Abstract:
:Genetic studies have identified a specificity domain for prohead binding in the C-terminal 32 amino acids of gpA, the large subunit of bacteriophage lambda terminase (S. Frackman, D. A. Siegele, and M. Feiss, J. Mol. Biol. 180:283-300, 1984). In the present work, an amber mutation, Aam42, in the fifth-to-last codon of the A gene was found to be lethal in nonsuppressing hosts. The mutation, expected to generate gpA lacking the last five amino acids, caused the production of a terminase that cut cos efficiently both in vivo and in vitro but was defective in DNA packaging. lambda Aam42 lysates contained unused proheads, consistent with a defect in prohead binding. Aam42 terminase was more strongly dependent than wild-type terminase on gpFI, the catalyst of prohead binding. Like wild-type terminase, Aam42 terminase did not cut cos in vivo when prohead assembly was blocked by a mutation in one of the genes encoding the prohead.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Sippy J,Feiss Mdoi
10.1128/jb.174.3.850-856.1992keywords:
subject
Has Abstractpub_date
1992-02-01 00:00:00pages
850-6issue
3eissn
0021-9193issn
1098-5530journal_volume
174pub_type
杂志文章abstract::The biochemical consequences of inositol limitation in an inositol auxotroph of Neurospora crassa have been examined as a means of disclosing the cellular role of inositol. The cellular levels of inositol in the inl mutant were proportional to the concentration of inositol in the growth medium whereas inositol phospha...
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更新日期:1997-02-01 00:00:00
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更新日期:1997-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1998-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1998-07-01 00:00:00
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更新日期:1984-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1969-03-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1988-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1985-08-01 00:00:00