Cloning and characterization of the Salmonella typhimurium metE gene.

abstract：:The kinetic parameters of the release of Ca(2+)-dipicolinic acid (CaDPA) during germination of spore populations and multiple individual spores of Bacillus subtilis strains with major alterations in the structure of the spore peptidoglycan (PG) cortex or lacking one or both of the two redundant enzymes involved in cor...

journal_title：Journal of bacteriology

authors： Zhang P,Thomas S,Li YQ,Setlow P

更新日期：2012-02-01 00:00:00

abstract：:In Escherichia coli, rotation of the flagellar motor has been shown to depend upon electrostatic interactions between charged residues of the stator protein MotA and the rotor protein FliG. These charged residues are conserved in the Na+-driven polar flagellum of Vibrio alginolyticus, but mutational studies in V. algi...

journal_title：Journal of bacteriology

authors： Yakushi T,Yang J,Fukuoka H,Homma M,Blair DF

更新日期：2006-02-01 00:00:00

abstract：:Membrane vesicles isolated from competent cultures of Bacillus subtilis 168 bound up to 20 mug of double-stranded deoxyribonucleic acid (DNA) per mg of membrane protein in the presence of ethylenediaminetetraacetate. The formation of the DNA-membrane complex was time, temperature, and pH dependent. Eighty per cent of ...

journal_title：Journal of bacteriology

authors： Joenje H,Konings WN,Venema G

更新日期：1974-09-01 00:00:00

abstract：:The alpha (62,000-dalton) and beta (49,000-dalton) subunits of Methanosarcina barkeri ATPase were purified to homogeneity. The subunits and ATPase complex were trypsinized in the presence of various nucleotides. ATP and ADP changed the trypsin sensitivity of the alpha subunit in the complex and isolated forms, suggest...

journal_title：Journal of bacteriology

authors： Inatomi K,Maeda M

更新日期：1988-12-01 00:00:00

abstract：:Exposure of Escherichia coli strains deficient in molybdopterin biosynthesis (moa) to the purine base N-6-hydroxylaminopurine (HAP) is mutagenic and toxic. We show that moa mutants exposed to HAP also exhibit elevated mutagenesis, a hyperrecombination phenotype, and increased SOS induction. The E. coli rdgB gene encod...

journal_title：Journal of bacteriology

authors： Burgis NE,Brucker JJ,Cunningham RP

更新日期：2003-05-01 00:00:00

abstract：:Agrobacterium rhizogenes strain 15834, which incites hairy root disease in plants, harbors three large plasmids: pAr15834a (107 x 10(6) daltons), pAr15834b (154 x 10(6) daltons), and pAr15834c (258 x 10(6) daltons). Kanamycin-resistant transconjugants were selected in a cross of kanamycin-resistant derivate of strain ...

journal_title：Journal of bacteriology

authors： White FF,Nester EW

更新日期：1980-03-01 00:00:00

abstract：:At early stages of sporulation, the spoIIA locus is transcribed as a tricistronic (1.7-kb) operon, coding for sigma F and for two proteins that modulate the activity of sigma F. The locus is transcribed as a longer (2.9-kb) transcript at the late stages of sporulation. We show here that the longer transcript contains ...

journal_title：Journal of bacteriology

authors： Wu JJ,Schuch R,Piggot PJ

更新日期：1992-08-01 00:00:00

abstract：:The first common enzyme of isoleucine and valine biosynthesis, acetolactate synthase (ALS), is specifically inhibited by the herbicide sulfometuron methyl (SM). To further understand the physiological consequences of flux alterations at this point in metabolism, Escherichia coli genes whose expression was induced by p...

journal_title：Journal of bacteriology

authors： Van Dyk TK,Ayers BL,Morgan RW,Larossa RA

更新日期：1998-02-01 00:00:00

abstract：:Caulobacter crescentus cells adhere to surfaces by using an extremely strong polar adhesin called the holdfast. The polysaccharide component of the holdfast is comprised in part of oligomers of N-acetylglucosamine. The genes involved in the export of the holdfast polysaccharide and the anchoring of the holdfast to the...

journal_title：Journal of bacteriology

authors： Toh E,Kurtz HD Jr,Brun YV

更新日期：2008-11-01 00:00:00

abstract：:The serotype 5 capsule gene cluster of Staphylococcus aureus comprises 16 genes (cap5A through cap5P), but little is known about how the putative gene products function in capsule biosynthesis. We propose that the N-acetylmannosaminuronic acid (ManNAcA) component of the S. aureus serotype 5 capsular polysaccharide (CP...

journal_title：Journal of bacteriology

authors： Kiser KB,Bhasin N,Deng L,Lee JC

更新日期：1999-08-01 00:00:00

abstract：:The formation of recombinants in Hfr crosses was studied in Escherichia coli strains carrying combinations of genes known to affect recombination and DNA repair. Mutations in ruv and recG eliminate activities that have been shown to process Holliday junction intermediates by nuclease cleavage and/or branch migration. ...

journal_title：Journal of bacteriology

authors： Ryder L,Whitby MC,Lloyd RG

更新日期：1994-03-01 00:00:00

abstract：:The two-partner secretion system ExlBA, expressed by strains of Pseudomonas aeruginosa belonging to the PA7 group, induces hemorrhage in lungs due to disruption of host cellular membranes. Here we demonstrate that the exlBA genes are controlled by a pathway consisting of cAMP and the virulence factor regulator (Vfr). ...

journal_title：Journal of bacteriology

authors： Berry A,Han K,Trouillon J,Robert-Genthon M,Ragno M,Lory S,Attrée I,Elsen S

更新日期：2018-05-24 00:00:00

abstract：:Nosocomial infections caused by antibiotic-resistant Klebsiella pneumoniae are emerging as a major health problem worldwide, while community-acquired K. pneumoniae infections present with a range of diverse clinical pictures in different geographic areas. In particular, an invasive form of K. pneumoniae that causes li...

journal_title：Journal of bacteriology

authors： Wu KM,Li LH,Yan JJ,Tsao N,Liao TL,Tsai HC,Fung CP,Chen HJ,Liu YM,Wang JT,Fang CT,Chang SC,Shu HY,Liu TT,Chen YT,Shiau YR,Lauderdale TL,Su IJ,Kirby R,Tsai SF

更新日期：2009-07-01 00:00:00

abstract：:Klebsiella pneumoniae porin genes were analyzed to detect mutations accounting for the porin deficiency observed in many beta-lactam-resistant strains. PCR and Southern blot analysis revealed the existence of a third porin gene in addition to the OmpK36 and OmpK35 porin genes previously described. This new porin gene ...

journal_title：Journal of bacteriology

authors： Doménech-Sánchez A,Hernández-Allés S,Martínez-Martínez L,Benedí VJ,Albertí S

更新日期：1999-05-01 00:00:00

abstract：:Nickel-deficient (Nic-) mutants of Alcaligenes eutrophus requiring high levels of nickel ions for autotrophic growth with hydrogen were characterized. The Nic- mutants carried defined deletions in the hydrogenase gene cluster of the indigenous pHG megaplasmid. Nickel deficiency correlated with a low level of the nicke...

journal_title：Journal of bacteriology

authors： Eberz G,Eitinger T,Friedrich B

更新日期：1989-03-01 00:00:00

abstract：:Expression of fimA, the structural gene for type 1 fimbriae of Escherichia coli, is phase variable. Significant homologies were identified between the recombinases which control fimbrial phase variation, FimB and FimE, and the integrase class of site-specific recombinases. Normal expression of fimA was shown to requir...

journal_title：Journal of bacteriology

authors： Dorman CJ,Higgins CF

更新日期：1987-08-01 00:00:00

abstract：:Cystic fibrosis (CF) patients are highly susceptible to chronic pulmonary disease caused by mucoid Pseudomonas aeruginosa strains that overproduce the exopolysaccharide alginate. We showed here that a mutation in zwf, encoding glucose-6-phosphate dehydrogenase (G6PDH), leads to a approximately 90% reduction in alginat...

journal_title：Journal of bacteriology

authors： Silo-Suh L,Suh SJ,Phibbs PV,Ohman DE

更新日期：2005-11-01 00:00:00

abstract：:Burkholderia thailandensis is a soil saprophyte that is closely related to the pathogen Burkholderia pseudomallei, the etiological agent of melioidosis in humans. The environmental niches and infection sites occupied by these bacteria are thought to contain only limited concentrations of oxygen, where they can generat...

journal_title：Journal of bacteriology

authors： Vitale A,Paszti S,Takahashi K,Toyofuku M,Pessi G,Eberl L

更新日期：2020-11-04 00:00:00

abstract：:A monoclonal antibody (MAb), MAb 900, which detects a 43-kDa protein present on Escherichia coli was found. Subsequently, more than 90 organisms, belonging to either the bacterial, archaeal, or eucaryal domain, were tested for reactivity to this MAb. Of the bacterial and archaeal domains, almost all species proved to ...

journal_title：Journal of bacteriology

authors： Weber S,Lottspeich F,Köhl J

更新日期：1995-01-01 00:00:00

abstract：:Genes involved in the biosynthesis of leucine have been mapped in Bacillus megaterium QM B1551, using transducing phage MP13. Mutations were designated leuA, leuB, or leuC on the basis of enzyme assays. Two mutant strains were deficient in the enzyme activities of leuA (alpha-isopropylmalate synthase) and leuC (beta-i...

journal_title：Journal of bacteriology

authors： Garbe JC,Hess GF,Franzen MA,Vary PS

更新日期：1984-02-01 00:00:00

abstract：:The genes for the complete pathways for two polycyclic aromatic polyketides of the angucyclinone class have been cloned and heterologously expressed. Genomic DNAs of Streptomyces rimosus NRRL 3016 and Streptomyces strain WP 4669 were partially digested with MboI, and libraries (ca. 40-kb fragments) in Escherichia coli...

journal_title：Journal of bacteriology

authors： Hong ST,Carney JR,Gould SJ

更新日期：1997-01-01 00:00:00

abstract：:We have detected a cholesterol-dependent cytolysin, which we have named mitilysin, in a small number of Streptococcus mitis isolates. We have sequenced the mitilysin gene from seven isolates of S. mitis. Comparisons with the pneumococcal pneumolysin gene show 15 amino acid substitutions. S. mitis appear to release mit...

journal_title：Journal of bacteriology

authors： Jefferies J,Nieminen L,Kirkham LA,Johnston C,Smith A,Mitchell TJ

更新日期：2007-01-01 00:00:00

abstract：:An 8,883-bp mini-pXO1 plasmid containing a replicon from Bacillus anthracis pXO1 (181.6 kb) was identified by making large deletions in the original plasmid using a newly developed Cre-loxP system. Portions of the truncated mini-pXO1 were cloned into an Escherichia coli vector unable to replicate in B. anthracis. A 5....

journal_title：Journal of bacteriology

authors： Pomerantsev AP,Camp A,Leppla SH

更新日期：2009-08-01 00:00:00

abstract：:Garrett et al. (Mol. Gen. Genet. 182:326-331, 1981) constructed strains of Escherichia coli harboring derivatives of plasmid pBR322 that carry the lysis genes (S, R, and Rz) of phage lambda. The plasmid construction placed the genes under control of the lactose operon operator-promotor (and thus of lac repressor). Ind...

journal_title：Journal of bacteriology

authors： Crabtree S,Cronan JE Jr

更新日期：1984-04-01 00:00:00

abstract：UNLABELLED:Misincorporation of D-tyrosine (D-Tyr) into cellular proteins due to mischarging of tRNA(Tyr) with D-Tyr by tyrosyl-tRNA synthetase inhibits growth and biofilm formation of Bacillus subtilis. Furthermore, many B. subtilis strains lack a functional gene encoding D-aminoacyl-tRNA deacylase, which prevents misi...

journal_title：Journal of bacteriology

authors： Williams-Wagner RN,Grundy FJ,Raina M,Ibba M,Henkin TM

更新日期：2015-05-01 00:00:00

abstract：:Hoffman, Heiner (New York University, New York, N.Y.), Jon Valdina, and Michael E. Frank. Effects of high incubation temperature upon the cell wall of Escherichia coli. J. Bacteriol. 91:1635-1637. 1966.-Escherichia coli cells grown at 45 C were found to swell when suspended in distilled water, and were broken down whe...

journal_title：Journal of bacteriology

authors： Hoffman H,Valdina J,Frank ME

更新日期：1966-04-01 00:00:00

abstract：:The fis operon from Salmonella typhimurium has been cloned and sequenced, and the properties of Fis-deficient and Fis-constitutive strains were examined. The overall fis operon organization in S. typhimurium is the same as that in Escherichia coli, with the deduced Fis amino acid sequences being identical between both...

journal_title：Journal of bacteriology

authors： Osuna R,Lienau D,Hughes KT,Johnson RC

更新日期：1995-04-01 00:00:00

abstract：:The ability to restart broken DNA replication forks is essential across all domains of life. In Escherichia coli, the priA, priB, priC, and dnaT genes encode the replication restart proteins (RRPs) to accomplish this task. PriA plays a critical role in replication restart such that its absence reveals a dramatic pheno...

journal_title：Journal of bacteriology

authors： Leroux M,Jani N,Sandler SJ

更新日期：2017-08-08 00:00:00

abstract：:Motility in mycobacteria was described for the first time in 1999. It was reported that Mycobacterium smegmatis and Mycobacterium avium could spread on the surface of solid growth medium by a sliding mechanism and that the presence of cell wall glycopeptidolipids was essential for motility. We recently reported that M...

journal_title：Journal of bacteriology

authors： Agustí G,Astola O,Rodríguez-Güell E,Julián E,Luquin M

更新日期：2008-10-01 00:00:00

abstract：:The bacterium Rhodobacter capsulatus is capable of an unusual form of genetic exchange, mediated by a transducing bacteriophage-like particle called the gene transfer agent (GTA). GTA production by R. capsulatus is controlled at the level of transcription by a cellular two-component signal transduction system that inc...

journal_title：Journal of bacteriology

authors： Lang AS,Beatty JT

更新日期：2002-02-01 00:00:00