Abstract:
:Despite a large number of studies on the role of oxygen in cellular processes, there is no consensus as to how oxygen availability to the cell should be defined, let alone how it should be quantified. Here, a quantitative definition for oxygen availability (perceived aerobiosis) is presented; the definition is based on a calibration with reference to the minimal oxygen supply rate needed for fully oxidative catabolism (i.e., complete conversion of the energy source to CO(2) and water for glucose-limited conditions). This quantitative method is used to show how steady-state electron fluxes through the alternative cytochrome oxidases of Escherichia coli are distributed as a function of the extent of aerobiosis of glucose-limited chemostat cultures. At low oxygen availability the electron flux is mainly via the high-affinity cytochrome bd oxidase, and, at higher oxygen availability, a similar phenomenon occurs but now via the low-affinity cytochrome bo oxidase. The main finding is that the catabolic activities of E. coli (and specifically its respiratory activity) are affected by the actual oxygen availability per unit of biomass rather than by the residual dissolved oxygen concentration of the culture.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Alexeeva S,Hellingwerf KJ,Teixeira de Mattos MJdoi
10.1128/jb.184.5.1402-1406.2002keywords:
subject
Has Abstractpub_date
2002-03-01 00:00:00pages
1402-6issue
5eissn
0021-9193issn
1098-5530journal_volume
184pub_type
杂志文章abstract::A mutant requiring isoleucine and valine for growth, because of the absence of acetolactate synthase activity, has been isolated. At least one of three different genes (ilvG, ilvB, ilvI) is required for the expression of acetolactate synthase activity, thus suggesting the presence of three different acetolactate synth...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.120.1.536-538.1974
更新日期:1974-10-01 00:00:00
abstract::A naturally occurring constitutive macrolide-lincosamide-streptogramin B (MLS) resistance plasmid, pNE131, from Staphylococcus epidermidis was chosen to study the molecular basis of constitutive expression. Restriction and functional maps of pNE131 are presented along with the nucleotide sequence of ermM, the gene whi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.166.2.479-483.1986
更新日期:1986-05-01 00:00:00
abstract::Saccharopolyspora spinosa is a Gram-positive bacterium that produces spinosad, a well-known biodegradable insecticide that is used for agricultural pest control and has an excellent environmental and mammalian toxicological profile. Here, we present the first draft genome sequence of the type strain Saccharopolyspora ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00344-11
更新日期:2011-06-01 00:00:00
abstract::The A-subunit polypeptides of Shiga toxin, the Shiga-like toxins (SLTs), and the plant lectin ricin inactivate eucaryotic ribosomes by enzymatically depurinating 28S rRNA. Comparison of the amino acid sequences of the members of the Shiga toxin family and ricin revealed two regions of significant homology that lie wit...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.6.3346-3350.1990
更新日期:1990-06-01 00:00:00
abstract::Martinez, R. J. (University of California, Los Angeles), and E. Z. Gordee. Formation of bacterial flagella. I. Demonstration of a functional flagellin pool in Spirillum serpens and Bacillus subtilis. J. Bacteriol. 91:870-875. 1966-Exponentially growing cultures of Spirillum serpens and Bacillus subtilis regained motil...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.2.870-875.1966
更新日期:1966-02-01 00:00:00
abstract::Expression levels of Bacillus amyloliquefaciens alpha-amylase, Escherichia coli TEM-beta-lactamase, and Semliki Forest virus glycoprotein E1 genes were compared in Bacillus subtilis. All three model genes were expressed by using a secretion vector, constructed by joining the B. amyloliquefaciens alpha-amylase promoter...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.162.1.176-182.1985
更新日期:1985-04-01 00:00:00
abstract:UNLABELLED:Many bacteria produce secreted iron chelators called siderophores, which can be shared among cells with specific siderophore uptake systems regardless of whether the cell produces siderophores. Sharing secreted products allows freeloading, where individuals use resources without bearing the cost of productio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02596-14
更新日期:2015-07-01 00:00:00
abstract::A class II restriction endonuclease which recognizes the same nucleotide sequence as EndoR.HaeIII has been found in four of seven isolates of Neisseria gonorrhoeae tested. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.135.1.270-273.1978
更新日期:1978-07-01 00:00:00
abstract::Acidic hot springs are colonized by a diversity of hyperthermophilic organisms requiring extremes of temperature and pH for growth. To clarify how carbohydrates are consumed in such locations, the structural gene (malA) encoding the major soluble alpha-glucosidase (maltase) and flanking sequences from Sulfolobus solfa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.5.1287-1295.1998
更新日期:1998-03-01 00:00:00
abstract::The heme prosthetic group of heme proteins contains iron, which can be a limiting nutrient. Here, we show that cytochrome c1 protein from Bradyrhizobium japonicum was strongly affected by the iron status, with low expression in cells grown under iron limitation. This control was not affected in mutants encoding the ir...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.15.5084-5089.2005
更新日期:2005-08-01 00:00:00
abstract::The potassium permeability of Rickettsia prowazekii was characterized by chemical measurement of the intracellular sodium and potassium pools and isotopic flux measurements with 86Rb+ as a tracer. R. prowazekii, in contrast to Escherichia coli, did not maintain a high potassium-to-sodium ratio in their cytoplasm excep...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.157.1.197-201.1984
更新日期:1984-01-01 00:00:00
abstract::The generalized transducing phage T4GT7 grew well on recA strains of Escherichia coli and transduced recA into F- and Hfr strains of E. coli at high frequency. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.159.3.1072-1073.1984
更新日期:1984-09-01 00:00:00
abstract::The recA441 mutant of Escherichia coli, which has been thought to have thermoinducible constitutive RecA protease activity, is known to have two mutations within recA. We show here that the mutation that alters codon 38 actually confers temperature-independent constitutive protease activity; the second mutation in rec...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.163.1.407-409.1985
更新日期:1985-07-01 00:00:00
abstract::The Escherichia coli adhesin involved in diffuse adherence (AIDA-I) is a multifunctional autotransporter protein that mediates bacterial aggregation and biofilm formation, as well as adhesion and invasion of cultured epithelial cells. To elucidate the structure-function relationships of AIDA-I, we performed transposon...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01238-07
更新日期:2007-12-01 00:00:00
abstract::Gram-negative Aggregatibacter actinomycetemcomitans can be distinguished (based on the promoter structure of the leukotoxin operon) into JP2 and non-JP2 genotypes, with the former found to be more pathogenic than the latter. Here we report the first complete genome sequence of a serotype b non-JP2 strain of A. actinom...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06770-11
更新日期:2012-04-01 00:00:00
abstract::Rhizobium japonicum 122 DES bacteroids from soybean nodules possess an active H(2)-oxidizing system that recycles all of the H(2) lost through nitrogenase-dependent H(2) evolution. The addition of 72 muM H(2) to suspensions of bacteroids increased O(2) uptake 300% and the rate of C(2)H(2) reduction 300 to 500%. The op...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.137.1.153-160.1979
更新日期:1979-01-01 00:00:00
abstract::Frampton, E. W. (The University of Texas M. D. Anderson Hospital and Tumor Institute, Houston), and B. R. Brinkley. Evidence of lysogeny in derivatives of Escherichia coli. J. Bacteriol. 90:446-452. 1965.-Exposure of cultures of several thymine-requiring derivatives of Escherichia coli strain 15 to radiation [X rays o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.90.2.446-452.1965
更新日期:1965-08-01 00:00:00
abstract::A genomic DNA fragment from Desulfovibrio fructosovorans, which strongly hybridized with the hydAB genes from Desulfovibrio vulgaris Hildenborough, was cloned and sequenced. This fragment was found to contain four genes, named hndA, hndB, hndC, and hndD. Analysis of the sequence homologies indicated that HndA shows 29...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.10.2628-2636.1995
更新日期:1995-05-01 00:00:00
abstract::The vitamin requirements of the group Q streptococci (Streptococcus avium) and the established enterococcal species (S. faecalis and S. faecium) paralleled one another, although S. avium did not characteristically require riboflavine or pyridoxal. S. avium was further differentiated in that it required thymine for gro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.2.297-299.1967
更新日期:1967-08-01 00:00:00
abstract::Clostridium perfringens is an anaerobic Gram-positive pathogen that causes many human and animal diseases, including food poisoning and gas gangrene. C. perfringens lacks flagella but possesses type IV pili (TFP). We have previously shown that C. perfringens can glide across an agar surface in long filaments composed ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01288-12
更新日期:2013-02-01 00:00:00
abstract::Eight metH mutants in Salmonella typhimurium with closely linked sites of mutation which could grow only on methionine were isolated from a metE mutant deficient in N(5)-methyltetrahydropteroyltriglutamate-homocysteine transmethylase; their deficiency in cobalamin-dependent N(5)-methyltetrahydrofolate-homocysteine tra...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.1.377-382.1969
更新日期:1969-10-01 00:00:00
abstract::The ftsB gene of Escherichia coli is believed to be involved in cell division. In this report, we show that plasmids containing the nrdB gene could complement the ftsB mutation, suggesting that ftsB is an allele of nrdB. We compared changes in the cell shape of isogenic nrdA, nrdB, ftsB, and pbpB strains at permissive...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.1.19-25.1987
更新日期:1987-01-01 00:00:00
abstract::A selection procedure is described which permits a large number of Saccharomyces cerevisiae mutants to be screened for specific lesions in mitochondrial respiratory enzymes and the adenosine triphosphatase. The method has been used to isolate nuclear mutant strains with specific lesions in coenzyme QH2-cytochrome c re...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.122.3.826-831.1975
更新日期:1975-06-01 00:00:00
abstract::The importance of the physiological state of a culture of Saccharomyces cerevisiae for tolerance to sudden osmotic dehydration was studied, and it was investigated whether specific osmotolerance factors were demonstrable. The microcalorimeter was used to monitor growth, and different physiological states of the cultur...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.10.4562-4568.1988
更新日期:1988-10-01 00:00:00
abstract:UNLABELLED:Bacteriophage λ N protein binds boxB RNA hairpins in the nut (N utilization) sites of immediate early λ transcripts and interacts with host factors to suppress transcriptional termination at downstream terminators. In opposition to λ N, the Nun protein of HK022 binds the boxBs of coinfecting λ transcripts, i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00466-15
更新日期:2015-11-01 00:00:00
abstract::The galP1 promoter is responsible for galactose-dependent, glucose-sensitive transcription of the galactose utilization operon of Streptomyces coelicolor and Streptomyces lividans. We describe the characterization of mutations that were positioned directly upstream of the apparent transcription start site of galP1 and...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.5.1213-1220.1993
更新日期:1993-03-01 00:00:00
abstract::We have developed a method for efficiently generating transient pores in the outer membranes of Escherichia coli K-12 derivatives by using a new type of electroporation apparatus. The pores are large enough and persist long enough to facilitate the equilibration of plasmid molecules between the intracellular and extra...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.6.2796-2801.1988
更新日期:1988-06-01 00:00:00
abstract::Streptococcus mutans is a key contributor to the formation of the extracellular polysaccharide (EPS) matrix in dental biofilms. The exopolysaccharides, which are mostly glucans synthesized by streptococcal glucosyltransferases (Gtfs), provide binding sites that promote accumulation of microorganisms on the tooth surfa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01649-09
更新日期:2010-06-01 00:00:00
abstract::We present the nucleotide sequence of the oriT region from plasmid R100. Comparison to other IncF plasmids revealed homology around the proposed nick sites as well as conservation of inverted repeated sequences in the nonhomologous region. Three areas showed strong homology (eight of nine nucleotides) to the consensus...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.8.3829-3832.1987
更新日期:1987-08-01 00:00:00
abstract::Escherichia coli O111a is a thermosensitive strain which, when grown at 40 C, accumulates large quantities of intracellular membranes. The ultrastructure of these membranes in cells which have been chemically fixed, embedded, and examined as thin sections has been compared with that of membranes in cells negatively st...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.113.1.433-444.1973
更新日期:1973-01-01 00:00:00