Spontaneous mutators of salmonella typhimurium LT2 generated by insertion of transposable elements.

abstract：:Neonatal bacterial meningitis continues to be an important cause of mortality and morbidity worldwide. Escherichia coli possessing the K1 capsular polysaccharide is the most common Gram-negative pathogen causing neonatal meningitis. Here we present the complete genome sequence of neonatal meningitis-associated E. coli...

journal_title：Journal of bacteriology

authors： Lu S,Zhang X,Zhu Y,Kim KS,Yang J,Jin Q

更新日期：2011-12-01 00:00:00

abstract：:The endophytic diazotroph Gluconacetobacter diazotrophicus secretes a constitutively expressed levansucrase (LsdA, EC 2.4.1.10) to utilize plant sucrose. LsdA, unlike other extracellular levansucrases from gram-negative bacteria, is transported to the periplasm by a signal-peptide-dependent pathway. We identified an u...

journal_title：Journal of bacteriology

authors： Arrieta JG,Sotolongo M,Menéndez C,Alfonso D,Trujillo LE,Soto M,Ramírez R,Hernández L

更新日期：2004-08-01 00:00:00

abstract：:It has been previously established that Thiobacillus neapolitanus fixes CO2 by using a form I ribulose bisphosphate carboxylase/oxygenase (RuBisCO), that much of the enzyme is sequestered into carboxysomes, and that the genes for the enzyme, cbbL and cbbS, are part of a putative carboxysome operon. In the present stud...

journal_title：Journal of bacteriology

authors： Baker SH,Jin S,Aldrich HC,Howard GT,Shively JM

更新日期：1998-08-01 00:00:00

abstract：:Gaffar, Abdul (Brigham Young University, Provo, Utah), David R. Terry, and Richard D. Sagers. Amino acid composition of walls from single and filamentous cells of Clostridium acidiurici. J. Bacteriol. 91:1618-1624. 1966.-The walls from single and filamentous cells of Clostridium acidiurici were shown to contain 11 ami...

journal_title：Journal of bacteriology

authors： Gaffar A,Terry DR,Sagers RD

更新日期：1966-04-01 00:00:00

abstract：:Microbacterium barkeri strain 2011-R4 is a Gram-positive epiphyte which has been confirmed as a biocontrol agent against several plant pathogens in our previous studies. Here, we present the draft genome sequence of this strain, which was isolated from the rice rhizosphere in Tonglu city, Zhejiang province, China. ...

journal_title：Journal of bacteriology

authors： Liu J,Zhou Q,Ibrahim M,Liu H,Jin G,Zhu B,Xie G

更新日期：2012-12-01 00:00:00

abstract：:To understand further the role of the flagellum of Vibrio anguillarum in virulence, invasive and adhesive properties of isogenic motility mutants were analyzed by using a chinook salmon embryo cell line. Adhesion was unaffected but invasion of the cell line was significantly decreased in nonmotile or partially motile ...

journal_title：Journal of bacteriology

authors： Ormonde P,Hörstedt P,O'Toole R,Milton DL

更新日期：2000-04-01 00:00:00

abstract：:Restriction fragments containing the gene encoding cyanase, cynS, without its transcriptional regulatory sequences were placed downstream of lac and tac promoters in various pUC derivatives to maximize production of cyanase. Plasmid pSJ105, which contains the cynS gene and an upstream open reading frame, gave the high...

journal_title：Journal of bacteriology

authors： Sung YC,Anderson PM,Fuchs JA

更新日期：1987-11-01 00:00:00

abstract：:The gene coding for sorbitol dehydrogenase (SDH) of Rhodobacter sphaeroides Si4 was located 55 nucleotides upstream of the mannitol dehydrogenase gene (mtlK) within a previously unrecognized polyol operon. This operon probably consists of all the proteins necessary for transport and metabolization of various polyols. ...

journal_title：Journal of bacteriology

authors： Stein MA,Schäfer A,Giffhorn F

更新日期：1997-10-01 00:00:00

abstract：:West Indian spiny lobsters, Panulirus argus, synthesized a hemolymph bactericidin after being injected with killed suspensions of gram-negative bacillus EMB-1 isolated from the normal gut of this lobster. To study differences between the primary response and secondary response, animals were given a primary antigen inj...

journal_title：Journal of bacteriology

authors： Evans EE,Weinheimer PF,Painter B,Acton RT,Evans ML

更新日期：1969-06-01 00:00:00

abstract：:A reaction center H- strain (RCH-) of Rhodobacter sphaeroides, PUHA1, was made by in vitro deletion of an XhoI restriction endonuclease fragment from the puhA gene coupled with insertion of a kanamycin resistance gene cartridge. The resulting construct was delivered to R. sphaeroides wild-type 2.4.1, with the defectiv...

journal_title：Journal of bacteriology

authors： Sockett RE,Donohue TJ,Varga AR,Kaplan S

更新日期：1989-01-01 00:00:00

abstract：:The interaction of Streptococcus pyogenes (group A streptococcus [GAS]) with its human host requires several surface proteins. In this study, we isolated mutations in a gene required for the surface localization of protein F by transposon mutagenesis of the M6 strain JRS4. This gene (srtA) encodes a protein homologous...

journal_title：Journal of bacteriology

authors： Barnett TC,Scott JR

更新日期：2002-04-01 00:00:00

abstract：:A method was devised for the isolation of yeast monomorphic (LEV) mutants of Candida albicans. By this procedure, about 20 stable yeast-like mutants were isolated after mutagenesis with ethyl methane sulfonate. The growth rate of the mutants in different carbon sources, both fermentable and not, was indistinguishable ...

journal_title：Journal of bacteriology

authors： Elorza MV,Sentandreu R,Ruiz-Herrera J

更新日期：1994-04-01 00:00:00

abstract：:The bfe locus codes for the cell surface receptor for vitamin B12, the E colicins, and bacteriophage BF23 in the Escherichia coli outer membrane. When the bfe+ allele, which is closely linked to the argH locus, was introduced into an argH bfe recipient by conjugation, arg+ recombinant cells rapidly and simultaneously ...

journal_title：Journal of bacteriology

authors： Bassford PJ Jr,Kadner RJ,Schnaitman CA

更新日期：1977-01-01 00:00:00

abstract：:Wilson, Dwight E. (Rensselaer Polytechnic Institute, Troy, N.Y.). Fractionation of Newcastle disease virus by chromatography on diethylaminoethyl cellulose. J. Bacteriol. 84:295-301. 1962.-The L. Kansas and NK strains of Newcastle disease virus were chromatographed on diethylaminoethyl (DEAE) cellulose ion-exchange co...

journal_title：Journal of bacteriology

authors： WILSON DE

更新日期：1962-08-01 00:00:00

abstract：:Spiralin could not be solubilized in the absence of detergents, and it was shown by charge-shift crossed immunoelectrophoresis that this protein was capable of binding detergents under nondenaturing conditions. These properties indicate the amphiphilic nature of spiralin, which therefore should be regarded as an intri...

journal_title：Journal of bacteriology

authors： Wróblewski H

更新日期：1979-11-01 00:00:00

abstract：:Spheroplasts of two different auxotrophic strains of Saccharomyces cerevisiae, both of mating type a, were fused with the aid of polyethylene glycol and calcium ions. After reversion to vegetative cells in solid media, the resulting zygotes were shown to be diploid cells of mating type aa. ...

journal_title：Journal of bacteriology

authors： van Solingen P,van der Plaat JB

更新日期：1977-05-01 00:00:00

abstract：:Picolinic acid (PA) is a natural toxic pyridine derivative. Microorganisms can degrade and utilize PA for growth. However, the full catabolic pathway of PA and its physiological and genetic foundation remain unknown. In this study, we identified a gene cluster, designated picRCEDFB4B3B2B1A1A2A3, responsible for the de...

journal_title：Journal of bacteriology

authors： Qiu J,Zhao L,Xu S,Chen Q,Chen L,Liu B,Hong Q,Lu Z,He J

更新日期：2019-07-24 00:00:00

abstract：:We identified Mg2+-responsive promoters of the phoPQ, mgtA, and mgrB genes of Escherichia coli K-12 by S1 nuclease analysis. Expression of these genes was induced by magnesium limitation and depended on PhoP and PhoQ. The transcription start sites were also determined, which allowed us to find a (T/G)GTTTA direct repe...

journal_title：Journal of bacteriology

authors： Kato A,Tanabe H,Utsumi R

更新日期：1999-09-01 00:00:00

abstract：:The Rnf complex is a respiratory enzyme that catalyzes the oxidation of reduced ferredoxin to the reduction of NAD+, and the negative free energy change of this reaction is used to generate a transmembrane ion gradient. In one class of anaerobic acetogenic bacteria, the Rnf complex is believed to be essential for ener...

journal_title：Journal of bacteriology

authors： Westphal L,Wiechmann A,Baker J,Minton NP,Müller V

更新日期：2018-10-10 00:00:00

abstract：:Archaeal transcriptional machinery is similar to that of eukaryotes. We studied the fates of various components of the Sulfolobus solfataricus transcriptional apparatus under different stresses and found that in cells incubated at 90 degrees C for 1 h, transcription factor E (TFE) is selectively depleted, but its mRNA...

journal_title：Journal of bacteriology

authors： Iqbal J,Qureshi SA

更新日期：2010-06-01 00:00:00

abstract：:Biochemical events which occur during macroconidial germination have been studied in the dermatophyte Microsporum gypseum. The specific activity levels of various metabolic enzymes have been assayed during germination time periods. The accumulated levels of several of these enzymes, as a function of exogenous carbohyd...

journal_title：Journal of bacteriology

authors： Leighton TJ,Stock JJ,Kelln RA

更新日期：1970-08-01 00:00:00

abstract：:A nonswarming Serratia liquefaciens mutant deficient in serrawettin W2 production was constructed by transposon mutagenesis. Sequence homology indicated that insertion had occurred in gene swrA, which encodes a putative peptide synthetase. Expression of swrA is controlled by quorum sensing. ...

journal_title：Journal of bacteriology

authors： Lindum PW,Anthoni U,Christophersen C,Eberl L,Molin S,Givskov M

更新日期：1998-12-01 00:00:00

abstract：:pBL2 was identified genetically but not physically in Streptomyces lividans after its mating with S. bambergiensis. During conjugation, pBL2 was transferred at high frequency to S. lividans and S. coelicolor. pBL2.1 DNA isolated from S. coelicolor exconjugants as a circular plasmid was shown to derive from the genome ...

journal_title：Journal of bacteriology

authors： Zotchev SB,Schrempf H,Hutchinson CR

更新日期：1995-08-01 00:00:00

abstract：:ToxR, the transmembrane regulatory protein required for expression of virulence factors in the human diarrheal pathogen Vibrio cholerae, directly activates and represses the transcription of two outer membrane porins, OmpU and OmpT, respectively. In an attempt to dissect the role of the OmpU and OmpT porins in viabili...

journal_title：Journal of bacteriology

authors： Provenzano D,Lauriano CM,Klose KE

更新日期：2001-06-01 00:00:00

abstract：:Cyanobacteria are monophyletic organisms that perform oxygenic photosynthesis. While they exhibit great diversity, they have a common set of genes. However, the essentiality of them for viability has hampered the elucidation of their functions. One example of these genes is cyabrB1 (also known as calA in Anabaena sp. ...

journal_title：Journal of bacteriology

authors： Higo A,Nishiyama E,Nakamura K,Hihara Y,Ehira S

更新日期：2019-08-08 00:00:00

abstract：:Cells of the osmotolerant yeast Saccharomyces rouxii were transformed to protoplasts in good yield (85%) by digesting cell walls with snail-gut enzyme in the presence of 10 mM dithioerythritol, 0.1 M sodium phosphate buffer (pH 6.8), and 2.0 M KCl. The requirement for 2.0 M KCl compares with that for S. bisporus var. ...

journal_title：Journal of bacteriology

authors： Arnold WN,Garrison RG

更新日期：1979-03-01 00:00:00

abstract：:A class II restriction endonuclease which recognizes the same nucleotide sequence as EndoR.HaeIII has been found in four of seven isolates of Neisseria gonorrhoeae tested. ...

journal_title：Journal of bacteriology

authors： Clanton DJ,Woodward JM,Miller RV

更新日期：1978-07-01 00:00:00

abstract：:A human isolate of Salmonella enteritidis which displayed strong pellicle formation during static broth culture and mannose-sensitive hemagglutination produced fimbriae which were morphologically indistinguishable from type 1 fimbriae of members of the family Enterobacteriaceae. Fimbrin was purified to homogeneity, an...

journal_title：Journal of bacteriology

authors： Feutrier J,Kay WW,Trust TJ

更新日期：1986-10-01 00:00:00

abstract：:Shigella spp. are the major cause of bacillary dysentery worldwide. The pathogenic process involves bacterial invasion and lysis of the phagocytic vacuole, followed by replication and movement within the cell cytoplasm and, ultimately, spread directly into adjacent cells. This study demonstrates that S. flexneri cytoc...

journal_title：Journal of bacteriology

authors： Way SS,Sallustio S,Magliozzo RS,Goldberg MB

更新日期：1999-02-01 00:00:00

abstract：:The bacteriophage Mu genome contains a centrally located strong gyrase site (SGS) that is required for efficient prophage replication. To aid in studying the unusual properties of the SGS, we sought other gyrase sites that might be able to substitute for the SGS in Mu replication. Five candidate sites were obtained by...

journal_title：Journal of bacteriology

authors： Oram M,Pato ML

更新日期：2004-07-01 00:00:00