Bacteriophage P1 gene 10 encodes a trans-activating factor required for late gene expression.

Abstract:

:Amber mutants of bacteriophage P1 were used to identify functions involved in late regulation of the P1 lytic growth cycle. A single function has been genetically identified to be involved in activation of the phage-specific late promoter sequence Ps. In vivo, P1 gene 10 amber mutants fail to trans activate a lacZ operon fusion under the transcriptional control of promoter Ps. Several P1 segments, mapping around position 95 on the P1 chromosome, were cloned into multicopy plasmid vectors. Some of the cloned DNA segments had a deleterious effect on host cells unless they were propagated in a P1 lysogenic background. By deletion and sequence analysis, the harmful effect could be delimited to a 869-bp P1 fragment, containing a 453-bp open reading frame. This open reading frame was shown to be gene 10 by sequencing the amber mutation am10.1 and by marker rescue experiments with a number of other gene 10 amber mutants. Gene 10 codes for an 18.1-kDa protein showing an unusually high density of charged amino acid residues. No significant homology to sequences present in the EMBL/GenBank data base was found, and the protein contained none of the currently known DNA-binding motifs. An in vivo trans activation assay system, consisting of gene 10 under the transcriptional control of an inducible promoter and a gene S/lacZ fusion transcribed from Ps, was used to show that gene 10 is the only phage-encoded function required for late promoter activation.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Lehnherr H,Guidolin A,Arber W

doi

10.1128/jb.173.20.6438-6445.1991

subject

Has Abstract

pub_date

1991-10-01 00:00:00

pages

6438-45

issue

20

eissn

0021-9193

issn

1098-5530

journal_volume

173

pub_type

杂志文章
  • Nucleotide sequence and transcription of the right early region of bacteriophage PRD1.

    abstract::We have sequenced the rightmost 1,700 base pairs of bacteriophage PRD1. This region encompasses the right early region and completes the sequence of all PRD1 early functions. We have also mapped the 5' initiation site of right early transcripts in vivo and in vitro. This has allowed us to assign gene XII to an open re...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.4.1889-1898.1990

    authors: Gerendasy D,Ito J

    更新日期:1990-04-01 00:00:00

  • Phospholipids and lipopolysaccharide of Aeromonas hydrophila.

    abstract::The phospholipids and lipopolysaccharide of Aeromonas hydrophila were characterized. Phosphatidylethanolamine and phosphatidylglycerol were the major phospholipid components. The outer membrane contained more phosphatidylethanolamine and less phosphatidylglycerol than the inner membrane, and the phospholipids of the o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.161.1.463-465.1985

    authors: Howard SP,Buckley JT

    更新日期:1985-01-01 00:00:00

  • Genetic Analysis of Mutacin B-Ny266, a Lantibiotic Active against Caries Pathogens.

    abstract::Bacteriocins are ribosomally synthesized proteinaceous antibacterial peptides. They selectively interfere with the growth of other bacteria. The production and secretion of bacteriocins confer a distinct ecological advantage to the producer in competing against other bacteria that are present in the same ecological ni...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00762-19

    authors: Dufour D,Barbour A,Chan Y,Cheng M,Rahman T,Thorburn M,Stewart C,Finer Y,Gong SG,Lévesque CM

    更新日期:2020-05-27 00:00:00

  • Culture of Campylobacter jejuni with sodium deoxycholate induces virulence gene expression.

    abstract::Campylobacter jejuni, a spiral-shaped gram-negative bacterium, is a leading bacterial cause of human food-borne illness. Acute disease is associated with C. jejuni invasion of the intestinal epithelium. Further, maximal host cell invasion requires the secretion of proteins termed Campylobacter invasion antigens (Cia)....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01736-07

    authors: Malik-Kale P,Parker CT,Konkel ME

    更新日期:2008-04-01 00:00:00

  • Levels of enzymes involved in the synthesis of acetate from CO2 in Clostridium thermoautotrophicum.

    abstract::The acetogenic bacterium Clostridium thermoautotrophicum, grown on methanol, glucose, or CO2-H2, contained high levels of corrinoids, formate dehydrogenase, tetrahydrofolate enzymes, carbon monoxide dehydrogenase, and hydrogenase. Cell-free extracts catalyzed pyruvate-dependent formation of acetate from methyltetrahyd...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.151.1.507-509.1982

    authors: Clark JE,Ragsdale SW,Ljungdahl LG,Wiegel J

    更新日期:1982-07-01 00:00:00

  • Mutational analysis of transmembrane regions 3 and 4 of SecY, a central component of protein translocase.

    abstract::The SecYEG heterotrimeric membrane protein complex functions as a channel for protein translocation across the Escherichia coli cytoplasmic membrane. SecY is the central subunit of the SecYEG complex and contains 10 transmembrane segments (TM1 to TM10). Previous mutation studies suggested that TM3 and TM4 are particul...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.12.3960-3969.2004

    authors: Mori H,Shimokawa N,Satoh Y,Ito K

    更新日期:2004-06-01 00:00:00

  • Stimulation of deletions in the Escherichia coli chromosome by partially induced Mucts62 prophages.

    abstract::Deletion of bacterial DNA fragments is stimulated in induced Mucts62 lysogens. The host genes located proximally to the prophage are more frequently lost than those which are unlinked to the Mu genome. Genes located on either side of a Mu genome are deleted in the same manner. Like the other Mu-induced rearrangements,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.136.2.477-483.1978

    authors: Faelen M,Toussaint A

    更新日期:1978-11-01 00:00:00

  • The tdh and serA operons of Escherichia coli: mutational analysis of the regulatory elements of leucine-responsive genes.

    abstract::The tdh promoter of Escherichia coli is induced seven- to eightfold when cells are grown in the presence of exogenous leucine. A scheme was devised to select mutants that exhibited high constitutive expression of the tdh promoter. The mutations in these strains were shown to lie within a previously identified gene (lr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.19.5944-5953.1991

    authors: Rex JH,Aronson BD,Somerville RL

    更新日期:1991-10-01 00:00:00

  • Role of Na+ cycle in cell volume regulation of Mycoplasma gallisepticum.

    abstract::The mechanism for the extrusion of Na+ from Mycoplasma gallisepticum cells was examined. Na+ efflux from cells was studied by diluting 22Na+-loaded cells into an isoosmotic NaCl solution and measuring the residual 22Na+ in the cells. Uphill 22Na+ efflux was found to be glucose dependent and linear with time over a 60-...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.8.4410-4416.1989

    authors: Shirvan MH,Schuldiner S,Rottem S

    更新日期:1989-08-01 00:00:00

  • Acid-soluble spore proteins of Bacillus subtilis.

    abstract::Acid-soluble spore proteins (ASSPs) comprise about 5% of the total protein of mature spores of different Bacillus subtilis strains. They consist of three abundant species, alpha, beta, and gamma, four less abundant species, and several minor species, alpha, beta, and gamma make up about 18, 18 and 36%, respectively, o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.146.3.972-982.1981

    authors: Johnson WC,Tipper DJ

    更新日期:1981-06-01 00:00:00

  • Differential regulation of multiple flagellins in Vibrio cholerae.

    abstract::Vibrio cholerae, the causative agent of the human diarrheal disease cholera, is a motile bacterium with a single polar flagellum. Motility has been implicated as a virulence determinant in some animal models of cholera, but the relationship between motility and virulence has not yet been clearly defined. We have begun...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.2.303-316.1998

    authors: Klose KE,Mekalanos JJ

    更新日期:1998-01-01 00:00:00

  • Analysis of plasmid deletional instability in Bacillus subtilis.

    abstract::Using a model system, we have studied deletion formation in Bacillus subtilis. When the staphylococcal plasmids pSA2100 (7.1 kilobases) and pUB110 (4.5 kilobases) were ligated to one another at their unique XbaI sites and transformed into either rec+ or recE4 strains of B. subtilis, an intramolecular recombination eve...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.162.3.1014-1023.1985

    authors: Hahn J,Dubnau D

    更新日期:1985-06-01 00:00:00

  • Isolation from Candida albicans of a functional homolog of the Saccharomyces cerevisiae KRE1 gene, which is involved in cell wall beta-glucan synthesis.

    abstract::The KRE1 gene of Saccharomyces cerevisiae, sacKRE1, appears to be involved in the synthesis of cell wall beta-glucan. S. cerevisiae strains with mutations in the KRE1 gene produce a structurally altered cell wall (1----6)-beta-glucan, which results in resistance to K1 killer toxin. We isolated the canKRE1 gene from Ca...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.21.6859-6864.1991

    authors: Boone C,Sdicu A,Laroche M,Bussey H

    更新日期:1991-11-01 00:00:00

  • Formation of an Intramolecular Periplasmic Disulfide Bond in TcpP Protects TcpP and TcpH from Degradation in Vibrio cholerae.

    abstract:UNLABELLED:TcpP and ToxR coordinately regulate transcription of toxT, the master regulator of numerous virulence factors in Vibrio cholerae. TcpP and ToxR are membrane-localized transcription factors, each with a periplasmic domain containing two cysteines. In ToxR, these cysteines form an intramolecular disulfide bond...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00338-15

    authors: Morgan SJ,French EL,Thomson JJ,Seaborn CP,Shively CA,Krukonis ES

    更新日期:2015-11-16 00:00:00

  • Altered induction of the adaptive response to alkylation damage in Escherichia coli recF mutants.

    abstract::Escherichia coli recF mutants are hypermutable when treated with methyl methanesulfonate (G. C. Walker, Mol. Gen. Genet. 152:93-103, 1977). In this study, methylation hypermutability of recF mutant strains was examined, and it was found that recF+ is required for normal induction of the adaptive response to alkylation...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.1.99-103.1989

    authors: Volkert MR

    更新日期:1989-01-01 00:00:00

  • Method for determining whether a gene of Escherichia coli is essential: application to the polA gene.

    abstract::We have developed a general method for determining whether a gene of Escherichia coli is essential for viability. The method requires cloned DNA spanning the gene in question and a reasonably detailed genetic and physical map of the cloned segment. Using this information, one constructs a deletion of the target gene i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.158.2.636-643.1984

    authors: Joyce CM,Grindley ND

    更新日期:1984-05-01 00:00:00

  • Fractionation of Newcastle disease virus by chromatography on diethylaminoethyl cellulose.

    abstract::Wilson, Dwight E. (Rensselaer Polytechnic Institute, Troy, N.Y.). Fractionation of Newcastle disease virus by chromatography on diethylaminoethyl cellulose. J. Bacteriol. 84:295-301. 1962.-The L. Kansas and NK strains of Newcastle disease virus were chromatographed on diethylaminoethyl (DEAE) cellulose ion-exchange co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.84.2.295-301.1962

    authors: WILSON DE

    更新日期:1962-08-01 00:00:00

  • Isolation and characterization of Salmonella typhimurium glyoxylate shunt mutants.

    abstract::Growth of Salmonella typhimurium on acetate as a sole carbon source requires expression of the glyoxylate shunt; however, the genes for the glyoxylate shunt enzymes have not been previously identified in S. typhimurium. In this study, we isolated transposon insertions in the genes for the two unique enzymes of this pa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.7.3029-3034.1987

    authors: Wilson RB,Maloy SR

    更新日期:1987-07-01 00:00:00

  • Isolation of pigmentation mutants of the green filamentous photosynthetic bacterium Chloroflexus aurantiacus.

    abstract::Mutants deficient in the production of bacteriochlorophyll c (Bchl c) and one mutant lacking colored carotenoids were isolated from the filamentous gliding bacterium Chloroflexus aurantiacus. Mutagenesis was achieved by using UV radiation or N-methyl-N'-nitro-N-nitrosoguanidine. Several clones were isolated that were ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.159.1.222-227.1984

    authors: Pierson BK,Keith LM,Leovy JG

    更新日期:1984-07-01 00:00:00

  • Identification and Characterization of the Major Porin of Desulfovibrio vulgaris Hildenborough.

    abstract::Due in large part to their ability to facilitate the diffusion of a diverse range of solutes across the outer membrane (OM) of Gram-negative bacteria, the porins represent one of the most prominent and important bacterial membrane protein superfamilies. Notably, for the Gram-negative bacterium Desulfovibrio vulgaris H...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00286-17

    authors: Zeng L,Wooton E,Stahl DA,Walian PJ

    更新日期:2017-10-31 00:00:00

  • The unphosphorylated receiver domain of PhoB silences the activity of its output domain.

    abstract::PhoB is the response regulator of the Pho regulon. It is composed of two distinct domains, an N-terminal receiver domain and a C-terminal output domain that binds DNA and interacts with sigma(70) to activate transcription of the Pho regulon. Phosphorylation of the receiver domain is required for activation of the prot...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.23.6592-6597.2000

    authors: Ellison DW,McCleary WR

    更新日期:2000-12-01 00:00:00

  • Isolation of Neurospora crassa bradytrophs.

    abstract::A method was developed for the isolation of Neurospora bradytrophs. The bradytrophs (representing lesions in a number of pathways) were resistant to DL-p-fluorophenylalanine when growing in a leaky fashion but were sensitive when grown in the presence of their stimulating supplement. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.140.3.1133-1136.1979

    authors: Kinsey JA

    更新日期:1979-12-01 00:00:00

  • Chemotaxis mutants of Spirochaeta aurantia.

    abstract::Five Spirochaeta aurantia chemotaxis mutants were isolated. One mutant (the che-101 mutant) never reversed, one (the che-200 mutant) flexed predominantly, two (the che-300 and che-400-1 mutants) exhibited elevated reversal frequencies, and one (the che-400 mutant) exhibited chemotactically unstimulated behavior simila...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.1.606-611.1989

    authors: Fosnaugh K,Greenberg EP

    更新日期:1989-01-01 00:00:00

  • Yield coefficients of Thiobacillus neapolitanus in continuous culture.

    abstract::Thiobacillus neapolitanus, when grown in continuous culture with thiosulfate limiting growth, possessed an apparent maximal molar growth yield of 8.0 g (dry weight) per mole of thiosulfate. The substrate requirement for energy of maintenance was the highest yet reported, amounting to 21.8 mmoles of thiosulfate per g p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.93.3.874-878.1967

    authors: Hempfling WP,Vishniac W

    更新日期:1967-03-01 00:00:00

  • Complete genome sequence of Mycobacterium xenopi type strain RIVM700367.

    abstract::Mycobacterium xenopi is a slow-growing, thermophilic, water-related Mycobacterium species. Like other nontuberculous mycobacteria, M. xenopi more commonly infects humans with altered immune function, such as chronic obstructive pulmonary disease patients. It is considered clinically relevant in a significant proportio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00482-12

    authors: Abdallah AM,Rashid M,Adroub SA,Elabdalaoui H,Ali S,van Soolingen D,Bitter W,Pain A

    更新日期:2012-06-01 00:00:00

  • Protonmotive force and bacterial sensing.

    abstract::The role of the proton gradient and external pH in the motility and chemotaxis of Bacillus subtilis was investigated. Presence of a substantial proton gradient is not necessary for motility or chemotaxis, as long as the electrical potential is sufficient to maintain motility. Changes in the proton gradient do, however...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.141.1.26-32.1980

    authors: Miller JB,Koshland DE Jr

    更新日期:1980-01-01 00:00:00

  • Metabolism of dibutylphthalate and phthalate by Micrococcus sp. strain 12B.

    abstract::Micrococcus sp. strain 12B was isolated by enriching for growth with dibutylphthalate as the sole carbon and energy source. A pathway for the metabolism of dibutylphthalate and phthalate by micrococcus sp. strain 12B is proposed: dibutylphthalate leads to monobutylphthalate leads to phthalate leads to 3,4-dihydro-3,4-...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.151.1.48-57.1982

    authors: Eaton RW,Ribbons DW

    更新日期:1982-07-01 00:00:00

  • Mechanisms of biodegradation of metal-citrate complexes by Pseudomonas fluorescens.

    abstract::Biodegradation of metal-citrate complexes by Pseudomonas fluorescens depends on the nature of the complex formed between the metal and citric acid. Bidentate Fe(III)-, Ni-, and Zn-citrate complexes were readily biodegraded, but the tridentate Cd- and Cu-citrate, and U-citrate complexes were not. The biodegradation of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.8.1989-1993.1995

    authors: Joshi-Tope G,Francis AJ

    更新日期:1995-04-01 00:00:00

  • Deoxyribonucleic acid relatedness among species of Erwinia and between Erwinia species and other enterobacteria.

    abstract::Relatedness in species of Erwinia was assessed by determining the extent of reassociation in heterologous deoxyribonucleic acid preparations. Thermal elution chromatography on hydroxyapatite was used to separate reassociated nucleotide sequences from nonreassociated sequences and to determine the thermal stability of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.110.1.12-17.1972

    authors: Brenner DJ,Fanning GR,Steigerwalt AG

    更新日期:1972-04-01 00:00:00

  • Dentilisin activity affects the organization of the outer sheath of Treponema denticola.

    abstract::Prolyl-phenylalanine-specific serine protease (dentilisin) is a major extracellular protease produced by Treponema denticola. The gene, prtP, coding for the protease was recently cloned and sequenced (K. Ishihara, T. Miura, H. K. Kuramitsu, and K. Okuda, Infect. Immun. 64:5178-5186, 1996). In order to determine the ro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.15.3837-3844.1998

    authors: Ishihara K,Kuramitsu HK,Miura T,Okuda K

    更新日期:1998-08-01 00:00:00