Subcellular sites involved in lipid synthesis in Saccharomyces cerevisiae.

abstract：:A novel genetic scheme allowed us to isolate extragenic suppressor mutations that restored mutant OmpF assembly. One group of these mutations, termed asmB for assembly suppressor mutation B, permitted mutant OmpF assembly in a non-allele-specific manner. Genetic mapping analyses placed the asmB mutations at the 2-min ...

journal_title：Journal of bacteriology

authors： Kloser AW,Laird MW,Misra R

更新日期：1996-09-01 00:00:00

abstract：UNLABELLED:Bacterial cell division is an essential and highly coordinated process. It requires the polymerization of the tubulin homologue FtsZ to form a dynamic ring (Z-ring) at midcell. Z-ring formation relies on a group of FtsZ-associated proteins (Zap) for stability throughout the process of division. In Escherichi...

journal_title：Journal of bacteriology

authors： Roach EJ,Wroblewski C,Seidel L,Berezuk AM,Brewer D,Kimber MS,Khursigara CM

更新日期：2016-05-13 00:00:00

abstract：:Bacteriophage lambda adsorbs to its Escherichia coli K-12 host by interacting with LamB, its cell-surface receptor. We fused C-terminal portions of J, the tail fiber protein of lambda, to maltose-binding protein. Solid-phase binding assays demonstrated that a purified fusion protein comprising only the last 249 residu...

journal_title：Journal of bacteriology

authors： Wang J,Hofnung M,Charbit A

更新日期：2000-01-01 00:00:00

abstract：:The ompB operon encodes OmpR and EnvZ, two proteins that are necessary for the expression and osmoregulation of the OmpF and OmpC porins in Escherichia coli. We have used in vitro and in vivo experiments to show that cyclic AMP and the cyclic AMP receptor protein (CRP) directly regulate ompB. ompB expression in an omp...

journal_title：Journal of bacteriology

authors： Huang L,Tsui P,Freundlich M

更新日期：1992-02-01 00:00:00

abstract：:We have constructed a suicide vector, pU1800, containing the transposable element TnphoA (Tn5 IS50L::phoA), for the purpose of producing protein fusions in vivo between the Escherichia coli alkaline phosphatase (APase) and proteins of the facultative photoheterotroph, Rhodobacter sphaeroides. We introduced TnphoA into...

journal_title：Journal of bacteriology

authors： Moore MD,Kaplan S

更新日期：1989-08-01 00:00:00

abstract：:Penicillin-binding proteins of 180, 89, 80, 68, 61, 41, and 38 kilodaltons were identified in Treponema pallidum (Nichols) by their covalent binding of [35S]benzylpenicillin. Penicillin-binding proteins are localized in the plasma membranes of many bacterial species and may serve as useful markers for determining plas...

journal_title：Journal of bacteriology

authors： Cunningham TM,Miller JN,Lovett MA

更新日期：1987-11-01 00:00:00

abstract：:The glmU gene product of Escherichia coli was recently identified as the N-acetylglucosamine-1-phosphate uridyltransferase activity which catalyzes the formation of UDP-N-acetylglucosamine, an essential precursor for cell wall peptidoglycan and lipopolysaccharide biosyntheses (D. Mengin-Lecreulx and J. van Heijenoort,...

journal_title：Journal of bacteriology

authors： Mengin-Lecreulx D,van Heijenoort J

更新日期：1994-09-01 00:00:00

abstract：:Two clindamycin-erythromycin resistance transfer factors of Bacteroides fragilis, pBF4 and pBFTM10, were analyzed for regions of DNA homology. Although the plasmids were derived from different clinical isolates of B. fragilis and have different sizes, they showed homology in the clindamycin-erythromycin resistance reg...

journal_title：Journal of bacteriology

authors： Shimell MJ,Smith CJ,Tally FP,Macrina FL,Malamy MH

更新日期：1982-11-01 00:00:00

abstract：:Although yeast cannot normally incorporate exogenous deoxythymidine 5'-monophosphate (dTMP) into deoxyribonucleic acid, mutants able to do so have been isolated. We have characterized a recessive suppressor of dTMP uptake (sot1) that prevents strains carrying either tup1, tup2, or tup4 from growing on selective medium...

journal_title：Journal of bacteriology

authors： Remer S,Sherman A,Kraig E,Haber JE

更新日期：1979-05-01 00:00:00

abstract：:Bacterial cell division is mediated by a set of proteins that assemble to form a large multiprotein complex called the divisome. Recent studies in Bacillus subtilis and Escherichia coli indicate that cell division proteins are involved in multiple cooperative binding interactions, thus presenting a technical challenge...

journal_title：Journal of bacteriology

authors： Robichon C,King GF,Goehring NW,Beckwith J

更新日期：2008-09-01 00:00:00

abstract：:Autoinducer-2 (AI-2) is required for biofilm formation and virulence of the oral pathogen Aggregatibacter actinomycetemcomitans, and we previously showed that lsrB codes for a receptor for AI-2. The lsrB gene is expressed as part of the lsrACDBFG operon, which is divergently transcribed from an adjacent lsrRK operon. ...

journal_title：Journal of bacteriology

authors： Torres-Escobar A,Juárez-Rodríguez MD,Lamont RJ,Demuth DR

更新日期：2013-01-01 00:00:00

abstract：:The SOS response to DNA damage in bacteria is a well-known component of the complex transcriptional responses to genotoxic environmental stresses such as exposure to reactive oxygen species, alkylating agents, and many of the antibiotics targeting DNA replication. However, bacteria such as Bacillus subtilis also respo...

journal_title：Journal of bacteriology

authors： Hoover SE,Xu W,Xiao W,Burkholder WF

更新日期：2010-08-01 00:00:00

abstract：:Diploid cells of Saccharomyces cerevisiae underwent meiosis and sporulation when placed in 1% potassium acetate sporulation medium. In unbuffered sporulation medium the pH rose very rapidly, reaching pH 8.4 after 2 h of sporulation. Under these conditions, the uptake of radioactive adenine and lysine was extremely lim...

journal_title：Journal of bacteriology

authors： McCusker JH,Haber JE

更新日期：1977-10-01 00:00:00

abstract：:We have cloned the Micromonospora viridifaciens neuraminidase (EC 3.2.1.18) gene (nedA) in Streptomyces lividans. This was accomplished by using the vector pIJ702 and BglII-BclI libraries of M. viridifaciens chromosomal inserts created in S. lividans. The libraries were screened for the expression of neuraminidase by ...

journal_title：Journal of bacteriology

authors： Sakurada K,Ohta T,Hasegawa M

更新日期：1992-11-01 00:00:00

abstract：:RpoS, the sigma factor of enteric bacteria that responds to stress and stationary phase, is subject to complex regulation acting at multiple levels, including transcription, translation, and proteolysis. Increased translation of rpoS mRNA during growth at low temperature, after osmotic challenge, or with a constitutiv...

journal_title：Journal of bacteriology

authors： Jones AM,Goodwill AG,Elliott T

更新日期：2006-07-01 00:00:00

abstract：:Transcriptome analysis of a Bacillus subtilis rok strain that showed reduced complex colony structure formation revealed significant downregulation of the yuaB gene. Overexpression of yuaB restored structure formation in the rok strain. We show that transcription of yuaB is indirectly regulated by Rok, independently f...

journal_title：Journal of bacteriology

authors： Kovács AT,Kuipers OP

更新日期：2011-02-01 00:00:00

abstract：:Yersinia enterocolitica transports YscM1 and YscM2 via the type III pathway, a mechanism that is required for the establishment of bacterial infections. Prior to host cell contact, YscM1 and YscM2 exert posttranscriptional regulation to inhibit expression of effector yop genes, which encode virulence factors that trav...

journal_title：Journal of bacteriology

authors： Cambronne ED,Sorg JA,Schneewind O

更新日期：2004-02-01 00:00:00

abstract：:No catalase activity was detected in four strains of glucose-grown Mycoplasma pneumoniae at any time during the replication of the organism. Exogenous catalase dramatically increased the O(2) uptake with glycerol, presumably by releasing inhibition caused by hydrogen peroxide. The effect of added catalase on the O(2) ...

journal_title：Journal of bacteriology

authors： Low IE,Eaton MD,Proctor P

更新日期：1968-04-01 00:00:00

abstract：:Halobacterium halobium spontaneously reverses its swimming direction with a frequency of about 0.1 s-1. The self-sustained periodicity can be entrained by rhythmic light stimuli within a limited range between 0.13 and 0.3 s-1. Increase of temperature increases the reversal frequency and shortens the time to reach maxi...

journal_title：Journal of bacteriology

authors： Schimz A,Hildebrand E

更新日期：1986-05-01 00:00:00

abstract：:The morphological and physiological characteristics of 20 isolates of psychrophilic Bacillus were compared with 29 strains representing nine species of mesophilic Bacillus and 2 strains of Sporosarcina ureae to determine the taxonomic position of the psychrophiles. The psychrophiles formed four distinct groups which w...

journal_title：Journal of bacteriology

authors： Larkin JM,Stokes JL

更新日期：1967-10-01 00:00:00

abstract：:An assay was developed to determine the amount of cyanophycin granules in blue-green algae. The amount of this polypeptide in cells of Anabaena cylindrica was measured as a function of culture age and was compared with changes in other proteinaceous cellular components. The data presented support the notion that the c...

journal_title：Journal of bacteriology

authors： Simon RD

更新日期：1973-06-01 00:00:00

abstract：:Six mutants, allelic to ade3, were isolated after mutagenic treatment of a prototrophic strain of yeast. All six grow on medium supplemented with adenine alone and four respond to histidine. Supplementation with adenine plus histidine or methionine inhibits growth, but a mixture of these three is stimulatory. Heteroal...

journal_title：Journal of bacteriology

authors： Lomax CA,Gross TS,Woods RA

更新日期：1971-07-01 00:00:00

abstract：:Stalk formation is a novel pattern of multicellular organization. Yeast cells which survive UV irradiation form colonies that grow vertically to form very long (0.5 to 3.0 cm) and thin (0.5 to 4 mm in diameter) multicellular structures. We describe the conditions required to obtain these stalk-like structures reproduc...

journal_title：Journal of bacteriology

authors： Engelberg D,Mimran A,Martinetto H,Otto J,Simchen G,Karin M,Fink GR

更新日期：1998-08-01 00:00:00

abstract：:We have identified an analog of SspB, the proteolytic adaptor for transfer-messenger RNA (tmRNA)-tagged proteins, in Caulobacter crescentus. C. crescentus SspB shares limited sequence similarity with Escherichia coli SspB but binds the tmRNA tag in vitro and is required for optimal proteolysis of tagged proteins in vi...

journal_title：Journal of bacteriology

authors： Lessner FH,Venters BJ,Keiler KC

更新日期：2007-01-01 00:00:00

abstract：:Rhizobium phaseoli lacks glutamate dehydrogenase (GDH) and assimilates ammonium by the glutamine synthetase-glutamate synthase pathway. A strain of R. phaseoli harboring the Escherichia coli GDH structural gene (gdhA) was constructed. GDH activity was expressed in R. phaseoli in the free-living state and in symbiosis....

journal_title：Journal of bacteriology

authors： Bravo A,Becerril B,Mora J

更新日期：1988-02-01 00:00:00

abstract：:Two-micrometer deoxyribonucleic acid (DNA), a circular plasmid of Saccharomyces cerevisiae, contains two nontandem repeated sequences which are inverted with respect to one another. These repeated sequences together account for 21% of the molecule length. Restriction endonuclease analysis and electron microscopy demon...

journal_title：Journal of bacteriology

authors： Livingston DM,Klein HL

更新日期：1977-01-01 00:00:00

abstract：:An enzymatic assay was developed to measure tetrahydromethanopterin (H(4)MPT) levels in wild-type and mutant cells of Methylobacterium extorquens AM1. H(4)MPT was detectable in wild-type cells but not in strains with a mutation of either the orf4 or the dmrA gene, suggesting a role for these two genes in H(4)MPT biosy...

journal_title：Journal of bacteriology

authors： Rasche ME,Havemann SA,Rosenzvaig M

更新日期：2004-03-01 00:00:00

abstract：:The pED208 plasmid is a 90-kilobase conjugative plasmid which is the derepressed form of Fo lac plasmid (IncFV). A 3.3-kilobase HindIII-PstI fragment from the pED208 plasmid was cloned and sequenced and was found to contain four open reading frames which were highly homologous to the traA, traL, traE, and traY gene pr...

journal_title：Journal of bacteriology

authors： Finlay BB,Frost LS,Paranchych W

更新日期：1986-11-01 00:00:00

abstract：:Although 95% of the enterotoxin B produced by Staphylococcus aureus appears during the latter part of the exponential phase of growth, growth per se is not necessary for toxin synthesis. A procedure is described whereby a concentrated suspension (at least 6 x 10(10) cells per ml) of a 16-hr culture of S. aureus was fo...

journal_title：Journal of bacteriology

authors： Markus Z,Silverman GJ

更新日期：1969-02-01 00:00:00

abstract：:Screening of derivatives of Rhizobium etli KIM5s randomly mutagenized with mTn5SSgusA30 resulted in the identification of strain KIM-G1. Its rough colony appearance, flocculation in liquid culture, and Ndv(-) Fix(-) phenotype were indicative of a lipopolysaccharide (LPS) defect. Electrophoretic analysis of cell-associ...

journal_title：Journal of bacteriology

authors： Vinuesa P,Reuhs BL,Breton C,Werner D

更新日期：1999-09-01 00:00:00