Yield coefficients of Thiobacillus neapolitanus in continuous culture.

Abstract:

:Thiobacillus neapolitanus, when grown in continuous culture with thiosulfate limiting growth, possessed an apparent maximal molar growth yield of 8.0 g (dry weight) per mole of thiosulfate. The substrate requirement for energy of maintenance was the highest yet reported, amounting to 21.8 mmoles of thiosulfate per g per hr. The molar growth yield, corrected for this maintenance energy requirement, was 13.9 g (dry weight) per mole of thiosulfate. It was concluded that substrate-level phosphorylation during sulfite oxidation accounted for about 45% of the adenosine triphosphate (ATP) requirement for CO(2) assimilation and maintenance during growth on limiting thiosulfate, that three sites of energy conservation exist in the electron-transport chain terminating in oxygen, and that 7.8 moles of ATP are required to fix and assimilate 1 mole of CO(2) into cell material.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Hempfling WP,Vishniac W

doi

10.1128/JB.93.3.874-878.1967

subject

Has Abstract

pub_date

1967-03-01 00:00:00

pages

874-8

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

93

pub_type

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