Induction of the SOS response in Escherichia coli inhibits Tn5 and IS50 transposition.


:In response to DNA damage or the inhibition of normal DNA replication in Escherichia coli, a set of some 20 unlinked operons is induced through the RecA-mediated cleavage of the LexA repressor. We examined the effect of this SOS response on the transposition of Tn5 and determined that the frequency of transposition is reduced 5- to 10-fold in cells that constitutively express SOS functions, e.g., lexA(Def) strains. Furthermore, this inhibition is independent of recA function, is fully reversed by a wild-type copy of lexA, and is not caused by an alteration in the levels of the Tn5 transposase or inhibitor proteins. We isolated insertion mutations in a lexA(Def) background that reverse this transposition defect; all of these mapped to a new locus near 23 min on the E. coli chromosome.


J Bacteriol


Journal of bacteriology


Weinreich MD,Makris JC,Reznikoff WS





Has Abstract


1991-11-01 00:00:00












  • Indirect selection of bacterial plasmids lacking identifiable phenotypic properties.

    abstract::A procedure is described that uses an indicator plasmid (pSC201) to identify cells in a bacterial population that have been co-transformed with a second plasmid lacking detectable phenotypic properties. Under appropriate conditions of indirect selection, between 50 and 85% of transformants carrying the indicator plasm...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kretschmer FJ,Chang AC,Cohen SN

    更新日期:1975-10-01 00:00:00

  • Identification of the Final Two Genes Functioning in Methanofuran Biosynthesis in Methanocaldococcus jannaschii.

    abstract:UNLABELLED:All methanofuran structural variants contain a basic core structure of 4-[N-(γ-l-glutamyl)-p-(β-aminoethyl)phenoxymethyl]-(aminomethyl)furan (APMF-Glu) but have different side chains depending on the source organism. Recently, we identified four genes (MfnA, MfnB, MfnC, and MfnD) that are responsible for the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Wang Y,Xu H,Jones MK,White RH

    更新日期:2015-09-01 00:00:00

  • Expression and characterization of Campylobacter jejuni benzoylglycine amidohydrolase (Hippuricase) gene in Escherichia coli.

    abstract::The basis for the difference between Campylobacter jejuni and Campylobacter coli is the presence and expression of the N-benzoylglycine amidohydrolase (hippuricase) gene only in C. jejuni. A pBR322 recombinant clone (pHIP-O) of C. jejuni TGH9011 capable of converting hippuric acid into benzoic acid and glycine, the ha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hani EK,Chan VL

    更新日期:1995-05-01 00:00:00

  • cis-acting sites in the transcript of the Bacillus subtilis trp operon regulate expression of the operon.

    abstract::Transcription of the trp operon of Bacillus subtilis is regulated by attenuation. A trpE'-'lacZ gene fusion preceded by the wild-type trp promoter-leader region was used to analyze regulation. Overproduction of the trp leader transcript in trans from a multicopy plasmid caused constitutive expression of the chromosoma...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kuroda MI,Henner D,Yanofsky C

    更新日期:1988-07-01 00:00:00

  • Role of NAD in regulating the adhE gene of Escherichia coli.

    abstract::The fermentative alcohol dehydrogenase of Escherichia coli is encoded by the adhE gene, which is induced under anaerobic conditions but repressed in air. Previous work suggested that induction of adhE might depend on NADH levels. We therefore directly measured the NAD+ and NADH levels for cultures growing aerobically ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Leonardo MR,Dailly Y,Clark DP

    更新日期:1996-10-01 00:00:00

  • Ultrastructural studies of sporulation in Streptomyces.

    abstract::This is the first study of sporogenesis in Streptomyces carried out on a relatively high number of species (seven) which allows us, using also previously published results, to establish a general picture of this process. In the sporogenesis of Streptomyces two basic stages can be considered: the sporulation septum syn...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hardisson C,Manzanal MB

    更新日期:1976-09-01 00:00:00


    abstract::Woody-Karrer, Pearl (Palo Alto Medical Research Foundation, Palo Alto, Calif.) and Joseph Greenberg. Resistance and cross resistance of Escherichia coli S mutants to the radiomimetic agent nitrofurazone. J. Bacteriol. 85:1208-1216. 1963.-Cross-resistance relationships are described for 73 mutants of Escherichia coli s...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章



    更新日期:1963-06-01 00:00:00

  • Limited role for the DsrA and RprA regulatory RNAs in rpoS regulation in Salmonella enterica.

    abstract::RpoS, the sigma factor of enteric bacteria that responds to stress and stationary phase, is subject to complex regulation acting at multiple levels, including transcription, translation, and proteolysis. Increased translation of rpoS mRNA during growth at low temperature, after osmotic challenge, or with a constitutiv...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Jones AM,Goodwill AG,Elliott T

    更新日期:2006-07-01 00:00:00

  • Effects of salts on the lethality of paraquat.

    abstract::Escherichia coli suffered 95 to 100% lethality when exposed to 1.0 mM paraquat for 30 min at 37 degrees C in aerobic nutrient broth medium but did not lose viability when the exposure was done in Vogel Bonner or tryptic soy yeast extract medium. Paraquat was, however, bacteriostatic in all of these media. Salts, added...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kitzler J,Fridovich I

    更新日期:1986-07-01 00:00:00

  • The phosphoenolpyruvate phosphotransferase system regulates Vibrio cholerae biofilm formation through multiple independent pathways.

    abstract::The bacterial phosphoenolpyruvate phosphotransferase system (PTS) is a highly conserved phosphotransfer cascade that participates in the transport and phosphorylation of selected carbohydrates and modulates many cellular functions in response to carbohydrate availability. It plays a role in the virulence of many bacte...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Houot L,Chang S,Pickering BS,Absalon C,Watnick PI

    更新日期:2010-06-01 00:00:00


    abstract::Romano, Antonio H. (University of Cincinnati, Cincinnati, Ohio), and Dorothy J. Geason. Pattern of sheath synthesis in Sphaerotilus natans. J. Bacteriol. 88:1145-1150. 1964.-Formation of the characteristic sheath of Sphaerotilus natans was followed by immunofluorescence. Fluorescent antisheath antibody was obtained by...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: ROMANO AH,GEASON DJ

    更新日期:1964-10-01 00:00:00

  • Protein kinase/phosphatase function correlates with gliding motility in Mycoplasma pneumoniae.

    abstract::Mycoplasma pneumoniae exhibits a novel form of gliding motility that is mediated by the terminal organelle, a differentiated polar structure. Given that genes known to be involved in gliding in other organisms are absent in M. pneumoniae, random transposon mutagenesis was employed to generate mutants with gliding-defi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Page CA,Krause DC

    更新日期:2013-04-01 00:00:00

  • Hydrogen-dependent nitrogenase activity and ATP formation in Rhizobium japonicum bacteroids.

    abstract::Rhizobium japonicum 122 DES bacteroids from soybean nodules possess an active H(2)-oxidizing system that recycles all of the H(2) lost through nitrogenase-dependent H(2) evolution. The addition of 72 muM H(2) to suspensions of bacteroids increased O(2) uptake 300% and the rate of C(2)H(2) reduction 300 to 500%. The op...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Emerich DW,Ruiz-Argüeso T,Ching TM,Evans HJ

    更新日期:1979-01-01 00:00:00

  • DnaK mutants defective in ATPase activity are defective in negative regulation of the heat shock response: expression of mutant DnaK proteins results in filamentation.

    abstract::Site-directed mutagenesis has previously been used to construct Escherichia coli dnaK mutants encoding proteins that are altered at the site of in vitro phosphorylation (J. S. McCarty and G. C. Walker, Proc. Natl. Acad. Sci. USA 88:9513-9517, 1991). These mutants are unable to autophosphorylate and are severely defect...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: McCarty JS,Walker GC

    更新日期:1994-02-01 00:00:00

  • The periplasmic, group III catalase of Vibrio fischeri is required for normal symbiotic competence and is induced both by oxidative stress and by approach to stationary phase.

    abstract::The catalase gene, katA, of the sepiolid squid symbiont Vibrio fischeri has been cloned and sequenced. The predicted amino acid sequence of KatA has a high degree of similarity to the recently defined group III catalases, including those found in Haemophilus influenzae, Bacteroides fragilis, and Proteus mirabilis. Ups...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Visick KL,Ruby EG

    更新日期:1998-04-01 00:00:00

  • Consequences of glycerol deprivation on the synthesis of membrane components in a glycerol auxotroph of Staphylococcus aureus.

    abstract::In a glycerol auxotroph of Staphylococcus aureus, the deprivation of glycerol affected the formation of certain membrane components. (i) There was synthesis of fatty acids at the predeprivation rate even though the fatty acids synthesized accumulated as free fatty acids rather than as esterified fatty acids; (ii) ther...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ray PH,Lillich TT,White DC

    更新日期:1972-10-01 00:00:00

  • Functional complementation between bacterial MDR-like export systems: colicin V, alpha-hemolysin, and Erwinia protease.

    abstract::The antibacterial protein Colicin V (ColV) is secreted from gram-negative bacteria by a signal sequence-independent pathway. The proteins that mediate the export of ColV share sequence similarities with components from other signal sequence-independent export systems such as those for alpha-hemolysin (Hly) and Erwinia...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Fath MJ,Skvirsky RC,Kolter R

    更新日期:1991-12-01 00:00:00

  • Phage-associated mutator phenotype in group A streptococcus.

    abstract::Defects in DNA mismatch repair (MMR) occur frequently in natural populations of pathogenic and commensal bacteria, resulting in a mutator phenotype. We identified a unique genetic element in Streptococcus pyogenes strain SF370 that controls MMR via a dynamic process of prophage excision and reintegration in response t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Scott J,Thompson-Mayberry P,Lahmamsi S,King CJ,McShan WM

    更新日期:2008-10-01 00:00:00

  • Vibrio cholerae O139 Bengal: combined physical and genetic map and comparative analysis with the genome of V. cholerae O1.

    abstract::A combined physical and genetic map of the genome of strain SG24 of Vibrio cholerae O139 Bengal, a novel non-O1 strain having epidemic potential, has been constructed by using the enzymes NotI, SfiI, and CeuI. The genome of SG24 is circular, and the genome size is about 3. 57 Mb. The linkages between 47 NotI and 32 Sf...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Khetawat G,Bhadra RK,Kar S,Das J

    更新日期:1998-09-01 00:00:00

  • Regulation of cytotoxicity by quorum-sensing signaling in Vibrio vulnificus is mediated by SmcR, a repressor of hlyU.

    abstract::Cytotoxicity is an important virulence determinant in the pathogenesis of Vibrio vulnificus, and two cytotoxins, RTX (encoded by rtxA1) and cytolysin/hemolysin (encoded by vvhA), have been identified in this organism. We showed that the quorum-sensing regulator LuxO controlled the cytotoxicity of this organism: a Δlux...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Shao CP,Lo HR,Lin JH,Hor LI

    更新日期:2011-05-01 00:00:00

  • Method for fingerprinting yeast cell wall mannans.

    abstract::Controlled acetolysis of yeast mannans yields mixtures of oligosaccharides with (1-->2) and (1-->3) linkages between the mannose units, whereas the less stable (1-->6) linkages of the polysaccharide backbone are cleaved. The "fingerprints," obtained by gel filtration of the oligosaccharide mixtures, can be used to dis...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kocourek J,Ballou CE

    更新日期:1969-12-01 00:00:00

  • Investigations of Dimethylglycine, Glycine Betaine, and Ectoine Uptake by a Betaine-Carnitine-Choline Transporter Family Transporter with Diverse Substrate Specificity in Vibrio Species.

    abstract::Fluctuations in osmolarity are one of the most prevalent stresses to which bacteria must adapt, both hypo- and hyperosmotic conditions. Most bacteria cope with high osmolarity by accumulating compatible solutes (osmolytes) in the cytoplasm to maintain the turgor pressure of the cell. Vibrio parahaemolyticus, a halophi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Gregory GJ,Dutta A,Parashar V,Boyd EF

    更新日期:2020-11-19 00:00:00

  • Architecture of the cell envelope of Chlamydia psittaci 6BC.

    abstract::The cysteine-rich envelope proteins of the elementary body form of chlamydiae are thought to be located in the outer membrane on the basis of their insolubility in the weak anionic detergent N-lauryl sarcosinate (Sarkosyl). We found, however, that the insolubility of the small (EnvA) and the large (EnvB) cysteine-rich...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Everett KD,Hatch TP

    更新日期:1995-02-01 00:00:00

  • Suppression of early competence mutations in Bacillus subtilis by mec mutations.

    abstract::Although competence normally develops only in glucose-minimal salts media, mecA and mecB mutations permit the expression of competence and of late competence genes in complex media as well (D. Dubnau and M. Roggiani, J. Bacteriol. 172:4048-4055, 1990). The expression of late competence genes is dependent on the produc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Roggiani M,Hahn J,Dubnau D

    更新日期:1990-07-01 00:00:00

  • The OxyR regulon in nontypeable Haemophilus influenzae.

    abstract::Nontypeable Haemophilus influenzae (NTHi) is a gram-negative bacterium and a common commensal organism of the upper respiratory tract in humans. NTHi causes a number of diseases, including otitis media, sinusitis, conjunctivitis, exacerbations of chronic obstructive pulmonary disease, and bronchitis. During the course...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Harrison A,Ray WC,Baker BD,Armbruster DW,Bakaletz LO,Munson RS Jr

    更新日期:2007-02-01 00:00:00

  • FepA with globular domain deletions lacks activity.

    abstract::TonB-gated transporters have beta-barrels containing an amino-terminal globular domain that occludes the interior of the barrel. Mutations in the globular domain prevent transport of ligands across the outer membrane. Surprisingly, FepA with deletions of the globular domain (amino acids 3 to 150 and 17 to 150) was pre...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Vakharia HL,Postle K

    更新日期:2002-10-01 00:00:00


    abstract::Nishida, S. (Kanazawa University, Kanazawa, Japan), and G. Nakagawara. Isolation of toxigenic strains of Clostridium novyi from soil. J. Bacteriol. 88:1636-1640. 1964.-The cultural conditions for toxin production by Clostridium novyi were investigated, and the optimal constituents of a proper medium were determined. A...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章



    更新日期:1964-12-01 00:00:00

  • Recruitment of the ParG segregation protein to different affinity DNA sites.

    abstract::The segrosome is the nucleoprotein complex that mediates accurate plasmid segregation. In addition to its multifunctional role in segrosome assembly, the ParG protein of multiresistance plasmid TP228 is a transcriptional repressor of the parFG partition genes. ParG is a homodimeric DNA binding protein, with C-terminal...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Zampini M,Derome A,Bailey SE,Barillà D,Hayes F

    更新日期:2009-06-01 00:00:00

  • Capsular polysaccharide production in Enterococcus faecalis and contribution of CpsF to capsule serospecificity.

    abstract::Many bacterial species produce capsular polysaccharides that contribute to pathogenesis through evasion of the host innate immune system. The gram-positive pathogen Enterococcus faecalis was previously reported to produce one of four capsule serotypes (A, B, C, or D). Previous studies describing the four capsule serot...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Thurlow LR,Thomas VC,Hancock LE

    更新日期:2009-10-01 00:00:00

  • Control of EpsE, the phosphoglycosyltransferase initiating exopolysaccharide synthesis in Streptococcus thermophilus, by EpsD tyrosine kinase.

    abstract::We studied the roles of Streptococcus thermophilus phosphogalactosyltransferase (EpsE) (the priming enzyme), tyrosine kinase (EpsD), phosphatase (EpsB), and a membrane-associated protein with no known biochemical function (EpsC) in exopolysaccharide (EPS) synthesis. These proteins are well-conserved among bacteria and...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Minic Z,Marie C,Delorme C,Faurie JM,Mercier G,Ehrlich D,Renault P

    更新日期:2007-02-01 00:00:00