Abstract:
:Vibrio cholerae uses a variety of strategies for obtaining iron in its diverse environments. In this study we report the identification of a novel iron utilization protein in V. cholerae, VciB. The vciB gene and its linked gene, vciA, were isolated in a screen for V. cholerae genes that permitted growth of an Escherichia coli siderophore mutant in low-iron medium. The vciAB operon encodes a predicted TonB-dependent outer membrane receptor, VciA, and a putative inner membrane protein, VciB. VciB, but not VciA, was required for growth stimulation of E. coli and Shigella flexneri strains in low-iron medium. Consistent with these findings, TonB was not needed for VciB-mediated growth. No growth enhancement was seen when vciB was expressed in an E. coli or S. flexneri strain defective for the ferrous iron transporter Feo. Supplying the E. coli feo mutant with a plasmid encoding either E. coli or V. cholerae Feo, or the S. flexneri ferrous iron transport system Sit, restored VciB-mediated growth; however, no stimulation was seen when either of the ferric uptake systems V. cholerae Fbp and Haemophilus influenzae Hit was expressed. These data indicate that VciB functions by promoting iron uptake via a ferrous, but not ferric, iron transport system. VciB-dependent iron accumulation via Feo was demonstrated directly in iron transport assays using radiolabeled iron. A V. cholerae vciB mutant did not exhibit any growth defects in either in vitro or in vivo assays, possibly due to the presence of other systems with overlapping functions in this pathogen.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Mey AR,Wyckoff EE,Hoover LA,Fisher CR,Payne SMdoi
10.1128/JB.00569-08subject
Has Abstractpub_date
2008-09-01 00:00:00pages
5953-62issue
17eissn
0021-9193issn
1098-5530pii
JB.00569-08journal_volume
190pub_type
杂志文章abstract::The Bacillus subtilis SpoVE integral membrane protein is essential for the heat resistance of spores, probably because of its involvement in spore peptidoglycan synthesis. We found that an SpoVE-yellow fluorescent protein (YFP) fusion protein becomes localized to the forespore during the earliest stages of engulfment,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01482-07
更新日期:2008-01-01 00:00:00
abstract::A procedure for the isolation of Bacillus subtilis mutants that appear to be defective in septum synthesis has been described. Fourteen mutants isolated with this technique were found to be located at four distinct loci on the B. subtilis chromosome. These have been designated divA, divB, divC, and divD. The four muta...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.108.3.1366-1379.1971
更新日期:1971-12-01 00:00:00
abstract::Protocatechuate 3,4-dioxygenase is a member of a family of bacterial enzymes that cleave the aromatic rings of their substrates between two adjacent hydroxyl groups, a key reaction in microbial metabolism of varied environmental chemicals. In an appropriate genetic background, it is possible to select for Acinetobacte...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.20.6478-6487.1999
更新日期:1999-10-01 00:00:00
abstract::Transmission electron microscopy was used to examine active ribosomal ribonucleic acid (rRNA) genes in two strains of Escherichia coli: N2077, deficient in the enzyme responsible for proper cleavage of the 16S sequence from the elongating nascent rRNA transcript; and N2076, functional in ribonuclease (RNase) III activ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.132.2.718-722.1977
更新日期:1977-11-01 00:00:00
abstract::Beggs, William H. (University of Minnesota, Minneapolis), and Palmer Rogers. Galactose repression of beta-galactosidase induction in Escherichia coli. J. Bacteriol. 91:1869-1874. 1966.-Galactose repression of beta-galactosidase induction in Escherichia coli was investigated to determine whether the galactose molecule ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.5.1869-1874.1966
更新日期:1966-05-01 00:00:00
abstract::Cells of Ureaplasma urealyticum that were prepared by a ruthenium red technique demonstrated an extramembranous layer of polyanions of about 15 to 30 nm in width. Application of the concanavalin A-iron dextran strain indicated that the outer surface of this layer contained glucosyl-like residues. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.128.2.658-660.1976
更新日期:1976-11-01 00:00:00
abstract::Thiocyanate hydrolase is a newly found enzyme from Thiobacillus thioparus THI 115 that converts thiocyanate to carbonyl sulfide and ammonia (Y. Katayama, Y. Narahara, Y. Inoue, F. Amano, T. Kanagawa, and H. Kuraishi, J. Biol. Chem. 267:9170-9175, 1992). We have cloned and sequenced the scn genes that encode the three ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.10.2583-2589.1998
更新日期:1998-05-01 00:00:00
abstract::A human isolate of Salmonella enteritidis which displayed strong pellicle formation during static broth culture and mannose-sensitive hemagglutination produced fimbriae which were morphologically indistinguishable from type 1 fimbriae of members of the family Enterobacteriaceae. Fimbrin was purified to homogeneity, an...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.168.1.221-227.1986
更新日期:1986-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.1.142-147.2003
更新日期:2003-01-01 00:00:00
abstract::Glucose uptake by Bacteroides succinogenes S85 was measured under conditions that maintained anaerobiosis and osmotic stability. Uptake was inhibited by compounds which interfere with electron transport systems, maintenance of proton or metal ion gradients, or ATP synthesis. The most potent inhibitors were proton and ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.2.500-506.1987
更新日期:1987-02-01 00:00:00
abstract::Both fi(+) and fi(-) type R factors function as intact replicative units when segregated into Escherichia coli minicells. Hybridization studies demonstrate that at least 95% of the deoxyribonucleic acid (DNA) in R(+) minicells is episomal in origin. About half of the DNA can be extracted in a closed circular form and ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.108.1.300-308.1971
更新日期:1971-10-01 00:00:00
abstract::Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is responsible for worldwide outbreaks of bloody diarrhea, hemorrhagic colitis, and life-threatening hemolytic uremic syndrome. After colonizing the large intestine, EHEC forms attaching and effacing (AE) lesions on intestinal epithelial cells. These lesions cause dest...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01848-06
更新日期:2007-03-01 00:00:00
abstract::Overproduction of tryptophanyl-tRNA synthetase increased trp operon expression by reducing transcription termination at the trp attenuator. The total cellular level of charged tRNATrp was not affected by increased levels of the synthetase. We propose that excess synthetase binds charged tRNATrp and reduces the concent...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.160.2.805-807.1984
更新日期:1984-11-01 00:00:00
abstract::The fermentative hyperthermophile Pyrococcus furiosus contains an NADPH-utilizing, heterotetrameric (alphabetagammadelta), cytoplasmic hydrogenase (hydrogenase I) that catalyzes both H(2) production and the reduction of elemental sulfur to H(2)S. Herein is described the purification of a second enzyme of this type, hy...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.7.1864-1871.2000
更新日期:2000-04-01 00:00:00
abstract::In many organisms, phosphatase expression and phosphate (P) uptake are coordinately regulated by the Pho regulon. In Myxococcus xanthus P limitation initiates multicellular development, a process associated with changes in phosphatase expression. We sought here to characterize the link between P acquisition and develo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01781-07
更新日期:2008-03-01 00:00:00
abstract::The nucleotide sequence of a 2.0-kilobase DNA segment containing the Clostridium acetobutylicum glnA gene was determined. The upstream region of the glnA gene contained two putative extended promoter consensus sequences (p1 and p2), characteristic of gram-positive bacteria. A third putative extended gram-positive prom...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.1.400-408.1988
更新日期:1988-01-01 00:00:00
abstract::In Klebsiella aerogenes, arylsulfatase synthesis was repressed by inorganic sulfate, sulfite, sulfide, thiosulfate, and cysteine, but not by methionine under normal growth conditions. We isolated cysteine-requiring mutants (Cys minus), and mutants (AtsS minus, AtsR minus) in which the regulation of arylsulfatase synth...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.121.1.29-35.1975
更新日期:1975-01-01 00:00:00
abstract::We report here the identification of a new lipoprotein, NlpI, in Escherichia coli K-12. The NlpI structural gene (nlpI) is located between the genes pnp (polynucleotide phosphorylase) and deaD (RNA helicase) at 71 min on the E. coli chromosome. The nlpI gene encodes a putative polypeptide of approximately 34 kDa, and ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.14.4318-4325.1999
更新日期:1999-07-01 00:00:00
abstract::Monobromoacetate (MBA) is toxic for the 1,2-dichloroethane-degrading bacterium Xanthobacter autotrophicus GJ10 at concentrations higher than 5 mM. Mutants which are able to grow on higher concentrations of MBA were isolated and found to overexpress haloacid dehalogenase, which is encoded by the dhlB gene. In mutant GJ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.5.1348-1356.1995
更新日期:1995-03-01 00:00:00
abstract::A cell surface antigen complex from Zwittergent-solubilized Myxococcus xanthus has been purified by immunoaffinity chromatography with monoclonal antibody (MAb) 1604 and by subsequent gel filtration. We propose that the cell surface antigen (CSA) 1604 complex participates in intercellular interactions. The apparent to...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.9.4655-4666.1989
更新日期:1989-09-01 00:00:00
abstract::Disruption of gene HI0894 or HI0895 in Haemophilus influenzae Rd, homologs of Escherichia coli acrAB multidrug efflux genes, caused hypersusceptibility to erythromycin, rifampin, novobiocin, and dyes such as ethidium bromide and crystal violet and increased accumulation of radioactive erythromycin, showing that these ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.21.6855-6857.1997
更新日期:1997-11-01 00:00:00
abstract::An analysis of the turnover of the major phospholipids of Micrococcus cerificans growing or nongrowing cultures. The turnover rates of (14)C-PE and (14)C-PE were 61.5% of the total phospholipid, exhibited no significant rate of turnover in either growing or nongrowing cultures. The turnover rates of PE-(14)C and PE-(3...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.107.3.806-814.1971
更新日期:1971-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.11.6052-6058.1989
更新日期:1989-11-01 00:00:00
abstract::One step in de novo pyridoxine (vitamin B6) and pyridoxal 5'-phosphate biosynthesis was predicted to be an oxidation catalyzed by an unidentified D-erythrose-4-phosphate dehydrogenase (E4PDH). To help identify this E4PDH, we purified the Escherichia coli K-12 gapA- and gapB-encoded dehydrogenases to homogeneity and te...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.10.2804-2812.1995
更新日期:1995-05-01 00:00:00
abstract::Escherichia coli K-12 deficient in galactose-1-phosphate uridyl transferase is capable of converting significant amounts of d-[1-(14)C]galactose to (14)CO(2), whereas strains deficient in other enzymes of the Leloir pathway cannot do so. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.1.558-561.1975
更新日期:1975-10-01 00:00:00
abstract::The protein surface layers on the cell walls of mosquito-pathogenic and nonpathogenic Bacillus sphaericus strains were studied by structural, biochemical, and serological methods. The surface structure of two representative insect-pathogenic strains had the form of a delicate linear array with a repeat interval of 5 n...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.1.72-79.1987
更新日期:1987-01-01 00:00:00
abstract::The DNA-binding factor BifA (previously called VF1) binds upstream of the developmentally regulated site-specific recombinase gene xisA in the cyanobacterium Anabaena sp. strain PCC 7120. Besides binding xisA, BifA also binds the glnA, rbcL, and nifH promoter regions. DNase I footprint analysis of BifA binding to glnA...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.5.1214-1223.1994
更新日期:1994-03-01 00:00:00
abstract::PCR-based site-directed mutagenesis has been used to generate 38 alanine-substitution mutations in the C-terminal 41 amino acid residues of LuxR. This region plays a critical role in the mechanism of LuxR-dependent transcriptional activation of the Vibrio fischeri lux operon during quorum sensing. The ability of the v...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.1.387-392.2001
更新日期:2001-01-01 00:00:00
abstract::NO, a free radical gas, is the signal for Nitrosomonas europaea cells to switch between different growth modes. At an NO concentration of more than 30 ppm, biofilm formation by N. europaea was induced. NO concentrations below 5 ppm led to a reversal of the biofilm formation, and the numbers of motile and planktonic (m...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.9.2781-2788.2004
更新日期:2004-05-01 00:00:00
abstract::The plant cell wall, which consists of a highly complex array of interconnecting polysaccharides, is the most abundant source of organic carbon in the biosphere. Microorganisms that degrade the plant cell wall synthesize an extensive portfolio of hydrolytic enzymes that display highly complex molecular architectures. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01701-07
更新日期:2008-08-01 00:00:00