Identification of a new sequence-specific endonuclease, NgoII, from Neisseria gonorrhoeae.

abstract：:Two low-molecular-weight basic proteins, termed A and B proteins, comprise about 15% of the protein of dormant spores of Bacillus megaterium. Irradiation of intact dormant spores with ultraviolet light results in covalent cross-linking of the A and B proteins to other spore macromolecules. The cross-linked A and B pro...

journal_title：Journal of bacteriology

authors： Setlow B,Setlow P

更新日期：1979-08-01 00:00:00

abstract：:Hottle, G. A. (Naval Biological Laboratory, University of California, Berkeley), and D. N. Wright. Growth and survival of Mycoplasma neurolyticum in liquid media. J. Bacteriol. 91:1834-1839. 1966.-Maximal growth of Mycoplasma neurolyticum (between 10(8) and 10(9) colony-forming units per ml) was obtained after 3 days ...

journal_title：Journal of bacteriology

authors： Hottle GA,Wright DN

更新日期：1966-05-01 00:00:00

abstract：:Upon fractionating Saccharomyces cerevisiae asynchronous cultures by sucrose density gradient centrifugation in a zonal rotor and examining the exo-1,3-beta-glucanase and deoxyribonucleic acid content of the cells, a periodic step increase in the activity of this enzyme was observed, indicating a discontinuous pattern...

journal_title：Journal of bacteriology

authors： del Rey F,Santos T,García-Acha I,Nombela C

更新日期：1979-09-01 00:00:00

abstract：:Biofilms contribute to virulence of Staphylococcus aureus. Formation of biofilms is multifactorial, involving polysaccharide, protein, and DNA components, which are controlled by various regulators. Here we report that deletion of the rsp gene resulted in an increase in biofilm formation in strain MW2, suggesting that...

journal_title：Journal of bacteriology

authors： Lei MG,Cue D,Roux CM,Dunman PM,Lee CY

更新日期：2011-10-01 00:00:00

abstract：:Helicobacter pylori urease requires nickel ions in the enzyme active site for catalytic activity. Nickel ions must, therefore, be actively acquired by the bacterium. NixA (high-affinity nickel transport protein)-deficient mutants of H. pylori retain significant urease activity, suggesting the presence of alternate nic...

journal_title：Journal of bacteriology

authors： Hendricks JK,Mobley HL

更新日期：1997-09-01 00:00:00

abstract：:Strains of Staphylococcus aureus, an important human pathogen, display up to 20% variability in their genome sequence, and most sequence information is available for human clinical isolates that have not been subjected to genetic analysis of virulence attributes. S. aureus strain Newman, which was also isolated from a...

journal_title：Journal of bacteriology

authors： Baba T,Bae T,Schneewind O,Takeuchi F,Hiramatsu K

更新日期：2008-01-01 00:00:00

abstract：:The enzyme phosphatidylglycerolphosphate synthase (PGPS; CDP-diacylglycerol glycerol 3-phosphate 3-phosphatidyltransferase; EC 2.7.8.5) catalyzes the committed step in the cardiolipin biosynthetic pathway. To study the regulation of PGPS in Schizosaccharomyces pombe, we characterized the enzyme biochemically. Maximum ...

journal_title：Journal of bacteriology

authors： Karkhoff-Schweizer RR,Kelly BL,Greenberg ML

更新日期：1991-10-01 00:00:00

abstract：:Many DNA viruses concatemerize their genomes as a prerequisite to packaging into capsids. Concatemerization arises from either replication or homologous recombination. Replication is already the target of many antiviral drugs, and viral recombinases are an attractive target for drug design, particularly for combinatio...

journal_title：Journal of bacteriology

authors： Vellani TS,Myers RS

更新日期：2003-04-01 00:00:00

abstract：:A mutant of Escherichia coli is described which is defective in the conversion of arginine to putrescine. The activity of the enzyme agmatine ureohydrolase is greatly reduced, whereas the activity of the other two enzymes of the pathway, the constitutive arginine decarboxylase and the inducible arginine decarboxylase,...

journal_title：Journal of bacteriology

authors： Hirshfield IN,Rosenfeld HJ,Leifer Z,Maas WK

更新日期：1970-03-01 00:00:00

abstract：:A comparison of the spectra of spontaneous growth-dependent and adaptive mutations in ebgR shows that both spectra are dominated by insertion sequence (IS)-mediated mutations. The difference between growth-dependent mutations (61% IS mediated) and adaptive mutations (80% IS mediated) is highly significant (P < 0.0001)...

journal_title：Journal of bacteriology

authors： Hall BG

更新日期：1999-02-01 00:00:00

abstract：:Lactobacillus pentosus MP-10 was isolated from brines of naturally fermented Aloreña green table olives. MP-10 has potential probiotic traits, including inhibition of human pathogenic bacteria, survival at low pH (1.5), and bile salt tolerance (3%). Here, we report for the first time the annotated genome sequence of L...

journal_title：Journal of bacteriology

authors： Abriouel H,Benomar N,Pulido RP,Cañamero MM,Gálvez A

更新日期：2011-09-01 00:00:00

abstract：:Under static growth conditions, hyperpiliated, nontwitching pilT and pilU mutants of Pseudomonas aeruginosa formed dense biofilms, showing that adhesion, not twitching motility, is necessary for biofilm initiation. Under flow conditions, the pilT mutant formed mushroom-like structures larger than those of the wild typ...

journal_title：Journal of bacteriology

authors： Chiang P,Burrows LL

更新日期：2003-04-01 00:00:00

abstract：:Flac mutants have been isolated in Escherichia coli K-12 which carry dominant mutations resulting in insensitivity to transfer inhibition by the Fin(+) I-like plasmid R62. These mutants were still sensitive to transfer inhibition by the fin(+) F-like plasmid R100 and, conversely, FlactraO(-) and traP(-) mutants, which...

journal_title：Journal of bacteriology

authors： Willetts NS,Paranchych W

更新日期：1974-10-01 00:00:00

abstract：:IS630 is a 1.15-kilobase sequence in Shigella sonnei that, unlike many mobile elements, seems not to mediate cointegration between different replicons. To assess its transposition, we constructed composite elements containing inverted copies of IS630 flanking a drug resistance gene. We found that these composite eleme...

journal_title：Journal of bacteriology

authors： Tenzen T,Matsutani S,Ohtsubo E

更新日期：1990-07-01 00:00:00

abstract：:The 56-kb class II toluene catabolic transposon Tn4651 from Pseudomonas putida plasmid pWW0 is unique in that (i) its efficient resolution requires, in addition to the 0.2-kb resolution (res) site, the two gene products TnpS and TnpT and (ii) the 2.4-kb tnpT-res-tnpS region is 48 kb apart from the tnpA gene (M. Tsuda,...

journal_title：Journal of bacteriology

authors： Genka H,Nagata Y,Tsuda M

更新日期：2002-09-01 00:00:00

abstract：:Three polynucleotide phosphorylase mutations, isolated in heavily mutagenized Escherichia coli strains Q7, Q13, and Q27, were characterized after their transfer by P1 transduction to nearly isogenic strains which lack ribonuclease I. Each strain has a different altered form of polynucleotide phosphorylase. One enzyme ...

journal_title：Journal of bacteriology

authors： Reiner AM

更新日期：1969-03-01 00:00:00

abstract：:We have isolated transposon insertions in the lacA gene encoding an endogenous beta-galactosidase of Bacillus subtilis. Upstream of the putative operon containing lacA is a negative regulator, lacR, which encodes a product related to a family of regulators that includes the lactose repressor, lacI, of Escherichia coli...

journal_title：Journal of bacteriology

authors： Daniel RA,Haiech J,Denizot F,Errington J

更新日期：1997-09-01 00:00:00

abstract：:Mutations in carbohydrate-negative mutants of Pseudomonas aeruginosa PAO1 individually deficient in glucose 6-phosphate dehydrogenase (zwf), 6-phosphogluconate dehydratase (edd), or pyruvate carboxylase (pyc) were mapped on the chromosome by plasmid R68.45-mediated conjugation and by bacteriophage F116L-mediated trans...

journal_title：Journal of bacteriology

authors： Roehl RA,Feary TW,Phibbs PV Jr

更新日期：1983-12-01 00:00:00

abstract：:Preparations of membrane plus wall derived from Bacillus subtilis W23 were used to study the in vitro synthesis of peptidoglycan and teichoic acid and their linkage to the preexisting cell wall. The teichoic acid synthesis showed an ordered requirement for the incorporation of N-acetylglucosamine from uridine 5'-dipho...

journal_title：Journal of bacteriology

authors： Wyke AW,Ward JB

更新日期：1977-06-01 00:00:00

abstract：:Mevalonate (MVA) metabolism provides the isoprenoids used in archaeal lipid biosynthesis. In synthesis of isopentenyl diphosphate, the classical MVA pathway involves decarboxylation of mevalonate diphosphate, while an alternate pathway has been proposed to involve decarboxylation of mevalonate monophosphate. To identi...

journal_title：Journal of bacteriology

authors： Vannice JC,Skaff DA,Keightley A,Addo JK,Wyckoff GJ,Miziorko HM

更新日期：2014-03-01 00:00:00

abstract：:Staphylococcus lugdunensis is the only coagulase-negative Staphylococcus species with a locus encoding iron-regulated surface determinant (Isd) proteins. In Staphylococcus aureus, the Isd proteins capture heme from hemoglobin and transfer it across the wall to a membrane-bound transporter, which delivers it into the c...

journal_title：Journal of bacteriology

authors： Zapotoczna M,Heilbronner S,Speziale P,Foster TJ

更新日期：2012-12-01 00:00:00

abstract：:Manganese is accumulated in Bacillus subtilis by a highly specific active transport system. This trace element "pump" is insensitive to added magnesium or calcium and preferentially accumulates manganese in the presence of cobalt, iron, and copper. Manganese uptake in B. subtilis is inhibited by cyanide, azide, pentac...

journal_title：Journal of bacteriology

authors： Eisenstadt E,Fisher S,Der CL,Silver S

更新日期：1973-03-01 00:00:00

abstract：:The human bacterial pathogens Chlamydia spp. possess a genus-specific lipopolysaccharide as a major surface antigen, the structure of which has been determined by analytical chemistry as Kdop alpha 2-8-Kdop alpha 2-4-Kdop alpha 2-6GlcNp beta 1-6-GlcNol (Kdo, 3-deoxy-D-manno-2-octulosonic acid). Immunochemical studies ...

journal_title：Journal of bacteriology

authors： Holst O,Brade L,Kosma P,Brade H

更新日期：1991-03-01 00:00:00

abstract：:Tn4551, a clindamycin resistance (Ccr) transposon from the R plasmid pBI136, was cloned onto an Escherichia coli-Bacteroides shuttle vector which could replicate normally in E. coli but was maintained unstably in Bacteroides fragilis. To aid in cloning and to ensure maintenance of Tn4551 in E. coli, a kanamycin resist...

journal_title：Journal of bacteriology

authors： Smith CJ,Spiegel H

更新日期：1987-08-01 00:00:00

abstract：:Several lysosomal glycosidase activities were examined in vitro during heat-induced germination of Dictyostelium discoideum spores and were found not to be coordinately controlled. The level of beta-glucosidase activity increased significantly during the emergence stage of germination. Both alpha-glucosidase and N-ace...

journal_title：Journal of bacteriology

authors： Tisa LS,Cotter DA

更新日期：1980-02-01 00:00:00

abstract：:Escherichia coli outer membrane protein E was purified, and its amino acid composition and N-terminal amino acid were determined. The purified protein was shown to be immunologically and electrophoretically identical to proteins Ic (U. Henning, W. Schmidmayr, and I. Hindennach, Mol. Gen. Genet. 154:293-298, 1977) and ...

journal_title：Journal of bacteriology

authors： Chai TJ,Foulds J

更新日期：1979-08-01 00:00:00

abstract：:Photobacterium leiognathi ATCC 25521 (the type strain and light-organ symbiont of ponyfish) is one of the few bacteria that produces a copper-zinc superoxide dismutase, termed bacteriocuprein. We enzymologically and immunologically characterized the bacteriocuprein superoxide dismutases in sonicates from the type stra...

journal_title：Journal of bacteriology

authors： Dunlap PV,Steinman HM

更新日期：1986-02-01 00:00:00

abstract：:While the protein complex responsible for controlling the direction (clockwise [CW] or counterclockwise [CCW]) of flagellar rotation has been fairly well studied in Escherichia coli and Salmonella, less is known about the switch complex in Bacillus subtilis or other Gram-positive species. Two component proteins (FliG ...

journal_title：Journal of bacteriology

authors： Ward E,Kim EA,Panushka J,Botelho T,Meyer T,Kearns DB,Ordal G,Blair DF

更新日期：2019-03-26 00:00:00

abstract：:The flaW, flaU, and flaV genes of Salmonella typhimurium LT2 were cloned into pBR322. These genes were mapped on the cloned DNA fragments by restriction endonuclease analysis and construction of the deletion derivatives. Their gene products were identified, by the minicell method, as proteins whose molecular weights w...

journal_title：Journal of bacteriology

authors： Homma M,Kutsukake K,Iino T

更新日期：1985-08-01 00:00:00

abstract：:The phoP-phoQ-regulated pagC locus is essential for full virulence and survival within macrophages of Salmonella typhimurium. The protein product, DNA sequence, and transcript of pagC were determined. The pagC locus encodes a single 188-amino-acid membrane protein that is similar to the ail-encoded eucaryotic cell inv...

journal_title：Journal of bacteriology

authors： Pulkkinen WS,Miller SI

更新日期：1991-01-01 00:00:00