Purification and partial characterization of a Nocardia brasiliensis extracellular protease.

abstract：:Ribonucleotide reductases (RNRs) are crucial to all living cells, since they provide deoxyribonucleotides (dNTPs) for DNA synthesis and repair. In Mycobacterium tuberculosis, a class Ib RNR comprising nrdE- and nrdF2-encoded subunits is essential for growth in vitro. Interestingly, the genome of this obligate human pa...

journal_title：Journal of bacteriology

authors： Mowa MB,Warner DF,Kaplan G,Kana BD,Mizrahi V

更新日期：2009-02-01 00:00:00

abstract：:Genetic circularity of the Pseudomonas aeruginosa PAO chromosome was demonstrated by a series of two- and three-factor crosses and double-selection experiments with Cma plasmids FP2, FP5, FP110, and R68.45. A range of additional markers, including catabolic markers, were located on the chromosome map. Plasmid FP2, kno...

journal_title：Journal of bacteriology

authors： Royle PL,Matsumoto H,Holloway BW

更新日期：1981-01-01 00:00:00

abstract：:The genetic context, phylogeny, and biochemistry of a gene flanking the H(2)-forming methylene-H(4)-methanopterin dehydrogenase gene (hmdA), here designated hmdB, indicate that it is a new member of the radical S-adenosylmethionine enzyme superfamily. In contrast to the characteristic CX(3)CX(2)C or CX(2)CX(4)C motif ...

journal_title：Journal of bacteriology

authors： McGlynn SE,Boyd ES,Shepard EM,Lange RK,Gerlach R,Broderick JB,Peters JW

更新日期：2010-01-01 00:00:00

abstract：:The cis-acting regulatory regions of the tps and ops genes of Myxococcus xanthus were localized by analyzing the expression of fusions of these genes with the lacZ gene. A 201-base-pair (bp) fragment of tps DNa extending 95 bp upstream (-95) from the transcriptional start was sufficient to direct developmentally regul...

journal_title：Journal of bacteriology

authors： Downard JS,Kim SH,Kil KS

更新日期：1988-10-01 00:00:00

abstract：:Exogenous proline specifically stimulates the growth rate of enteric bacteria in media of inhibitory osmotic strength (J. H. B. Christian, Aust. J. Biol. Sci. 8:490-497, 1955). I observed that Salmonella typhimurium mutants which lack both of the previously known proline permeases (putP proP) are stimulated by proline...

journal_title：Journal of bacteriology

authors： Csonka LN

更新日期：1982-09-01 00:00:00

abstract：:In Escherichia coli, the additon of ethanol resulted in the synthesis of an increased proportion of phospholipids containing two unsaturated fatty acids. The addition of hexanol resulted in the opposite effect, an increase in the proportion of monounsaturated molecular species. The alcohol-induced changes were quantit...

journal_title：Journal of bacteriology

authors： Berger B,Carty CE,Ingram LO

更新日期：1980-06-01 00:00:00

abstract：:Pelosinus fermentans 16S rRNA gene sequences have been reported from diverse geographical sites since the recent isolation of the type strain. We present the genome sequence of the P. fermentans type strain R7 (DSM 17108) and genome sequences for two new strains with different abilities to reduce iron, chromate, and u...

journal_title：Journal of bacteriology

authors： Brown SD,Podar M,Klingeman DM,Johnson CM,Yang ZK,Utturkar SM,Land ML,Mosher JJ,Hurt RA Jr,Phelps TJ,Palumbo AV,Arkin AP,Hazen TC,Elias DA

更新日期：2012-09-01 00:00:00

abstract：:The ethanol oxidase respiratory chain of Gluconobacter suboxydan was characterized by using G. suboxydans subsp. alpha, a variant species of G. suboxydans incapable of oxidizing ethanol. The membranes of G. suboxydans subsp. alpha exhibited neither alcohol dehydrogenase, ethanol oxidase, nor glucose-ferricyanide oxido...

journal_title：Journal of bacteriology

authors： Matsushita K,Nagatani Y,Shinagawa E,Adachi O,Ameyama M

更新日期：1991-06-01 00:00:00

abstract：:Mutants of Agrobacterium tumefaciens requiring methionine for growth on a solid basal medium were induced by the use of N-methyl-N'-nitro-N-nitrosoguanidine. In addition to the difference of mutant strains, the extent of methionine dependency differed in a liquid basal medium and in the presence of aspartate or fumara...

journal_title：Journal of bacteriology

authors： Kaneshiro T

更新日期：1968-06-01 00:00:00

abstract：:Bacteria have evolved numerous means of survival in adverse environments with dormancy, as represented by "persistence" and the "viable but nonculturable" (VBNC) state, now recognized to be common modes for such survival. VBNC cells have been defined as cells which, induced by some stress, become nonculturable on medi...

journal_title：Journal of bacteriology

authors： Ayrapetyan M,Williams T,Oliver JD

更新日期：2018-09-24 00:00:00

abstract：:In the Firmicutes, two-component regulatory systems of the LiaSR type sense and orchestrate the response to various agents that perturb cell envelope functions, in particular lipid II cycle inhibitors. In the current study, we found that the corresponding system in Streptococcus pneumoniae displays similar properties ...

journal_title：Journal of bacteriology

authors： Eldholm V,Gutt B,Johnsborg O,Brückner R,Maurer P,Hakenbeck R,Mascher T,Håvarstein LS

更新日期：2010-04-01 00:00:00

abstract：:The products of phospholipid turnover in Bacillus stearothermophilus were determined in cultures labeled to equilibrium and with short pulses of [32P]phosphate and [2-3H]glycerol. Label lost from the cellular lipid pool was recovered in three fractions: low-molecular-weight extracellular products, extracellular lipid,...

journal_title：Journal of bacteriology

authors： Card GL,Finn DJ

更新日期：1983-04-01 00:00:00

abstract：:Washed cell suspensions of Aquaspirillum magnetotacticum MS-1, A. itersonii E12639, Bacillus subtilis 6633, and Escherichia coli CSH27 translocated protons in response to the added oxidant O2 or NO3-, with triphenylmethylphosphonium bromide as the permeant ion. Iron respiration-driven proton translocation was observed...

journal_title：Journal of bacteriology

authors： Short KA,Blakemore RP

更新日期：1986-08-01 00:00:00

abstract：:The three-dimensional structure of the nickel-containing hydrogenase from Thiocapsa roseopersicina has been determined at a resolution of 2 nm in the plane and 4 nm in the vertical direction by electron microscopy and computerized image processing on microcrystals of the enzyme. The enzyme forms a large ring-shaped co...

journal_title：Journal of bacteriology

authors： Sherman MB,Orlova EV,Smirnova EA,Hovmöller S,Zorin NA

更新日期：1991-04-01 00:00:00

abstract：:Recombination studies of bacteria are often carried out with multicopy plasmids, and recombination frequencies are often deduced from the proportion of cells in the population that express a recombinant phenotype. These frequencies should however be called apparent frequencies, since detection of the recombinant cells...

journal_title：Journal of bacteriology

authors： Chédin F,Dervyn R,Ehrlich SD,Noirot P

更新日期：1997-02-01 00:00:00

abstract：:Inducible cat and erm genes are regulated by translational attenuation. In this regulatory model, gene activation results from chloramphenicol- or erythromycin-dependent stalling of a ribosome at a precise site in the leader region of cat or erm transcripts. The stalled ribosome is believed to destabilize a downstream...

journal_title：Journal of bacteriology

authors： Rogers EJ,Ambulos NP Jr,Lovett PS

更新日期：1990-11-01 00:00:00

abstract：:Members of the family Halobacteriaceae in the domain Archaea are obligate extreme halophiles. They occupy a variety of hypersaline environments, and their cellular biochemistry functions in a nearly saturated salty milieu. Despite extensive study, a detailed analysis of their growth kinetics is missing. To remedy this...

journal_title：Journal of bacteriology

authors： Robinson JL,Pyzyna B,Atrasz RG,Henderson CA,Morrill KL,Burd AM,Desoucy E,Fogleman RE 3rd,Naylor JB,Steele SM,Elliott DR,Leyva KJ,Shand RF

更新日期：2005-02-01 00:00:00

abstract：:Helicobacter pylori is a microaerophilic bacterium associated with gastric inflammation and peptic ulcers. Knowledge of how pathogenic organisms produce energy is important from a therapeutic point of view. We found d-amino acid dehydrogenase-mediated electron transport from d-proline or d-alanine to oxygen via the re...

journal_title：Journal of bacteriology

authors： Tanigawa M,Shinohara T,Nishimura K,Nagata K,Ishizuka M,Nagata Y

更新日期：2010-03-01 00:00:00

abstract：:Southern blot analysis indicated that the gene encoding the constitutive, NADP-linked bile acid 7 alpha-hydroxysteroid dehydrogenase of Eubacterium sp. strain VPI 12708 was located on a 6.5-kb EcoRI fragment of the chromosomal DNA. This fragment was cloned into bacteriophage lambda gt11, and a 2.9-kb piece of this ins...

journal_title：Journal of bacteriology

authors： Baron SF,Franklund CV,Hylemon PB

更新日期：1991-08-01 00:00:00

abstract：:One of the pleiotropic phenotypes of mutations affecting DNA gyrase activity in Salmonella typhimurium is the constitutive deattenuation of the histidine operon. In the present work, we isolated and characterized a suppressor mutation which restores his attenuation in the presence of a defective gyrase. Such a suppres...

journal_title：Journal of bacteriology

authors： Blanc-Potard AB,Bossi L

更新日期：1994-04-01 00:00:00

abstract：:Resting cells of Corynebacterium nephridii reduce nitrate, nitrite, and nitric oxide to nitrous oxide under anaerobic conditions. Nitrous oxide production from nitrite was optimal from pH 7.0 to 7.4. The stoichiometry of nitrous oxide production from nitrite was 99% of the theoretical-two moles of nitrite was used for...

journal_title：Journal of bacteriology

authors： Renner ED,Becker GE

更新日期：1970-03-01 00:00:00

abstract：:Thiocyanate hydrolase is a newly found enzyme from Thiobacillus thioparus THI 115 that converts thiocyanate to carbonyl sulfide and ammonia (Y. Katayama, Y. Narahara, Y. Inoue, F. Amano, T. Kanagawa, and H. Kuraishi, J. Biol. Chem. 267:9170-9175, 1992). We have cloned and sequenced the scn genes that encode the three ...

journal_title：Journal of bacteriology

authors： Katayama Y,Matsushita Y,Kaneko M,Kondo M,Mizuno T,Nyunoya H

更新日期：1998-05-01 00:00:00

abstract：:CatR, a LysR family protein, positively regulates the Pseudomonas putida catBC operon, which is required for growth on benzoate as a sole carbon source. Transcriptional studies show that the catR and catBC promoters are divergent and overlapping by 2 bp. A beta-galactosidase promoter probe vector was constructed to an...

journal_title：Journal of bacteriology

authors： Rothmel RK,Shinabarger DL,Parsek MR,Aldrich TL,Chakrabarty AM

更新日期：1991-08-01 00:00:00

abstract：:A reaction center H- strain (RCH-) of Rhodobacter sphaeroides, PUHA1, was made by in vitro deletion of an XhoI restriction endonuclease fragment from the puhA gene coupled with insertion of a kanamycin resistance gene cartridge. The resulting construct was delivered to R. sphaeroides wild-type 2.4.1, with the defectiv...

journal_title：Journal of bacteriology

authors： Sockett RE,Donohue TJ,Varga AR,Kaplan S

更新日期：1989-01-01 00:00:00

abstract：:The nucleotide sequence of a 2220-base-pair fragment containing the btuB gene of Escherichia coli was determined. There was a single open reading frame which was translated into a 614-amino-acid polypeptide, the first 20 amino acids of which comprised a typical leader sequence. The putative mature or processed form ha...

journal_title：Journal of bacteriology

authors： Heller K,Kadner RJ

更新日期：1985-03-01 00:00:00

abstract：:Methicillin resistance in staphylococci is mediated by PBP2a, a penicillin binding protein with low affinity for beta-lactam antibiotics. The gene encoding PBP2a, mecA, is transcriptionally regulated in some clinical isolates by mecR1 and mecI, genes divergently transcribed from mecA that encode a signal transducer an...

journal_title：Journal of bacteriology

authors： Sharma VK,Hackbarth CJ,Dickinson TM,Archer GL

更新日期：1998-04-01 00:00:00

abstract：:Krebs cycle enzyme activity in Bacillus subtilis was examined under aerobic and anaerobic conditions. Citrate synthase and aconitase activities in cells grown anaerobically in the presence of nitrate were reduced by as much as 10- and 30-fold, respectively, from levels observed under aerobic culture conditions. The ma...

journal_title：Journal of bacteriology

authors： Nakano MM,Zuber P,Sonenshein AL

更新日期：1998-07-01 00:00:00

abstract：:The psbAII gene of the cyanobacterium Synechococcus sp. strain PCC 7942 is a member of a three-gene family that encodes the D1 protein of the photosystem II reaction center. Transcription of psbAII is rapidly induced when the light intensity reaching the culture increases from 125 microE.m-2.s-1 (low light) to 750 mic...

journal_title：Journal of bacteriology

authors： Li R,Dickerson NS,Mueller UW,Golden SS

更新日期：1995-02-01 00:00:00

abstract：:Cells of Pseudomonas aeruginosa resistant to benzalkonium chloride (BC) underwent unique ultrastructural reorganizations when they were grown in the presence of 1 mg of BC/ml. The resistant cells usually contained a single, centrally positioned pseudovacuole. The pseudovacuole was surrounded by a diffuse substance tha...

journal_title：Journal of bacteriology

authors： Hoffmann HP,Geftic SG,Gelzer J,Heymann H,Adair FW

更新日期：1973-01-01 00:00:00

abstract：:The citrate utilization (Cit+) transposon Tn3411 was shown to be flanked by directly repeated sequences (IS3411L and IS3411R) by restriction enzyme analysis and electron microscope observation. Cit- deletion mutants were frequently found to be generated in pBR322::Tn3411 by intramolecular recombination between the two...

journal_title：Journal of bacteriology

authors： Ishiguro N,Sato G

更新日期：1984-11-01 00:00:00