DIFFERENTIATION BY IMMUNODIFFUSION AND BY QUANTITATIVE IMMUNOFLUORESCENCE BETWEEN 5-FLUOROURACIL-TREATED AND NORMAL CELLS FROM A TOXINOGENIC STAPHYLOCOCCUS AUREUS STRAIN.

Abstract:

:De Repentigny, J. (University of Montreal, Montreal, Quebec, Canada), S. Sonea, and A. Frappier. Differentiation by immunodiffusion and by quantitative immunofluorescence between 5-fluorouracil-treated and normal cells from a toxinogenic Staphylococcus aureus strain. J. Bacteriol. 88:444-448. 1964.-Immunodiffusion and quantitative immunofluorescence can both detect antigenic changes produced by 5-fluorouracil (FU) in Staphylococcus aureus Wood 46 strain. When FU is added to the cultures in their logarithmic phase of growth, a number of bacterial antigens are no longer detectable by immuno-diffusion and the intensity of the total immuno-fluorescence of bacteria is diminished; thus, these antigens are either profoundly modified or no longer synthesized. Uracil and, less effectively, thymine can reverse the FU inhibitory effect on the synthesis of antigens, and the number of precipitin lines remains closer to controls. The immunochemical approach provides a new way of obtaining information on the action of this pyrimidine analogue on metabolic processes in pathogenic bacteria. Microscopic quantitative immunofluorescence seems to be adaptable to give indirect information on changes in the metabolism or synthesis or antigens of a single bacterial cell.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

DEREPENTIGNY J,SONEA S,FRAPPIER A

doi

10.1128/JB.88.2.444-448.1964

keywords:

subject

Has Abstract

pub_date

1964-08-01 00:00:00

pages

444-8

eissn

0021-9193

issn

1098-5530

journal_volume

88

pub_type

杂志文章
  • A Suppressor Mutation That Creates a Faster and More Robust σE Envelope Stress Response.

    abstract:UNLABELLED:The σE envelope stress response is an essential signal transduction pathway which detects and removes mistargeted outer membrane (OM) β-barrel proteins (OMPs) in the periplasm of Escherichia coli It relies on σE, an alternative sigma factor encoded by the rpoE gene. Here we report a novel mutation, a nucleot...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00340-16

    authors: Konovalova A,Schwalm JA,Silhavy TJ

    更新日期:2016-08-11 00:00:00

  • Uptake of N,N'-diacetylchitobiose [(GlcNAc)2] via the phosphotransferase system is essential for chitinase production by Serratia marcescens 2170.

    abstract::The chiR gene of Serratia marcescens 2170, encoding a LysR-type transcriptional activator, was identified previously as an essential factor for expression of chitinases and a chitin-binding protein, CBP21. To identify other genes that are essential for chitinase production, transposon mutagenesis with mini-Tn5Km1 was ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.6.1776-1782.2003

    authors: Uchiyama T,Kaneko R,Yamaguchi J,Inoue A,Yanagida T,Nikaidou N,Regue M,Watanabe T

    更新日期:2003-03-01 00:00:00

  • Mutations in the -10 TATAAT sequence of the gyrA promoter affect both promoter strength and sensitivity to DNA supercoiling.

    abstract::Transcription of the gyrA and gyrB genes, which encode the subunits of DNA gyrase, increases in response to DNA relaxation. Previous studies have shown that a small segment of DNA extending from the -10 consensus hexamer to the start of transcription encodes the sequence determinants for this response. In this study, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.19.5999-6006.1994

    authors: Straney R,Krah R,Menzel R

    更新日期:1994-10-01 00:00:00

  • Influence of a putative ECF sigma factor on expression of the major outer membrane protein, OprF, in Pseudomonas aeruginosa and Pseudomonas fluorescens.

    abstract::The gene encoding OprF, a major outer membrane protein in Pseudomonas species (formerly known as type 1 pseudomonads), was thought to be constitutively transcribed from a single sigma 70 promoter immediately upstream of the gene. We now report the identification of a novel putative ECF (extracytoplasmic function) sigm...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.16.4746-4754.1999

    authors: Brinkman FS,Schoofs G,Hancock RE,De Mot R

    更新日期:1999-08-01 00:00:00

  • Restoration by ubiquinone of Azotobacter vinelandii reduced nicotinamide adenine dinucleotide oxidase activity.

    abstract::Extraction with pentane virtually abolished reduced nicotinamide adenine dinucleotide oxidase activity in small particles from Azotobacter vinelandii, but activity was largely restored by added ubiquinone. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.98.1.311-313.1969

    authors: Swank RT,Burris RH

    更新日期:1969-04-01 00:00:00

  • Dual regulation of the ugp operon by phosphate and carbon starvation at two interspaced promoters.

    abstract::The ugp operon of Escherichia coli includes genes involved in the uptake of sn-glycerol-3-phosphate and glycerophosphoryl diesters and belongs to the pho regulon which is induced by phosphate limitation. This operon has two transcriptional initiation sites, as determined by S1 nuclease mapping of the in vivo transcrip...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.2.549-558.1991

    authors: Kasahara M,Makino K,Amemura M,Nakata A,Shinagawa H

    更新日期:1991-01-01 00:00:00

  • INTRACELLULAR M PROTEIN OF GROUP A STREPTOCOCCUS.

    abstract::Fox, Eugene N. (University of Chicago, Chicago, Ill.). Intracellular M protein of group A Streptococcus. J. Bacteriol. 85:536-540. 1963.-A heat-labile M protein antigen in protoplasts of a type 14 strain of group A Streptococcus has been demonstrated in a soluble form in the cytoplasm, and also bound to the protoplasm...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.85.3.536-540.1963

    authors: FOX EN

    更新日期:1963-03-01 00:00:00

  • Physiological function of superoxide dismutase in glucose-limited chemostat cultures of Escherichia coli.

    abstract::Conditions for continuous culture of Escherichia coli K-12 His- Thi- under glucose limitation were established. Both the capacity for respiration, at D greater than 0.2/h, and specific activity of superoxide dismutase increased as a function of specific growth rate, whereas peroxidase and catalase were either invarian...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.130.2.805-811.1977

    authors: Hassan HM,Fridovich I

    更新日期:1977-05-01 00:00:00

  • Cloning, sequences, and characterization of two chitinase genes from the Antarctic Arthrobacter sp. strain TAD20: isolation and partial characterization of the enzymes.

    abstract::Arthrobacter sp. strain TAD20, a chitinolytic gram-positive organism, was isolated from the sea bottom along the Antarctic ice shell. Arthrobacter sp. strain TAD20 secretes two major chitinases, ChiA and ChiB (ArChiA and ArChiB), in response to chitin induction. A single chromosomal DNA fragment containing the genes c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.5.1773-1779.2001

    authors: Lonhienne T,Mavromatis K,Vorgias CE,Buchon L,Gerday C,Bouriotis V

    更新日期:2001-03-01 00:00:00

  • An amino acid substitution in penicillin-binding protein 3 creates pointed polar caps in Escherichia coli.

    abstract::The pbpB gene product penicillin-binding protein 3 (PBP3) of Escherichia coli is one of the major targets of beta-lactam antibiotics. At the permissive temperature, the temperature-sensitive pbpBr1 mutant, which was obtained after selection for increased resistance to cephalexin, shows a dramatic change in shape which...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.10.4828-4837.1988

    authors: Taschner PE,Ypenburg N,Spratt BG,Woldringh CL

    更新日期:1988-10-01 00:00:00

  • Isolation and characterization of Salmonella typhimurium glyoxylate shunt mutants.

    abstract::Growth of Salmonella typhimurium on acetate as a sole carbon source requires expression of the glyoxylate shunt; however, the genes for the glyoxylate shunt enzymes have not been previously identified in S. typhimurium. In this study, we isolated transposon insertions in the genes for the two unique enzymes of this pa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.7.3029-3034.1987

    authors: Wilson RB,Maloy SR

    更新日期:1987-07-01 00:00:00

  • Penicillin-binding proteins in Borrelia burgdorferi.

    abstract::Penicillin-binding proteins were identified in Borrelia burgdorferi membranes. A 94-kilodalton penicillin-binding protein was the first to be labeled with tritiated penicillin and was the first band to disappear in a competition experiment. Its binding ability was destroyed when membranes were preboiled. In addition, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.10.6139-6141.1990

    authors: Urban C,Rahal JJ,Dattwyller RJ,Gorevic P,Luft BJ

    更新日期:1990-10-01 00:00:00

  • Uncontrolled septation in a cell division cycle mutant of the fission yeast Schizosaccharomyces pombe.

    abstract::A temperature-sensitive Schizosaccharomyces pombe mutant, cdc16-116, has been isolated which undergoes uncontrolled septation during its cell division cycle. The mutant accumulates two types of cells after 3 h of growth at the restrictive temperature: (i) type I cells (85% of the population), which complete nuclear di...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.137.1.440-446.1979

    authors: Minet M,Nurse P,Thuriaux P,Mitchison JM

    更新日期:1979-01-01 00:00:00

  • Identification of genes encoding components of the swarmer cell flagellar motor and propeller and a sigma factor controlling differentiation of Vibrio parahaemolyticus.

    abstract::Vibrio parahaemolyticus possesses two distinct motility systems, the polar system used for swimming in liquid environments and the lateral system used for swarming over surfaces. Growth on surfaces induces swarmer cell differentiation and expression of the lateral motility system. Mutants, created by transposon mutage...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.11.3361-3371.1993

    authors: McCarter LL,Wright ME

    更新日期:1993-06-01 00:00:00

  • Enterotoxin B synthesis by replicating and nonreplicating cells of Staphylococcus aureus.

    abstract::Although 95% of the enterotoxin B produced by Staphylococcus aureus appears during the latter part of the exponential phase of growth, growth per se is not necessary for toxin synthesis. A procedure is described whereby a concentrated suspension (at least 6 x 10(10) cells per ml) of a 16-hr culture of S. aureus was fo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.97.2.506-512.1969

    authors: Markus Z,Silverman GJ

    更新日期:1969-02-01 00:00:00

  • Phosphopantetheine adenylyltransferase from Escherichia coli: investigation of the kinetic mechanism and role in regulation of coenzyme A biosynthesis.

    abstract::Phosphopantetheine adenylyltransferase (PPAT) from Escherichia coli is an essential hexameric enzyme that catalyzes the penultimate step in coenzyme A (CoA) biosynthesis and is a target for antibacterial drug discovery. The enzyme utilizes Mg-ATP and phosphopantetheine (PhP) to generate dephospho-CoA (dPCoA) and pyrop...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00732-07

    authors: Miller JR,Ohren J,Sarver RW,Mueller WT,de Dreu P,Case H,Thanabal V

    更新日期:2007-11-01 00:00:00

  • Two nitrate/nitrite transporters are encoded within the mobilizable plasmid for nitrate respiration of Thermus thermophilus HB8.

    abstract::Thermus thermophilus HB8 can grow anaerobically by using a membrane-bound nitrate reductase to catalyze the reduction of nitrate as a final electron acceptor in respiration. In contrast to other denitrifiers, the nitrite produced does not continue the reduction pathway but accumulates in the growth medium after its ac...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.8.2179-2183.2000

    authors: Ramírez S,Moreno R,Zafra O,Castán P,Vallés C,Berenguer J

    更新日期:2000-04-01 00:00:00

  • Isolation, phenotypic characterization, and complementation analysis of mutants of Methylobacterium extorquens AM1 unable to synthesize pyrroloquinoline quinone and sequences of pqqD, pqqG, and pqqC.

    abstract::Aerobic gram-negative methylotrophs oxidize methanol to formaldehyde by using a methanol dehydrogenase that has pyrroloquinoline quinone (PQQ) as a prosthetic group. Seventy-two mutants which are unable to grow on methanol unless the growth medium is supplemented with PQQ have been isolated in the facultative methanol...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.6.1746-1755.1994

    authors: Morris CJ,Biville F,Turlin E,Lee E,Ellermann K,Fan WH,Ramamoorthi R,Springer AL,Lidstrom ME

    更新日期:1994-03-01 00:00:00

  • Targeting the Achilles' heel of bacteria: different mechanisms to break down the peptidoglycan cell wall during bacterial warfare.

    abstract::Bacteria commonly live in dense polymicrobial communities and compete for scarce resources. Consequently, they employ a diverse array of mechanisms to harm, inhibit and kill their competitors. The cell wall is essential for bacterial survival by providing mechanical strength to resist osmotic stress. Because peptidogl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章,评审

    doi:10.1128/JB.00478-20

    authors: Sibinelli-Sousa S,Hespanhol JT,Bayer-Santos E

    更新日期:2020-11-02 00:00:00

  • Origin and fate of the 3' ends of single-stranded DNA generated by conjugal transfer of plasmid R1162.

    abstract::During conjugation, a single strand of DNA is cleaved at the origin of transfer (oriT) by the plasmid-encoded relaxase. This strand is then unwound from its complement and transferred in the 5'-to-3' direction, with the 3' end likely extended by rolling-circle replication. The resulting, newly synthesized oriT must th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00818-12

    authors: Becker EC,Meyer R

    更新日期:2012-10-01 00:00:00

  • EFFECT OF HIGH FAT DIETS ON INTESTINAL MICROFLORA AND SERUM CHOLESTEROL IN RATS.

    abstract::Graber, C. D. (Baylor University College of Medicine, Houston, Tex.), R. M. O'Neal, and E. R. Rabin. Effect of high fat diets on intestinal microflora and serum cholesterol in rats. J. Bacteriol. 89:47-51. 1965.-Differential bacterial counts of feces and total plasma cholesterol determinations were performed on 60 Wis...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.89.1.47-51.1965

    authors: GRABER CD,O'NEAL RM,RABIN ER

    更新日期:1965-01-01 00:00:00

  • Segmental message stabilization as a mechanism for differential expression from the Zymomonas mobilis gap operon.

    abstract::In Zymomonas mobilis, three- to fourfold more glyceraldehyde-3-phosphate dehydrogenase protein than phosphoglycerate kinase is needed for glycolysis because of differences in catalytic efficiency. Consistent with this requirement, higher levels of glyceraldehyde-3-phosphate dehydrogenase were observed with two-dimensi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.1.245-254.1991

    authors: Eddy CK,Keshav KF,An H,Utt EA,Mejia JP,Ingram LO

    更新日期:1991-01-01 00:00:00

  • UDP-glucuronic acid decarboxylases of Bacteroides fragilis and their prevalence in bacteria.

    abstract::Xylose is rarely described as a component of bacterial glycans. UDP-xylose is the nucleotide-activated form necessary for incorporation of xylose into glycans and is synthesized by the decarboxylation of UDP-glucuronic acid (UDP-GlcA). Enzymes with UDP-GlcA decarboxylase activity include those that lead to the formati...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05337-11

    authors: Coyne MJ,Fletcher CM,Reinap B,Comstock LE

    更新日期:2011-10-01 00:00:00

  • Bacterial plasmids that carry two functional centromere analogs are stable and are partitioned faithfully.

    abstract::The par genes of unit-copy plasmids P1 and F promote equitable distribution of plasmid copies to daughter cells and can be considered to be functional analogs of eucaryotic centromeres. Composite plasmids were constructed which carry either two functional P1 par regions or one F and one P1 region. Unlike dicentric chr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.158.2.742-745.1984

    authors: Austin SJ

    更新日期:1984-05-01 00:00:00

  • Isolation and characterization of the lacA gene encoding beta-galactosidase in Bacillus subtilis and a regulator gene, lacR.

    abstract::We have isolated transposon insertions in the lacA gene encoding an endogenous beta-galactosidase of Bacillus subtilis. Upstream of the putative operon containing lacA is a negative regulator, lacR, which encodes a product related to a family of regulators that includes the lactose repressor, lacI, of Escherichia coli...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.17.5636-5638.1997

    authors: Daniel RA,Haiech J,Denizot F,Errington J

    更新日期:1997-09-01 00:00:00

  • Control of chromosome replication in thymine-requiring strains of Bacillus subtilis 168.

    abstract::Study of the replication pattern of a number of B. subtilis 168 strains under controlled physiological conditions revealed great interstrain variation in control of replication. Replication patterns were calculated from ratios of purA16/leu-8 and purA16/metB5 transformation frequency. The thymine-independent strains a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.123.3.1055-1067.1975

    authors: Gillin FD,Ganesan AT

    更新日期:1975-09-01 00:00:00

  • Loss of the penicillinase plasmid after treatment of Staphylococcus aureus with sodium dodecyl sulfate.

    abstract::Two strains of Staphylococcus aureus (PC1 and 196E), when grown in medium containing 0.002% sodium dodecyl sulfate (SDS), showed from 96.1 to 100% loss of the ability to produce penicillinase. Resistance to cadmium and zinc was lost concomitantly with the ability to produce penicillinase. A comparison of the rate of c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.109.1.262-265.1972

    authors: Sonstein SA,Baldwin JN

    更新日期:1972-01-01 00:00:00

  • Flexible Hinges in Bacterial Chemoreceptors.

    abstract::Transmembrane bacterial chemoreceptors are extended, rod-shaped homodimers with ligand-binding sites at one end and interaction sites for signaling complex formation and histidine kinase control at the other. There are atomic-resolution structures of chemoreceptor fragments but not of intact, membrane-inserted recepto...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00593-17

    authors: Akkaladevi N,Bunyak F,Stalla D,White TA,Hazelbauer GL

    更新日期:2018-02-07 00:00:00

  • The amino acid sequence of Lrp is highly conserved in four enteric microorganisms.

    abstract::Lrp (leucine-responsive regulatory protein) is a global regulator of metabolism in Escherichia coli (J. M. Calvo and R. G. Matthews, Microbiol. Rev. 58:466-490, 1994). The lrp genes from three other enteric microorganisms, Enterobacter aerogenes, Klebsiella aerogenes, and Salmonella typhimurium, were cloned and sequen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.6.1624-1626.1995

    authors: Friedberg D,Platko JV,Tyler B,Calvo JM

    更新日期:1995-03-01 00:00:00

  • Isolation and characterization of the DNA region encoding nodulation functions in Bradyrhizobium japonicum.

    abstract::The DNA region encoding early nodulation functions of Bradyrhizobium japonicum 3I1b110 (I110) was isolated by its homology to the functionally similar region from Rhizobium meliloti. Isolation of a number of overlapping recombinant clones from this region allowed the construction of a restriction map of the region. Th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.164.3.1301-1308.1985

    authors: Russell P,Schell MG,Nelson KK,Halverson LJ,Sirotkin KM,Stacey G

    更新日期:1985-12-01 00:00:00