Abstract:
:Ramakrishnan, T. (Yale University, New Haven, Conn.), and Edward A. Adelberg. Regulatory mechanisms in the biosynthesis of isoleucine and valine. II. Identification of two operator genes. J. Bacteriol. 89:654-660. 1965.-A tightly clustered set of five structural genes governs the synthesis of the five enzymes of isoleucine and valine biosynthesis in Escherichia coli. Three of the genes governing transaminase B, dehydrase, and threonine deaminase, are controlled by a single operator locus, designated oprA. The structural gene governing the condensing enzyme is controlled by a second operator locus, designated oprB. Both oprA and oprB have been shown to regulate structural genes which are cis, but not trans, to their own operator. No mutations have yet been found which affect the level of reductoisomerase, but the existence of a third operator controlling the synthesis of this enzyme can be inferred. Enzyme derepression resulting from mutations in oprA confers resistance to high levels of valine. Derepression of the condensing enzyme resulting from mutations in oprB confers resistance to low levels of valine, and to alpha-aminobutyric acid. The significance of these findings with respect to the valine sensitivity of E. coli strain K-12 is discussed.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
RAMAKRISHNAN T,ADELBERG EAdoi
10.1128/JB.89.3.654-660.1965keywords:
subject
Has Abstractpub_date
1965-03-01 00:00:00pages
654-60eissn
0021-9193issn
1098-5530journal_volume
89pub_type
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.4.1632-1638.1987
更新日期:1987-04-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/JB.181.12.3830-3832.1999
更新日期:1999-06-01 00:00:00
abstract::Containment strategies for outbreaks of invasive Neisseria meningitidis disease are informed by serogroup assays that characterize the polysaccharide capsule. We sought to uncover the genomic basis of conflicting serogroup assay results for an isolate (M16917) from a patient with acute meningococcal disease. To this e...
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更新日期:2012-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.24.7718-7723.1997
更新日期:1997-12-01 00:00:00
abstract::The alpha-aminoadipate pathway for lysine biosynthesis is present only in fungi. The alpha-aminoadipate reductase (AAR) of this pathway catalyzes the conversion of alpha-aminoadipic acid to alpha-aminoadipic-delta-semialdehyde by a complex mechanism involving two gene products, Lys2p and Lys5p. The LYS2 and LYS5 genes...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.24.7120-7125.2001
更新日期:2001-12-01 00:00:00
abstract::Rifkind, David (University of Colorado Medical Center, Denver), and John D. Palmer. Neutralization of endotoxin toxicity in chick embryos by antibiotics. J. Bacteriol. 92:815-819. 1966.-Three cationic cyclic polypeptide antibiotics, polymyxin B sulfate colistin sulfate, and tyrocidine hydrochloride, were shown to neut...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.4.815-819.1966
更新日期:1966-10-01 00:00:00
abstract::The WalRK two-component system plays important roles in maintaining cell wall homeostasis and responding to antibiotic stress in low-GC Gram-positive bacteria. In the major human pathogen, Streptococcus pneumoniae, phosphorylated WalR(Spn) (VicR) response regulator positively controls the transcription of genes encodi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01690-09
更新日期:2010-05-01 00:00:00
abstract::The yeast two-hybrid assay revealed that Bacillus subtilis DnaD, a possible component of the primosome and required for replication initiation, interacted with DnaA and DnaD itself. The mutant DnaD23 was incapable of interacting with DnaA but retained interaction with the wild-type DnaD. These results suggest that int...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.6.2148-2150.2001
更新日期:2001-03-01 00:00:00
abstract::The mutation sup-3 in Bacillus subtilis suppresses ochre (TAA) mutations at each of three codons in the 5' end of the cat-86 coding sequence. The suppressor is shown to insert lysine at ochre codons. The efficiency of suppression by sup-3 is about 15%, as determined by changing a cat-86 Lys codon (codon 12) to an ochr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.10.5322-5324.1989
更新日期:1989-10-01 00:00:00
abstract::The group A streptococcus (GAS) Streptococcus pyogenes is known to cause self-limiting purulent infections in humans. The role of GAS pili in host cell adhesion and biofilm formation is likely fundamental in early colonization. Pilus genes are found in the FCT (fibronectin-binding protein, collagen-binding protein, an...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06283-11
更新日期:2012-02-01 00:00:00
abstract::Fertility factor F confers bacterial conjugation, a process which involves at least 20 tra genes. Resistance plasmids such as R100, R6-5, and R1 have homology with F in the tra region. Conjugal transfer of these plasmids is, however, repressed, while transfer of F is constitutive. Repression of R transfer is due to th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.2.619-623.1987
更新日期:1987-02-01 00:00:00
abstract::Despite a large number of studies on the role of oxygen in cellular processes, there is no consensus as to how oxygen availability to the cell should be defined, let alone how it should be quantified. Here, a quantitative definition for oxygen availability (perceived aerobiosis) is presented; the definition is based o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.5.1402-1406.2002
更新日期:2002-03-01 00:00:00
abstract::A survey of chromosomal variation in the ST239 clonal group of methicillin-resistant Staphylococcus aureus (MRSA) revealed a novel genetic element, ICE6013. The element is 13,354 bp in length, excluding a 6,551-bp Tn552 insertion. ICE6013 is flanked by 3-bp direct repeats and is demarcated by 8-bp imperfect inverted r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00352-09
更新日期:2009-10-01 00:00:00
abstract::Temperature-sensitive nalA mutants of Escherichia coli have been used to investigate the structure and functions of deoxyribonucleic acid (DNA) gyrase. Extracts of one such mutant (nalA43) had thermosensitive DNA gyrase subunit A activity but normal gyrase subunit B activity, proving definitively that nalA is the stru...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.140.2.424-435.1979
更新日期:1979-11-01 00:00:00
abstract::Tracer studies of pyrimidine biosynthesis in Lactobacillus leichmannii (ATCC 7830) indicated that, while aspartate is utilized in the usual manner, the guanido carbon of arginine, rather than carbon dioxide, is utilized as a pyrimidine precursor. The guanido carbon of arginine also contributes, to some extent, to the ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.96.4.1249-1254.1968
更新日期:1968-10-01 00:00:00
abstract::The minimal origin of replication of the broad-host-range plasmid RK2 has two potential recognition sequences for the DnaA protein of Escherichia coli. DNA transfer by transformation into a dnaA-null mutant of E. coli showed that DnaA protein is needed for replication or maintenance of mini-RK2. We isolated and purifi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.10.4703-4709.1987
更新日期:1987-10-01 00:00:00
abstract::Youmans, Anne S. (Northwestern University Medical School, Chicago, Ill.) and Guy P. Youmans. Effect of mycosuppressin on the respiration and growth of Mycobacterium tuberculosis. J. Bacteriol. 84:708-715. 1962.-A substance, called mycosuppressin, was found in the lungs of guinea pigs and rabbits vaccinated with BCG or...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.84.4.708-715.1962
更新日期:1962-10-01 00:00:00
abstract::Membrane vesicles containing internal alcohol dehydrogenase and nicotinamide adenine dinucleotide (NAD) were prepared from Escherichia coli ML308-225. Upon the addition of ethanol, these vesicles respired and transported several amino acids. Transport rates driven by internally generated reduced NAD (NADH) were about ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.120.2.861-865.1974
更新日期:1974-11-01 00:00:00
abstract::The rice pathogen recognition receptor, XA21, confers resistance to Xanthomonas oryzae pv. oryzae strains producing the type one system-secreted molecule, AvrXA21. X. oryzae pv. oryzae requires a regulatory two-component system (TCS) called RaxRH to regulate expression of eight rax (required for AvrXA21 activity) gene...
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pub_type: 杂志文章
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更新日期:2008-03-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2019-05-22 00:00:00
abstract::Genetic transformation of auxotrophs of the extreme thermophile Thermus thermophilus HB27 to prototrophy was obtained at high frequencies of 10(-2) to 10(-1) when proliferating cell populations were exposed to chromosomal DNA from a nutritionally independent wild-type strain. The transformation frequency was proportio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.166.1.338-340.1986
更新日期:1986-04-01 00:00:00
abstract::Pediococcus halophilus X-160 which lacks catabolite control by glucose was isolated from nature (soy moromi mash). Wild-type strains, in xylose-glucose medium, utilized glucose preferentially over xylose and showed diauxic growth. With wild-type strain I-13, xylose isomerase activity was not induced until glucose was ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.4.1793-1800.1989
更新日期:1989-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.1.197-203.1991
更新日期:1991-01-01 00:00:00
abstract::Malmgren, Richard A. (National Cancer Institute; Bethesda, Md.), Alan S. Rabson, Paula G. Carney, and Frances J. Paul. Immunofluorescence of green monkey kidney cells infected with adenovirus 12 and with adenovirus 12 plus simian virus 40. J. Bacteriol. 91:262-265. 1966.-Immunofluorescence studies of the viral antigen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.1.262-265.1966
更新日期:1966-01-01 00:00:00
abstract::Various methods of inducing autolysis of Escherichia coli cells were investigated, some being described here for the first time. For the autolysis of growing cells only induction methods interfering with the biosynthesis of peptidoglycan were taken into consideration, whereas with harvested cells autolysis was induced...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:
更新日期:1982-10-01 00:00:00
abstract::Although amino acid transport has been extensively studied in bacteria during the past decade, little is known concerning the transport of those amino acids that are biosynthetic intermediates or have multiple fates within the cell. We have studied homoserine and threonine as examples of this phenomenon. Homoserine is...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.117.3.1002-1009.1974
更新日期:1974-03-01 00:00:00
abstract::During colonization of the alfalfa rhizosphere, Pseudomonas fluorescens F113 undergoes phenotypic variation, resulting in the appearance of colonies with different morphology. Among phenotypic variants, three isolates, C, F, and S were selected, with the C variant showing colony morphology identical to that of the ino...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.6.1587-1596.2002
更新日期:2002-03-01 00:00:00
abstract::Ruthenium red stained a capsule external to the exine of the Azotobacter cyst. The central body is therefore surrounded by three layers, the intine, the exine, and the capsule, all containing acid mucopolysaccharide. Vesicles that appear to originate from the contracting cell membrane of the central body may account f...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.102.1.234-239.1970
更新日期:1970-04-01 00:00:00
abstract::In Escherichia coli, nine essential cell division proteins are known to localize to the division septum. FtsL is a 13-kDa bitopic membrane protein with a short cytoplasmic N-terminal domain, a membrane-spanning segment, and a periplasmic domain that has a repeated heptad motif characteristic of leucine zippers. Here, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.1.116-129.2000
更新日期:2000-01-01 00:00:00
abstract::Welch, Ann Marie (U. S. Veterans Administration Hospital, Durham, N. C.). Preliminary survey of fungistatic properties of marine algae. J. Bacteriol. 83:97-99. 1962-Homogenized preparations of 35 marine algae were tested for inhibitory activity against 6 pathogenic or opportunistically pathogenic fungi with saturated ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.1.97-99.1962
更新日期:1962-01-01 00:00:00