Abstract:
:We are currently investigating the role of ToxR-mediated gene regulation in Photobacterium profundum strain SS9. SS9 is a moderately piezophilic ("pressure loving") psychrotolerant marine bacterium belonging to the family Vibrionaceae. In Vibrio cholerae, ToxR is a transmembrane DNA binding protein involved in mediating virulence gene expression in response to various environmental signals. A homolog to V. cholerae ToxR that is necessary for pressure-responsive gene expression of two outer membrane protein-encoding genes was previously found in SS9. To search for additional genes regulated by ToxR in SS9, we have used RNA arbitrarily primed PCR (RAP-PCR) with wild-type and toxR mutant strains of SS9. Seven ToxR-activated transcripts and one ToxR-repressed transcript were identified in this analysis. The cDNAs corresponding to these partial transcripts were cloned and sequenced, and ToxR regulation of their genes was verified. The products of these genes are all predicted to fall into one or both of two functional categories, those whose products alter membrane structure and/or those that are part of a starvation response. The transcript levels of all eight newly identified genes were also characterized as a function of hydrostatic pressure. Various patterns of pressure regulation were observed, indicating that ToxR activation or repression cannot be used to predict the influence of pressure on gene expression in SS9. These results provide further information on the nature of the ToxR regulon in SS9 and indicate that RAP-PCR is a useful approach for the discovery of new genes under the control of global regulatory transcription factors.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Bidle KA,Bartlett DHdoi
10.1128/JB.183.5.1688-1693.2001keywords:
subject
Has Abstractpub_date
2001-03-01 00:00:00pages
1688-93issue
5eissn
0021-9193issn
1098-5530journal_volume
183pub_type
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journal_title:Journal of bacteriology
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doi:10.1128/jb.182.24.6913-6920.2000
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journal_title:Journal of bacteriology
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更新日期:1989-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1994-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:1978-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.94.1.61-65.1967
更新日期:1967-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.1.20-26.1962
更新日期:1962-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.117.2.775-782.1974
更新日期:1974-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.9.2963-2968.2001
更新日期:2001-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.130.1.1-3.1977
更新日期:1977-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1996-08-01 00:00:00
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更新日期:1974-02-01 00:00:00
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更新日期:1967-01-01 00:00:00
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更新日期:2002-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2006-04-01 00:00:00
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doi:10.1128/jb.176.12.3500-3507.1994
更新日期:1994-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.8.2469-2470.1996
更新日期:1996-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.155.2.512-521.1983
更新日期:1983-08-01 00:00:00
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更新日期:2014-02-01 00:00:00
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更新日期:1984-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.134.2.629-635.1978
更新日期:1978-05-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:2003-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2000-08-01 00:00:00