Abstract:
:Sequencing the insertion sites of 8,865 Tn917 insertions in Enterococcus faecalis strain OG1RF identified a hot spot in the replication terminus region corresponding to 6% of the genome where 65% of the transposons had inserted. In E. faecalis, Tn917 preferentially inserted at a 29-bp consensus sequence centered on TATAA, a 5-bp sequence that is duplicated during insertion. The regional insertion site preference at the chromosome terminus was not observed in another low-G+C gram-positive bacterium, Listeria monocytogenes, although the consensus insertion sequence was the same. The 8,865 Tn917 insertion sites sequenced in E. faecalis corresponded to only approximately 610 different open reading frames, far fewer than the predicted number of 2,400, assuming random insertion. There was no significant preference in orientation of the Tn917 insertions with either transcription or replication. Even though OG1RF has a smaller genome than strain V583 (2.8 Mb versus 3.2 Mb), the only E. faecalis strain whose sequence is in the public domain, over 10% of the Tn917 insertions appear to be in a OG1RF-specific sequence, suggesting that there are significant genomic differences among E. faecalis strains.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Garsin DA,Urbach J,Huguet-Tapia JC,Peters JE,Ausubel FMdoi
10.1128/JB.186.21.7280-7289.2004keywords:
subject
Has Abstractpub_date
2004-11-01 00:00:00pages
7280-9issue
21eissn
0021-9193issn
1098-5530pii
186/21/7280journal_volume
186pub_type
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journal_title:Journal of bacteriology
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pub_type: 杂志文章
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更新日期:1978-12-01 00:00:00
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更新日期:1980-12-01 00:00:00
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更新日期:1985-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1982-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1988-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:1990-05-01 00:00:00