Specific binding of Synechococcus sp. strain PCC 7942 proteins to the enhancer element of psbAII required for high-light-induced expression.

Abstract:

:The psbAII gene of the cyanobacterium Synechococcus sp. strain PCC 7942 is a member of a three-gene family that encodes the D1 protein of the photosystem II reaction center. Transcription of psbAII is rapidly induced when the light intensity reaching the culture increases from 125 microE.m-2.s-1 (low light) to 750 microE.m-2.s-1 (high light). The DNA segment upstream of psbAII that corresponds to the untranslated leader of its major transcript has enhancer activity and confers high-light induction. We show that one or more soluble proteins from PCC 7942 specifically bind to this region of psbAII (designated the enhancer element). In vivo footprinting showed protein binding to the enhancer element in high-light-exposed cell samples but not in those maintained at low light, even though in vitro mobility shifts were detectable with extracts from low- or high-light-grown cells. When 12 bp were deleted from the psbAII enhancer element, protein binding was impaired and high-light induction of both transcriptional and translational psbAII-lacZ reporters was significantly reduced. This finding indicates that protein binding to this region is required for high-light induction of psbAII. The mutant element also showed impaired enhancer activity when combined with a heterologous promoter.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Li R,Dickerson NS,Mueller UW,Golden SS

doi

10.1128/jb.177.3.508-516.1995

subject

Has Abstract

pub_date

1995-02-01 00:00:00

pages

508-16

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

177

pub_type

杂志文章
  • Role of a ferredoxin gene cotranscribed with the nifHDK operon in N(2) fixation and nitrogenase "switch-off" of Azoarcus sp. strain BH72.

    abstract::The endophytic diazotroph Azoarcus sp. strain BH72 is capable of infecting rice roots and of expressing the nitrogenase (nif) genes there. In order to study the genetic background for nitrogen fixation in strain BH72, the structural genes of nitrogenase (nifHDK) were cloned and sequenced. The sequence analysis reveale...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.12.3752-3760.2001

    authors: Egener T,Martin DE,Sarkar A,Reinhold-Hurek B

    更新日期:2001-06-01 00:00:00

  • Cloning and characterization of the beta-amylase gene from Bacillus polymyxa.

    abstract::The gene for beta-amylase was isolated from Bacillus polymyxa by molecular cloning in B. subtilis. B. subtilis cells containing this gene express and secrete an amylase which resembles the B. polymyxa beta-amylase and barley beta-amylase in terms of the products it generates during carbohydrate hydrolysis. Starch hydr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.165.3.819-824.1986

    authors: Friedberg F,Rhodes C

    更新日期:1986-03-01 00:00:00

  • Molecular characterization of the Salmonella enterica serovar Typhi Vi-typing bacteriophage E1.

    abstract::Some bacteriophages target potentially pathogenic bacteria by exploiting surface-associated virulence factors as receptors. For example, phage have been identified that exhibit specificity for Vi capsule producing Salmonella enterica serovar Typhi. Here we have characterized the Vi-associated E1-typing bacteriophage u...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01654-07

    authors: Pickard D,Thomson NR,Baker S,Wain J,Pardo M,Goulding D,Hamlin N,Choudhary J,Threfall J,Dougan G

    更新日期:2008-04-01 00:00:00

  • The Vibrio harveyi GTPase CgtAV is essential and is associated with the 50S ribosomal subunit.

    abstract::It was previously reported that unlike the other obg/cgtA GTPases, the Vibrio harveyi cgtAV is not essential. Here we show that cgtAV was not disrupted in these studies and is, in fact, essential for viability. Depletion of CgtAV did not result in cell elongation. CgtAV is associated with the large ribosomal particle....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.3.1205-1210.2006

    authors: Sikora AE,Zielke R,Datta K,Maddock JR

    更新日期:2006-02-01 00:00:00

  • Effects of salts on the lethality of paraquat.

    abstract::Escherichia coli suffered 95 to 100% lethality when exposed to 1.0 mM paraquat for 30 min at 37 degrees C in aerobic nutrient broth medium but did not lose viability when the exposure was done in Vogel Bonner or tryptic soy yeast extract medium. Paraquat was, however, bacteriostatic in all of these media. Salts, added...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.167.1.346-349.1986

    authors: Kitzler J,Fridovich I

    更新日期:1986-07-01 00:00:00

  • Identification and characterization of noncoding small RNAs in Streptococcus pneumoniae serotype 2 strain D39.

    abstract::We report a search for small RNAs (sRNAs) in the low-GC, gram-positive human pathogen Streptococcus pneumoniae. Based on bioinformatic analyses by Livny et al. (J. Livny, A. Brencic, S. Lory, and M. K. Waldor, Nucleic Acids Res. 34:3484-3493, 2006), we tested 40 candidates by Northern blotting and confirmed the expres...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01204-09

    authors: Tsui HC,Mukherjee D,Ray VA,Sham LT,Feig AL,Winkler ME

    更新日期:2010-01-01 00:00:00

  • Functional analysis of luxS in the probiotic strain Lactobacillus rhamnosus GG reveals a central metabolic role important for growth and biofilm formation.

    abstract::Quorum sensing is involved in the regulation of multicellular behavior through communication via small molecules. Given the high number and diversity of the gastrointestinal microbiota, it is postulated that members of this community communicate to coordinate a variety of adaptive processes. AI-2 is suggested to be a ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01394-06

    authors: Lebeer S,De Keersmaecker SC,Verhoeven TL,Fadda AA,Marchal K,Vanderleyden J

    更新日期:2007-02-01 00:00:00

  • Isolation of Neurospora crassa bradytrophs.

    abstract::A method was developed for the isolation of Neurospora bradytrophs. The bradytrophs (representing lesions in a number of pathways) were resistant to DL-p-fluorophenylalanine when growing in a leaky fashion but were sensitive when grown in the presence of their stimulating supplement. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.140.3.1133-1136.1979

    authors: Kinsey JA

    更新日期:1979-12-01 00:00:00

  • Molecular cloning, expression, and characterization of a Ca2+-dependent, membrane-associated nuclease of Mycoplasma genitalium.

    abstract::In this study, we identified and characterized the enzymatic properties of MG_186, a calcium-dependent Mycoplasma genitalium nuclease. MG_186 displays the hallmarks of nucleases, as indicated by its amino acid sequence similarity to other nucleases. We cloned, UGA corrected, expressed, purified, and demonstrated that ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00401-10

    authors: Li L,Krishnan M,Baseman JB,Kannan TR

    更新日期:2010-10-01 00:00:00

  • bop gene cluster expression in bacteriorhodopsin-overproducing mutants of Halobacterium halobium.

    abstract::mRNA levels from the bop (bacterio-opsin), brp (bacterio-opsin-related protein), and bat (bacterio-opsin activator) genes in wild-type Halobacterium halobium and two bacteriorhodopsin-overproducing mutants (ET1001 and II-7) were quantitated under conditions in which oxygen levels were steadily depleted and then cultur...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.6.1655-1660.1994

    authors: Shand RF,Betlach MC

    更新日期:1994-03-01 00:00:00

  • Role of a sugar-lipid intermediate in colanic acid synthesis by Escherichia coli.

    abstract::Membrane fractions from a lon strain of Escherichia coli but not a wild-type strain catalyze the incorporation of fucose from guanosine 5'-diphosphate-fucose into a lipid and into polymeric material. Both incorporation reactions specifically require only uridine 5'-diphosphate (UDP)-glucose. The sugar lipid was shown ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.129.1.225-236.1977

    authors: Johnson JG,Wilson DB

    更新日期:1977-01-01 00:00:00

  • Characterization of the regulatory region of a cell interaction-dependent gene in Myxococcus xanthus.

    abstract::omega 4403 is the site of a Tn5 lac insertion in the Myxococcus xanthus genome that fuses lacZ expression to a developmentally regulated promoter. Cell-cell interactions that occur during development, including C-signaling, are required for expression of Tn5 lac omega 4403. We have cloned DNA upstream of the omega 440...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.9.2539-2550.1996

    authors: Fisseha M,Gloudemans M,Gill RE,Kroos L

    更新日期:1996-05-01 00:00:00

  • Truncated forms of PspA that are secreted from Streptococcus pneumoniae and their use in functional studies and cloning of the pspA gene.

    abstract::Insertion-duplication mutagenesis was used to generate mutants of Streptococcus pneumoniae that produced truncated forms of PspA (pneumococcal surface protein A). The truncated products, representing from 20 to 80% of the complete PspA molecule, were all secreted from the cell and could be detected in unconcentrated c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.2.610-618.1992

    authors: Yother J,Handsome GL,Briles DE

    更新日期:1992-01-01 00:00:00

  • Escherichia coli mutants with an altered sensitivity to cecropin D.

    abstract::Cecropins are a family of small, basic antibacterial polypeptides which can be isolated from pupae of immunized Lepidoptera. They are active against both gram-negative and gram-positive bacteria. We studied a mutant of Escherichia coli, strain SB1004, which is more sensitive to cecropin D than is the parental strain. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.154.1.170-176.1983

    authors: Sidén I,Boman HG

    更新日期:1983-04-01 00:00:00

  • Modulation of luminescence operon expression by N-octanoyl-L-homoserine lactone in ainS mutants of Vibrio fischeri.

    abstract::Population density-dependent expression of luminescence in Vibrio fischeri is controlled by the autoinducer N-3-oxohexanoyl-L-homoserine lactone (autoinducer 1 [AI-1]), which via LuxR activates transcription of the lux operon (luxICDABEG, encoding the putative autoinducer synthase [LuxI] and the luminescence enzymes)....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.4.971-976.1996

    authors: Kuo A,Callahan SM,Dunlap PV

    更新日期:1996-02-01 00:00:00

  • SOME CYTOLOGICAL AND PATHOGENIC PROPERTIES OF SPHEROPLASTS OF CANDIDA ALBICANS.

    abstract::Kobayashi, George S. (Tulane University, New Orleans, La.), Lorraine Friedman, and Judith F. Kofroth. Some cytological and pathogenic properties of spheroplasts of Candida albicans. J. Bacteriol. 88:795-801. 1964.-Spheroplasts of Candida albicans were prepared by use of an enzymatic mixture from the digestive tract of...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.88.3.795-801.1964

    authors: KOBAYASHI GS,FRIEDMAN L,KOFROTH JF

    更新日期:1964-09-01 00:00:00

  • Identification of Agrobacterium tumefaciens genes that direct the complete catabolism of octopine.

    abstract::Agrobacterium tumefaciens R10 was mutagenized by using the promoter probe transposon Tn5-gusA7, and a library of approximately 5,000 transcriptional fusions was screened for octopine-inducible patterns of gene expression. Twenty-one mutants carrying strongly inducible gusA fusions, 20 of which showed defects in the ca...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.7.1872-1880.1996

    authors: Cho K,Fuqua C,Martin BS,Winans SC

    更新日期:1996-04-01 00:00:00

  • Phage phi29 proteins p1 and p17 are required for efficient binding of architectural protein p6 to viral DNA in vivo.

    abstract::Bacteriophage phi29 protein p6 is a viral architectural protein, which binds along the whole linear phi29 DNA in vivo and is involved in initiation of DNA replication and transcription control. Protein p1 is a membrane-associated viral protein, proposed to attach the viral genome to the cell membrane. Protein p17 is i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.24.8401-8406.2004

    authors: González-Huici V,Alcorlo M,Salas M,Hermoso JM

    更新日期:2004-12-01 00:00:00

  • nfi, the gene for endonuclease V in Escherichia coli K-12.

    abstract::Endonuclease V is specific for single-stranded DNA or for duplex DNA that contains uracil or that is damaged by a variety of agents (B. Demple and S. Linn, J. Biol. Chem. 257:2848-2855, 1982). Thus, it may be a versatile DNA repair enzyme. The protein was purified to apparent homogeneity, and from its N-terminal seque...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.2.310-316.1997

    authors: Guo G,Ding Y,Weiss B

    更新日期:1997-01-01 00:00:00

  • Growth pattern of Rickettsia tsutsugamushi in irradiated L cells.

    abstract::Irradiated L cells infected with Rickettsia tsutsugamushi were studied under the electron microscope to define the morphological growth pattern of the organism. For 2 days after inoculation, no rickettsiae were found either extra- or intracellularly; after 2 days multiple rickettsiae appeared within the host cells wit...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.154.2.879-892.1983

    authors: Hase T

    更新日期:1983-05-01 00:00:00

  • Mutations in GltC that increase Bacillus subtilis gltA expression.

    abstract::Mutants with altered forms of GltC, a positive LysR-type regulator of Bacillus subtilis glutamate synthase gene expression, were isolated. The mutant GltC proteins stimulated expression from the wild-type gltA promoter region 1.5- to 2.0-fold and from mutant promoter regions up to 80-fold. Moreover, expression of gltA...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.19.5696-5700.1995

    authors: Belitsky BR,Sonenshein AL

    更新日期:1995-10-01 00:00:00

  • Replication control mutations of plasmid R6-5 and their effects on interactions of the RNA-I control element with its target.

    abstract::Nine high copy number mutations of plasmid R6-5, representing five phenotypically distinct groups, have been identified by DNA sequencing. In each mutant plasmid examined, a single nucleotide change was found. The effects of the mutations on possible gene products, and DNA-RNA secondary structure, were analyzed and co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.154.1.429-436.1983

    authors: Brady G,Frey J,Danbara H,Timmis KN

    更新日期:1983-04-01 00:00:00

  • Identification and characterization of a membrane permease involved in iron-hydroxamate transport in Staphylococcus aureus.

    abstract::Staphylococcus aureus was shown to transport iron complexed to a variety of hydroxamate type siderophores, including ferrichrome, aerobactin, and desferrioxamine. An S. aureus mutant defective in the ability to transport ferric hydroxamate complexes was isolated from a Tn917-LTV1 transposon insertion library after sel...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.16.4394-4400.2000

    authors: Sebulsky MT,Hohnstein D,Hunter MD,Heinrichs DE

    更新日期:2000-08-01 00:00:00

  • Importance of Individual Germination Receptor Subunits in the Cooperative Function between GerA and Ynd.

    abstract::Germination of Bacillus spores is triggered by the binding of specific nutrients to germinant receptors (GRs) located in the spore's inner membrane. The GRs typically consist of A, B, and C subunits, encoded by tricistronic ger operons. The Bacillus licheniformis genome contains the gerA family operons gerA, ynd, and ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00451-19

    authors: Aspholm M,Borch-Pedersen K,O'Sullivan K,Fjellheim S,Aardal IB,Granum PE,Lindbäck T

    更新日期:2019-10-04 00:00:00

  • Pyrimidine biosynthetic enzymes of Salmonella typhimurium, repressed specifically by growth in the presence of cytidine.

    abstract::The repressive effects of exogenous cytidine on growing cells was examined in a specially constructed strain in which the pool sizes of endogenous uridine 5'-diphosphate and uridine 5'-triphosphate cannot be varied by the addition of uracil and/or uridine to the medium. Five enzymes of the pyrimidine biosynthetic path...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.124.2.764-774.1975

    authors: Kelln RA,Kinahan JJ,Foltermann KF,O'Donovan GA

    更新日期:1975-11-01 00:00:00

  • PHAGE ISOLATED FROM LYSOGENIC BACILLUS ANTHRACIS.

    abstract::Buck, C. A. (Kansas State University, Manhattan), R. L. Anacker, F. S. Newman, and A. Eisenstark. Phage isolated from lysogenic Bacillus anthracis. J. Bacteriol. 85:1423-1430. 1963.-Bacillus anthracis cultures (a total of 311) were obtained from several laboratories throughout the world; 139 of these were screened for...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.85.6.1423-1430.1963

    authors: BUCK CA,ANACKER RL,NEWMAN FS,EISENSTARK A

    更新日期:1963-06-01 00:00:00

  • Primary characterization of the protein products of the Escherichia coli ompB locus: structure and regulation of synthesis of the OmpR and EnvZ proteins.

    abstract::The ompB operon of Escherichia coli contains the structural genes for two proteins, OmpR and EnvZ, which control the osmoregulated biosynthesis of the porin proteins OmpF and OmpC. By inserting XbaI octamer linkers into the cloned ompB locus, four distinct frameshift mutants were isolated and subsequently characterize...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.164.2.578-584.1985

    authors: Comeau DE,Ikenaka K,Tsung KL,Inouye M

    更新日期:1985-11-01 00:00:00

  • Use of polymyxin B, levallorphan, and tetracaine to isolate novel envelope mutants of Escherichia coli.

    abstract::Mutants of Escherichia coli were isolated by their resistance to the bacteriocidal effects of the membrane-active drugs polymyxin B, levallorphan, and tetracaine. The mutants were examined for additional changes in cellular physiology evoked by the lesions; many polymyxin-resistant strains had a concomitant increased ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.127.2.961-972.1976

    authors: Dame JB,Shapiro BM

    更新日期:1976-08-01 00:00:00

  • Further electron microscope characterization of spore appendages of Clostridium bifermentans.

    abstract::Two distinct spore appendage types of Clostridium bifermentans, a pinlike appendage and a tubular appendage, were studied by electron microscopy. The pinlike appendage is characterized by a shaft, about 100 A in diameter, which has a lobed caplike structure. The tubular appendage, 500 to 600 A in diameter, is characte...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.1.231-238.1968

    authors: Yolton DP,Pope L,Williams MG,Rode LJ

    更新日期:1968-01-01 00:00:00

  • Taxonomy of marine bacteria: Beneckea nigrapulchrituda sp. n.

    abstract::Fourteen bacterial strains of marine origin, which formed colonies containing crystals of a blue-black pigment, were submitted to a morphological, physiological, and nutritional characterization. The results indicated that these organisms form a new species of the genus Beneckea. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.108.3.1380-1383.1971

    authors: Baumann P,Baumann L,Mandel M,Allen RD

    更新日期:1971-12-01 00:00:00