A third L-proline permease in Salmonella typhimurium which functions in media of elevated osmotic strength.

Abstract:

:Exogenous proline specifically stimulates the growth rate of enteric bacteria in media of inhibitory osmotic strength (J. H. B. Christian, Aust. J. Biol. Sci. 8:490-497, 1955). I observed that Salmonella typhimurium mutants which lack both of the previously known proline permeases (putP proP) are stimulated by proline in media of inhibitory osmolarity. I propose that there is a third proline permease which functions only in media of elevated osmolarity. This conclusion is based on the observations that, in media of elevated osmolarity, (i) the sensitivity of putP proP mutants to toxic proline analogs increases, (ii) proline requirements for maximal growth of proline auxotrophic putP proP mutants decreases, and (iii) the specific rate of incorporation of radioactive proline into protein of growing cells increases. I obtained a Tn10-induced mutation in a gene (proU) required for the functioning of the third proline permease and determined the map location to be at 59 map units of the chromosome, between srlA and tct, 66% linked to nalB in P22 transduction. My results suggest that the function of the third, osmotically stimulated permease might be to accumulate high intracellular proline levels during osmotic stress. Possible mechanisms by which proline might cause growth stimulation are discussed.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Csonka LN

doi

10.1128/JB.151.3.1433-1443.1982

subject

Has Abstract

pub_date

1982-09-01 00:00:00

pages

1433-43

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

151

pub_type

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