Use of polymyxin B, levallorphan, and tetracaine to isolate novel envelope mutants of Escherichia coli.

abstract：:The staphylococcal virulon is activated by the density-sensing agr system, which is autoinduced by a short peptide (autoinducing peptide [AIP]) processed from a propeptide encoded by agrD. A central segment of the agr locus, consisting of the C-terminal two-thirds of AgrB (the putative processing enzyme), AgrD, and th...

journal_title：Journal of bacteriology

authors： Jarraud S,Lyon GJ,Figueiredo AM,Lina G,Vandenesch F,Etienne J,Muir TW,Novick RP

更新日期：2000-11-01 00:00:00

abstract：:Klebsiella strain RE1544 contains two lac operons, one on the chromosome and one on a lac plasmid. A mutant of RE1544, in which the lacZ genes of both operons produce no active enzyme, was found to synthesize a beta-galactosidase that hydrolyzes ortho-nitrophenyl-beta-D-galactopyranoside but not lactose. Synthesis of ...

journal_title：Journal of bacteriology

authors： Hall BG,Reeve EC

更新日期：1977-10-01 00:00:00

abstract：:We characterized three distinct families of repeated sequences in the genome of the cyanobacterium Calothrix sp. strain PCC 7601. These repeated sequences were present at a level of about 100 copies per Calothrix genome and consisted of tandemly amplified heptanucleotides. These elements were named short tandemly repe...

journal_title：Journal of bacteriology

authors： Mazel D,Houmard J,Castets AM,Tandeau de Marsac N

更新日期：1990-05-01 00:00:00

abstract：:Deletion mutants of plasmid ColE1 that involve the replication origin and adjacent regions of the plasmid have been studied to determine the mechanism by which those mutations affect the expression of plasmid incompatibility. It was observed that (i) a region of ColE1 that is involved in the expression of plasmid inco...

journal_title：Journal of bacteriology

authors： Hashimoto-Gotoh T,Inselburg J

更新日期：1979-08-01 00:00:00

abstract：:The addition of 1 mM orthophenanthroline (OP) into a mating mixture drastically reduced the production of recombinants. Examination of the effect of OP on each step of conjugation showed that the effect on the following steps could not account for the up to 500-fold reduction of recombinant formation: (i) preliminary ...

journal_title：Journal of bacteriology

authors： Ou JT,Reim R

更新日期：1976-10-01 00:00:00

abstract：:SecB is a cytosolic protein required for rapid and efficient export of particular periplasmic and outer membrane proteins in Escherichia coli. SecB promotes export by stabilizing newly synthesized precursor proteins in a nonnative conformation and by targeting the precursors to the inner membrane. Biochemical studies ...

journal_title：Journal of bacteriology

authors： Cook HA,Kumamoto CA

更新日期：1999-05-01 00:00:00

abstract：:We characterized the traU gene of the Escherichia coli K-12 conjugative plasmid F. Plasmids carrying segments of the F transfer operon were tested for their capacity to complement F lac traU526. The protein products of TraU+ clones were identified, and the nucleotide sequence of traU was determined. traU mapped betwee...

journal_title：Journal of bacteriology

authors： Moore D,Maneewannakul K,Maneewannakul S,Wu JH,Ippen-Ihler K,Bradley DE

更新日期：1990-08-01 00:00:00

abstract：:Welch, Ann Marie (U. S. Veterans Administration Hospital, Durham, N. C.). Preliminary survey of fungistatic properties of marine algae. J. Bacteriol. 83:97-99. 1962-Homogenized preparations of 35 marine algae were tested for inhibitory activity against 6 pathogenic or opportunistically pathogenic fungi with saturated ...

journal_title：Journal of bacteriology

authors： WELCH AM

更新日期：1962-01-01 00:00:00

abstract：:A genetic approach was undertaken to identify normal bacterial genes whose products function to limit the effective concentration of antibiotics. In this approach, a multicopy plasmid library containing cloned Escherichia coli chromosomal sequences was screened for transformants that showed increased resistance to a n...

journal_title：Journal of bacteriology

authors： Gambino L,Gracheck SJ,Miller PF

更新日期：1993-05-01 00:00:00

abstract：:The reactive alpha-oxoaldehydes such as glyoxal (GO) and methylglyoxal (MG) are generated in vivo from sugars through oxidative stress. GO and MG are believed to be removed from cells by glutathione-dependent glyoxalases and other aldehyde reductases. We isolated a number of GO-resistant (GO(r)) mutants from Escherich...

journal_title：Journal of bacteriology

authors： Lee C,Kim I,Lee J,Lee KL,Min B,Park C

更新日期：2010-08-01 00:00:00

abstract：:A library of Zymomonas mobilis genomic DNA was constructed in the broad-host-range cosmid pLAFR1. The library was mobilized into a variety of Escherichia coli and Pseudomonas putida trp mutants by using the helper plasmid pRK2013. Five Z. mobilis trp genes were identified by the ability to complement the trp mutants. ...

journal_title：Journal of bacteriology

authors： Eddy CK,Smith OH,Noel KD

更新日期：1988-07-01 00:00:00

abstract：:Freeze-etching was applied to preparations, with and without glycerol, of Acinetobacter sp. strain MJT/F5/199A, consisting of intact cells after normal growth or after incubation with chloramphenicol, spheroplasts, and isolated cell walls and outer membranes. Etched preparations show that a regular array of subunits f...

journal_title：Journal of bacteriology

authors： Sleytr UB,Thornley MJ

更新日期：1973-12-01 00:00:00

abstract：:Neely, W. Brock (The Dow Chemical Co., Midland, Mich.). Action of formaldehyde on microorganisms. I. Correlation of activity with formaldehyde metabolism. J. Bacteriol. 85:1028-1031. 1963.-The action of formaldehyde in concentrations sublethal for the culture appeared to be at least twofold. The first phase caused a d...

journal_title：Journal of bacteriology

authors： NEELY WB

更新日期：1963-05-01 00:00:00

abstract：:Spontaneous mutators of Salmonella typhimurium LT2 were generated by inserting the transposable element Tn5 or Tn10 into the bacterial chromosome. Two mutators mapped at the position of the mutH and mutL loci of S. typhimurium, and two other mutators mapped at positions corresponding to the mutS and uvrD loci of Esche...

journal_title：Journal of bacteriology

authors： Shanabruch WG,Behlau I,Walker GC

更新日期：1981-09-01 00:00:00

abstract：:The effects of high doses of radiation (1, 5, or 20 Mrad) on the toxicity, pyrogenicity, and immunogenicity of Salmonella typhimurium cells and endotoxin were studied. Toxicity decreased progressively after exposure to 1, 5, or 20 Mrad. The lethal effect of 1-Mrad exposed cells was greater than that of heat-, acetone-...

journal_title：Journal of bacteriology

authors： Previte JJ

更新日期：1968-06-01 00:00:00

abstract：:The bacteriophage T4 MotA protein is a transcriptional activator of T4-modified host RNA polymerase and is required for activation of the middle class of T4 promoters. MotA alone binds to the -30 region of T4 middle promoters, a region that contains the MotA box consensus sequence [(t/a)(t/a)TGCTT(t/c)A]. We report th...

journal_title：Journal of bacteriology

authors： Gerber JS,Hinton DM

更新日期：1996-11-01 00:00:00

abstract：:Chlamydiae are unusual obligately intracellular bacteria that do not synthesize detectable peptidoglycan. However, they possess genes that appear to encode products with peptidoglycan biosynthetic activity. Bioinformatic analysis predicts that chlamydial MurE possesses UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelic acid (...

journal_title：Journal of bacteriology

authors： Patin D,Bostock J,Blanot D,Mengin-Lecreulx D,Chopra I

更新日期：2009-12-01 00:00:00

abstract：:Undecyl acetate esterase has been purified from Pseudomonas cepacia grown on the methyl ketone, 2-tridecanone. The K(m) for undecyl acetate was 2.3 x 10(-2) M. Polyacrylamide gel electrophoresis indicated that two esterase bands were being recovered during purification. These bands were separated by preparative polyac...

journal_title：Journal of bacteriology

authors： Shum AC,Markovetz AJ

更新日期：1974-06-01 00:00:00

abstract：:The two-partner secretion system ExlBA, expressed by strains of Pseudomonas aeruginosa belonging to the PA7 group, induces hemorrhage in lungs due to disruption of host cellular membranes. Here we demonstrate that the exlBA genes are controlled by a pathway consisting of cAMP and the virulence factor regulator (Vfr). ...

journal_title：Journal of bacteriology

authors： Berry A,Han K,Trouillon J,Robert-Genthon M,Ragno M,Lory S,Attrée I,Elsen S

更新日期：2018-05-24 00:00:00

abstract：:Vegetative cells and cysts of Azotobacter vinelandii 12837 were prepared for electron microscopy by several methods assumed to preserve structural details destroyed by techniques previously reported in the literature. Examination of large numbers of cells and cysts by these methods revealed four structural details not...

journal_title：Journal of bacteriology

authors： Vela GR,Cagle GD,Holmgren PR

更新日期：1970-11-01 00:00:00

abstract：:Two solid medium formulations, designated 100:10 and 10:10, were developed for the growth of Thiobacillus ferrooxidans. The new media contain a mixture of both ferrous iron and thiosulfate as available energy sources, permitting the detection of colony morphology variants that arise spontaneously in a wild-type popula...

journal_title：Journal of bacteriology

authors： Schrader JA,Holmes DS

更新日期：1988-09-01 00:00:00

abstract：:The maltose regulon consists of three operons controlled by a positive regulatory gene, malT. Deletions of the gene crp were introduced into strains which carried a malT-lacZ hybrid gene. From the observed reduction in beta-galactosidase activity it was concluded that the expression of malT-lacZ, and therefore of malT...

journal_title：Journal of bacteriology

authors： Chapon C

更新日期：1982-05-01 00:00:00

abstract：:Salmonella virulence requires the initial invasion of host cells, followed by modulation of the intracellular environment for survival and replication. In an effort to characterize the role of small RNAs in Salmonella pathogenesis, we inadvertently identified a 5-kDa protein named YshB that is involved in the intracel...

journal_title：Journal of bacteriology

authors： Bomjan R,Zhang M,Zhou D

更新日期：2019-08-08 00:00:00

abstract：:Treatment of pneumococci with activator (a protein that induces bacterial "competence" to absorb deoxyribonucleic acid molecules and undergo genetic transformation) can cause either protoplast formation or leakage of intracellular components to the medium depending on postincubation conditions. The leaked intracellula...

journal_title：Journal of bacteriology

authors： Seto H,Tomasz A

更新日期：1975-01-01 00:00:00

abstract：:Plasmid Rms312, specifying resistance to tetracycline (Tc), chloramphenicol (Cm), streptomycin (Sm), sulfonamide (Su), and mercury chloride (Mer), deletes both Tc and Cm Sm Su Mer determinants at a high frequency in Salmonella typhimurium LT2. S. typhimurium mutants that were stable carriers of Rms312 were isolated by...

journal_title：Journal of bacteriology

authors： Watanabe H,Hashimoto H,Mitsuhashi S

更新日期：1980-04-01 00:00:00

abstract：:Five transposon Tn5 mutants of the procaryote Myxococcus xanthus had been shown previously to be defective in lipopolysaccharide biosynthesis (J. M. Fink,-M. Kalos, and J. F. Zissler, J. Bacteriol. 171:2033-2041, 1989). These mutants were studied for possible defects in gliding motility and multicellular development. ...

journal_title：Journal of bacteriology

authors： Fink JM,Zissler JF

更新日期：1989-04-01 00:00:00

abstract：:Adaptation of Lactococcus lactis towards progressive carbon starvation is mediated by three different types of transcriptomic responses: (i) global responses, i.e., general decreases of functions linked to bacterial growth and lack of induction of the general stress response; (ii) specific responses functionally relat...

journal_title：Journal of bacteriology

authors： Redon E,Loubiere P,Cocaign-Bousquet M

更新日期：2005-05-01 00:00:00

abstract：:Streptomyces clavuligerus produces at least five different clavam metabolites, including clavulanic acid and the methionine antimetabolite, alanylclavam. In vitro transposon mutagenesis was used to analyze a 13-kb region upstream of the known paralogue gene cluster. The paralogue cluster includes one group of clavulan...

journal_title：Journal of bacteriology

authors： Zelyas NJ,Cai H,Kwong T,Jensen SE

更新日期：2008-12-01 00:00:00

abstract：:The Bacillus subtilis SpoVE integral membrane protein is essential for the heat resistance of spores, probably because of its involvement in spore peptidoglycan synthesis. We found that an SpoVE-yellow fluorescent protein (YFP) fusion protein becomes localized to the forespore during the earliest stages of engulfment,...

journal_title：Journal of bacteriology

authors： Real G,Fay A,Eldar A,Pinto SM,Henriques AO,Dworkin J

更新日期：2008-01-01 00:00:00

abstract：:In stationary phase, 95% of the fructosyltransferase (FTase) activity of Streptococcus salivarius ATCC 25975 was found associated with the cells. Within the first 15 min after inoculation into fresh medium, the specific activity of cell-associated FTase decreased by 92% of its initial value. After this period of initi...

journal_title：Journal of bacteriology

authors： Jacques NA,Wittenberger CL

更新日期：1981-12-01 00:00:00