Point mutations in a peptidoglycan biosynthesis gene cause competence induction in Haemophilus influenzae.

Abstract:

:We have identified three new Haemophilus influenzae mutations causing cells to exhibit extreme hypercompetence at all stages of growth. The mutations are in murE, which encodes the meso-diaminopimelate-adding enzyme of peptidoglycan synthesis. All are point mutations causing nonconservative amino acid substitutions, two at a poorly conserved residue (G(435)-->R and G(435)-->W) and the third at a highly conserved leucine (L(361)-->S). The mutant strains have very similar phenotypes and do not exhibit any defects in cell growth, permeability, or sensitivity to peptidoglycan antibiotics. Cells retain the normal specificity of DNA uptake for the H. influenzae uptake signal sequence. The mutations do not bypass genes known to be needed for competence induction but do dramatically increase expression of genes required for the normal pathway of DNA uptake. We conclude that the mutations do not act by increasing cell permeability but by causing induction of the normal competence pathway via a previously unsuspected signal.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Ma C,Redfield RJ

doi

10.1128/jb.182.12.3323-3330.2000

keywords:

subject

Has Abstract

pub_date

2000-06-01 00:00:00

pages

3323-30

issue

12

eissn

0021-9193

issn

1098-5530

journal_volume

182

pub_type

杂志文章
  • Differences in cold adaptation of Bacillus subtilis under anaerobic and aerobic conditions.

    abstract::Bacillus subtilis, which grows under aerobic conditions, employs fatty acid desaturase (Des) to fluidize its membrane when subjected to temperature downshift. Des requires molecular oxygen for its activity, and its expression is regulated by DesK-DesR, a two-component system. Transcription of des is induced by the tem...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00384-10

    authors: Beranová J,Mansilla MC,de Mendoza D,Elhottová D,Konopásek I

    更新日期:2010-08-01 00:00:00

  • Complete genome sequence of the veterinary pathogen Staphylococcus pseudintermedius strain HKU10-03, isolated in a case of canine pyoderma.

    abstract::Staphylococcus pseudintermedius is a member of the coagulase-positive staphylococci and is the commonest cause of canine pyoderma. We report the first genome sequence of S. pseudintermedius, which shows the presence of numerous virulence factors akin to those of the related human pathogen Staphylococcus aureus. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00023-11

    authors: Tse H,Tsoi HW,Leung SP,Urquhart IJ,Lau SK,Woo PC,Yuen KY

    更新日期:2011-04-01 00:00:00

  • Transfer among Erwinia spp. and other enterobacteria of antibiotic resistance carried on R factors.

    abstract::Antibiotic resistance carried on R factors was transferred by conjugation from Escherichia coli B/r and Shigella flexneri 1a to Erwinia spp. Tetracycline resistance (TetR) carried on R factor R100 drd-56 was transferred from E. coli B/r to strains of Erwinia amylovora, E. aroideae, E. atroseptica, E. chrysanthemi, E. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.112.1.576-584.1972

    authors: Chatterjee AK,Starr MP

    更新日期:1972-10-01 00:00:00

  • Functional characterization of SsaE, a novel chaperone protein of the type III secretion system encoded by Salmonella pathogenicity island 2.

    abstract::The type III secretion system (T3SS) encoded by Salmonella pathogenicity island 2 (SPI-2) is involved in systemic infection and intracellular replication of Salmonella enterica serovar Typhimurium. In this study, we investigated the function of SsaE, a small cytoplasmic protein encoded within the SPI-2 locus, which sh...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00863-09

    authors: Miki T,Shibagaki Y,Danbara H,Okada N

    更新日期:2009-11-01 00:00:00

  • Identification of formate dehydrogenase-specific mRNA species and nucleotide sequence of the fdhC gene of Methanobacterium formicicum.

    abstract::The overlapping fdhA and fdhB genes of Methanobacterium formicicum, which encode the alpha and beta subunits, respectively, of formate dehydrogenase, were cotranscribed as part of an approximately 4.5-kb transcript. An additional gene (fdhC) upstream of fdhA was cotranscribed with fdhA and fdhB. The deduced amino acid...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.15.4997-5004.1992

    authors: White WB,Ferry JG

    更新日期:1992-08-01 00:00:00

  • Transcriptome analysis of the progressive adaptation of Lactococcus lactis to carbon starvation.

    abstract::Adaptation of Lactococcus lactis towards progressive carbon starvation is mediated by three different types of transcriptomic responses: (i) global responses, i.e., general decreases of functions linked to bacterial growth and lack of induction of the general stress response; (ii) specific responses functionally relat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.10.3589-3592.2005

    authors: Redon E,Loubiere P,Cocaign-Bousquet M

    更新日期:2005-05-01 00:00:00

  • EVIDENCE OF LYSOGENY IN DERIVATIVES OF ESCHERICHIA COLI.

    abstract::Frampton, E. W. (The University of Texas M. D. Anderson Hospital and Tumor Institute, Houston), and B. R. Brinkley. Evidence of lysogeny in derivatives of Escherichia coli. J. Bacteriol. 90:446-452. 1965.-Exposure of cultures of several thymine-requiring derivatives of Escherichia coli strain 15 to radiation [X rays o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.90.2.446-452.1965

    authors: FRAMPTON EW,BRINKLEY BR

    更新日期:1965-08-01 00:00:00

  • Conjugational recombination in resolvase-deficient ruvC mutants of Escherichia coli K-12 depends on recG.

    abstract::ruvC mutants of Escherichia coli appear to lack an activity that resolves Holliday intermediates into recombinant products. Yet, these strains produce close to normal numbers of recombinants in genetic crosses. This recombination proficiency was found to be a function of recG. A "mini-kan" insertion in recG was introd...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.17.5414-5418.1991

    authors: Lloyd RG

    更新日期:1991-09-01 00:00:00

  • Roles of ring-hydroxylating dioxygenases in styrene and benzene catabolism in Rhodococcus jostii RHA1.

    abstract::Proteomics and targeted gene disruption were used to investigate the catabolism of benzene, styrene, biphenyl, and ethylbenzene in Rhodococcus jostii RHA1, a well-studied soil bacterium whose potent polychlorinated biphenyl (PCB)-transforming properties are partly due to the presence of the related Bph and Etb pathway...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01122-07

    authors: Patrauchan MA,Florizone C,Eapen S,Gómez-Gil L,Sethuraman B,Fukuda M,Davies J,Mohn WW,Eltis LD

    更新日期:2008-01-01 00:00:00

  • The tyrT locus of Escherichia coli B.

    abstract::The tyrT (tRNA(1TYr)) locus of Escherichia coli B differs structurally from that of K-12 strains by the absence of 2 of 3.14 terminal repeat sequences. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.8.2469-2470.1996

    authors: Timms AR,Bridges BA

    更新日期:1996-04-01 00:00:00

  • Chemotaxis toward amino acids in Escherichia coli.

    abstract::Escherichia coli cells are shown to be attracted to the l-amino acids alanine, asparagine, aspartate, cysteine, glutamate, glycine, methionine, serine, and threonine, but not to arginine, cystine, glutamine, histidine, isoleucine, leucine, lysine, phenylalanine, tryptophan, tyrosine, or valine. Bacteria grown in a pro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.112.1.315-326.1972

    authors: Mesibov R,Adler J

    更新日期:1972-10-01 00:00:00

  • Use of a phase variation-specific promoter of Myxococcus xanthus in a strategy for isolating a phase-locked mutant.

    abstract::The bacterium Myxococcus xanthus alternates between two colony types distinguished by colony morphology and pigmentation. Because the two phases are interconvertible, this phenomenon has been termed phase variation. In one phase, the colonies are bright yellow, rough, and swarming. In the alternate phase, the colonies...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.17.5341-5349.1994

    authors: Laue BE,Gill RE

    更新日期:1994-09-01 00:00:00

  • Correlation between polyploidy and auxotrophic segregation in the imperfect yeast Candida albicans.

    abstract::In order to clarify the relationship between polyploidization and the capability of phenotypic switching in the imperfect yeast Candida albicans, two types of variants were isolated as segregants from a fusant, which produced a proportion of the cell population with a higher ploidy than the rest, either in a temperatu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.11.3345-3353.1994

    authors: Suzuki T,Hitomi A,Magee PT,Sakaguchi S

    更新日期:1994-06-01 00:00:00

  • Identification of elements involved in transcriptional regulation of the Escherichia coli cad operon by external pH.

    abstract::Expression of the lysine decarboxylase gene (cadA) of Escherichia coli is induced upon external acidification. To dissect the molecular mechanisms responsible for this regulation, we analyzed a 4.2-kbp region upstream from cadA. DNA sequencing revealed two long open reading frames upstream of and on the same strand as...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.2.530-540.1992

    authors: Watson N,Dunyak DS,Rosey EL,Slonczewski JL,Olson ER

    更新日期:1992-01-01 00:00:00

  • Effect of D-amino acids on structure and synthesis of peptidoglycan in Escherichia coli.

    abstract::Growth of Escherichia coli in the presence of certain D-amino acids, such as D-methionine, results in the incorporation of the D-amino acid into macromolecular peptidoglycan and can be lethal at high concentrations. Previous studies suggested that incorporation was independent of the normal biosynthetic pathway. An en...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.17.5549-5559.1992

    authors: Caparrós M,Pisabarro AG,de Pedro MA

    更新日期:1992-09-01 00:00:00

  • Identification and characterization of CAMP cohemolysin as a potential virulence factor of Riemerella anatipestifer.

    abstract::Riemerella anatipestifer is responsible for exudative septicemia in ducks. The genetic determinant of the CAMP cohemolysin, cam, from a strain of R. anatipestifer was cloned and expressed in Escherichia coli. Chromosomal DNA from serotype 19 strain 30/90 was used to construct a gene library in pBluescript II SK(-) vec...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.7.1932-1939.2002

    authors: Crasta KC,Chua KL,Subramaniam S,Frey J,Loh H,Tan HM

    更新日期:2002-04-01 00:00:00

  • Vibrio cholerae VciB promotes iron uptake via ferrous iron transporters.

    abstract::Vibrio cholerae uses a variety of strategies for obtaining iron in its diverse environments. In this study we report the identification of a novel iron utilization protein in V. cholerae, VciB. The vciB gene and its linked gene, vciA, were isolated in a screen for V. cholerae genes that permitted growth of an Escheric...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00569-08

    authors: Mey AR,Wyckoff EE,Hoover LA,Fisher CR,Payne SM

    更新日期:2008-09-01 00:00:00

  • Expression of the Rhodobacter sphaeroides hemA and hemT genes, encoding two 5-aminolevulinic acid synthase isozymes.

    abstract::The nucleotide sequences of the Rhodobacter sphaeroides hemA and hemT genes, encoding 5-aminolevulinic acid (ALA) synthase isozymes, were determined. ALA synthase catalyzes the condensation of glycine and succinyl coenzyme A, the first and rate-limiting step in tetrapyrrole biosynthesis. The hemA and hemT structural g...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.8.2292-2303.1993

    authors: Neidle EL,Kaplan S

    更新日期:1993-04-01 00:00:00

  • Regulation of Bacillus subtilis sigmaH (spo0H) and AbrB in response to changes in external pH.

    abstract::The RNA polymerase sigma subunit, sigmaH, of Bacillus subtilis is required for the transcription of genes that are induced in late-growth cultures at high cell density, including genes that function in sporulation. The expression of sigmaH-controlled genes is repressed when nutrient broth sporulation medium (Difco spo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.21.6778-6787.1997

    authors: Cosby WM,Zuber P

    更新日期:1997-11-01 00:00:00

  • Binding of homologous polymerized deoxyribonucleic acid by Streptomyces griseus.

    abstract::Germaine, G. R. (University of Minnesota, Minneapolis), and D. L. Anderson. Binding of homologous polymerized deoxyribonucleic acid by Streptomyces griseus. J. Bacteriol. 92:662-667. 1966-An irreversible P(32) deoxyribonucleic acid (DNA) binding system is described for Streptomyces griseus S104. The 69% guanine plus c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.92.3.662-667.1966

    authors: Germaine GR,Anderson DL

    更新日期:1966-09-01 00:00:00

  • Divergence among genes encoding the elongation factor Tu of Yersinia Species.

    abstract::Elongation factor Tu (EF-Tu), encoded by tuf genes, carries aminoacyl-tRNA to the ribosome during protein synthesis. Duplicated tuf genes (tufA and tufB), which are commonly found in enterobacterial species, usually coevolve via gene conversion and are very similar to one another. However, sequence analysis of tuf gen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01067-08

    authors: Isabel S,Leblanc E,Boissinot M,Boudreau DK,Grondin M,Picard FJ,Martel EA,Parham NJ,Chain PS,Bader DE,Mulvey MR,Bryden L,Roy PH,Ouellette M,Bergeron MG

    更新日期:2008-11-01 00:00:00

  • An antimicrobial peptide is produced by extracellular processing of a protein from Propionibacterium jensenii.

    abstract::A protease-activated antimicrobial peptide (PAMP) and its inactive precursor were purified from the culture supernatant of Propionibacterium jensenii LMG 3032 and characterized at the molecular level. PAMP is a 64-amino-acid cationic peptide of 6,383 Da with physicochemical features similar to those of bacteriocins fr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.13.3649-3656.2002

    authors: Faye T,Brede DA,Langsrud T,Nes IF,Holo H

    更新日期:2002-07-01 00:00:00

  • Characterization and virulence properties of Erwinia chrysanthemi lipopolysaccharide-defective, phi EC2-resistant mutants.

    abstract::Outer membrane alterations were characterized in spontaneous mutants of the Erwinia chrysanthemi 3937jRH, which were selected for resistance to bacteriophage phi EC2. All but one of the mutants analyzed were affected in their lipopolysaccharide (LPS) structure, lacking the entire heterogeneous region of apparent high ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.9.4011-4017.1987

    authors: Schoonejans E,Expert D,Toussaint A

    更新日期:1987-09-01 00:00:00

  • DnaA protein is required for replication of the minimal replicon of the broad-host-range plasmid RK2 in Escherichia coli.

    abstract::The minimal origin of replication of the broad-host-range plasmid RK2 has two potential recognition sequences for the DnaA protein of Escherichia coli. DNA transfer by transformation into a dnaA-null mutant of E. coli showed that DnaA protein is needed for replication or maintenance of mini-RK2. We isolated and purifi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.10.4703-4709.1987

    authors: Gaylo PJ,Turjman N,Bastia D

    更新日期:1987-10-01 00:00:00

  • EFFECTS OF BICARBONATE ON GROWTH OF PASTEURELLA PESTIS. I. DIFFERENTIAL RESPONSE OF VIRULENT AND AVIRULENT CELLS.

    abstract::Surgalla, M. J. (Fort Detrick, Frederick, Md.), A. W. Andrews, and C. L. Baugh. Effects of bicarbonate on growth of Pasteurella pestis. I. Differential response of virulent and avirulent cells. J. Bacteriol. 88:269-272. 1964.-Virulent Pasteurella pestis and the avirulent mutants always present in a virulent inoculum h...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.88.2.269-272.1964

    authors: SURGALLA MJ,ANDREWS AW,BAUGH CL

    更新日期:1964-08-01 00:00:00

  • Amino acid compostion of walls from single and filamentous cells of Clostridium acidiurici.

    abstract::Gaffar, Abdul (Brigham Young University, Provo, Utah), David R. Terry, and Richard D. Sagers. Amino acid composition of walls from single and filamentous cells of Clostridium acidiurici. J. Bacteriol. 91:1618-1624. 1966.-The walls from single and filamentous cells of Clostridium acidiurici were shown to contain 11 ami...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.91.4.1618-1624.1966

    authors: Gaffar A,Terry DR,Sagers RD

    更新日期:1966-04-01 00:00:00

  • Reserve carbohydrate metabolism in Saccharomyces cerevisiae: responses to nutrient limitation.

    abstract::The amounts of glycogen and trehalose have been measured in cells of a prototrophic diploid yeast strain subjected to a variety of nutrient limitations. Both glycogen and trehalose were accumulated in cells deprived specifically of nirogen, sulfur, or phosphorus, suggesting that reserve carbohydrate accumulation is a ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.143.3.1384-1394.1980

    authors: Lillie SH,Pringle JR

    更新日期:1980-09-01 00:00:00

  • Membranes of Rhodospirillum rubrum: physicochemical properties of chromatophore fractions isolated from osmotically and mechanically disrupted cells.

    abstract::Isolation of highly purified membrane fractions from phototrophically grown Rhodospirillum rubrum was achieved by velocity and isopyknic sedimentation under carefully controlled ionic conditions. Bacteriochlorophyll-rich and succinic dehydrogenase-rich chromatophores that were essentially devoid of contamination by no...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.126.3.1326-1338.1976

    authors: Collins ML,Niederman RA

    更新日期:1976-06-01 00:00:00

  • Genome sequence of Lactococcus raffinolactis strain 4877, isolated from natural dairy starter culture.

    abstract::The nonstarter lactic acid bacterium Lactococcus raffinolactis is prevalent in a wide range of environments, such as the dairy environment, but little is known about this species. Here, we present the draft genome of Lactococcus raffinolactis strain 4877, isolated from a natural mesophilic dairy starter culture. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01697-12

    authors: Meslier V,Loux V,Renault P

    更新日期:2012-11-01 00:00:00

  • Bacteriophage P1 gene 10 encodes a trans-activating factor required for late gene expression.

    abstract::Amber mutants of bacteriophage P1 were used to identify functions involved in late regulation of the P1 lytic growth cycle. A single function has been genetically identified to be involved in activation of the phage-specific late promoter sequence Ps. In vivo, P1 gene 10 amber mutants fail to trans activate a lacZ ope...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.20.6438-6445.1991

    authors: Lehnherr H,Guidolin A,Arber W

    更新日期:1991-10-01 00:00:00