Abstract:
:De Repentigny, J. (University of Montreal, Montreal, Quebec, Canada), S. Sonea, and A. Frappier. Differentiation by immunodiffusion and by quantitative immunofluorescence between 5-fluorouracil-treated and normal cells from a toxinogenic Staphylococcus aureus strain. J. Bacteriol. 88:444-448. 1964.-Immunodiffusion and quantitative immunofluorescence can both detect antigenic changes produced by 5-fluorouracil (FU) in Staphylococcus aureus Wood 46 strain. When FU is added to the cultures in their logarithmic phase of growth, a number of bacterial antigens are no longer detectable by immuno-diffusion and the intensity of the total immuno-fluorescence of bacteria is diminished; thus, these antigens are either profoundly modified or no longer synthesized. Uracil and, less effectively, thymine can reverse the FU inhibitory effect on the synthesis of antigens, and the number of precipitin lines remains closer to controls. The immunochemical approach provides a new way of obtaining information on the action of this pyrimidine analogue on metabolic processes in pathogenic bacteria. Microscopic quantitative immunofluorescence seems to be adaptable to give indirect information on changes in the metabolism or synthesis or antigens of a single bacterial cell.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
DEREPENTIGNY J,SONEA S,FRAPPIER Adoi
10.1128/JB.88.2.444-448.1964keywords:
subject
Has Abstractpub_date
1964-08-01 00:00:00pages
444-8eissn
0021-9193issn
1098-5530journal_volume
88pub_type
杂志文章abstract:UNLABELLED:The σE envelope stress response is an essential signal transduction pathway which detects and removes mistargeted outer membrane (OM) β-barrel proteins (OMPs) in the periplasm of Escherichia coli It relies on σE, an alternative sigma factor encoded by the rpoE gene. Here we report a novel mutation, a nucleot...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00340-16
更新日期:2016-08-11 00:00:00
abstract::The chiR gene of Serratia marcescens 2170, encoding a LysR-type transcriptional activator, was identified previously as an essential factor for expression of chitinases and a chitin-binding protein, CBP21. To identify other genes that are essential for chitinase production, transposon mutagenesis with mini-Tn5Km1 was ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.6.1776-1782.2003
更新日期:2003-03-01 00:00:00
abstract::Transcription of the gyrA and gyrB genes, which encode the subunits of DNA gyrase, increases in response to DNA relaxation. Previous studies have shown that a small segment of DNA extending from the -10 consensus hexamer to the start of transcription encodes the sequence determinants for this response. In this study, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.19.5999-6006.1994
更新日期:1994-10-01 00:00:00
abstract::The gene encoding OprF, a major outer membrane protein in Pseudomonas species (formerly known as type 1 pseudomonads), was thought to be constitutively transcribed from a single sigma 70 promoter immediately upstream of the gene. We now report the identification of a novel putative ECF (extracytoplasmic function) sigm...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.16.4746-4754.1999
更新日期:1999-08-01 00:00:00
abstract::Extraction with pentane virtually abolished reduced nicotinamide adenine dinucleotide oxidase activity in small particles from Azotobacter vinelandii, but activity was largely restored by added ubiquinone. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.98.1.311-313.1969
更新日期:1969-04-01 00:00:00
abstract::The ugp operon of Escherichia coli includes genes involved in the uptake of sn-glycerol-3-phosphate and glycerophosphoryl diesters and belongs to the pho regulon which is induced by phosphate limitation. This operon has two transcriptional initiation sites, as determined by S1 nuclease mapping of the in vivo transcrip...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.2.549-558.1991
更新日期:1991-01-01 00:00:00
abstract::Fox, Eugene N. (University of Chicago, Chicago, Ill.). Intracellular M protein of group A Streptococcus. J. Bacteriol. 85:536-540. 1963.-A heat-labile M protein antigen in protoplasts of a type 14 strain of group A Streptococcus has been demonstrated in a soluble form in the cytoplasm, and also bound to the protoplasm...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.85.3.536-540.1963
更新日期:1963-03-01 00:00:00
abstract::Conditions for continuous culture of Escherichia coli K-12 His- Thi- under glucose limitation were established. Both the capacity for respiration, at D greater than 0.2/h, and specific activity of superoxide dismutase increased as a function of specific growth rate, whereas peroxidase and catalase were either invarian...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.130.2.805-811.1977
更新日期:1977-05-01 00:00:00
abstract::Arthrobacter sp. strain TAD20, a chitinolytic gram-positive organism, was isolated from the sea bottom along the Antarctic ice shell. Arthrobacter sp. strain TAD20 secretes two major chitinases, ChiA and ChiB (ArChiA and ArChiB), in response to chitin induction. A single chromosomal DNA fragment containing the genes c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.5.1773-1779.2001
更新日期:2001-03-01 00:00:00
abstract::The pbpB gene product penicillin-binding protein 3 (PBP3) of Escherichia coli is one of the major targets of beta-lactam antibiotics. At the permissive temperature, the temperature-sensitive pbpBr1 mutant, which was obtained after selection for increased resistance to cephalexin, shows a dramatic change in shape which...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.10.4828-4837.1988
更新日期:1988-10-01 00:00:00
abstract::Growth of Salmonella typhimurium on acetate as a sole carbon source requires expression of the glyoxylate shunt; however, the genes for the glyoxylate shunt enzymes have not been previously identified in S. typhimurium. In this study, we isolated transposon insertions in the genes for the two unique enzymes of this pa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.7.3029-3034.1987
更新日期:1987-07-01 00:00:00
abstract::Penicillin-binding proteins were identified in Borrelia burgdorferi membranes. A 94-kilodalton penicillin-binding protein was the first to be labeled with tritiated penicillin and was the first band to disappear in a competition experiment. Its binding ability was destroyed when membranes were preboiled. In addition, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.10.6139-6141.1990
更新日期:1990-10-01 00:00:00
abstract::A temperature-sensitive Schizosaccharomyces pombe mutant, cdc16-116, has been isolated which undergoes uncontrolled septation during its cell division cycle. The mutant accumulates two types of cells after 3 h of growth at the restrictive temperature: (i) type I cells (85% of the population), which complete nuclear di...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.137.1.440-446.1979
更新日期:1979-01-01 00:00:00
abstract::Vibrio parahaemolyticus possesses two distinct motility systems, the polar system used for swimming in liquid environments and the lateral system used for swarming over surfaces. Growth on surfaces induces swarmer cell differentiation and expression of the lateral motility system. Mutants, created by transposon mutage...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.11.3361-3371.1993
更新日期:1993-06-01 00:00:00
abstract::Although 95% of the enterotoxin B produced by Staphylococcus aureus appears during the latter part of the exponential phase of growth, growth per se is not necessary for toxin synthesis. A procedure is described whereby a concentrated suspension (at least 6 x 10(10) cells per ml) of a 16-hr culture of S. aureus was fo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.97.2.506-512.1969
更新日期:1969-02-01 00:00:00
abstract::Phosphopantetheine adenylyltransferase (PPAT) from Escherichia coli is an essential hexameric enzyme that catalyzes the penultimate step in coenzyme A (CoA) biosynthesis and is a target for antibacterial drug discovery. The enzyme utilizes Mg-ATP and phosphopantetheine (PhP) to generate dephospho-CoA (dPCoA) and pyrop...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00732-07
更新日期:2007-11-01 00:00:00
abstract::Thermus thermophilus HB8 can grow anaerobically by using a membrane-bound nitrate reductase to catalyze the reduction of nitrate as a final electron acceptor in respiration. In contrast to other denitrifiers, the nitrite produced does not continue the reduction pathway but accumulates in the growth medium after its ac...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.8.2179-2183.2000
更新日期:2000-04-01 00:00:00
abstract::Aerobic gram-negative methylotrophs oxidize methanol to formaldehyde by using a methanol dehydrogenase that has pyrroloquinoline quinone (PQQ) as a prosthetic group. Seventy-two mutants which are unable to grow on methanol unless the growth medium is supplemented with PQQ have been isolated in the facultative methanol...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.6.1746-1755.1994
更新日期:1994-03-01 00:00:00
abstract::Bacteria commonly live in dense polymicrobial communities and compete for scarce resources. Consequently, they employ a diverse array of mechanisms to harm, inhibit and kill their competitors. The cell wall is essential for bacterial survival by providing mechanical strength to resist osmotic stress. Because peptidogl...
journal_title:Journal of bacteriology
pub_type: 杂志文章,评审
doi:10.1128/JB.00478-20
更新日期:2020-11-02 00:00:00
abstract::During conjugation, a single strand of DNA is cleaved at the origin of transfer (oriT) by the plasmid-encoded relaxase. This strand is then unwound from its complement and transferred in the 5'-to-3' direction, with the 3' end likely extended by rolling-circle replication. The resulting, newly synthesized oriT must th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00818-12
更新日期:2012-10-01 00:00:00
abstract::Graber, C. D. (Baylor University College of Medicine, Houston, Tex.), R. M. O'Neal, and E. R. Rabin. Effect of high fat diets on intestinal microflora and serum cholesterol in rats. J. Bacteriol. 89:47-51. 1965.-Differential bacterial counts of feces and total plasma cholesterol determinations were performed on 60 Wis...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.1.47-51.1965
更新日期:1965-01-01 00:00:00
abstract::In Zymomonas mobilis, three- to fourfold more glyceraldehyde-3-phosphate dehydrogenase protein than phosphoglycerate kinase is needed for glycolysis because of differences in catalytic efficiency. Consistent with this requirement, higher levels of glyceraldehyde-3-phosphate dehydrogenase were observed with two-dimensi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.1.245-254.1991
更新日期:1991-01-01 00:00:00
abstract::Xylose is rarely described as a component of bacterial glycans. UDP-xylose is the nucleotide-activated form necessary for incorporation of xylose into glycans and is synthesized by the decarboxylation of UDP-glucuronic acid (UDP-GlcA). Enzymes with UDP-GlcA decarboxylase activity include those that lead to the formati...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05337-11
更新日期:2011-10-01 00:00:00
abstract::The par genes of unit-copy plasmids P1 and F promote equitable distribution of plasmid copies to daughter cells and can be considered to be functional analogs of eucaryotic centromeres. Composite plasmids were constructed which carry either two functional P1 par regions or one F and one P1 region. Unlike dicentric chr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.158.2.742-745.1984
更新日期:1984-05-01 00:00:00
abstract::We have isolated transposon insertions in the lacA gene encoding an endogenous beta-galactosidase of Bacillus subtilis. Upstream of the putative operon containing lacA is a negative regulator, lacR, which encodes a product related to a family of regulators that includes the lactose repressor, lacI, of Escherichia coli...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.17.5636-5638.1997
更新日期:1997-09-01 00:00:00
abstract::Study of the replication pattern of a number of B. subtilis 168 strains under controlled physiological conditions revealed great interstrain variation in control of replication. Replication patterns were calculated from ratios of purA16/leu-8 and purA16/metB5 transformation frequency. The thymine-independent strains a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.123.3.1055-1067.1975
更新日期:1975-09-01 00:00:00
abstract::Two strains of Staphylococcus aureus (PC1 and 196E), when grown in medium containing 0.002% sodium dodecyl sulfate (SDS), showed from 96.1 to 100% loss of the ability to produce penicillinase. Resistance to cadmium and zinc was lost concomitantly with the ability to produce penicillinase. A comparison of the rate of c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.109.1.262-265.1972
更新日期:1972-01-01 00:00:00
abstract::Transmembrane bacterial chemoreceptors are extended, rod-shaped homodimers with ligand-binding sites at one end and interaction sites for signaling complex formation and histidine kinase control at the other. There are atomic-resolution structures of chemoreceptor fragments but not of intact, membrane-inserted recepto...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00593-17
更新日期:2018-02-07 00:00:00
abstract::Lrp (leucine-responsive regulatory protein) is a global regulator of metabolism in Escherichia coli (J. M. Calvo and R. G. Matthews, Microbiol. Rev. 58:466-490, 1994). The lrp genes from three other enteric microorganisms, Enterobacter aerogenes, Klebsiella aerogenes, and Salmonella typhimurium, were cloned and sequen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.6.1624-1626.1995
更新日期:1995-03-01 00:00:00
abstract::The DNA region encoding early nodulation functions of Bradyrhizobium japonicum 3I1b110 (I110) was isolated by its homology to the functionally similar region from Rhizobium meliloti. Isolation of a number of overlapping recombinant clones from this region allowed the construction of a restriction map of the region. Th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.164.3.1301-1308.1985
更新日期:1985-12-01 00:00:00