Physiological function of superoxide dismutase in glucose-limited chemostat cultures of Escherichia coli.

Abstract:

:Conditions for continuous culture of Escherichia coli K-12 His- Thi- under glucose limitation were established. Both the capacity for respiration, at D greater than 0.2/h, and specific activity of superoxide dismutase increased as a function of specific growth rate, whereas peroxidase and catalase were either invariant with or inversely related to this growth rate. The abrupt increase in the availability of glucose, as a means of elevating the growth rate, was followed by an increase in superoxide dismutase, which reached a plateau before there was a significant increase in the growth rate. Thus, an increase in superoxide dismutase appeared to be a prerequisite for an increase in the rate of growth. Cells that had higher levels of superoxide dismutase, because of varying specific growth rates, were more resistant to the toxicity of hyperbaric oxygen. Superoxide dismutase thus behaved like an essential defense against the toxicity of oxygen. Sensitivity towards streptonigrin increased with specific growth rate in the range of 0.09 to 0.25/h but decreased with further increases in the growth rate. Since this antibiotic has been shown to shunt electrons to oxygen, with concomitant production of O2-, these results indicated a progressive deficiency of reducing power at growth rates below 0.25/h and a surfeit of reducing power with progressively greater protection against O2- by superoxide dismutase at growth rates greater than 0.25/h.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Hassan HM,Fridovich I

doi

10.1128/JB.130.2.805-811.1977

subject

Has Abstract

pub_date

1977-05-01 00:00:00

pages

805-11

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

130

pub_type

杂志文章
  • Defect in the formation of 70S ribosomes caused by lack of ribosomal protein L34 can be suppressed by magnesium.

    abstract::To elucidate the biological functions of the ribosomal protein L34, which is encoded by the rpmH gene, the rpmH deletion mutant of Bacillus subtilis and two suppressor mutants were characterized. Although the ΔrpmH mutant exhibited a severe slow-growth phenotype, additional mutations in the yhdP or mgtE gene restored ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01896-14

    authors: Akanuma G,Kobayashi A,Suzuki S,Kawamura F,Shiwa Y,Watanabe S,Yoshikawa H,Hanai R,Ishizuka M

    更新日期:2014-11-01 00:00:00

  • Enterotoxin B synthesis by replicating and nonreplicating cells of Staphylococcus aureus.

    abstract::Although 95% of the enterotoxin B produced by Staphylococcus aureus appears during the latter part of the exponential phase of growth, growth per se is not necessary for toxin synthesis. A procedure is described whereby a concentrated suspension (at least 6 x 10(10) cells per ml) of a 16-hr culture of S. aureus was fo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.97.2.506-512.1969

    authors: Markus Z,Silverman GJ

    更新日期:1969-02-01 00:00:00

  • Expression of the Rhodobacter sphaeroides cytochrome c2 structural gene.

    abstract::A Rhodobacter sphaeroides mutant (CYCA1) lacking cytochrome c2 (cyt c2) was previously constructed (T. J. Donohue, A. G. McEwan, S. Van Doren, A. R. Crofts, and S. Kaplan, Biochemistry, 27: 1918-1924, 1988) by a combination of in vivo and in vitro molecular genetic techniques. CYCA1 was incapable of photosynthetic gro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.1.360-368.1989

    authors: Brandner JP,McEwan AG,Kaplan S,Donohue TJ

    更新日期:1989-01-01 00:00:00

  • Single-stranded DNA binding by F TraI relaxase and helicase domains is coordinately regulated.

    abstract::Transfer of conjugative plasmids requires relaxases, proteins that cleave one plasmid strand sequence specifically. The F plasmid relaxase TraI (1,756 amino acids) is also a highly processive DNA helicase. The TraI relaxase activity is located within the N-terminal approximately 300 amino acids, while helicase motifs ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00154-10

    authors: Dostál L,Schildbach JF

    更新日期:2010-07-01 00:00:00

  • Nucleotide sequence of the traI (helicase I) gene from the sex factor F.

    abstract::A 6.9-kilobase region of the Escherichia coli F plasmid containing the 3' half of the traD gene and the entire traI gene (encodes the TraI protein, DNA helicase I and TraI, a polypeptide arising from an internal in-frame translational start in traI) has been sequenced. A previously unidentified open reading frame (ten...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.7.4127-4131.1990

    authors: Bradshaw HD Jr,Traxler BA,Minkley EG Jr,Nester EW,Gordon MP

    更新日期:1990-07-01 00:00:00

  • Mutational analysis of the signal-sensing domain of ResE histidine kinase from Bacillus subtilis.

    abstract::The Bacillus subtilis ResD-ResE two-component regulatory system activates genes involved in nitrate respiration in response to oxygen limitation or nitric oxide (NO). The sensor kinase ResE activates the response regulator ResD through phosphorylation, which then binds to the regulatory region of genes involved in ana...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.186.6.1694-1704.2004

    authors: Baruah A,Lindsey B,Zhu Y,Nakano MM

    更新日期:2004-03-01 00:00:00

  • Molecular and expression analysis of the Rhizobium meliloti phosphoenolpyruvate carboxykinase (pckA) gene.

    abstract::The pckA gene of Rhizobium meliloti, encoding phosphoenolpyruvate carboxykinase, was isolated from a genomic cosmid library by complementation of the succinate growth phenotype of a Pck- mutant. The gene region was mapped by subcloning and Tn5 insertion mutagenesis. The DNA sequence for a 2-kb region containing the st...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.6.1452-1460.1995

    authors: Osterås M,Driscoll BT,Finan TM

    更新日期:1995-03-01 00:00:00

  • A global investigation of the Bacillus subtilis iron-sparing response identifies major changes in metabolism.

    abstract::The Bacillus subtilis ferric uptake regulator (Fur) protein is the major sensor of cellular iron status. When iron is limiting for growth, derepression of the Fur regulon increases the cellular capacity for iron uptake and mobilizes an iron-sparing response mediated in large part by a small noncoding RNA named FsrA. F...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05990-11

    authors: Smaldone GT,Revelles O,Gaballa A,Sauer U,Antelmann H,Helmann JD

    更新日期:2012-05-01 00:00:00

  • Reserve carbohydrate metabolism in Saccharomyces cerevisiae: responses to nutrient limitation.

    abstract::The amounts of glycogen and trehalose have been measured in cells of a prototrophic diploid yeast strain subjected to a variety of nutrient limitations. Both glycogen and trehalose were accumulated in cells deprived specifically of nirogen, sulfur, or phosphorus, suggesting that reserve carbohydrate accumulation is a ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.143.3.1384-1394.1980

    authors: Lillie SH,Pringle JR

    更新日期:1980-09-01 00:00:00

  • Isolation and characterization of a mutant of Escherichia coli blocked in the synthesis of putrescine.

    abstract::A mutant of Escherichia coli is described which is defective in the conversion of arginine to putrescine. The activity of the enzyme agmatine ureohydrolase is greatly reduced, whereas the activity of the other two enzymes of the pathway, the constitutive arginine decarboxylase and the inducible arginine decarboxylase,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.101.3.725-730.1970

    authors: Hirshfield IN,Rosenfeld HJ,Leifer Z,Maas WK

    更新日期:1970-03-01 00:00:00

  • THE GENUS VEILLONELLA. I. GENERAL CULTURAL, ECOLOGICAL, AND BIOCHEMICAL CONSIDERATIONS.

    abstract::Rogosa, M. (National Institutes of Health, Bethesda, Md.). The genus Veillonella. I. General cultural, ecological, and biochemical considerations. J. Bacteriol. 87:162-170. 1964.-Arguments are presented for excluding Veillonella discoides, V. reniformis, V. orbiculus, and V. vulvovaginitidis from the genus, and for re...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.87.1.162-170.1964

    authors: ROGOSA M

    更新日期:1964-01-01 00:00:00

  • Enzymatic formation, stability, and spontaneous reactions of 4-fluoromuconolactone, a metabolite of the bacterial degradation of 4-fluorobenzoate.

    abstract::Enzymatic conversion of 4-fluorocatechol in the simultaneous presence of partially purified preparations of catechol 1,2-dioxygenase from Pseudomonas cepacia and muconate cycloisomerase from Alcaligenes eutrophus 335 yielded a product that was unambiguously identified as (+)-4-fluoromuconolactone [(+)-4-carboxymethyl-...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.9.5119-5129.1990

    authors: Schlömann M,Fischer P,Schmidt E,Knackmuss HJ

    更新日期:1990-09-01 00:00:00

  • Distribution of glycerophospholipid-cholesterol acyltransferase in selected bacterial species.

    abstract::The distribution of glycerophospholipid-cholesterol acyltransferase in selected bacterial species was examined. Enzyme activity was demonstrated in cell-free growth media from all members of the family Vibrionaceae which were tested except Plesiomonas shigelloides. In each case, enzyme was produced in exponential to e...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.139.1.132-136.1979

    authors: MacIntyre S,Trust TJ,Buckley JT

    更新日期:1979-07-01 00:00:00

  • Escherichia coli Lrp (leucine-responsive regulatory protein) does not directly regulate expression of the leu operon promoter.

    abstract::Studies by R. Lin et al. (J. Bacteriol. 174:1948-1955, 1992) suggested that the Escherichia coli leu operon might be a member of the Lrp regulon. Their results were obtained with a leucine auxotroph; in leucine prototrophs grown in a medium lacking leucine, there was little difference in leu operon expression between ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.20.6547-6551.1999

    authors: Landgraf JR,Boxer JA,Calvo JM

    更新日期:1999-10-01 00:00:00

  • Variations in O-antigen biosynthesis and O-acetylation associated with altered phage sensitivity in Escherichia coli 4s.

    abstract::The O polysaccharide of the lipopolysaccharide (O antigen) of Gram-negative bacteria often serves as a receptor for bacteriophages that can make the phage dependent on a given O-antigen type, thus supporting the concept of the adaptive significance of the O-antigen variability in bacteria. The O-antigen layer also mod...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02398-14

    authors: Knirel YA,Prokhorov NS,Shashkov AS,Ovchinnikova OG,Zdorovenko EL,Liu B,Kostryukova ES,Larin AK,Golomidova AK,Letarov AV

    更新日期:2015-03-01 00:00:00

  • Diverse substrate range of a Flavobacterium pentachlorophenol hydroxylase and reaction stoichiometries.

    abstract::An understanding of the enzymatic reactions catalyzing the degradation of substituted phenols, a major group of environmental pollutants, is required for the development of biological methods for the decontamination of halophenol-polluted sites. We found that a flavomonooxygenase, pentachlorophenol hydroxylase, isolat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.9.2898-2902.1992

    authors: Xun L,Topp E,Orser CS

    更新日期:1992-05-01 00:00:00

  • Genomic analysis of Rickettsia japonica strain YHT.

    abstract::Rickettsia japonica strain YH, isolated in 1984 in Japan, is the type strain of R. japonica, the tick-borne agent of Japanese spotted fever. Here, we report the 1.33-Mb genome of this rickettsial species. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01928-12

    authors: Dong X,El Karkouri K,Robert C,Raoult D,Fournier PE

    更新日期:2012-12-01 00:00:00

  • TspO as a modulator of the repressor/antirepressor (PpsR/AppA) regulatory system in Rhodobacter sphaeroides 2.4.1.

    abstract::The TspO outer membrane protein of Rhodobacter sphaeroides has been shown to be involved in controlling the transcription of a number of genes which encode enzymes involved in photopigment biosynthesis and the puc operon. The display of regulated genes appears identical to those genes encompassing the PpsR/AppA repres...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.21.6355-6364.2001

    authors: Zeng X,Kaplan S

    更新日期:2001-11-01 00:00:00

  • The glycyl radical enzyme TdcE can replace pyruvate formate-lyase in glucose fermentation.

    abstract::Mutants of Escherichia coli unable to synthesize a functional pyruvate formate-lyase (PFL) are severely impaired in their capacity to grow by glucose fermentation. In a functional complementation assay designed to isolate the pfl gene from Clostridium butyricum, we fortuitously identified a gene that did not encode a ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.14.3509-3516.1998

    authors: Sawers G,Hesslinger C,Muller N,Kaiser M

    更新日期:1998-07-01 00:00:00

  • Regulation of amino acid transport in Escherichia coli by transcription termination factor rho.

    abstract::Amino acid transport rates and amino acid binding proteins were examined in a strain containing the rho-120 mutation (formerly SuA), which has been shown to lower the rho-dependent, ribonucleic acid-activated adenosine triphosphatase activity to 9% of the rho activity in the isogenic wild-type strain. Tryptophan and p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.130.3.1024-1029.1977

    authors: Quay SC,Oxender DL

    更新日期:1977-06-01 00:00:00

  • Mutations that define the promoter of ompF, a gene specifying a major outer membrane porin protein.

    abstract::Expression of the major porin structural genes, ompF and ompC, is influenced by medium osmotic strength and requires the products of two regulatory genes, ompR and envZ. To help define the sites required for the expression of both porin genes, we have used a novel selection to identify mutations that decrease ompF tra...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.162.3.1054-1060.1985

    authors: Taylor RK,Garrett S,Sodergren E,Silhavy TJ

    更新日期:1985-06-01 00:00:00

  • Ultraviolet-sensitive mutator strain of Escherichia coli K-12.

    abstract::An ultraviolet (UV)-sensitive mutator gene, mutU, was identified in Escherichia coli K-12. The mutation mutU4 is very close to uvrD, between metE and ilv, on the E. coli chromosome. It was recessive as a mutator and as a UV-sensitive mutation. The frequency of reversion of trpA46 on an F episome was increased by mutU4...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.113.1.145-160.1973

    authors: Siegel EC

    更新日期:1973-01-01 00:00:00

  • Development of a membrane-bound resiratory system prior to and during sporulation in Bacillus cereus and its relationship to membrane structure.

    abstract::Bulk membrane fragments were prepared from cells of Bacillus cereus ATCC 4342 harvested at different stages of growth and sporulation and examined for enzymes involved in electron transport functions. The presence of succinate: DCPIP oxidoreductase (EC 1.3.99.1), succinate: cytochrome c oxidoreductase (EC 1.3.2.1), NA...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.110.3.968-977.1972

    authors: Lang DR,Felix J,Lundgren DG

    更新日期:1972-06-01 00:00:00

  • Escherichia coli mutants with altered ribosomal ribonucleic acid metabolism.

    abstract::This paper reports the partial characterization of two temperature-sensitive mutants of Escherichia coli with alterations in ribosomal ribonucleic acid (rRNA) metabolism at the restrictive temperature. Both mutants continue to synthesize deoxyribonucleic acid and protein at 42 C but showed little or no accumulation of...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.125.3.1057-1073.1976

    authors: Jackson JM 3rd,Chaney SG

    更新日期:1976-03-01 00:00:00

  • Mismatch correction in pneumococcal transformation: donor length and hex-dependent marker efficiency.

    abstract::A hypothesis that preferential rejection of donor markers by the hex system of pneumococcus is due to lethal double-strand breaks has been examined in terms of its implications for the extent of the excision required. Experiments reported here were directed at asking whether hex-dependent marker efficiency depends on ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.125.1.125-135.1976

    authors: Guild WR,Shoemaker NB

    更新日期:1976-01-01 00:00:00

  • Functional complementation of the essential gene fabG1 of Mycobacterium tuberculosis by Mycobacterium smegmatis fabG but not Escherichia coli fabG.

    abstract::Mycolic acids are a key component of the mycobacterial cell wall, providing structure and forming a major permeability barrier. In Mycobacterium tuberculosis mycolic acids are synthesized by type I and type II fatty acid synthases. One of the enzymes of the type II system is encoded by fabG1. We demonstrate here that ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01740-06

    authors: Parish T,Roberts G,Laval F,Schaeffer M,Daffé M,Duncan K

    更新日期:2007-05-01 00:00:00

  • The RecF pathway of homologous recombination can mediate the initiation of DNA damage-inducible replication of the Escherichia coli chromosome.

    abstract::DNA damage-inducible DNA replication in SOS-induced Escherichia coli cells, termed inducible stable DNA replication (iSDR), has previously been shown to require either the RecBCD or the RecE pathway of homologous recombination for initiation. Here, we demonstrate that recB recC sbcC quadruple mutant cells are capable ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.22.7113-7114.1994

    authors: Asai T,Kogoma T

    更新日期:1994-11-01 00:00:00

  • Flagellar assembly in Salmonella typhimurium: analysis with temperature-sensitive mutants.

    abstract::The process of flagellar assembly in Salmonella typhimurium was investigated by using temperature-sensitive mutants. The mutants were grown at the restrictive temperature and then at the permissive temperature, with radiolabel supplied in the first phase of the experiment and not the second, or vice versa. Flagellar h...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.3.1327-1339.1990

    authors: Jones CJ,Macnab RM

    更新日期:1990-03-01 00:00:00

  • Apparent and real recombination frequencies in multicopy plasmids: the need for a novel approach in frequency determination.

    abstract::Recombination studies of bacteria are often carried out with multicopy plasmids, and recombination frequencies are often deduced from the proportion of cells in the population that express a recombinant phenotype. These frequencies should however be called apparent frequencies, since detection of the recombinant cells...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.3.754-761.1997

    authors: Chédin F,Dervyn R,Ehrlich SD,Noirot P

    更新日期:1997-02-01 00:00:00

  • Bacillus subtilis NhaC, an Na+/H+ antiporter, influences expression of the phoPR operon and production of alkaline phosphatases.

    abstract::When Bacillus subtilis is subjected to phosphate starvation, genes of the Pho regulon are either induced or repressed. Among those induced are genes encoding alkaline phosphatases (APases). A set of isogenic mutants, with a beta-galactosidase gene transcriptionally fused to the inactivated target gene, was used to ide...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.8.2505-2515.2001

    authors: Prágai Z,Eschevins C,Bron S,Harwood CR

    更新日期:2001-04-01 00:00:00