Identification of a secreted cholesterol-dependent cytolysin (mitilysin) from Streptococcus mitis.

abstract：:The N-terminal amino acid sequence of the polyhydroxyalkanoic acid (PHA) granule-associated M(r)-15,500 protein of Rhodococcus ruber (the GA14 protein) was analyzed. The sequence revealed that the corresponding structural gene is represented by open reading frame 3, encoding a protein with a calculated M(r) of 14,175 ...

journal_title：Journal of bacteriology

authors： Pieper-Fürst U,Madkour MH,Mayer F,Steinbüchel A

更新日期：1994-07-01 00:00:00

abstract：:The electron transport system of Acinetobacter sp. HO1-N was studied to determine the specific cytochromes and to measure changes in the composition of the respiratory system due to growth in various concentrations of oxygen or types of growth substrates. Spectrophotometric analysis revealed that the quantity and type...

journal_title：Journal of bacteriology

authors： Ensley BD Jr,Finnerty WR

更新日期：1980-06-01 00:00:00

abstract：:Despite their remarkable genetic homology, members of the Mycobacterium tuberculosis complex express very different phenotypes, most notably in their spectra of clinical presentation. For example, M. tuberculosis is regarded as pathogenic to humans, whereas members having deleted RD1, such as Mycobacterium microti and...

journal_title：Journal of bacteriology

authors： Mostowy S,Cousins D,Behr MA

更新日期：2004-01-01 00:00:00

abstract：:Oligopeptidase B (OpdB) is a serine peptidase broadly distributed among unicellular eukaryotes, gram-negative bacteria, and spirochetes which has emerged as an important virulence factor and potential therapeutic target in infectious diseases. We report here the cloning and expression of the opdB homologue from Salmon...

journal_title：Journal of bacteriology

authors： Morty RE,Fülöp V,Andrews NW

更新日期：2002-06-01 00:00:00

abstract：:Utilization of heme as an iron source by Bradyrhizobium japonicum involves induction of the outer membrane heme receptor gene hmuR and other genes within the heme utilization locus. Here, we discovered the hmuP gene located upstream of hmuR and transcribed divergently from it along with hmuTUV. hmuP encodes a small pr...

journal_title：Journal of bacteriology

authors： Escamilla-Hernandez R,O'Brian MR

更新日期：2012-06-01 00:00:00

abstract：:Most Pseudomonas aeruginosa strains are able to produce two distinct lipopolysaccharide (LPS) O-polysaccharide types, A-band (common-antigen) and B-band (serotype-specific) LPSs. The relative expression levels of these two LPS types in P. aeruginosa PAO1 (O5 serotype) at various growth temperatures were investigated b...

journal_title：Journal of bacteriology

authors： Makin SA,Beveridge TJ

更新日期：1996-06-01 00:00:00

abstract：:The Yersinia pestis proteome was studied as a function of temperature and calcium by two-dimensional differential gel electrophoresis. Over 4,100 individual protein spots were detected, of which hundreds were differentially expressed. A total of 43 differentially expressed protein spots, representing 24 unique protein...

journal_title：Journal of bacteriology

authors： Chromy BA,Choi MW,Murphy GA,Gonzales AD,Corzett CH,Chang BC,Fitch JP,McCutchen-Maloney SL

更新日期：2005-12-01 00:00:00

abstract：:Cystic fibrosis transmembrane conductance regulator (CFTR) inhibitory factor (Cif) is a virulence factor secreted by Pseudomonas aeruginosa that reduces the quantity of CFTR in the apical membrane of human airway epithelial cells. Initial sequence analysis suggested that Cif is an epoxide hydrolase (EH), but its seque...

journal_title：Journal of bacteriology

authors： Bahl CD,Morisseau C,Bomberger JM,Stanton BA,Hammock BD,O'Toole GA,Madden DR

更新日期：2010-04-01 00:00:00

abstract：:The folded chromosome of Escherichia coli has been investigated under various lysis and physiological conditions. A new gradient system was devised that allows excellent separation between unlysed cells and envelope-associated and envelope-free chromosomes. Isotope incorporation experiments showed that the fraction of...

journal_title：Journal of bacteriology

authors： Korch C,Ovrebo S,Kleppe K

更新日期：1976-08-01 00:00:00

abstract：:In a glycerol auxotroph of Staphylococcus aureus, the deprivation of glycerol affected the formation of certain membrane components. (i) There was synthesis of fatty acids at the predeprivation rate even though the fatty acids synthesized accumulated as free fatty acids rather than as esterified fatty acids; (ii) ther...

journal_title：Journal of bacteriology

authors： Ray PH,Lillich TT,White DC

更新日期：1972-10-01 00:00:00

abstract：:Replication of the broad-host-range, IncPalpha plasmid RK2 requires two plasmid loci: trfA, the replication initiator gene, and oriV, the origin of replication. While these determinants are sufficient for replication in a wide variety of bacteria, they do not confer the stable maintenance of parental RK2 observed in i...

journal_title：Journal of bacteriology

authors： Rosche TM,Siddique A,Larsen MH,Figurski DH

更新日期：2000-11-01 00:00:00

abstract：:Pseudomonas MS can grow on methylamine and a number of other compounds containing C1 units as a sole source of carbon and energy. Assimilation of carbon into cell material occurs via the "serine pathway" since enzymes of this pathway are induced after growth on methylamine, but not malate or acetate. A mutant has been...

journal_title：Journal of bacteriology

authors： Wagner C,Levitch ME

更新日期：1975-06-01 00:00:00

abstract：:HypA and HypB are maturation proteins required for incorporation of nickel into the hydrogenase large subunit. To examine the functions of these proteins in nickel insertion, the hybF gene, which is a homolog of hypA essential for maturation of hydrogenases 1 and 2 from Escherichia coli, was overexpressed, and the pro...

journal_title：Journal of bacteriology

authors： Blokesch M,Rohrmoser M,Rode S,Böck A

更新日期：2004-05-01 00:00:00

abstract：:PacI and SpeI restriction maps were obtained for the two chromosomes of each of the six species of the genus Brucella: B. melitensis, B. abortus, B. suis, B. canis, B. ovis, and B. neotomae. Three complementary techniques were used: hybridization with the two replicons as probes, cross-hybridization of restriction fra...

journal_title：Journal of bacteriology

authors： Michaux-Charachon S,Bourg G,Jumas-Bilak E,Guigue-Talet P,Allardet-Servent A,O'Callaghan D,Ramuz M

更新日期：1997-05-01 00:00:00

abstract：:We previously clarified that the chitinase from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 produces diacetylchitobiose (GlcNAc(2)) as an end product from chitin. Here we sought to identify enzymes in T. kodakaraensis that were involved in the further degradation of GlcNAc(2). Through a search of th...

journal_title：Journal of bacteriology

authors： Tanaka T,Fukui T,Atomi H,Imanaka T

更新日期：2003-09-01 00:00:00

abstract：:Quantitative mass conversion of intact bacterial cells of Bacillus subtilis and B. licheniformis to L-phase variants has been effected after lysozyme treatment. After subculture of the unstable L-phase variants for several months in the presence of methicillin, stable L-phase variants were obtained which grew in the a...

journal_title：Journal of bacteriology

authors： Wyrick PB,Rogers HJ

更新日期：1973-10-01 00:00:00

abstract：:Linker insertion mutagenesis was used to modify the paracrystalline surface layer (S-layer) protein (RsaA) of the gram-negative bacterium Caulobacter crescentus. Eleven unique BamHI linker insertions in the cloned rsaA gene were identified; at the protein level, these linker insertions introduced 4 to 6 amino acids at...

journal_title：Journal of bacteriology

authors： Bingle WH,Nomellini JF,Smit J

更新日期：1997-02-01 00:00:00

abstract：:Seven mutants of Haemophilus influenzae were isolated by the criterion of sensitivity to ultraviolet (UV) inactivation of colony formation. These mutants and the wild type were characterized with regard to X-ray inactivation of colony formation, UV induction of division inhibition, the ability of the eight strains to ...

journal_title：Journal of bacteriology

authors： Setlow JK,Brown DC,Boling ME,Mattingly A,Gordon MP

更新日期：1968-02-01 00:00:00

abstract：:The composition of the molecular species of acidic phospholipids in Escherichia coli B during the late exponential growth phase at 37 degrees C was determined. Two phosphatidyl groups of cardiolipin, the 3-(3-sn-phosphatidyl) and 1-(3-sn-phosphatidyl) moieties of cardiolipin, were isolated by limited hydrolysis with p...

journal_title：Journal of bacteriology

authors： Yokota K,Kanamoto R,Kito M

更新日期：1980-03-01 00:00:00

abstract：:On the basis of information available in the literature, gluconate dissimilation in Escherichia coli is thought to occur via the hexose monophosphate pathway. Evidence is presented in this study that gluconate is catabolized in this organism via an inducible Entner-Doudoroff pathway. This evidence is based on chromato...

journal_title：Journal of bacteriology

authors： Eisenberg RC,Dobrogosz WJ

更新日期：1967-03-01 00:00:00

abstract：:A highly efficient protoplast transformation system for Streptococcus faecalis has been developed by systematically optimizing different parameters. Up to 10(6) transformants per micrograms of DNA were consistently obtained within 3 days, and cell wall regeneration of protoplasts was virtually 100%. A systematic searc...

journal_title：Journal of bacteriology

authors： Wirth R,An FY,Clewell DB

更新日期：1986-03-01 00:00:00

abstract：:Genetic studies have identified a specificity domain for prohead binding in the C-terminal 32 amino acids of gpA, the large subunit of bacteriophage lambda terminase (S. Frackman, D. A. Siegele, and M. Feiss, J. Mol. Biol. 180:283-300, 1984). In the present work, an amber mutation, Aam42, in the fifth-to-last codon of...

journal_title：Journal of bacteriology

authors： Sippy J,Feiss M

更新日期：1992-02-01 00:00:00

abstract：:Fructose, galactose, L-arabinose, gluconate, and several organic acids support rapid growth and N2 fixation of Azospirillum brasiliense ATCC 29145 (strain Sp7) as a sole source of carbon and energy. Growth of Azospirillum lipoferum ATCC 29707 (strain Sp59b) is also supported by glucose, mannose, mannitol, and alpha-ke...

journal_title：Journal of bacteriology

authors： Martinez-Drets G,Del Gallo M,Burpee C,Burris RH

更新日期：1984-07-01 00:00:00

abstract：:A sulfonic acid found to be a major constituent of spores of Bacillus subtilis was provisionally identified as 3-l-sulfolactic acid. This compound was completely absent from vegetative cells during growth, but large amounts accumulated in sporulating cells just before the development of refractile spores. Essentially ...

journal_title：Journal of bacteriology

authors： Bonsen PP,Spudich JA,Nelson DL,Kornberg A

更新日期：1969-04-01 00:00:00

abstract：:The YycFG two-component system is the only signal transduction system in Bacillus subtilis known to be essential for cell viability. This system is highly conserved in low-G+C gram-positive bacteria, regulating important processes such as cell wall homeostasis, cell membrane integrity, and cell division. Four other ge...

journal_title：Journal of bacteriology

authors： Szurmant H,Mohan MA,Imus PM,Hoch JA

更新日期：2007-04-01 00:00:00

abstract：:MotA contains a conserved C-terminal cluster of negatively charged residues, and MotB contains a conserved N-terminal cluster of positively charged residues. Charge-altering mutations affecting these residues impair motility but do not diminish Mot protein levels. The motility defects are reversed by second-site mutat...

journal_title：Journal of bacteriology

authors： Hosking ER,Manson MD

更新日期：2008-08-01 00:00:00

abstract：:Forty-nine human and environmental isolates of Mycobacterium intracellulare and Mycobacterium scrofulaceum were tested for their ability to grow on uric acid and a number of its degradation products. Nearly all (88 to 90%) strains used uric acid or allantoin as a sole nitrogen source; fewer (47 to 69%) used allantoate...

journal_title：Journal of bacteriology

authors： Falkinham JO 3rd,George KL,Parker BC,Gruft H

更新日期：1983-07-01 00:00:00

abstract：:McFadden, B. A. (Washington State University, Pullman), and H. R. Homann. Characteristics and intermediates of short-term C(14)O(2) incorporation during ribose oxidation by Hydrogenomonas facilis. J. Bacteriol. 89:839-847. 1965.-Ribose-grown cells of Hydrogenomonas facilis, which had been suspended in growth medium an...

journal_title：Journal of bacteriology

authors： MCFADDEN BA,HOMANN HR

更新日期：1965-03-01 00:00:00

abstract：:An electron microscopy study has been made of the effects of dissolution of the plasma membrane of Escherichia coli with sodium dodecyl sulfate (SDS) on the organization of the nucleoplasm and the cytoplasm. The alterations observed in time course experiments were related to absorbance changes and to release of macrom...

journal_title：Journal of bacteriology

authors： Woldringh CL,van Iterson W

更新日期：1972-09-01 00:00:00

abstract：:The Shiga toxin operon (stx) is composed of two genes for the A and B subunits, which are transcribed from a promoter 5' to the stxA gene. The 1A:5B subunit stoichiometry of the holotoxin suggests that the stxA and stxB genes are differentially regulated. In a previous study, we demonstrated the existence of a second ...

journal_title：Journal of bacteriology

authors： Habib NF,Jackson MP

更新日期：1993-02-01 00:00:00