Abstract:
:The rice pathogen recognition receptor, XA21, confers resistance to Xanthomonas oryzae pv. oryzae strains producing the type one system-secreted molecule, AvrXA21. X. oryzae pv. oryzae requires a regulatory two-component system (TCS) called RaxRH to regulate expression of eight rax (required for AvrXA21 activity) genes and to sense population cell density. To identify other key components in this critical regulatory circuit, we assayed proteins expressed in a raxR gene knockout strain. This survey led to the identification of the phoP gene encoding a response regulator that is up-regulated in the raxR knockout strain. Next we generated a phoP knockout strain and found it to be impaired in X. oryzae pv. oryzae virulence and no longer able to activate the response regulator HrpG (hypersensitive reaction and pathogenicity G) in response to low levels of Ca2+. The impaired virulence of the phoP knockout strain can be partially complemented by constitutive expression of hrpG, indicating that PhoP controls a key aspect of X. oryzae pv. oryzae virulence through regulation of hrpG. A gene encoding the cognate putative histidine protein kinase, phoQ, was also isolated. Growth curve analysis revealed that AvrXA21 activity is impaired in a phoQ knockout strain as reflected by enhanced growth of this strain in rice lines carrying XA21. These results suggest that the X. oryzae pv. oryzae PhoPQ TCS functions in virulence and in the production of AvrXA21 in partnership with RaxRH.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Lee SW,Jeong KS,Han SW,Lee SE,Phee BK,Hahn TR,Ronald Pdoi
10.1128/JB.01406-07subject
Has Abstractpub_date
2008-03-01 00:00:00pages
2183-97issue
6eissn
0021-9193issn
1098-5530pii
JB.01406-07journal_volume
190pub_type
杂志文章abstract::Evidence is reported here that alkalinization of Clostridium acetobutylicum cytoplasm involves hydrogenase activity. A decrease of in vivo hydrogenase activity is accompanied by intracellular accumulation of protons leading to a negative (interior acidic) pH gradient. However, the organism is able to maintain a consta...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.19.6146-6147.1994
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journal_title:Journal of bacteriology
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doi:10.1128/jb.176.11.3102-3110.1994
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journal_title:Journal of bacteriology
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doi:10.1128/JB.139.3.1001-1006.1979
更新日期:1979-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2002-06-01 00:00:00
abstract::Alginate-producing (mucoid) strains of Pseudomonas aeruginosa possess a 54-kDa outer membrane (OM) protein (AlgE) which is missing in nonmucoid bacteria. The coding region of the algE gene from mucoid P. aeruginosa CF3/M1 was subcloned in the expression vector pT7-7 and expressed in Escherichia coli. The level of expr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.18.5639-5647.1994
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更新日期:1993-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.111.2.481-487.1972
更新日期:1972-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.5.1977-1985.1966
更新日期:1966-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.155.3.1434-1438.1983
更新日期:1983-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1964-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.186.11.3355-3362.2004
更新日期:2004-06-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1986-07-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1976-10-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1975-08-01 00:00:00
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更新日期:1998-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1972-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.3.723-731.1993
更新日期:1993-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.165.3.837-842.1986
更新日期:1986-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2001-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.16.4601-4611.2002
更新日期:2002-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2009-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.4.2124-2127.1989
更新日期:1989-04-01 00:00:00
abstract::We have developed a rapid and sensitive fluorimetric method, based on the formation of a fluorescent product from nitrosation of 2,3-diaminonaphthalene, for measuring the ability of bacteria to catalyze nitrosation of amines. We have shown in Escherichia coli that nitrosation can be induced under anaerobic conditions ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.1.359-364.1988
更新日期:1988-01-01 00:00:00