Journal of bacteriology

authors： Morehouse KA,Goodfellow IG,Sockett RE

更新日期：2005-03-01 00:00:00

abstract：:In Escherichia coli, the following genes are involved in motility and chemotaxis. The H gene is the structural gene for flagellin. Mutation in the mot gene results in paralysis of the flagella, and mutation in the fla genes leads to an absence of flagella. The cheA, cheB, and cheC genes are required for chemotaxis. Th...

journal_title：Journal of bacteriology

authors： Armstrong JB,Adler J

更新日期：1969-01-01 00:00:00

abstract：:A heat-stable, particulate, lipopolysaccharide-protein antigenic complex has been isolated from a virulent, encapsulated strain of Pasteurella multocida by extraction with cold, formalinized saline, and centrifugation at 105,000 x g. The original bacterial culture had been obtained from a bison that died of hemorrhagi...

journal_title：Journal of bacteriology

authors： Rebers PA,Heddleston KL,Rhoades KR

更新日期：1967-01-01 00:00:00

abstract：:A new osmotically inducible gene in Escherichia coli, osmY, was induced 8- to 10-fold by hyperosmotic stress and 2- to 3-fold by growth in complex medium. The osmY gene product is a periplasmic protein which migrates with an apparent molecular mass of 22 kDa on sodium dodecyl sulfate-polyacrylamide gels. A genetic fus...

journal_title：Journal of bacteriology

authors： Yim HH,Villarejo M

更新日期：1992-06-01 00:00:00

abstract：:Several resistant mutants of a transformable group H streptococcus, strain Challis, were isolated from media containing high concentrations of streptomycin. Mutants SR5a and SR5 exhibited high and low transformability, respectively, when exposed to deoxyribonucleic acid (DNA) from a novobiocin-resistant Challis strain...

journal_title：Journal of bacteriology

authors： Perry D

更新日期：1968-01-01 00:00:00

abstract：:The mutualistic symbiont Vibrio fischeri builds a symbiotic biofilm during colonization of squid hosts. Regulation of the exopolysaccharide component, termed Syp, has been examined in strain ES114, where production is controlled by a phosphorelay that includes the inner membrane hybrid histidine kinase RscS. Most stra...

journal_title：Journal of bacteriology

authors： Rotman ER,Bultman KM,Brooks JF 2nd,Gyllborg MC,Burgos HL,Wollenberg MS,Mandel MJ

更新日期：2019-04-09 00:00:00

abstract：:In Escherichia coli, the GlpT transporter, a member of the major facilitator superfamily, moves external glycerol 3-phosphate (G3P) into the cytoplasm in exchange for cytoplasmic phosphate. Study of intact cells showed that both GlpT and HisGlpT, a variant with an N-terminal six-histidine tag, are inhibited (50% inhib...

journal_title：Journal of bacteriology

authors： Fann MC,Busch A,Maloney PC

更新日期：2003-07-01 00:00:00

abstract：:Several lysosomal glycosidase activities were examined in vitro during heat-induced germination of Dictyostelium discoideum spores and were found not to be coordinately controlled. The level of beta-glucosidase activity increased significantly during the emergence stage of germination. Both alpha-glucosidase and N-ace...

journal_title：Journal of bacteriology

authors： Tisa LS,Cotter DA

更新日期：1980-02-01 00:00:00

abstract：:The csgA gene produces an intercellular signal during fruiting body formation of the myxobacterium Myxococcus xanthus. Sporulating pseudorevertants were isolated to allow us to understand the mechanism by which CsgA is perceived by cells and used to regulate developmental gene expression. Two strains, LS559 and LS560,...

journal_title：Journal of bacteriology

authors： Lee K,Shimkets LJ

更新日期：1994-04-01 00:00:00

abstract：:The protein motive force of metabolizing Bacillus subtilis cells was only slightly affected by changes in the external pH between 5 and 8, although the electrical component and the chemical component of the proton motive force contributed differently at different external pH. The electrical component of the proton mot...

journal_title：Journal of bacteriology

authors： Shioi JI,Matsuura S,Imae Y

更新日期：1980-12-01 00:00:00

abstract：:The gene responsible for the uncommon glutamate dehydrogenase (GDH) activity of Lactococcus lactis was identified and characterized. It encodes a GDH of family I that is mainly active in glutamate biosynthesis, is carried by a large plasmid, and is included, with functional cadmium resistance genes, in a remnant Tn3-l...

journal_title：Journal of bacteriology

authors： Tanous C,Chambellon E,Sepulchre AM,Yvon M

更新日期：2005-07-01 00:00:00

abstract：:The bacteriophage Mu genome contains a centrally located strong gyrase site (SGS) that is required for efficient prophage replication. To aid in studying the unusual properties of the SGS, we sought other gyrase sites that might be able to substitute for the SGS in Mu replication. Five candidate sites were obtained by...

journal_title：Journal of bacteriology

authors： Oram M,Pato ML

更新日期：2004-07-01 00:00:00

abstract：:The bacterial plant pathogen Pseudomonas syringae depends on a type III protein secretion system and the effector proteins that it translocates into plant cells to cause disease and to elicit the defense-associated hypersensitive response on resistant plants. The availability of the P. syringae pv. tomato DC3000 genom...

journal_title：Journal of bacteriology

authors： Wehling MD,Guo M,Fu ZQ,Alfano JR

更新日期：2004-06-01 00:00:00

abstract：:Most bacteriophages (phages) release their progeny through the action of holins that form lesions in the cytoplasmic membrane and lysins that degrade the bacterial peptidoglycan. Although the function of each protein is well established in phages infecting Streptococcus pneumoniae, the role--if any--of the powerful ba...

journal_title：Journal of bacteriology

authors： Frias MJ,Melo-Cristino J,Ramirez M

更新日期：2009-09-01 00:00:00

abstract：:The Escherichia coli adhesin involved in diffuse adherence (AIDA-I) is one of the few glycosylated proteins found in Escherichia coli. Glycosylation is mediated by a specific heptosyltransferase encoded by the aah gene, but little is known about the role of this modification and the mechanism involved. In this study, ...

journal_title：Journal of bacteriology

authors： Charbonneau ME,Girard V,Nikolakakis A,Campos M,Berthiaume F,Dumas F,Lépine F,Mourez M

更新日期：2007-12-01 00:00:00

abstract：:Aronson, John N. (Arizona State University, Tempe), and Gerald R. Wermus. Effects of m-tyrosine on growth and sporulation of Bacillus species. J. Bacteriol. 90:38-46. 1965.-The aromatic amino acid analogue, dl-beta-(3-hydroxyphenyl)alanine (m-tyrosine), reduced sporulation of a strain of Bacillus subtilis to less than...

journal_title：Journal of bacteriology

authors： Aronson JN,Wermus GR

更新日期：1965-07-01 00:00:00

abstract：:The mevalonate diphosphate decarboxylase is an enzyme which converts mevalonate diphosphate to isopentenyl diphosphate, the building block of isoprenoids. We used the Saccharomyces cerevisiae temperature-sensitive mutant defective for mevalonate diphosphate decarboxylase previously described (C. Chambon, V. Ladeveve, ...

journal_title：Journal of bacteriology

authors： Bergès T,Guyonnet D,Karst F

更新日期：1997-08-01 00:00:00

abstract：:Swarmer cell differentiation is a complex process involving the activity of many gene products. In this report, we characterized the genetic locus of Tn5 insertion in each of 12 mutants defective in swarmer cell elongation. The mutations fell into four categories affecting either flagellar biosynthesis or energetics, ...

journal_title：Journal of bacteriology

authors： Belas R,Goldman M,Ashliman K

更新日期：1995-02-01 00:00:00

abstract：:Synthetic analogs of enterochelin (enterobactin) were tested for their ability to support the growth of Escherichia coli K-12 under iron-limiting conditions. The cyclic compound MECAM [1,3,5-N.N'; N"-tris-(2,3-dihydroxybenzoyl)-triamino-methylbenzene] and its N-methyl derivative Me3MECAM promoted growth, whereas the 2...

journal_title：Journal of bacteriology

authors： Heidinger S,Braun V,Pecoraro VL,Raymond KN

更新日期：1983-01-01 00:00:00

abstract：:Two mutations concerned with mannan biosynthesis in the yeast Saccharomyces cerevisiae have been mapping. The mnn2 mutation, which affects the addition to the polysaccharide backbone of the first side-chain D-mannose unit in alpha1-leads to2 linkage, was located on chromosome II linked to the centromere and the gall l...

journal_title：Journal of bacteriology

authors： Ballou DL

更新日期：1975-08-01 00:00:00

abstract：:Heterokaryons of Mucor racemosus were produced by fusion of spheroplasts from two auxotrophic strains of the fungus. Germinated sporangiospores were converted to spheroplasts by using commercial chitinase and Myxobacter AL-1 chitosanase. Spheroplasts from the auxotrophic strains were mixed in a buffered Ca(NO3)2 solut...

journal_title：Journal of bacteriology

authors： Genthner FJ,Borgia PT

更新日期：1978-04-01 00:00:00

abstract：:The minimum sequence of the enzymatic (A) subunit of Shiga toxin (STX) required for activity was investigated by introducing N-terminal and C-terminal deletions in the molecule. Enzymatic activity was assessed by using an in vitro translation system. A 253-amino-acid STX A polypeptide, which is recognized as the enzym...

journal_title：Journal of bacteriology

authors： Haddad JE,al-Jaufy AY,Jackson MP

更新日期：1993-08-01 00:00:00

abstract：:Plasma cells obtained from the peripheral blood of a patient with multiple myeloma was incubated in serum and Krebs-Ringer bicarbonate buffer with (14)C-labeled glucose, acetate, and propionate. Glucose utilization by these cells amounted to 0.5 mumole per hr per 10(8) cells and was mainly via the Embden-Meyerhof path...

journal_title：Journal of bacteriology

authors： Stjernholm RL

更新日期：1967-05-01 00:00:00