Isolation and characterization of a mutant of Escherichia coli blocked in the synthesis of putrescine.

abstract：:Strains derived from HfrH carrying the arcA2 null mutation exhibit a higher respiratory rate, enhanced glucose consumption, and a more-reduced intracellular redox state than arcA deletion mutants of a different lineage. The phenotype of the arcA2 mutants was due to the presence of a creC constitutive mutation introduc...

journal_title：Journal of bacteriology

authors： Nikel PI,de Almeida A,Pettinari MJ,Méndez BS

更新日期：2008-05-01 00:00:00

abstract：:The pckA gene of Rhizobium meliloti, encoding phosphoenolpyruvate carboxykinase, was isolated from a genomic cosmid library by complementation of the succinate growth phenotype of a Pck- mutant. The gene region was mapped by subcloning and Tn5 insertion mutagenesis. The DNA sequence for a 2-kb region containing the st...

journal_title：Journal of bacteriology

authors： Osterås M,Driscoll BT,Finan TM

更新日期：1995-03-01 00:00:00

abstract：:In contrast to the absolute Na(+) requirement for anaerobic growth of Aerobacter aerogenes on citrate as sole carbon source, aerobic growth of this microorganism did not require the presence of Na(+). However, Na(+) (optimal concentration, 10 mm) did increase the maximal amount of aerobic growth by 60%, even though it...

journal_title：Journal of bacteriology

authors： O'Brien RW,Stern JR

更新日期：1969-08-01 00:00:00

abstract：:Mycoplasmas that are known to exhibit gliding motility possess a differentiated tip structure. This polar organelle mediates cytadherence and gliding motor activity and contains a cytoskeleton-like component that provides structural support. Here, we describe gliding motility and a unique cytoskeleton in Mycoplasma in...

journal_title：Journal of bacteriology

authors： Relich RF,Friedberg AJ,Balish MF

更新日期：2009-08-01 00:00:00

abstract：:Glycerol is a primary energy source for heterotrophic haloarchaea and a major component of "salty" biodiesel waste. Glycerol is catabolized solely by glycerol kinase (encoded by glpK) to glycerol-3-phosphate (G3P) in Haloferax volcanii. Here we characterized the next critical step of this metabolic pathway: the conver...

journal_title：Journal of bacteriology

authors： Rawls KS,Martin JH,Maupin-Furlow JA

更新日期：2011-09-01 00:00:00

abstract：:In Salmonella typhimurium, three groups of genes located in rfb, rfe, and rff clusters are known to be involved in the biosynthesis of the enterobacterial common antigen. We found that enzymatic synthesis of uridine diphosphate N-acetylmannosaminouric acid, the activated form of a constituent sugar of the common antig...

journal_title：Journal of bacteriology

authors： Lew HC,Nikaido H,Mäkelä PH

更新日期：1978-10-01 00:00:00

abstract：:A new method for measuring the secretion of H(2)O(2) has been based upon an H(2)O(2)-dependent inhibition of catalase by 3-amino-1,2,4-triazole. The conversion of an H(2)O(2)-secretion rate into a catalase inhibition rate amplified a relatively small molar concentration of H(2)O(2) and provided a highly specific and s...

journal_title：Journal of bacteriology

authors： Cohen G,Somerson NL

更新日期：1969-05-01 00:00:00

abstract：:Starvation and cell density regulate the developmental expression of Myxococcus xanthus gene 4521. Three classes of mutants allow expression of this developmental gene during growth on nutrient agar, such that colonies of strains containing a Tn5 lac Omega4521 fusion are Lac(+). One class of these mutants inactivates ...

journal_title：Journal of bacteriology

authors： Guo D,Wu Y,Kaplan HB

更新日期：2000-08-01 00:00:00

abstract：:Eklund, Curtis (The University of Texas, Austin) and Orville Wyss. Enzyme associated with bacteriophage infection. J. Bacteriol. 84:1209-1215. 1962.-A capsule-digesting enzyme was formed when azotobacter cells were infected with bacteriophage. The enzyme appeared in the medium when the phages lysed the cells. By disru...

journal_title：Journal of bacteriology

authors： Eklund C,Wyss O

更新日期：1962-12-01 00:00:00

abstract：:In Escherichia coli, rotation of the flagellar motor has been shown to depend upon electrostatic interactions between charged residues of the stator protein MotA and the rotor protein FliG. These charged residues are conserved in the Na+-driven polar flagellum of Vibrio alginolyticus, but mutational studies in V. algi...

journal_title：Journal of bacteriology

authors： Yakushi T,Yang J,Fukuoka H,Homma M,Blair DF

更新日期：2006-02-01 00:00:00

abstract：:The Salmonella typhimurium pi-histidine episome was identified by Ames et al. (2) in an unstable partial revertant of a deletion mutation-containing strain, hisG203. HisG203 lacks the histidine operator, promoter, and part of the first structural gene. In this paper, we study some properties of pi factor and demonstra...

journal_title：Journal of bacteriology

authors： Levinthal M,Yeh J

更新日期：1972-03-01 00:00:00

abstract：:In Enterococcus faecalis, the site 2 protease Eep generates sex pheromones, including cAM373. Intriguingly, in Staphylococcus aureus, a peptide similar to cAM373, named cAM373_SA, is produced from the camS gene. Here, we report that the staphylococcal Eep homolog is not only responsible for the production of cAM373_SA...

journal_title：Journal of bacteriology

authors： Cheng D,Lv H,Yao Y,Cheng S,Huang Q,Wang H,Liu X,Bae T,Li M,Liu Q

更新日期：2020-07-09 00:00:00

abstract：:Clostridium difficile, a proteolytic Gram-positive anaerobe, has emerged as a significant nosocomial pathogen. Stickland fermentation reactions are thought to be important for growth of C. difficile and appear to influence toxin production. In Stickland reactions, pairs of amino acids donate and accept electrons, gene...

journal_title：Journal of bacteriology

authors： Bouillaut L,Self WT,Sonenshein AL

更新日期：2013-02-01 00:00:00

abstract：:To study the role of the cis-acting element(s) in controlling the expression of the cytochrome P450(BM-1) gene and its upstream regulatory gene, bm1P1, in Bacillus megaterium, various deletion derivatives were constructed. A 53-bp inverted repeat located midway between the P450(BM-1) gene and bm1P1 gene was found in v...

journal_title：Journal of bacteriology

authors： Shaw GC,Sung CC,Liu CH,Kao HS

更新日期：1997-01-01 00:00:00

abstract：:Three genes were shown to provide functions specific for ferric enterobactin transport in Escherichia coli: fepA encoded the outer membrane receptor, fepB produced a periplasmic protein, and the fepC product was presumably a component of a cytoplasmic membrane permease system for this siderophore. A 10.6-kilobase-pair...

journal_title：Journal of bacteriology

authors： Ozenberger BA,Nahlik MS,McIntosh MA

更新日期：1987-08-01 00:00:00

abstract：:We have identified three new Haemophilus influenzae mutations causing cells to exhibit extreme hypercompetence at all stages of growth. The mutations are in murE, which encodes the meso-diaminopimelate-adding enzyme of peptidoglycan synthesis. All are point mutations causing nonconservative amino acid substitutions, t...

journal_title：Journal of bacteriology

authors： Ma C,Redfield RJ

更新日期：2000-06-01 00:00:00

abstract：:Thiamine pyrophosphate (TPP) is synthesized de novo in Salmonella typhimurium and is a required cofactor for many enzymes in the cell. Five kinase activities have been implicated in TPP synthesis, which involves joining a 4-methyl-5-(beta-hydroxyethyl)thiazole (THZ) moiety and a 4-amino-5-hydroxymethyl-2-methylpyrimid...

journal_title：Journal of bacteriology

authors： Petersen LA,Downs DM

更新日期：1997-08-01 00:00:00

abstract：:The major structural components of the P2 contractile tail are encoded in the FETUD tail gene operon. The sequences of genes F(I) and F(II), encoding the major tail sheath and tail tube proteins, have been reported previously (L. M. Temple, S. L. Forsburg, R. Calendar, and G. E. Christie, Virology 181:353-358, 1991). ...

journal_title：Journal of bacteriology

authors： Christie GE,Temple LM,Bartlett BA,Goodwin TS

更新日期：2002-12-01 00:00:00

abstract：:The essential function of penicillin-binding protein 2 (PBP2) in methicillin-susceptible Staphylococcus aureus RN4220 was clearly established by placing the pbp2 gene under control of the inducible P(spac) promoter; the resulting bacteria were unable to grow in the absence of inducer. In contrast, the deficit in PBP2 ...

journal_title：Journal of bacteriology

authors： Pinho MG,Filipe SR,de Lencastre H,Tomasz A

更新日期：2001-11-01 00:00:00

abstract：:Renaturable regions in the DNA strands of the N group plasmid pCU1 have been visualized as stem-loop structures by electron microscopy. Four such distinct structures are described, the smallest of which is within the loop of a larger one. The region of pCU1 in which these structures occur has several restriction sites...

journal_title：Journal of bacteriology

authors： Konarska-Kozlowska M,Thatte V,Iyer VN

更新日期：1983-03-01 00:00:00

abstract：UNLABELLED:FimV is a Pseudomonas aeruginosa inner membrane protein that regulates intracellular cyclic AMP (cAMP) levels-and thus type IV pilus (T4P)-mediated twitching motility and type II secretion (T2S)-by activating the adenylate cyclase CyaB. Its cytoplasmic domain contains three predicted tetratricopeptide repeat...

journal_title：Journal of bacteriology

authors： Buensuceso RN,Nguyen Y,Zhang K,Daniel-Ivad M,Sugiman-Marangos SN,Fleetwood AD,Zhulin IB,Junop MS,Howell PL,Burrows LL

更新日期：2016-07-28 00:00:00

abstract：:A rough (R) Brucella abortus 45/20 mutant was more sensitive to the bactericidal activity of polymyxin B and lactoferricin B than was its smooth (S) counterpart but considerably more resistant than Salmonella montevideo. The outer membrane (OM) and isolated lipopolysaccharide (LPS) of S. montevideo showed a higher aff...

journal_title：Journal of bacteriology

authors： Freer E,Moreno E,Moriyón I,Pizarro-Cerdá J,Weintraub A,Gorvel JP

更新日期：1996-10-01 00:00:00

abstract：:The Salmonella enterica serovar Typhi ompS2 gene codes for a 362-amino-acid outer membrane protein that contains motifs common to the porin superfamily. It is expressed at very low levels compared to the major OmpC and OmpF porins, as observed for S. enterica serovar Typhi OmpS1, Escherichia coli OmpN, and Klebsiella ...

journal_title：Journal of bacteriology

authors： Fernández-Mora M,Puente JL,Calva E

更新日期：2004-05-01 00:00:00

abstract：:Competent cell-deoxyribonucleic acid (DNA) interactions were examined using tritium-labeled homologous or heterologous native or denatured DNAs and competent Streptococcus sanguis Wicky cells (strain WE4). The DNAs used were extracted from WE4 cells, Escherichia coli B cells, and E. coli bacteriophages T2, T4, T6, and...

journal_title：Journal of bacteriology

authors： Ranhand JM

更新日期：1980-05-01 00:00:00

abstract：:The fermentative alcohol dehydrogenase of Escherichia coli is encoded by the adhE gene, which is induced under anaerobic conditions but repressed in air. Previous work suggested that induction of adhE might depend on NADH levels. We therefore directly measured the NAD+ and NADH levels for cultures growing aerobically ...

journal_title：Journal of bacteriology

authors： Leonardo MR,Dailly Y,Clark DP

更新日期：1996-10-01 00:00:00

abstract：:The essential gene efg, which complements ammonia-dependent growth (adgA) mutations in Rhodobacter capsulatus and is located at 38.1 min on the Escherichia coli chromosome, was found to code for NH3-dependent NAD synthetase. Crude extracts from a strain which overproduces the efg gene product contained up to 400 times...

journal_title：Journal of bacteriology

authors： Willison JC,Tissot G

更新日期：1994-06-01 00:00:00

abstract：:Pfs expression is required for several metabolic pathways and limits the production of autoinducer-2, a molecule proposed to play a central role in interspecies quorum sensing. The present study reveals physiological conditions and promoter DNA elements that regulate Escherichia coli pfs transcription. Pfs transcripti...

journal_title：Journal of bacteriology

authors： Kim Y,Lew CM,Gralla JD

更新日期：2006-11-01 00:00:00

abstract：:Internal and surface structures of asci and ascospores were studied by transmission electron microscopy (TEM) and by scanning electron microscopy (SEM) to establish the character and number of ascospores within the ascus of Metschnikowia krissii. Enzyme digestion with snail gut enzymes and SEM examination suggested th...

journal_title：Journal of bacteriology

authors： Talens LT,Miller MW,Miranda M

更新日期：1973-07-01 00:00:00

abstract：:Analysis of the Escherichia coli K10 capsule gene cluster identified two regions, regions 1 and 3, conserved between different group III capsule gene clusters. Region 1 encodes homologues of KpsD, KpsM, KpsT, and KpsE proteins, and region 3 encodes homologues of the KpsC and KpsS proteins. An rfaH mutation abolished K...

journal_title：Journal of bacteriology

authors： Clarke BR,Pearce R,Roberts IS

更新日期：1999-04-01 00:00:00

abstract：:In Escherichia coli, T:G mismatches in specific contexts are corrected by a very short patch (VSP) repair system. Previous studies have shown that the product of gene vsr mediates correction of T:G to C:G in the 5'CTAGG/3'GGTCC context and in some related contexts. Amber mutations that arose in CAG sequences in gene c...

journal_title：Journal of bacteriology

authors： Lieb M,Rehmat S

更新日期：1995-02-01 00:00:00