Abstract:
:An electron microscopy study has been made of the effects of dissolution of the plasma membrane of Escherichia coli with sodium dodecyl sulfate (SDS) on the organization of the nucleoplasm and the cytoplasm. The alterations observed in time course experiments were related to absorbance changes and to release of macromolecules from the cells. As the cells became plasmolyzed, under the conditions used, the first visible effect of SDS was a collapse of the plasmolysis spaces. This was accompanied by a displacement of the nuclear material which then appeared in broad contact with the redeployed plasma membrane. This initial displacement of nuclear material to the cell border may indicate an association between the nucleoplasm and the plasma membrane. Upon further dissolution of the plasma membrane, the nuclear material receded from the cell margin and contracted into an axial filament. Meanwhile, the cytoplasm dissociated into an amorphous, Pronase-sensitive component and an electron-opaque, granular one sensitive to ribonuclease. The latter represented one continuous area of ribosomal structures surrounding the nucleoplasm, an organization which did not occur when the cells were inhibited with rifamycin before SDS treatment. During prolonged SDS interaction, approximately 65% of the cellular protein, 25% of the ribonucleic acid and 40% of the deoxyribonucleic acid were released from the cells concomitant with the disappearance of the amorphous cytoplasmic part, expansion of the ribosomal aggregate, and rearrangement of the nuclear material at the cell periphery. The observations support the contention that all ribosomal structures bear a direct relationship with the nucleoplasm.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Woldringh CL,van Iterson Wdoi
10.1128/JB.111.3.801-813.1972subject
Has Abstractpub_date
1972-09-01 00:00:00pages
801-13issue
3eissn
0021-9193issn
1098-5530journal_volume
111pub_type
杂志文章abstract::The process of flagellar assembly in Salmonella typhimurium was investigated by using temperature-sensitive mutants. The mutants were grown at the restrictive temperature and then at the permissive temperature, with radiolabel supplied in the first phase of the experiment and not the second, or vice versa. Flagellar h...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.3.1327-1339.1990
更新日期:1990-03-01 00:00:00
abstract::Nakata, H. M. (Washington State University, Pullman). Effect of pH on intermediates produced during growth and sporulation of Bacillus cereus. J. Bacteriol. 86:577-581. 1963.-Cultures of Bacillus cereus strain T, grown in an unbuffered glucose-yeast extract-mineral salts medium and in the same medium buffered at pH 6....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.86.3.577-581.1963
更新日期:1963-09-01 00:00:00
abstract::Addition of casein hydrolysate to suspensions of washed, nonpigmented, nonproliferating Serratia marcescens incubating at 27 C induced biosynthesis of prodigiosin. Four amino acids of casein hydrolysate, dl-aspartic acid, l-glutamic acid, l-proline, and l-alanine caused formation of pigment when added individually. dl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.106.2.444-448.1971
更新日期:1971-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01354-06
更新日期:2007-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.7.2196-2205.1991
更新日期:1991-04-01 00:00:00
abstract::A novel regulatory gene, trh, which is involved in hrp gene expression, is identified in the plant pathogen Xanthomonas oryzae pv. oryzae. In the trh mutant, expression of HrpG, which is a key regulator for hrp gene expression, is reduced both under the in vitro hrp-inducing condition and in planta. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00006-06
更新日期:2006-06-01 00:00:00
abstract::SaPIbov2 is a member of the SaPI family of staphylococcal pathogenicity islands and is very closely related to SaPIbov1. Typically, certain temperate phages can induce excision and replication of one or more of these islands and can package them into special small phage-like particles commensurate with their genome si...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00619-07
更新日期:2007-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.2.689-694.1999
更新日期:1999-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00340-16
更新日期:2016-08-11 00:00:00
abstract::For the pathogenic yeast Candida albicans, secreted aspartyl proteinase (Sap) activity has been correlated with virulence. A family consisting of at least eight SAP genes can be drawn upon to produce Sap enzymatic activity. In this study, the levels of Sap1, Sap2, and Sap3 isoenzymes were monitored under a variety of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.18.5215-5221.1995
更新日期:1995-09-01 00:00:00
abstract::A nonswarming Serratia liquefaciens mutant deficient in serrawettin W2 production was constructed by transposon mutagenesis. Sequence homology indicated that insertion had occurred in gene swrA, which encodes a putative peptide synthetase. Expression of swrA is controlled by quorum sensing. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:
更新日期:1998-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.16.5140-5144.1994
更新日期:1994-08-01 00:00:00
abstract::S. Sawada and co-workers reported that a monoclonal antibody (MAb), E87, interacted with about 80% of Pseudomonas aeruginosa isolates, and they separated a rhamnose-rich polysaccharide as the probable antigen for MAb E87 from P. aeruginosa IFO 3080 (S. Sawada, T. Kawamura, Y. Masuho, and K. Tomibe, J. Infec. Dis. 152:...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.10.6162-6164.1990
更新日期:1990-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.23.6736-6742.1996
更新日期:1996-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.4.1277-1283.2001
更新日期:2001-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.140.2.738-741.1979
更新日期:1979-11-01 00:00:00
abstract::Unlike the great majority of the aerobactin-producing enteric bacteria documented in the literature, Enterobacter cloacae EK33, isolated from a case of human neonatal meningitis, did not show any homology at the DNA level with the prototype aerobactin system encoded by the ColV-K30 plasmid. However, both the nuclear m...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.12.5539-5544.1988
更新日期:1988-12-01 00:00:00
abstract::An examination of the methanofurans isolated from a wide range of methanogenic bacteria and from Archaeoglobus fulgidus has revealed at least five chromatographically distinct methanofurans. Bacteria from each major genus of methanogenic bacteria have been found to contain a chemically different methanofuran. The natu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.10.4594-4597.1988
更新日期:1988-10-01 00:00:00
abstract::Resting cells of Corynebacterium nephridii reduce nitrate, nitrite, and nitric oxide to nitrous oxide under anaerobic conditions. Nitrous oxide production from nitrite was optimal from pH 7.0 to 7.4. The stoichiometry of nitrous oxide production from nitrite was 99% of the theoretical-two moles of nitrite was used for...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.101.3.821-826.1970
更新日期:1970-03-01 00:00:00
abstract::A detailed study of the cultural characteristics and cellular fatty acid composition of 27 isolates of Corynebacterium acnes was performed to establish the properties by which this organism may be identified and characterized. The fatty acids were extracted directly from whole cells and examined as methyl esters by ga...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.5.1300-1305.1967
更新日期:1967-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.17.4672-4680.2002
更新日期:2002-09-01 00:00:00
abstract::S-Adenosyl-L-methionine:precorrin-2 methyltransferase (SP2MT), which catalyzes the C-20 methylation of precorrin-2 to precorrin-3, was purified to homogeneity from extracts of a recombinant strain of Pseudomonas denitrificans derived from a cobalamin-overproducing strain. Ammonium sulfate fractionation followed by chr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.11.6245-6251.1990
更新日期:1990-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.4.1719-1724.1990
更新日期:1990-04-01 00:00:00
abstract::Mycoplasmas isolated from simian (Cercopithecus aethiops) tissues were shown to have biological, biochemical, and serological properties and electrophoretic cell protein patterns similar to strain Navel isolated from man 15 years ago. The simian and human Navel strains comprised a single serogroup, distinct from the e...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.108.1.439-445.1971
更新日期:1971-10-01 00:00:00
abstract::Azotobacter vinelandii DJ71, which contains a mutation in the nifV gene, was derepressed for nitrogenase in the presence of homocitrate. When dinitrogenase was isolated from this culture, it was found to be identical to the wild-type dinitrogenase. However, when the same NifV- strain was derepressed in the presence of...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.17.5403-5405.1991
更新日期:1991-09-01 00:00:00
abstract::Holwerda, Jack (Michigan Department of Health, Grand Rapids) and Grace Eldering. Culture and fluorescent-antibody methods in diagnosis of whooping cough. J. Bacteriol. 86:449-451. 1963.-Nasopharyngeal swabs from 517 suspected whooping-cough patients were examined by culture and by fluorescent-antibody (FA) staining pr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.86.3.449-451.1963
更新日期:1963-09-01 00:00:00
abstract::Oligoribonuclease, an exoribonuclease specific for small oligoribonucleotides, was initially characterized 20 years ago (S. K. Niyogi and A. K. Datta, J. Biol. Chem. 250:7307-7312, 1975) and shown to be different from RNase II and polynucleotide phosphorylase. Here we demonstrate, using mutant strains and purified enz...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.14.4137-4139.1995
更新日期:1995-07-01 00:00:00
abstract::Successful completion of the bacteriophage P2 lytic cycle requires phage-induced lysis of its Escherichia coli host, a process that is poorly understood. Genetic analysis of lysis-deficient mutants defined a single locus, gene K, which lies within the largest late transcription unit of P2 and maps between head gene L ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.16.4974-4984.1994
更新日期:1994-08-01 00:00:00
abstract::The expression of the positively charged human protein secretory leukocyte protease inhibitor (SLPI) in Escherichia coli causes severe cellular toxicity. After induction of SLPI synthesis in a high-level-expression strain, SGE61, the growth of the strain is arrested and total protein and RNA synthesis rates decline by...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.4.2166-2172.1989
更新日期:1989-04-01 00:00:00
abstract::We used two-dimensional gel electrophoresis to quantitate the changes in rates of synthesis that follow phage lambda infection for 21 Escherichia coli proteins, including groE and dnaK proteins. Although total protein synthesis and the rates of synthesis of most individual E. coli proteins decreased after infection, s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.3.1050-1063.1982
更新日期:1982-03-01 00:00:00