Site-specific recombination system encoded by toluene catabolic transposon Tn4651.

abstract：:Previously, we identified a gene (aldA) from Myxococcus xanthus, which we suggested encoded the enzyme alanine dehydrogenase on the basis of similarity to known Ald protein sequences (M. J. Ward, H. Lew, A. Treuner-Lange, and D. R. Zusman, J. Bacteriol. 180:5668-5675, 1998). In this study, we have confirmed that aldA ...

journal_title：Journal of bacteriology

authors： Ward MJ,Lew H,Zusman DR

更新日期：2000-01-01 00:00:00

abstract：:In this study, we demonstrated the remarkable genome plasticity of lytic lactococcal phages that allows them to rapidly adapt to the dynamic dairy environment. The lytic double-stranded DNA phage ul36 was used to sequentially infect a wild-type strain of Lactococcus lactis and two isogenic derivatives with genes encod...

journal_title：Journal of bacteriology

authors： Labrie SJ,Moineau S

更新日期：2007-02-01 00:00:00

abstract：:The decline in colony-forming ability observed during tryptophan starvation of Bacillus subtilis auxotrophs is a concentration-dependent phenomenon. It does not manifest itself when the initial cell concentration is 10(6) cells/ml or lower. This property has been used to test the killing activity of different fraction...

journal_title：Journal of bacteriology

authors： Barlati S,Majerfeld I

更新日期：1970-02-01 00:00:00

abstract：:During insect infection Photorhabdus luminescens emits light and expresses virulence factors, including insecticidal toxin complexes (Tcs) and an RTX-like metalloprotease (Prt). Using quantitative PCR and protein assays, we describe the expression patterns of these factors both in culture and during insect infection a...

journal_title：Journal of bacteriology

authors： Daborn PJ,Waterfield N,Blight MA,Ffrench-Constant RH

更新日期：2001-10-01 00:00:00

abstract：:We investigated the effect of time, temperature, and the presence of sodium chloride, nitrates, and nitrites in the medium on the growth and production of enterotoxin B by Staphylococcus aureus. Assays by the double gel-diffusion method showed that maximal enterotoxin B production occurs at the beginning of the statio...

journal_title：Journal of bacteriology

authors： McLean RA,Lilly HD,Alford JA

更新日期：1968-04-01 00:00:00

abstract：:Lag is a temporary period of nonreplication seen in bacteria that are introduced to new media. Despite latency being described by Müller in 1895, only recently have we gained insights into the cellular processes characterizing lag phase. This review covers literature to date on the transcriptomic, proteomic, metabolom...

journal_title：Journal of bacteriology

authors： Bertrand RL

更新日期：2019-03-13 00:00:00

abstract：:A new Salmonella enterica serovar Typhimurium strain has been constructed to facilitate tightly regulated gene expression. Arabinose-inducible and glucose-repressible expression of a T7 RNA polymerase gene that has been integrated with an adjacent araC-P(BAD) control element into the bacterial chromosome allows dynami...

journal_title：Journal of bacteriology

authors： McKinney J,Guerrier-Takada C,Galán J,Altman S

更新日期：2002-11-01 00:00:00

abstract：:In response to molecular oxygen and/or fixed nitrogen, the product of the Klebsiella pneumoniae nitrogen fixation L (nifL) gene inhibits NifA-mediated transcriptional activation. Nitrogen regulation of NifL function occurs at two levels: transcription of the nifLA operon is regulated by the general Ntr system, and the...

journal_title：Journal of bacteriology

authors： He L,Soupene E,Kustu S

更新日期：1997-12-01 00:00:00

abstract：:The gram-positive bacterium Bacillus subtilis contains two minimal Tat translocases, TatAdCd and TatAyCy, which are each involved in the secretion of one or more specific protein substrates. We have investigated the subcellular localization of the TatA components by employing C-terminal green fluorescent protein (GFP)...

journal_title：Journal of bacteriology

authors： Ridder AN,de Jong EJ,Jongbloed JD,Kuipers OP

更新日期：2009-07-01 00:00:00

abstract：:Methicillin resistance in staphylococci is mediated by PBP2a, a penicillin binding protein with low affinity for beta-lactam antibiotics. The gene encoding PBP2a, mecA, is transcriptionally regulated in some clinical isolates by mecR1 and mecI, genes divergently transcribed from mecA that encode a signal transducer an...

journal_title：Journal of bacteriology

authors： Sharma VK,Hackbarth CJ,Dickinson TM,Archer GL

更新日期：1998-04-01 00:00:00

abstract：:The ultrastructure of seven Thiobacillus species was studied. The structure of their cell envelopes is similar, if not identical, to that found in other gram-negative bacteria. Obvious differences were noted in the middle layer of the cell envelope of the seven cultures. Polyhedral inclusion bodies were apparent in fo...

journal_title：Journal of bacteriology

authors： Shively JM,Decker GL,Greenawalt JW

更新日期：1970-02-01 00:00:00

abstract：:Sequencing the insertion sites of 8,865 Tn917 insertions in Enterococcus faecalis strain OG1RF identified a hot spot in the replication terminus region corresponding to 6% of the genome where 65% of the transposons had inserted. In E. faecalis, Tn917 preferentially inserted at a 29-bp consensus sequence centered on TA...

journal_title：Journal of bacteriology

authors： Garsin DA,Urbach J,Huguet-Tapia JC,Peters JE,Ausubel FM

更新日期：2004-11-01 00:00:00

abstract：:Haemophilus influenzae is a common gram-negative pathogen that initiates infection by colonizing the upper respiratory tract epithelium. In previous work, we reported the isolation of a locus involved in expression of short, thin surface fibrils by H. influenzae type b and presented evidence that surface fibrils promo...

journal_title：Journal of bacteriology

authors： St Geme JW 3rd,Cutter D,Barenkamp SJ

更新日期：1996-11-01 00:00:00

abstract：:Oxalurate, the gratuitous inducer of the allantoin degradative enzymes, was taken into the cell by an energy-dependent active transport system with an apparent Km of 1.2 mM. Efflux of previously accumulated oxalurate was rapid, with a half-life of about 2 min. The oxalurate uptake system appears to be both constitutiv...

journal_title：Journal of bacteriology

authors： Cooper TG,McKelvey J,Sumrada R

更新日期：1979-09-01 00:00:00

abstract：:The single polar flagellum of Pseudomonas aeruginosa plays an important role in the pathogenesis of infection by this organism. However, regulation of the assembly of this organelle has not been delineated. In analyzing the sequence available at the Pseudomonas genome database, an open reading frame (ORF), flanked by ...

journal_title：Journal of bacteriology

authors： Dasgupta N,Arora SK,Ramphal R

更新日期：2000-01-01 00:00:00

abstract：:The distribution of bacterial L-ornithine: alpha-ketoglutarate delta-aminotransferase (L-ornithine:2-oxo-acid aminotransferase [EC 2.6.1.13]) was investigated, and Bacillus sphaericus (IFO 3525) was found to have the highest activity of the enzyme, which was inducibly formed by addition of L-ornithine or L-arginine to...

journal_title：Journal of bacteriology

authors： Yasuda M,Tanizawa K,Misono H,Toyama S,Soda K

更新日期：1981-10-01 00:00:00

abstract：:The transglycosylation step of cell wall synthesis is a prime antibiotic target because it is essential and specific to bacteria. Two antibiotics, ramoplanin and moenomycin, target this step by binding to the substrate lipid II and the transglycosylase enzyme, respectively. Here, we compare the ramoplanin and moenomyc...

journal_title：Journal of bacteriology

authors： Salzberg LI,Luo Y,Hachmann AB,Mascher T,Helmann JD

更新日期：2011-10-01 00:00:00

abstract：:Escherichia coli produces linoleic acid in the late stationary phase. This was the case whether the cultures were grown aerobically or anaerobically on a supplemented glucose-salts medium. The linoleic acid was detected by thin-layer chromatography and was measured as the methyl ester by gas chromatography. The linole...

journal_title：Journal of bacteriology

authors： Rabinowitch HD,Sklan D,Chace DH,Stevens RD,Fridovich I

更新日期：1993-09-01 00:00:00

abstract：:A pattern of allosteric control for aromatic biosynthesis in cyanobacteria relies upon early-pathway regulation as the major control point for the entire branched pathway. In Synechococcus sp. strain PCC6301 (Anacystis nidulans), two enzymes which form precursors for L-phenylalanine biosynthesis are subject to control...

journal_title：Journal of bacteriology

authors： Hall GC,Flick MB,Jensen RA

更新日期：1983-01-01 00:00:00

abstract：:Temperate bacteriophages play an important role in the pathogenicity of Staphylococcus aureus, for instance, by mediating the horizontal gene transfer of virulence factors. Here we established a classification scheme for staphylococcal prophages of the major Siphoviridae family based on integrase gene polymorphism. Se...

journal_title：Journal of bacteriology

authors： Goerke C,Pantucek R,Holtfreter S,Schulte B,Zink M,Grumann D,Bröker BM,Doskar J,Wolz C

更新日期：2009-06-01 00:00:00

abstract：:Widely studied Salmonella enterica serovar Typhimurium strains ATCC 14028s and SL1344 harbor a cryptic ST64B prophage unable to produce infectious virions. We found that coculturing either strain with an isogenic sibling lacking the prophage leads to the appearance of active forms of the virus. Active phage originates...

journal_title：Journal of bacteriology

authors： Figueroa-Bossi N,Bossi L

更新日期：2004-06-01 00:00:00

abstract：UNLABELLED:The σE envelope stress response is an essential signal transduction pathway which detects and removes mistargeted outer membrane (OM) β-barrel proteins (OMPs) in the periplasm of Escherichia coli It relies on σE, an alternative sigma factor encoded by the rpoE gene. Here we report a novel mutation, a nucleot...

journal_title：Journal of bacteriology

authors： Konovalova A,Schwalm JA,Silhavy TJ

更新日期：2016-08-11 00:00:00

abstract：:A fusion between lacZ and ftsZ of Escherichia coli was constructed to obtain a beta-galactosidase-FtsZ fusion protein. This fusion protein was used to raise antibodies against cell division protein FtsZ. Six monoclonal antibodies were obtained, and they reacted with FtsZ from cytoplasm and membrane fractions. The epit...

journal_title：Journal of bacteriology

authors： Voskuil JL,Westerbeek CA,Wu C,Kolk AH,Nanninga N

更新日期：1994-04-01 00:00:00

abstract：:The A-subunit polypeptides of Shiga toxin, the Shiga-like toxins (SLTs), and the plant lectin ricin inactivate eucaryotic ribosomes by enzymatically depurinating 28S rRNA. Comparison of the amino acid sequences of the members of the Shiga toxin family and ricin revealed two regions of significant homology that lie wit...

journal_title：Journal of bacteriology

authors： Jackson MP,Deresiewicz RL,Calderwood SB

更新日期：1990-06-01 00:00:00

abstract：:The glycosyltransferase (GT) module of class A penicillin-binding proteins (PBPs) and monofunctional GTs (MGTs) belong to the GT51 family in the sequence-based classification of GTs. They both possess five conserved motifs and use lipid II precursor (undecaprenyl-pyrophosphate-N-acetylglucosaminyl-N-acetylmuramoyl- pe...

journal_title：Journal of bacteriology

authors： Terrak M,Nguyen-Distèche M

更新日期：2006-04-01 00:00:00

abstract：:The R-factor R1 is present in a low number of copies per genome (near unity, so-called stringent control of replication). The replication of R1 was studied in a density-shift experiment. One generation after the shift about 20% of the R1 copies had not replicated, whereas about 20% had replicated at least twice. The r...

journal_title：Journal of bacteriology

authors： Gustafsson P,Nordström K

更新日期：1975-08-01 00:00:00

abstract：:Nearly every known species of Eubacteria encodes a homolog of the Borrelia burgdorferi EbfC DNA-binding protein. We now demonstrate that fluorescently tagged EbfC associates with B. burgdorferi nucleoids in vivo and that chromatin immunoprecipitation (ChIP) of wild-type EbfC showed it to bind in vivo to sites througho...

journal_title：Journal of bacteriology

authors： Jutras BL,Bowman A,Brissette CA,Adams CA,Verma A,Chenail AM,Stevenson B

更新日期：2012-07-01 00:00:00

abstract：:Attaching and effacing (AE) pathogens colonize the gut mucosa using a type three secretion system (T3SS) and a suite of effector proteins. The locus of enterocyte effacement (LEE) is the defining genetic feature of the AE pathogens, encoding the T3SS and the core effector proteins necessary for pathogenesis. Extensive...

journal_title：Journal of bacteriology

authors： Furniss RCD,Clements A

更新日期：2017-12-20 00:00:00

abstract：:Microarray-based comparisons of three Mycobacterium avium subsp. paratuberculosis isolates, including one sheep strain and two cattle strains, identified three large genomic deletions in the sheep strain, totaling 29,208 bp and involving 24 open reading frames. These deletions may help explain some of the differences ...

journal_title：Journal of bacteriology

authors： Marsh IB,Bannantine JP,Paustian ML,Tizard ML,Kapur V,Whittington RJ

更新日期：2006-03-01 00:00:00

abstract：:The method of shuttle mutagenesis has been extended to Neisseria gonorrhoeae. We have constructed a defective mini-Tn3 derivative that encodes chloramphenicol resistance in both N. gonorrhoeae and Escherichia coli and selected for mutations in the chloramphenicol resistance gene that express higher levels of antibioti...

journal_title：Journal of bacteriology

authors： Seifert HS,Ajioka RS,Paruchuri D,Heffron F,So M

更新日期：1990-01-01 00:00:00